Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 592,656 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583592,6560GC99.2% 611.3 / NA 258intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (113/142);  minor base T (1/1);  total (114/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP042583592,6570CG99.6% 607.2 / NA 258intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (112/144);  total (114/144)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583592,6580TA99.6% 612.2 / NA 258intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (113/144);  minor base G (1/0);  total (114/144)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 258 total aligned reads displayed.

CGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGGCTTTCGCAATTCTCAAAGACACCGCTA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGGCTTAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTACTCATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTC  >  CP042583/592376‑592934
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                     
          cagATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATa                                                                                                                                                                                                                                                                       >  2:1529690/1‑288 (MQ=255)
            cgtCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATActc                                                                                                                                                                                                                                                                    <  2:975509/287‑1 (MQ=255)
              tCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATAct                                                                                                                                                                                                                                                                     <  2:1208498/286‑1 (MQ=255)
                gCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCg                                                                                                                                                                                                                                                                >  2:684471/1‑289 (MQ=255)
                      cGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTaca                                                                                                                                                                                                                                                          <  1:2362476/289‑1 (MQ=255)
                       gCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTacac                                                                                                                                                                                                                                                         <  1:1788305/289‑1 (MQ=255)
                            gtaggCGGTTCACAAGTCGGCACGGAACAGGGACTCAACAAAGTATTCCACAAACTGATTCCCCGCCGGATGGAGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCt                                                                                                                                                                                                                                                    <  2:1645475/286‑1 (MQ=255)
                            gTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCt                                                                                                                                                                                                                                                    >  1:1726737/1‑289 (MQ=255)
                               ggCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGa                                                                                                                                                                                                                                                 >  1:808357/1‑289 (MQ=255)
                                gCGTTGAACAAGTCGGCAAGGAAAAGCGCCTCACCAAAGTATTCCACAAACTGATTCACAGCTGGATGGCGACGGAAAAATTCGCCGCACTGATGACCAGGGCAATCAAACGCGCAAATACCTTGACTGACATAATGGATCAGCCCGCGGCAGAAAGGCGGGCGACGGTGGGGTTCATAATGGTTGGATGCGGTGACTCATTGGTGATCCTTAAGTTCGCGTTGCGATTCCGTATTCTCCAAGGCACACGAA‑ATACGGGACAAACACCCTGGAGATACACGCTCTTgtg                                                                                                                                                                                                                                                >  2:2665260/1‑287 (MQ=255)
                                    tGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAGGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGAtttt                                                                                                                                                                                                                                             <  1:2280660/288‑1 (MQ=255)
                                    tGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTc                                                                                                                                                                                                                                            <  2:765206/289‑1 (MQ=255)
                                      cacaAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAg                                                                                                                                                                                                                                          >  1:1473507/1‑289 (MQ=255)
                                      caacactCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAg                                                                                                                                                                                                                                          <  2:2296474/283‑1 (MQ=255)
                                         aaGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAg                                                                                                                                                                                                                                       <  1:2520028/289‑1 (MQ=255)
                                         aaGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAg                                                                                                                                                                                                                                       <  1:23054/289‑1 (MQ=255)
                                           gTCGGCACGGAACAGCGCCTCTCCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCCACGCCCTGATGGCCAGGGCAATCAAAGGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGCAGTAAGGAGGGCCGAGTTTAGTGTCATAATGGCAGGTTGCGGTATCTTACTTGCGACCATTCAATTTTAGTCTCGATGCGTTATTCTCAACGA‑ACCCCGACATCGTGGGCAAAGACTTGGCGGTTACCCGTTCTGGTTTTTCAGTAGCa                                                                                                                                                                                                                                     >  2:250107/1‑289 (MQ=255)
                                           gTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGCCGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGAACATAAAATTCTCGTCGCCATTCGCAATTCTCAAAGACACCCGAA‑ATACGGCGCAAATACTCTGCGGGTACACGTTATTGATTTCTAGTAGCa                                                                                                                                                                                                                                     >  2:234502/1‑289 (MQ=255)
                                           gTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCaaa                                                                                                                                                                                                                                                                         <  1:2428046/253‑1 (MQ=255)
                                           gTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCaaa                                                                                                                                                                                                                                                                         >  2:2428046/1‑253 (MQ=255)
                                           gTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTa                                                                                                                                                                                                                                        <  1:742302/286‑1 (MQ=255)
                                             cGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCaat                                                                                                                                                                                                                                   <  2:1907945/289‑1 (MQ=255)
                                             cGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                                                                                                                                                                                     <  1:2606500/287‑1 (MQ=255)
                                                cACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTAAACGTTCTTGATTTTCAGTAGCaataaa                                                                                                                                                                                                                                >  1:1955962/1‑289 (MQ=255)
                                                   ggATCAACGCCTCACCAAAGTATTCCACAAACTGATTCCCTGCCTGATGGCGACGGCAATATTCGCCGCCCTGATGACCAGGGCAATCGAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAATAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTAGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCATTAGCAATAAATAc                                                                                                                                                                                                                             <  2:1955962/289‑1 (MQ=255)
