Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,455,450 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,455,4500AT100.0% 591.5 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (124/124);  total (124/124)
*CP0425831,455,4510GC99.6% 583.3 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (124/123);  minor base A (0/1);  total (124/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,455,4520TA99.2% 581.7 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (124/121);  minor base C (0/2);  total (124/124)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GCCCAAATATGCGATGGCTTTTGCTGGTATGAGTGAAGCTGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCAGCTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCAGTGCAGCTGGCGGCATTTTACAGCTCGGCGTTAGCG‑TCATGAGTGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGAGCAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATA  >  CP042583/1455170‑1455720
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                            
gCCCAAATATGCGATGGCTTTTGCTGGTATGTCAGAAGCTGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGttt                                                                                                                                                                                                                                                                        >  1:644230/1‑289 (MQ=255)
             ctGGCTTTTGCTGGTATGTCAGAAGCTGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACATCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAaaagc                                                                                                                                                                                                                                                            <  1:234673/287‑1 (MQ=255)
                gCTTTTGCTGGTATGTCAGAAGCTGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAaaagcaaa                                                                                                                                                                                                                                                         >  1:343886/1‑288 (MQ=255)
                   tttGCTGGTATGTAGGAAGCTGGGGCATTAAATACCATCTCTTATTACAGTATGGGCTCTGTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCa                                                                                                                                                                                                                                                     <  2:1675203/289‑1 (MQ=255)
                           tatgtcataacgtGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              <  2:1407449/276‑1 (MQ=255)
                           tatgtcagAAGCTGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              <  2:157829/281‑1 (MQ=255)
                           tatgtcagAAGCTGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              <  2:1255057/281‑1 (MQ=255)
                           tatgtcagAAGCTGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              <  1:1322243/281‑1 (MQ=255)
                                cataaGCTGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTAt                                                                                                                                                                                                                                        <  1:1948731/286‑1 (MQ=255)
                                cagAAGCTGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTAt                                                                                                                                                                                                                                        <  1:1961945/287‑1 (MQ=255)
                                          ggCATTAAAAACCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATatgat                                                                                                                                                                                                                               <  2:520999/288‑1 (MQ=255)
                                                   aaCCATCTCTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGa                                                                                                                                                                                                                     <  1:4391/289‑1 (MQ=255)
                                                        tctcTTATTACAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTgg                                                                                                                                                                                                                 >  2:129533/1‑288 (MQ=255)
                                                              attaCAGTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACtt                                                                                                                                                                                                          <  2:1199567/289‑1 (MQ=255)
                                                                    gTATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCCCCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTAt                                                                                                                                                                                                    >  1:1429585/1‑289 (MQ=255)
                                                                     tATGGGCTCGTTTGTCTTTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATACCAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTAtt                                                                                                                                                                                                   <  1:913079/289‑1 (MQ=255)
                                                                     tATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGcc                                                                                                                                                                                                                                                      >  1:1497785/1‑238 (MQ=255)
                                                                     tATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGcc                                                                                                                                                                                                                                                      <  2:1497785/238‑1 (MQ=255)
                                                                     tATGGGCTCGTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTAtt                                                                                                                                                                                                   >  2:22485/1‑289 (MQ=255)
                                                                      atgggctcgttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              <  1:376167/236‑1 (MQ=255)
                                                                      atgggctcgttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              >  2:376167/10‑245 (MQ=255)
                                                                      atgggctcgttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTAtt                                                                                                                                                                                                   >  1:1883362/10‑288 (MQ=255)
                                                                      atgggctcgttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCCCGGCGTTTCGG‑TCATGTCAGCGTTTTTTCCCAAAAGCAAAGCTAAAGTCACCAGTATTTATACGAGGATGGGCGGACTGGCTACCTTTTTTAtt                                                                                                                                                                                                   >  1:753427/10‑288 (MQ=255)
                                                                       tgggctcgttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGTTGGACTGGCTAACTTTGTTATTc                                                                                                                                                                                                  >  1:1576946/9‑288 (MQ=255)
                                                                        gggctcgttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              <  1:1649398/236‑1 (MQ=255)
                                                                        gggctcgttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              >  2:1649398/8‑243 (MQ=255)
                                                                          gctcgttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTcc                                                                                                                                                                                                 >  1:1227989/6‑286 (MQ=255)
                                                                          gctcgttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACt                                                                                                                                                                                              >  2:775213/6‑289 (MQ=255)
                                                                               ttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              >  1:848775/1‑236 (MQ=255)
                                                                               ttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGAtt                                                                                                                                                                                          >  1:1738264/1‑288 (MQ=255)
                                                                               ttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGAtt                                                                                                                                                                                          >  1:1371080/1‑288 (MQ=255)
                                                                               ttGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGAATGGCTAACTTGGTTTTTCCAATGAtt                                                                                                                                                                                          >  2:1903136/1‑288 (MQ=255)
                                                                                tGGTCTTTTTCTTTATTTTTGCCGCCCTACTGAAAAAAATGGTCCGGCCCAGCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTAc                                                                                                                                                                                        <  2:478839/289‑1 (MQ=255)
                                                                                      gtgtCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGAATTtat                                                                                                                                                                                                                                    <  1:583672/239‑1 (MQ=255)
                                                                                      gtgtCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGAATTtat                                                                                                                                                                                                                                    >  2:583672/1‑239 (MQ=255)
