Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,944,975 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,944,9750AT99.6% 577.6 / NA 245intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (135/109);  minor base G (0/1);  total (135/110)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,944,9760CG99.6% 566.3 / NA 245intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (134/109);  minor base A (0/1);  total (135/110)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,944,9770TA100.0% 582.9 / NA 245intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (135/110);  total (135/110)

Only 100 of 245 total aligned reads displayed.

ATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCAGCTTTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGACTAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGG  >  CP042583/1944695‑1945255
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                      
aTCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAAtttt                                                                                                                                                                                                                                                                                  >  1:1671782/1‑289 (MQ=255)
     ccACTTTCCCGGCGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGCGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATAc                                                                                                                                                                                                                                                                             <  1:1894270/289‑1 (MQ=255)
                           ctTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCcggcgg                                                                                                                                                                                                                                                       <  1:1818867/289‑1 (MQ=255)
                                cTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCagag                                                                                                                                                                                                                                                  >  1:1750046/1‑289 (MQ=255)
                                    ggCGATCATTTTTATCGCGCCGCCAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTATCATCAATTTACAGATTACCAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCTGCGGCAGAGTTa                                                                                                                                                                                                                                               >  1:1395516/1‑288 (MQ=255)
                                      cGATCATTTTTATCGCGCCGCCAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCACATTTAGcc                                                                                                                                                                                                                                            >  2:1977016/1‑289 (MQ=255)
                                                tATCGCGCCGCCAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCgg                                                                                                                                                                                                                                  <  1:416867/289‑1 (MQ=255)
                                                       ccgccAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTACGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGGGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGGTTTTCCGCTCCCGCGGCAGATTTAGCCAGCGCCACGGGTGTTGa                                                                                                                                                                                                                           >  2:1188564/1‑289 (MQ=255)
                                                       ccgccAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGc                                                                                                                                                                                                                                             >  2:1614090/1‑271 (MQ=255)
                                                       ccgccAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGc                                                                                                                                                                                                                                             <  1:1614090/271‑1 (MQ=255)
                                                               aTCGCAGATCATAAGTACATTTATGTTATCTTGTTTATTGCAGCCCGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACgg                                                                                                                                                                                                                     <  2:1486360/287‑1 (MQ=255)
                                                               aTCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACgg                                                                                                                                                                                                                     <  2:1427618/287‑1 (MQ=255)
                                                                    caagcttaatgataTGTCTGTTTCATTGTGTATTGAAGCCATATTTTTTGACACGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTACCATCAATTTACAGATTAACAGATAAACTCTTTATAAACGGCAATGGAATGGCAGAAATGTGAAGTTAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCt                                                                                                                                                                                                              <  2:1069047/276‑1 (MQ=255)
                                                                       gCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCt                                                                                                                                                                                                              <  2:621082/286‑1 (MQ=255)
                                                                       gCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACAATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCtt                                                                                                                                                                                                             <  1:1353930/287‑1 (MQ=255)
                                                                        cATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGc                                                                                                                                                                                                                                             <  1:1605036/254‑1 (MQ=255)
                                                                         aTAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCt                                                                                                                                                                                                         <  2:1170356/289‑1 (MQ=255)
                                                                           aaGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCttt                                                                                                                                                                                                       >  1:543886/1‑289 (MQ=255)
                                                                             gTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTc                                                                                                                                                                                                      >  1:1096999/1‑288 (MQ=255)
                                                                             gTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTc                                                                                                                                                                                                      >  2:1810827/1‑288 (MQ=255)
                                                                                cATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGTGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCCCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCAcc                                                                                                                                                                                                   >  2:932362/1‑288 (MQ=255)
                                                                                 aTGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCa                                                                                                                                                                                                 <  1:1490505/289‑1 (MQ=255)
                                                                                  tGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCAt                                                                                                                                                                                                >  2:238626/1‑289 (MQ=255)
                                                                                  tGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCAt                                                                                                                                                                                                >  1:581954/1‑289 (MQ=255)
                                                                                      tGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCt                                                                                                                                                                                                              <  1:1306289/271‑1 (MQ=255)
                                                                                      tGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCt                                                                                                                                                                                                              >  2:1306289/1‑271 (MQ=255)
                                                                                      tGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCt                                                                                                                                                                                                              <  1:1935661/271‑1 (MQ=255)
                                                                                        ttATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTa                                                                                                                                                                                          >  2:1128749/1‑289 (MQ=255)
                                                                                        ttATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTa                                                                                                                                                                                          >  2:618910/1‑289 (MQ=255)
                                                                                                tttATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGAt                                                                                                                                                                                      <  2:781619/285‑1 (MQ=255)
                                                                                                 ttATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACg                                                                                                                                                                                 <  1:971953/289‑1 (MQ=255)
                                                                                                 ttATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACg                                                                                                                                                                                 <  1:1264976/289‑1 (MQ=255)
