Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 660,624 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583660,6240GC99.6% 571.5 / NA 245intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (131/113);  minor base T (1/0);  total (132/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583660,6250CG99.2% 560.7 / NA 245intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (132/111);  total (132/113)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583660,6260TA99.6% 576.9 / NA 245intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (132/111);  minor base G (0/1);  total (132/113)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

GCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGAGCATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATGCTCCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGA  >  CP042583/660347‑660904
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                       
gCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTg                                                                                                                                                                                                                                                                                <  1:143411/289‑1 (MQ=255)
    tgGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTgcgc                                                                                                                                                                                                                                                                             >  2:1651190/1‑288 (MQ=255)
     gggCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGcc                                                                                                                                                                                                                                                                            <  1:723154/288‑1 (MQ=255)
      ggCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCt                                                                                                                                                                                                                                                                           >  2:1911909/1‑288 (MQ=255)
                   ccGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTc                                                                                                                                                                                                                                                            <  1:223880/289‑1 (MQ=255)
                      tGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCaa                                                                                                                                                                                                                                                          >  2:390590/1‑288 (MQ=255)
                      tGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCaa                                                                                                                                                                                                                                                          >  2:1882255/1‑288 (MQ=255)
                            aGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATCCCACGACGTTTTACATTGACCAAACTGACTCCTCAACCCATTT‑CTGCaaaacgt                                                                                                                                                                                                                                                     >  2:856171/1‑283 (MQ=255)
                                        gCGATCAAACCTGGCCTGTGGTTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCTCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACTTTTACTTTGCCTGtt                                                                                                                                                                                                                                        >  2:1107496/1‑288 (MQ=255)
                                        gCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGtt                                                                                                                                                                                                                                        >  1:807554/1‑288 (MQ=255)
                                         cGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGAAATCATTAAAGTTATCCCCGAATGCCACGACGTCTTCCATCGACCAACTCTTCCACTCCACCCATTT‑CTTCAAACGTTTACTTTTGCCGTTg                                                                                                                                                                                                                                       >  2:1624433/1‑288 (MQ=255)
                                            tCAAACCTGGCCTGTGGCTGTACGCGCAAAGTAAAAGGGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTAGAAATGAACTGAGCAATGCTGTCGGTGGTGTTATCACCAATCACGATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTgccg                                                                                                                                                                                                                                    <  2:243148/288‑1 (MQ=255)
                                             cAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTgccgcc                                                                                                                                                                                                                                  <  1:1882255/289‑1 (MQ=255)
                                             cAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTgccgcc                                                                                                                                                                                                                                  <  2:787804/289‑1 (MQ=255)
                                             cAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTgccgcc                                                                                                                                                                                                                                  <  1:707059/289‑1 (MQ=255)
                                                      ccTGTGGCTGTCCGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCTCCGCGTGCAat                                                                                                                                                                                                                         >  1:102401/1‑289 (MQ=255)
                                                       cTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACC‑TTGCTGTTGCCGCCGCGTGCAata                                                                                                                                                                                                                        <  1:469097/288‑1 (MQ=255)
                                                          tgGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCACTATcc                                                                                                                                                                                                                     >  2:340121/1‑288 (MQ=255)
                                                                 atacgcAAATTAAAAGCGTGTCAATAACCGCCGAATTAAATGAGGTG‑TCTATACATGAACGGGGCAATGCTGTCGGGGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTGTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTAATAGCGGCTTCCAGCATACAGATAACATCAAAGTTATCGCCGAAGGCCACGACGTTTTCCATCGACCAACCTAGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGTAATATCAACCTGAt                                                                                                                                                                                                              <  2:1565036/286‑1 (MQ=255)
                                                                 aCGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGAt                                                                                                                                                                                                              <  1:896063/289‑1 (MQ=255)
                                                                 aCGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGAt                                                                                                                                                                                                              <  2:1417621/289‑1 (MQ=255)
                                                                 aCGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGAt                                                                                                                                                                                                              <  1:90167/289‑1 (MQ=255)
                                                                 aCGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGAt                                                                                                                                                                                                              <  1:1474040/289‑1 (MQ=255)
                                                                  cgcgCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTCTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATc                                                                                                                                                                                                             >  1:929882/1‑289 (MQ=255)