                                                                                       tgtCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTAt                                                                                                                                                                                 <  2:927565/289‑1 (MQ=255)
                                                                                        gtCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTa                                                                                                                                                                                  >  1:1685479/1‑287 (MQ=255)
                                                                                        gtCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTa                                                                                                                                                                                  >  2:1547278/1‑287 (MQ=255)
                                                                                             tATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTTGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTgtcg                                                                                                                                                                           >  1:1085269/1‑286 (MQ=255)
                                                                                                 tttGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              <  2:1185081/218‑1 (MQ=255)
                                                                                                 tttGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTgtcgaaca                                                                                                                                                                       >  2:8230/1‑282 (MQ=255)
                                                                                                 tttGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCAAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              >  1:465499/1‑218 (MQ=255)
                                                                                                      cGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGc                                                                                                                                                                  <  1:775213/289‑1 (MQ=255)
                                                                                                      cGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGc                                                                                                                                                                  <  1:320400/289‑1 (MQ=255)
                                                                                                         actactGAACAAAATGGTCCGGCCAATCTGTGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGACATTTTCTACCTGTACCCTTCGCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCATCTGGCTGCATTTTACAGCTCGGTGTTTCGG‑TCATGTCATAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGTGTATCTGTCGAACATCGGCCTg                                                                                                                                                               <  2:804378/289‑1 (MQ=255)
                                                                                                         actactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTg                                                                                                                                                               <  1:22485/289‑1 (MQ=255)
                                                                                                         actactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCAGCCTg                                                                                                                                                               <  1:1708771/289‑1 (MQ=255)
                                                                                                           tactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCa                                                                                                                                                             <  1:1196309/289‑1 (MQ=255)
                                                                                                                          tCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTtc                                                                                                                                               >  1:491284/1‑288 (MQ=255)
                                                                                                                                             tGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATCTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCgct                                                                                                                            >  1:878097/1‑288 (MQ=255)
                                                                                                                                                 tttAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGctgctg                                                                                                                        >  1:1287336/1‑288 (MQ=255)
                                                                                                                                                   tAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGcc                                                                                                                     <  2:685615/289‑1 (MQ=255)
                                                                                                                                                         tGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTGTCTGTGGAACATCGGCTGGCAATCTAATCTTGTTTCCGTTTTTACTTTGCCCTTGCTGGCCCttctc                                                                                                               >  2:1026861/1‑285 (MQ=255)
                                                                                                                                                         tGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGAtt                                                                                                               >  1:1132552/1‑289 (MQ=255)
                                                                                                                                                           cACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAc                                                                                                                                                                                                                                              <  1:957366/160‑1 (MQ=255)
                                                                                                                                                           cACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTa                                                                                                              >  2:368401/1‑288 (MQ=255)
                                                                                                                                                           cACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTa                                                                                                              >  1:710743/1‑288 (MQ=255)
                                                                                                                                                              tGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGc                                                                                                          <  2:95438/289‑1 (MQ=255)
                                                                                                                                                                          cagcagCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGtttt                                                                                              >  1:1837922/1‑289 (MQ=255)
                                                                                                                                                                            gcagcCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTg                                                                                                                                                               >  2:1279900/1‑222 (MQ=255)
                                                                                                                                                                             cagcCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTa                                                                                            <  2:1127796/288‑1 (MQ=255)
                                                                                                                                                                               gcCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCAcc                                                                                                                                                                                                                                             <  1:1514792/141‑1 (MQ=255)
                                                                                                                                                                                          ccTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACcgc                                                                              >  1:102571/1‑289 (MQ=255)
                                                                                                                                                                                            tGTACCCTTCTCCACTGGTGTGCAATGCCGCAGCATTTGTTATCGGTTTCACAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATGTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACAGTGTATCTGTCAAACATGGGCCTGCAATATATAATTGTTCTCGATTTTTCTTTCGCGCTGCTGGCCCTGATTCCAGCAATTTTTGTTTTTAACCTCTATTAcgcctt                                                                            >  2:769036/1‑284 (MQ=255)
                                                                                                                                                                                             gTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGtt                                                                           <  2:1837922/289‑1 (MQ=255)
                                                                                                                                                                                              tACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGttt                                                                          >  1:901107/1‑289 (MQ=255)
                                                                                                                                                                                               aCCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGtttt                                                                         >  2:107422/1‑289 (MQ=255)