                                                                                                  tATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACgg                                                                                                                                                                                                                     >  2:1726861/1‑252 (MQ=255)
                                                                                                  tATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACgg                                                                                                                                                                                                                     <  1:1726861/252‑1 (MQ=255)
                                                                                                     tGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTACGATCAACGctc                                                                                                                                                                              >  1:466392/1‑288 (MQ=255)
                                                                                                     tGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGctct                                                                                                                                                                             <  2:543886/289‑1 (MQ=255)
                                                                                                          cacgattttttGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCa                                                                                                                                                                                                 >  1:812076/6‑264 (MQ=255)
                                                                                                          cacgattttttGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCa                                                                                                                                                                                                 <  2:812076/259‑1 (MQ=255)
                                                                                                          cacgattttttGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTgg                                                                                                                                                                        >  1:871508/6‑289 (MQ=255)
                                                                                                             gattttttGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCa                                                                                                                                                                     <  1:1128749/287‑1 (MQ=255)
                                                                                                             gattttttGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCa                                                                                                                                                                     <  1:1226801/287‑1 (MQ=255)
                                                                                                                 ttttGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACt                                                                                                                                                                 <  1:1281559/289‑1 (MQ=255)
                                                                                                                   ttGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTcc                                                                                                                                                               <  2:871508/289‑1 (MQ=255)
                                                                                                                              ttccattGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCAc                                                                                                                                                    <  2:1103125/289‑1 (MQ=255)
                                                                                                                                    tGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATGAACAGATAAACTCTTTATCAACGACAATGCAATGGCAGAAATGAGAAGTGAAACGGAGGGTTACTTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTGAATACCGCCGTTTTTCAGCGCCGGCGGCAGATTTAGCAAGCGCACCGGTTGTTGAAAACGTGCCAGCTTATCTTTCACCCATCCACTAAGATCAACGCTCTCGTGGTCATCCTCCATCACCGCAACCGGTCg                                                                                                                                              >  2:1753740/1‑289 (MQ=255)
                                                                                                                                     gCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGa                                                                                                                                             >  1:1223343/1‑289 (MQ=255)
                                                                                                                                     gCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGa                                                                                                                                             <  1:1902985/289‑1 (MQ=255)
                                                                                                                                           tCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACg                                                                                                                                                                                 <  2:102453/247‑1 (MQ=255)
                                                                                                                                           tCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACt                                                                                                                                                                 <  1:1100005/263‑1 (MQ=255)
                                                                                                                                           tCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCa                                                                                                                                       >  2:751988/1‑289 (MQ=255)
                                                                                                                                              tGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCA‑CCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAAc                                                                                                                                    >  1:893029/1‑288 (MQ=255)
                                                                                                                                                tttGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTTTTGAAAACGGGCCAGCTTATCTTTCACCCAt                                                                                                                                                                                                <  2:1579970/227‑1 (MQ=255)
                                                                                                                                                tttGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCAc                                                                                                                                                    >  2:165244/1‑271 (MQ=255)
                                                                                                                                                tttGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACt                                                                                                                                   >  1:32393/1‑288 (MQ=255)
                                                                                                                                                tttGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACt                                                                                                                                   >  2:1359279/1‑288 (MQ=255)
                                                                                                                                                tttGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACt                                                                                                                                   >  1:1535260/1‑288 (MQ=255)
                                                                                                                                                tttGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACt                                                                                                                                   >  1:1326093/1‑288 (MQ=255)
                                                                                                                                                tttGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTGTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACCCCATCACCGCCCACGGTCGATGACCAAACt                                                                                                                                   >  1:257331/1‑288 (MQ=255)
                                                                                                                                                  tGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACAGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCAc                                                                                                                                                                                                                                     >  2:1274200/1‑188 (MQ=255)
                                                                                                                                                     tAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTg                                                                                                                              >  1:1024757/1‑288 (MQ=255)
                                                                                                                                                         agTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATCCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGCCGCTGACCAAACTCCTTGTCgac                                                                                                                         >  2:208665/1‑287 (MQ=255)
                                                                                                                                                              tCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACgg                                                                                                                    >  1:1205222/1‑289 (MQ=255)
                                                                                                                                                                   tCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACg                                                                                                               >  2:1749141/1‑289 (MQ=255)
                                                                                                                                                                                tAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCa                                                                                                  >  1:846635/1‑289 (MQ=255)
                                                                                                                                                                                 aaCAGATAAACTCTTTATCAACGGCAATGGATTGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCaa                                                                                                 >  1:549784/1‑289 (MQ=255)
                                                                                                                                                                                 aaCAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTACTACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACgggg                                                                                                                  <  2:1878926/272‑1 (MQ=255)
