Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,465,201 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,465,2010AT100.0% 566.6 / NA 239intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (133/106);  total (133/106)
*CP0425831,465,2020GC100.0% 562.8 / NA 239intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (133/106);  total (133/106)
*CP0425831,465,2030TA99.6% 565.7 / NA 239intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (132/106);  minor base C (1/0);  total (133/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

TAATCATGCATCCCACAGGCCCGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTAGCCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCAAGCTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAAAGTGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCACCTCTTCCCT  >  CP042583/1464923‑1465481
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                      
tAATCATGCATCCCACAGGCCCGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCACCATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGatc                                                                                                                                                                                                                                                                                 <  1:1679073/288‑1 (MQ=255)
   tcctGCATCCCACAGGCCCGCATCTCGGGCCTTATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTACCGTTTAACTAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGTCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGatcgcat                                                                                                                                                                                                                                                                             <  1:1166250/286‑1 (MQ=255)
       gCATCCCACAGGCCCGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCCCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGAtcgcatcgca                                                                                                                                                                                                                                                                         >  1:1631883/1‑289 (MQ=255)
        cATCCCACAGGCCCGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATcgcatcgcat                                                                                                                                                                                                                                                                        >  1:1076553/1‑289 (MQ=255)
        cATCCCACAGGCCCGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCCATcgcatcgcat                                                                                                                                                                                                                                                                        >  2:1339368/1‑289 (MQ=255)
         aTCCCACAGGCCCGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGAGGTTAGCGAA‑GGGTATTGAATCAGGCAAGCGCATCGCAtt                                                                                                                                                                                                                                                                       >  2:179521/1‑289 (MQ=255)
           cccACAGGCCCGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTc                                                                                                                                                                                                                                                                     >  1:823466/1‑289 (MQ=255)
            ccccaGGCCCGCATCTCGTGCCTGATGTTTTGTTGCGAGAGTCCAACATGGAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCa                                                                                                                                                                                                                                                                    <  2:619013/286‑1 (MQ=255)
            ccACAGGCCCGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCa                                                                                                                                                                                                                                                                    <  2:59022/289‑1 (MQ=255)
            ccACAGGCCCGAATCTCGGGGCTGATGTTCTGTTTCGGGAGTCCAACATGGAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCa                                                                                                                                                                                                                                                                    <  2:1483142/289‑1 (MQ=255)
             cacaGGCCCGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCa                                                                                                                                                                                                                                                                    >  1:662853/1‑288 (MQ=255)
                     cGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCctg                                                                                                                                                                                                                                                            >  1:380456/1‑288 (MQ=255)
                       cATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCTTACCCCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCctggct                                                                                                                                                                                                                                                         >  2:1260885/1‑289 (MQ=255)
                           tcGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGgtg                                                                                                                                                                                                                                                     <  1:1887117/289‑1 (MQ=255)
                            cGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGgtgt                                                                                                                                                                                                                                                    >  1:1363396/1‑289 (MQ=255)
                            cGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTCCATCGTACACCTATAGCACGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCAATCGAACCGCATTCCAACTGCctggcgggggg                                                                                                                                                                                                                                                    >  2:917335/1‑286 (MQ=255)
                            cGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGGGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCCCGATCACGCCGCGAGCTGTCTGGCCAACTGGATGTTAGCGAA‑GGGTGTTGAATCCGGCGGTCGCATCGCATTCCAACTGCCCTGCTGgttt                                                                                                                                                                                                                                                    >  2:272136/1‑287 (MQ=255)
                                 ctgtggtcctttttCGAGAGTCCAACAGGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCTTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGATCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAAt                                                                                                                                                                                                                                                <  2:587232/277‑1 (MQ=255)
                                   taTGTTCTTTTGCGGGAGTCCAGCATGAAAGTGCCATTAACGTTTAACGACTAACGTCGTGCGGGGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGGTCGTGCGATGCCAGACAAAATTGCCGTGGTCGAGAAGCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTa                                                                                                                                                                                                                                             <  2:1008013/288‑1 (MQ=255)
                                                                aGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATc                                                                                                                                                                                                                <  2:137437/289‑1 (MQ=255)
                                                                    aCATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGt                                                                                                                                                                                                             >  2:40059/1‑288 (MQ=255)
                                                                    aCATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGt                                                                                                                                                                                                             >  2:794449/1‑288 (MQ=255)
                                                                    aCATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGt                                                                                                                                                                                                             >  1:531488/1‑288 (MQ=255)
                                                                               tAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGt                                                                                                                                                                                                  >  2:1984757/1‑288 (MQ=255)
                                                                               tAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGt                                                                                                                                                                                                  >  1:231032/1‑288 (MQ=255)
                                                                               tAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTg                                                                                                                                                                                                 <  2:1493777/289‑1 (MQ=255)
                                                                                     acaacGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTg                                                                                                                                                                                           <  1:604059/289‑1 (MQ=255)
                                                                                      caacGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGt                                                                                                                                                                                          >  1:298666/1‑289 (MQ=255)
                                                                                            cgtGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCt                                                                                                                                                                                     >  1:449923/1‑288 (MQ=255)
                                                                                                   cgTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGGTTCCGTGCCGCTGTTGCCTTCCTGGCg                                                                                                                                                                             >  1:1086913/1‑289 (MQ=255)
                                                                                                    gTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCCGGCg                                                                                                                                                                             <  1:1868708/288‑1 (MQ=255)
                                                                                                     tATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTtgcc                                                                                                                                                                                                                            <  1:1491465/240‑1 (MQ=255)