                                                     aaCAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCg                                                                                                                                                                                                                           >  1:2632049/1‑289 (MQ=255)
                                                     aaCAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTAACTGTTCTTGATTTTCAGTAGCAATCAATAcct                                                                                                                                                                                                                           >  2:742302/1‑288 (MQ=255)
                                                     aaCAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTAAACGTTCTTGATTTTCAGTAGCAATAAAAACCg                                                                                                                                                                                                                           >  2:1181188/1‑289 (MQ=255)
                                                     aaCAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACGCTGCGGGTACACGTTCTTGATTTTCAGTa                                                                                                                                                                                                                                        <  1:2716942/276‑1 (MQ=255)
                                                     aaCAGCGCCTACCCAAAGTATTCCACAAAATGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTa                                                                                                                                                                                                                                        <  2:267974/276‑1 (MQ=255)
                                                          cgcCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTTGCAATAAATACCGAGAt                                                                                                                                                                                                                       >  1:1365867/1‑288 (MQ=255)
                                                           gcCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCggg                                                                                                                                                                                                                         <  2:2380341/285‑1 (MQ=255)
                                                           gcCTCACCAAAGTAGTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCACTCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCggg                                                                                                                                                                                                                         <  2:2420796/285‑1 (MQ=255)
                                                               cACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCaataaata                                                                                                                                                                                                                              >  1:267974/1‑276 (MQ=255)
                                                               cACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATa                                                                                                                                                                                                                 >  1:78488/1‑289 (MQ=255)
                                                               cACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATa                                                                                                                                                                                                                 >  1:304595/1‑289 (MQ=255)
                                                               cACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGATGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGCGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATCAAATTCTCGTCGCGATTCGAAATTCTCAAAGACCCCCGAA‑ATACGGGGCAAATACTCTGCGGGTAACCGTTCTTGATTTTCAGTAGAAATAAATACCAGGATGCAATa                                                                                                                                                                                                                 >  2:1327396/1‑289 (MQ=255)
                                                                       tATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATAGGGGGCAAATACTCTGCGGGTACACGTTCATGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTgg                                                                                                                                                                                                         >  2:1028749/1‑289 (MQ=255)
                                                                        aTTCCCCAACCTGAGTCGCCGCCGCATGGCGACGGAAAAAGTGGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTg                                                                                                                                                                                                          <  2:451339/287‑1 (MQ=255)
                                                                          tCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCaata                                                                                                                                                                                                                                  <  2:2045448/261‑1 (MQ=255)
                                                                          tCCACAAACTAATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCTCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCa                                                                                                                                                                                                                                           >  2:1594819/1‑252 (MQ=255)
                                                                          tCCACAAACTAATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCTCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCa                                                                                                                                                                                                                                           <  1:1594819/252‑1 (MQ=255)
                                                                                 aCTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCATGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGAttta                                                                                                                                                                                               >  2:369146/1‑288 (MQ=255)
                                                                                 aCTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAACGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGTATGCCATAACCCGTGGCATGTATTTc                                                                                                                                                                                               >  2:1069550/1‑289 (MQ=255)
                                                                                     aTTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAC‑ATACGTGGCAATAACTCTGCGGGTACACGCTCTTGATTTTGAGTATCAAAAAATACCGGGATGCCATAACCGCTGCCTTCGTTTTCaac                                                                                                                                                                                            >  2:2228217/1‑286 (MQ=255)
                                                                                     aTTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCg                                                                                                                                                                                           >  2:2715123/1‑289 (MQ=255)
                                                                                      ttCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGa                                                                                                                                                                                          <  1:1229153/289‑1 (MQ=255)
                                                                                      ttCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGa                                                                                                                                                                                          >  1:2424877/1‑289 (MQ=255)
                                                                                           cgccgGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGttt                                                                                                                                                                                      >  1:696016/1‑288 (MQ=255)
                                                                                           cgccgGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGttt                                                                                                                                                                                      >  1:2068592/1‑288 (MQ=255)
                                                                                              cgGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTcc                                                                                                                                                                                   <  2:2555264/288‑1 (MQ=255)
                                                                                                               aTTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAg                                                                                                                                                                 <  1:1435590/289‑1 (MQ=255)
                                                                                                                                 cAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTgccg                                                                                                                                                >  1:996262/1‑288 (MQ=255)