                                                                             aaaGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGAcc                                                                                                                                                                                                  <  1:41046/289‑1 (MQ=255)
                                                                             aaaGTGTGTCGATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGAcc                                                                                                                                                                                                  <  1:1911909/289‑1 (MQ=255)
                                                                                  gtgtcgaTAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTca                                                                                                                                                                                             <  1:1720793/283‑1 (MQ=255)
                                                                                   tgtcgaTAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTc                                                                                                                                                                                                                                                            <  2:1390587/220‑1 (MQ=255)
                                                                                   tgtcgaTAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGTGTGCAATATCAACCTGATCGTGCCAGGACCATTcac                                                                                                                                                                                            >  1:36536/6‑289 (MQ=255)
                                                                                   tgtcgaTAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTca                                                                                                                                                                                             >  2:1330684/6‑288 (MQ=255)
                                                                                   tgtcgaTAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTca                                                                                                                                                                                             <  2:1197199/283‑1 (MQ=255)
                                                                                   tgtcgaTAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACCCCTGTCCCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CTTCACACGTTTACCTTTGCTGTTGCTGCCGCCTGCAATAACAACCTCATCTTACCAgcgactgtca                                                                                                                                                                                             >  2:1274133/6‑279 (MQ=255)
                                                                                         tAACCTCCTGTTTAAATCAAGTGCGGTA‑AAATGAACTGGGCAAGTCTGGCGGTTGAGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGGTATCATTAAAGTTATCGCCGTATGCCACGACGTTGTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCa                                                                                                                                                                                      <  2:1996010/289‑1 (MQ=255)
                                                                                               ccTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGAt                                                                                                                                                                                                              >  1:656927/1‑259 (MQ=255)
                                                                                               ccTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGAt                                                                                                                                                                                                              <  2:656927/259‑1 (MQ=255)
                                                                                               ccTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGa                                                                                                                                                                                                    >  1:1841354/1‑269 (MQ=255)
                                                                                               ccTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCa                                                                                                                                                                                >  1:125233/1‑289 (MQ=255)
                                                                                                             gTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGATCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCCCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACCCCCCAGTCCCAGTTCATGTTCGAcc                                                                                                                                                                  >  2:222833/1‑288 (MQ=255)
                                                                                                              gtgccgataAATGACCTGGGCAATGCTGTCGGGGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGAc                                                                                                                                                                   <  2:125233/282‑1 (MQ=255)
                                                                                                                 gCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGGTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCTCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACAt                                                                                                                                                                 >  1:123387/1‑287 (MQ=255)
                                                                                                                            aCTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGAt                                                                                                                                                                                                              <  2:1488352/231‑1 (MQ=255)
                                                                                                                             cTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGAt                                                                                                                                                                                                              >  1:1989925/1‑230 (MQ=255)
                                                                                                                               gggCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAAtgct                                                                                                                                                  >  1:178108/1‑288 (MQ=255)
                                                                                                                                ggCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAAtgctgc                                                                                                                                                >  1:1813322/1‑289 (MQ=255)
                                                                                                                                ggCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGAGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTg                                                                                                                                                                                                                             <  1:776764/212‑1 (MQ=255)
                                                                                                                                ggCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGAGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTg                                                                                                                                                                                                                             >  2:776764/1‑212 (MQ=255)
                                                                                                                                       cTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCAt                                                                                                                                                                          <  1:955958/256‑1 (MQ=255)
                                                                                                                                           cGGTGGTGTTATCCCCAAACACAATGTTGGCGCGCCCTTTTACCACGTCATCGGCGCTGCCCATCACCACGCCTGTACCAGCGGCTTCCAGCATATTAATATCATTAAAGTTATCGCCCAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCGCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCa                                                                                                                                      <  2:1238804/288‑1 (MQ=255)
                                                                                                                                               ggtgTTATCACCAATCACAATGTTGGCGCGCGCTTTTAACGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTCAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCACACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTTATCGTGCCAGGACCATTCACACTCCAGTCCCAGTCCATGTTAGACATGCTTACCAAAATGCCGCAATTGCGGCAGGTCa                                                                                                                                 >  2:433570/1‑289 (MQ=255)