                                                                                                                                                                                                    tctcCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCctta                                                                    >  1:1587560/1‑289 (MQ=255)
                                                                                                                                                                                                    tctcCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCatta                                                                    >  1:1579702/1‑289 (MQ=255)
                                                                                                                                                                                                     ctcCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATatgat                                                                                                                                                                                                                               <  2:452968/133‑1 (MQ=255)
                                                                                                                                                                                                        cACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTCCTGGCCCTGATTCCCGCAATTATTGTTTTTATCCGTTATCACCGCGTTTTCcttctt                                                                  >  2:1580900/1‑287 (MQ=255)
                                                                                                                                                                                                             gtgtGCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGaaa                                                           >  1:49668/1‑289 (MQ=255)
                                                                                                                                                                                                              tgtgCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAGGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTAcc                                                                                >  2:971046/1‑267 (MQ=255)
                                                                                                                                                                                                              tgtgCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGaaa                                                           <  1:368401/288‑1 (MQ=255)
                                                                                                                                                                                                                tgCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTCTTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGTGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCTGCTATTACCGCGTTTTCATCATTCCTGAAAttg                                                        >  2:1110763/1‑289 (MQ=255)
                                                                                                                                                                                                                tgCAATGCCGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCa                                                                                                                                                             <  2:312582/188‑1 (MQ=255)
                                                                                                                                                                                                                       ccGGAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTGCATTATTCCTGAAAATTATGTGCg                                                 >  2:1387149/1‑289 (MQ=255)
                                                                                                                                                                                                                          gAGCCTTTGTTATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTa                                                                                            <  2:1803938/243‑1 (MQ=255)
                                                                                                                                                                                                                                    tATCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGt                                    <  1:1547278/289‑1 (MQ=255)
                                                                                                                                                                                                                                     aTCGGTTTCTCAGCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTa                                   <  2:431208/289‑1 (MQ=255)
                                                                                                                                                                                                                                              tcagCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCa                          <  1:1149099/286‑1 (MQ=255)
                                                                                                                                                                                                                                              tcagCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCa                          <  1:820228/286‑1 (MQ=255)
                                                                                                                                                                                                                                              tcagCAGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCa                          <  2:650642/286‑1 (MQ=255)
                                                                                                                                                                                                                                                ggccgCTGGCGGCATTTTACAGGTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCAcc                        <  2:234489/285‑1 (MQ=255)
                                                                                                                                                                                                                                                cacAGCTGGCCGCATTTTACAGGCCGGCGTTTTGG‑TCATTTCAGAGGTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCAcc                        <  2:1939401/287‑1 (MQ=255)
                                                                                                                                                                                                                                                   cgCTGGCGGAGTTTTACATGTCGGCGTTTCGG‑TCATTTACGAGTTTTTTCCCCAAAGCAAAGCCAAAGTCACCAGTATTTATCTGATGATGGGTGGACTGGCTAACTTTGTTATTCCTCTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCAcc                        <  2:1519522/285‑1 (MQ=255)
                                                                                                                                                                                                                                                   aGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCAcc                        <  1:1266861/286‑1 (MQ=255)
                                                                                                                                                                                                                                                   aGCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCAcc                        <  1:143841/286‑1 (MQ=255)
                                                                                                                                                                                                                                                    gCTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATTTATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGt                    >  1:940998/1‑289 (MQ=255)
                                                                                                                                                                                                                                                     cTGGCGGCATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGtt                   >  1:130563/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      tGGCGGCATTGTCAAGCTCGGTGTT‑GCGCTCAGGTCGGAGTTTTTTCCCAAAAGCAATGCCAAAGTCACCATTATTTTTTTGATGATGCGTGTACTGTCTGAATTTCTTATGCCACTGATTACCGGTGATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCCGCTGGCCCTGATTACCGCCATTATTGTTTTTATCCGCTAGTACCGCGGTTTCATTATTCCTGATAATGATGTGCGGTTTGGCGATCGTTAATTTTGCACCCGGtt                   <  2:332568/288‑1 (MQ=255)
                                                                                                                                                                                                                                                       ggcggcATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTaa                 <  2:1452136/289‑1 (MQ=255)
                                                                                                                                                                                                                                                         cggcATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTaaa                <  1:1110763/288‑1 (MQ=255)
                                                                                                                                                                                                                                                          ggcATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCattatt                                                                  >  1:673070/1‑237 (MQ=255)
                                                                                                                                                                                                                                                          ggcATTTTACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAaca              <  2:1336208/289‑1 (MQ=255)
                                                                                                                                                                                                                                                               tttACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAAtt         >  1:1819946/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               tttACAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAAtt         <  2:901107/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aCAGCTCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTaa       >  1:1803246/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                       tCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCa    <  2:490270/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tCGGCGTTTCGG‑TCATGTCAGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATa  <  2:1738264/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                            
GCCCAAATATGCGATGGCTTTTGCTGGTATGAGTGAAGCTGAGGCATTAAAAACCATCTCTTATTACAGTATGGGCAGCTTGGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAATGCCGGAGCCTTTGTTATCGGTTTCAGTGCAGCTGGCGGCATTTTACAGCTCGGCGTTAGCG‑TCATGAGTGAGTTTTTTCCCAAAAGCAAAGCCAAAGTCACCAGTATTTATATGATGATGGGTGGACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGAGCAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATA  >  CP042583/1455170‑1455720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A