                                                                                                                                                                                      aTAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCa                                                                                                  >  1:625533/1‑283 (MQ=255)
                                                                                                                                                                                      aTAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAAcc                                                                                             >  1:1556143/1‑288 (MQ=255)
                                                                                                                                                                                       tacaCTCTTTATCAACGGCAATGGAATGGCAGAAATGGAGAGTGAAACGGAGGGGTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGc                                                                                           <  2:1535260/286‑1 (MQ=255)
                                                                                                                                                                                        aaaCTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCg                                                                                                                                                                           <  1:1689629/208‑1 (MQ=255)
                                                                                                                                                                                        aaaCTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACGCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCa                                                                                          >  2:565505/1‑289 (MQ=255)
                                                                                                                                                                                          aatctTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAgg                                                                                        <  2:275501/287‑1 (MQ=255)
                                                                                                                                                                                          aCTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGa                                                                                                                 <  1:1990729/264‑1 (MQ=255)
                                                                                                                                                                                          aCTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAgg                                                                                        <  2:1288714/289‑1 (MQ=255)
                                                                                                                                                                                          aCTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAgg                                                                                        >  2:695447/1‑289 (MQ=255)
                                                                                                                                                                                          aCTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAgg                                                                                        <  2:518749/289‑1 (MQ=255)
                                                                                                                                                                                             ctTTATTAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGATGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGAtg                                                                                     <  2:1006492/289‑1 (MQ=255)
                                                                                                                                                                                              tttATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGAtgt                                                                                    >  1:1401963/1‑289 (MQ=255)
                                                                                                                                                                                                      cGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAAt                                                                            >  1:300386/1‑289 (MQ=255)
                                                                                                                                                                                                      cGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAAt                                                                            >  1:767286/1‑289 (MQ=255)
                                                                                                                                                                                                        gCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAAGATGTGCCGCACtt                                                                           >  2:1934916/1‑288 (MQ=255)
                                                                                                                                                                                                         cAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTa                                                                          <  2:846635/288‑1 (MQ=255)
                                                                                                                                                                                                          aatggaatggCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTAcg                                                                        <  2:145529/289‑1 (MQ=255)
                                                                                                                                                                                                            tggaatggCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTgcag                                                                                <  1:1011504/279‑1 (MQ=255)
                                                                                                                                                                                                                 tggCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGCTGCACACACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGATcc                                                                  >  2:637680/1‑287 (MQ=255)
                                                                                                                                                                                                                   gCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGTATGTGCAGCAATTACGCGCTCGAct                                                               >  1:1058269/1‑289 (MQ=255)
                                                                                                                                                                                                                        aaTGAGTAGGGAAACGTAGGGTTAATTATTGCGGACGTTGCACTCACGTTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCCTTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGActtct                                                            <  2:1058269/287‑1 (MQ=255)
                                                                                                                                                                                                                        aaTGAGAAGTTAAAAGGAGGGTTAATTATTGCTGTCGTTGCACCCACTATTTTAACGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACttcttc                                                          <  2:1910044/289‑1 (MQ=255)
                                                                                                                                                                                                                          tGAGAGTTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCg                                                        <  1:637680/289‑1 (MQ=255)
                                                                                                                                                                                                                                    aaCGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATAcc                                              <  2:1326093/289‑1 (MQ=255)
                                                                                                                                                                                                                                          gggTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCTGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCtctc                                         <  1:751988/288‑1 (MQ=255)
                                                                                                                                                                                                                                              tAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCTCCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCa                                    <  1:1424528/289‑1 (MQ=255)
                                                                                                                                                                                                                                                attattGCTGACGTTGCACCCACTCGTTGTGCGCCTAACGGGAAATTTTAATGCCGCCGTTTTTCGGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGACAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTTGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAAt                                                 <  2:1593548/274‑1 (MQ=255)
                                                                                                                                                                                                                                                          cccgtGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAAGTCCGCCGTTTTTCCGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGa                        <  2:4039/285‑1 (MQ=255)
                                                                                                                                                                                                                                                           cGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGa                        <  1:1149007/288‑1 (MQ=255)
                                                                                                                                                                                                                                                           cGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGa                        <  2:1713411/288‑1 (MQ=255)
                                                                                                                                                                                                                                                            gTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCCGAATACCCCTCCCGCCACTGAAGAATAAAtt                      >  2:1417796/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                      ctctTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGa                                                                                                                                             <  1:118654/160‑1 (MQ=255)
                                                                                                                                                                                                                                                                             aGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACaa     >  1:220137/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                 ccTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTcc                                                         <  1:1430491/233‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                      
ATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAGAGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCAGCTTTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGACTAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGG  >  CP042583/1944695‑1945255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A