                                                                                                                                                 tgtTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGAt                                                                                                                                                                                                              <  2:921955/210‑1 (MQ=255)
                                                                                                                                                 tgtTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGAc                                                                                                                                                                                                   <  2:879764/221‑1 (MQ=255)
                                                                                                                                                  tttATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCg                                                                                                                              <  1:643108/288‑1 (MQ=255)
                                                                                                                                                  gtTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGCCATGTTTACCAAAATGCTGCAATTACGGCAgcgactcg                                                                                                                              >  2:354776/1‑282 (MQ=255)
                                                                                                                                                        aCCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAACTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTTTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCCTCAATTGCGGCAGGTCAtcgttcgt                                                                                                                         >  2:914252/1‑288 (MQ=255)
                                                                                                                                                           aaTCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTTCCGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTCCgaccg                                                                                                                      >  2:1837322/1‑285 (MQ=255)
                                                                                                                                                           aaTCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCCAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCCCACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAACTGCTGCAATTGCCGCAGGTCATCGTTCGTCAgg                                                                                                                     >  2:1852574/1‑289 (MQ=255)
                                                                                                                                                           aaTCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAgg                                                                                                                     >  1:850460/1‑289 (MQ=255)
                                                                                                                                                                caATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGaa                                                                                                                <  2:84104/289‑1 (MQ=255)
                                                                                                                                                                   tGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCCACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTTCCGCAGGTCATCGTTCGTCAGGGCTAACt                                                                                                              >  2:1039593/1‑288 (MQ=255)
                                                                                                                                                                   tGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAgg                                                                                                                                                                                                     <  1:1384544/201‑1 (MQ=255)
                                                                                                                                                                   tGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAgg                                                                                                                                                                                                     >  2:1384544/1‑201 (MQ=255)
                                                                                                                                                                       ggCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCCGGTCATCGTGCGTCAGGGCGAACTTCCAt                                                                                                         >  2:511268/1‑289 (MQ=255)
                                                                                                                                                                       ggCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCg                                                                                                                  <  1:1413649/280‑1 (MQ=255)
                                                                                                                                                                       ggCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGAAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGATGCAATTGCGGCAGGTCATCTTTCGTCAGGGCGAACTTCCAt                                                                                                         >  1:1033286/1‑289 (MQ=255)
                                                                                                                                                                         cgcgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATa                                                                                                        <  1:1330684/288‑1 (MQ=255)
                                                                                                                                                                         cgcgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATa                                                                                                        <  1:1283624/288‑1 (MQ=255)
                                                                                                                                                                         cgcgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATa                                                                                                        <  1:1649596/288‑1 (MQ=255)
                                                                                                                                                                         cgcgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAACTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTTTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATa                                                                                                        <  1:914252/288‑1 (MQ=255)
                                                                                                                                                                           cgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACg                                                                                                      >  1:383057/1‑288 (MQ=255)
                                                                                                                                                                             cgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCg                                                                                                   >  2:1761974/1‑289 (MQ=255)
                                                                                                                                                                                        cgTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACttgtt                                                                                         >  1:1742765/1‑288 (MQ=255)
                                                                                                                                                                                          tCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCg                                                                                                   >  1:1503616/1‑276 (MQ=255)
                                                                                                                                                                                           cATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTgcg                                                                                      <  1:1239454/288‑1 (MQ=255)
                                                                                                                                                                                           cATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTgcg                                                                                      <  1:511268/288‑1 (MQ=255)
                                                                                                                                                                                           cATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTgcg                                                                                      <  1:267196/288‑1 (MQ=255)