                                                                                                      aTCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCggg                                                                                                                                                                           <  1:1761823/288‑1 (MQ=255)
                                                                                                      aTCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGa                                                                                                                                                                          <  2:1774942/289‑1 (MQ=255)
                                                                                                        cggaggaaaGGGTTATGGGTGGATGCTTCGCTGTGCGATTACTGGCAGCAGACAGATCGTGAGAAGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAg                                                                                                                                                                        <  2:636771/282‑1 (MQ=255)
                                                                                                          tCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCg                                                                                                                                                                             >  1:377396/1‑282 (MQ=255)
                                                                                                          tCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCg                                                                                                                                                                             <  2:377396/282‑1 (MQ=255)
                                                                                                              cAAGGTTTATGGGGGCATGCTTCGCTGGCCGATTACTGGCAGCAGACCTCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGaa                                                                                                                                                                   <  2:365740/288‑1 (MQ=255)
                                                                                                               aaGGGTTATGGGGCGATGCTTCGCTGGCCGAGTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAAc                                                                                                                                                                  <  2:531488/288‑1 (MQ=255)
                                                                                                                   gTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGgtg                                                                                                                                                             >  2:408197/1‑289 (MQ=255)
                                                                                                                       tGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGaa                                                                                                                                                                   <  1:1244779/279‑1 (MQ=255)
                                                                                                                       tGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTggg                                                                                                                                                         <  2:9544/289‑1 (MQ=255)
                                                                                                                          ggccatgcttcgcTGGCCGATGACTGGCAGCAGACCGCTTGTGCGATGACAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCGGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTg                                                                                                                                                       <  1:416349/276‑1 (MQ=255)
                                                                                                                          ggCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTg                                                                                                                                                       <  2:1135893/288‑1 (MQ=255)
                                                                                                                              atgcttcgcTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGa                                                                                                                                                                    <  1:557724/263‑1 (MQ=255)
                                                                                                                              atgcttcgcTGGACGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCaa                                                                                                                                                  >  1:1040481/9‑289 (MQ=255)
                                                                                                                               tgcttcgcTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAAt                                                                                                                                                                                                                                                <  1:239950/187‑1 (MQ=255)
                                                                                                                               tgcttcgcTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTCCAACTGCCTGGCTGGTGTGAAt                                                                                                                                                                                                                                                >  2:239950/8‑194 (MQ=255)
                                                                                                                                         gCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAg                                                                                                                                        >  2:1236251/1‑288 (MQ=255)
                                                                                                                                          ccGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAgg                                                                                                                                       <  2:222386/288‑1 (MQ=255)
                                                                                                                                                ccTGGCAGCAGACCTGTCGGGCGACTCCAGACTAAATTGCCGTGGTCGTTAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGGCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGTATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAAt                                                                                                                                <  2:662853/288‑1 (MQ=255)
                                                                                                                                                aCTGGCAGCAAACCGCTCGTGGGAAGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAAt                                                                                                                                <  2:1868990/289‑1 (MQ=255)
                                                                                                                                                   ggCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGtt                                                                                                                             >  2:216278/1‑289 (MQ=255)
                                                                                                                                                        cagACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTg                                                                                                                                                       <  1:872961/258‑1 (MQ=255)
                                                                                                                                                        cagACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCttt                                                                                                                         >  1:992871/1‑288 (MQ=255)
                                                                                                                                                                tCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGAc                                                                                                                 <  2:30292/288‑1 (MQ=255)
                                                                                                                                                                tCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACg                                                                                                                <  1:1339368/289‑1 (MQ=255)
                                                                                                                                                                                      gCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCgg                                                                                          <  1:40059/289‑1 (MQ=255)
                                                                                                                                                                                         gTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAg                                                                                       >  2:1499178/1‑289 (MQ=255)
                                                                                                                                                                                                      aTGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTg                                                                                                                                                       >  1:1425365/1‑212 (MQ=255)
                                                                                                                                                                                                      aTGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTg                                                                                                                                                       <  2:1425365/212‑1 (MQ=255)
                                                                                                                                                                                                       tGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGcc                                                                          >  1:1511927/1‑288 (MQ=255)
                                                                                                                                                                                                       tGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGcc                                                                          >  1:283876/1‑288 (MQ=255)
                                                                                                                                                                                                           gcatcgtACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGACAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGCAAGCAGCACCGGTGTGGGTGCTCAATAAGTGGCATGCAAAAATGTTCTTTGCACGGACGTTGTTTAAACAAACGCGTCCGGTAGATTTTATCCTTCCGATGc                                                                     >  2:1859146/7‑289 (MQ=255)
                                                                                                                                                                                                            catcgtACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAAt                                                                                                                                                 >  1:1116522/6‑213 (MQ=255)
                                                                                                                                                                                                             atcgtACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCaa                                                                   >  1:1200304/5‑289 (MQ=255)