                                                                                                                                                                                                   ttGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCa                                                                              >  1:584573/1‑288 (MQ=255)
                                                                                                                                                                                                     gCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGcc                                                                           >  1:402111/1‑289 (MQ=255)
                                                                                                                                                                                                                      tACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTTCGTCAAGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTtgtgt                                                          >  1:464488/1‑289 (MQ=255)
                                                                                                                                                                                                                            cGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATg                                                                                                                                                                         <  1:1518677/172‑1 (MQ=255)
                                                                                                                                                                                                                            cGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCCTTCGTCAGGGCGAACTTCCATACGGCGTTAACTTGGTGCGCCGTTTCAGCCAGAGAAGCGCCTTGTCGGAAAg                                                     >  2:808626/1‑288 (MQ=255)
                                                                                                                                                                                                                                   cAGCATACTGATATCATTAAAGTGATCGCCGAATGCCACGACGGTTTCCATCGAACAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGGGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACg                                              <  1:1114183/288‑1 (MQ=255)
                                                                                                                                                                                                                                        tACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGtt                                         >  1:1219900/1‑288 (MQ=255)
                                                                                                                                                                                                                                             atatCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTGAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCcggcg                                   >  2:700501/1‑289 (MQ=255)
                                                                                                                                                                                                                                             atatCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAat                                                                                                                                                                                                                         <  1:1728130/107‑1 (MQ=255)
                                                                                                                                                                                                                                             atatCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAggg                                                                                                                    >  1:669859/1‑208 (MQ=255)
                                                                                                                                                                                                                                             atatCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCcggcg                                   >  2:1170442/1‑289 (MQ=255)
                                                                                                                                                                                                                                                tCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCa                                <  2:1511199/289‑1 (MQ=255)
                                                                                                                                                                                                                                                tCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCa                                >  1:1292749/1‑289 (MQ=255)
                                                                                                                                                                                                                                                  aTTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTgccgcc                                                                                                                                                                                                                                  <  2:1012177/93‑1 (MQ=255)
                                                                                                                                                                                                                                                  aTTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTgccgcc                                                                                                                                                                                                                                  >  1:1012177/1‑93 (MQ=255)
                                                                                                                                                                                                                                                          tttCGCCGACTGCCACGACGGTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGGGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTgcgc                      <  2:1292749/287‑1 (MQ=255)
                                                                                                                                                                                                                                                          tATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTgcgc                      <  1:1160493/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          tATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTgcgc                      <  1:1852574/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          tATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTgcgc                      <  2:1742765/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           aTCGCCGAATGCCACTACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGCCAAACGTTTACCTTTGCTTTTGCCGCCGCGTGCAATATCAACCTGAGCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGcc                     <  1:856171/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           aTCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTcc                                                                                                                                                                                  >  2:1545684/1‑132 (MQ=255)
                                                                                                                                                                                                                                                           aTCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATa                                                                                                        >  1:1552037/1‑206 (MQ=255)
                                                                                                                                                                                                                                                           aTCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGTGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTgcgc                      >  1:94994/1‑288 (MQ=255)
                                                                                                                                                                                                                                                           aTCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAAAAGCGACTTGGGGTAAAGTCGGACGCTGTTCCGGCGGCAGGGTTGgcgc                      >  2:590258/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                         cgacgTTTTCCATCGACCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGAGTTAACTTGTTGCGCCGTTTCAGCCAGGGAAGCGACTTGTGTGAAAGTCGGAAGCTGTTCCGGCGGTCGGTGTTGGGCCCAGTTAGCtgtggg       >  2:687497/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                       
GCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGAGCATAACCGCCTGATTAAATCAGGTG‑GCTATAAATGAACTGGGCAATGCTGTCGGTGGTGTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATGCTCCAACCTTGCGCCTCAACCCATTT‑CGTCAAACGTTTACCTTTGCTGTTGCCGCCGCGTGCAATATCAACCTGATCGTGCCAGGACCATTCACACTCCAGTCCCAGTTCATGTTCGACATGCTTACCAAAATGCTGCAATTGCGGCAGGTCATCGTGCGTCAGGGCGAACTTCCATACGGCGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGA  >  CP042583/660347‑660904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A