                                                                                                                                                                                                              tcgtACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATTAGTGTCAGGCAAAAATGTTCGTTGCACCCACGTTGGTTAAACAAACGCGTCCGGTAGAATTAATCCTGCCGCTGCaaa                                                                  >  2:1187421/4‑289 (MQ=255)
                                                                                                                                                                                                              tcgtACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACCAACGCTGCCGGTAGATTTAATCCTGCCGCTGCaaa                                                                  >  2:1817441/4‑289 (MQ=255)
                                                                                                                                                                                                              tcgtACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCaaa                                                                  >  1:1957918/4‑289 (MQ=255)
                                                                                                                                                                                                                  acacCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTTCCCACGCAAAATcc                                                              >  2:1560637/1‑288 (MQ=255)
                                                                                                                                                                                                                  acacCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATc                                                               <  1:202504/288‑1 (MQ=255)
                                                                                                                                                                                                                    acCTAGAGCGCGCTCGATCACGCCGCGAGCTGTCTGGAAAACTGGATGTTAGCTAA‑GGGTATTGAATCAGGCGATCGCATCGCATGTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGc                                                            <  2:1095829/289‑1 (MQ=255)
                                                                                                                                                                                                                      cTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTggg                                                                                                                                                         <  1:741464/194‑1 (MQ=255)
                                                                                                                                                                                                                        ataGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTcc                                                        >  1:1095829/1‑289 (MQ=255)
                                                                                                                                                                                                                         taGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCCCCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCCCTGCAAAATCAACCTcc                                                        >  2:16967/1‑288 (MQ=255)
                                                                                                                                                                                                                           gcgcgcTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCcaca                                                     >  1:322895/1‑289 (MQ=255)
                                                                                                                                                                                                                           gcgcgcTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCcaca                                                     >  2:1311926/1‑289 (MQ=255)
                                                                                                                                                                                                                              cgcTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCg                                                                                                                                                                             >  2:817565/1‑166 (MQ=255)
                                                                                                                                                                                                                              cgcTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCg                                                                                                                                                                             <  1:817565/166‑1 (MQ=255)
                                                                                                                                                                                                                              cgcTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAAc                                                                                                                                                                  >  2:849564/1‑177 (MQ=255)
                                                                                                                                                                                                                              cgcTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACt                                                  >  1:1450256/1‑289 (MQ=255)
                                                                                                                                                                                                                               ggtcggtaaCGCCGCGAGCTGTATGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACt                                                  <  2:298666/280‑1 (MQ=255)
                                                                                                                                                                                                                                cTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATc                                                                                                                                                                                                                <  2:762184/129‑1 (MQ=255)
                                                                                                                                                                                                                                cTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATc                                                                                                                                                                                                                >  1:762184/1‑129 (MQ=255)
                                                                                                                                                                                                                                cTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGCAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGACTTAATCCTGCCGCTGAACAATGAGCTTACACAACGac                                                >  1:635326/1‑289 (MQ=255)
                                                                                                                                                                                                                                cTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTac                                                >  2:1898883/1‑289 (MQ=255)
                                                                                                                                                                                                                                cTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTac                                                >  1:83220/1‑289 (MQ=255)
                                                                                                                                                                                                                                cTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTac                                                >  1:1005843/1‑289 (MQ=255)
                                                                                                                                                                                                                                cTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTa                                                 >  2:446999/1‑288 (MQ=255)
                                                                                                                                                                                                                                cTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGAGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTac                                                >  1:1550793/1‑289 (MQ=255)
                                                                                                                                                                                                                                               aGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGct                                 >  2:1624908/1‑288 (MQ=255)
                                                                                                                                                                                                                                               aGCTGTCTGGCAAACTGGATGTTAGCCAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCAc                                                                                                                     >  1:1807602/1‑205 (MQ=255)
                                                                                                                                                                                                                                                              tGGATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTc                  <  2:322895/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                gATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGa                                                                                                                                                                          >  2:1034573/1‑135 (MQ=255)
                                                                                                                                                                                                                                                                gATGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGa                                                                                                                                                                          <  1:1034573/135‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tGTTAGCGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCg               >  2:459055/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                        cGAA‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGc              >  1:1283331/1‑283 (MQ=255)
                                                                                                                                                                                                                                                                          aa‑GGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCgg                                                                                          >  2:716380/1‑205 (MQ=255)
                                                                                                                                                                                                                                                                               gTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGATTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCACCTCTTccc   >  1:580189/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                tATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCACCTCTTCCCt  <  1:917335/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                      
TAATCATGCATCCCACAGGCCCGCATCTCGGGCCTGATGTTCTGTTTCGAGAGTCCAACATGAAAGTGACATTAACGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTAGCCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCAAGCTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAA‑GGGTATTGAAAGTGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGCCGCTGTTGCCTTCCTGGCGGGAAGCAGAACTGGTGTGGGTGCTCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCACCTCTTCCCT  >  CP042583/1464923‑1465481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A