                                                                                                                                    ggCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAg                                                                                                                                                                                                                                          <  2:1261575/195‑1 (MQ=255)
                                                                                                                                    ggCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTa                                                                                                                                                                            <  1:1523783/257‑1 (MQ=255)
                                                                                                                                    ggCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTa                                                                                                                                                                            >  2:1523783/1‑257 (MQ=255)
                                                                                                                                        aTCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCg                                                                                                                                        <  1:1529690/289‑1 (MQ=255)
                                                                                                                                         tCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGGATGTAACATCGTTGATCGCAAAAACTATTCACGCcaaccatc                                                                                                                                       >  2:38867/1‑283 (MQ=255)
                                                                                                                                                   tGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAACATTATTTCCGCCACATCGATATTACGACt                                                                                                                             >  2:1735708/1‑289 (MQ=255)
                                                                                                                                                   tGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACt                                                                                                                             <  1:1058634/289‑1 (MQ=255)
                                                                                                                                                       aCCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCa                                                                                                                         <  2:2627569/289‑1 (MQ=255)
                                                                                                                                                       aCCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCa                                                                                                                         <  2:2068592/289‑1 (MQ=255)
                                                                                                                                                         cTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCAtt                                                                                                                       <  1:2127808/289‑1 (MQ=255)
                                                                                                                                                          ttgattgaCATAATCGACCATTGCGCGGAAGAAAAGTGGGCGCAGTTGAGTTTCATAGTGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCTATTCTCCAAGACACCCGAA‑ATACGGGTCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTa                                                                                                                      <  1:1327396/289‑1 (MQ=255)
                                                                                                                                                          ttgattgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTa                                                                                                                      <  2:1726737/289‑1 (MQ=255)
                                                                                                                                                           tgattgaCATAATCGACCATTGCGCGGAAGAAAGGTTGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTaa                                                                                                                     <  1:28841/289‑1 (MQ=255)
                                                                                                                                                           tgattgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTaa                                                                                                                     >  1:238651/1‑289 (MQ=255)
                                                                                                                                                            gattgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAAt                                                                                                                    <  2:78488/289‑1 (MQ=255)
                                                                                                                                                             attgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTg                                                                                                                                                                                                                                                                  <  2:2694392/146‑1 (MQ=255)
                                                                                                                                                             attgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGc                                                                                                                                                                                                                                      >  2:1484941/1‑174 (MQ=255)
                                                                                                                                                             attgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGc                                                                                                                                                                                                                                      <  1:1484941/174‑1 (MQ=255)
                                                                                                                                                             attgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTACCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTATTg                                                                                                                   >  1:2064179/1‑289 (MQ=255)
                                                                                                                                                               tgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGATTTTACTTAAttga                                                                                                                 >  2:876254/1‑286 (MQ=255)
                                                                                                                                                               tgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTa                                                                                                                 >  1:2466227/1‑289 (MQ=255)
                                                                                                                                                               tgaCATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTa                                                                                                                 >  1:1002462/1‑289 (MQ=255)
                                                                                                                                                                  cATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGa                                                                                                               <  2:12771/288‑1 (MQ=255)
                                                                                                                                                                     aaTCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACgggg                                                                                                                                                                                                                                                                             <  1:175208/127‑1 (MQ=255)
                                                                                                                                                                      aTCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTAt                                                                                                                                                     >  2:1463155/1‑246 (MQ=255)
                                                                                                                                                                       tCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACAtt                                                                                                         >  2:647349/1‑289 (MQ=255)
                                                                                                                                                                           acagggacgaggagaaAGGGGGGCGCAGTTGAGTTTCACAACGGCTGGCAACGAGTTCAACCTGGCGACCATAAAATTCTCGTGCCAATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTATCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCg                                                                                                     <  2:2527646/277‑1 (MQ=255)
                                                                                                                                                                               tGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGc                                                                                                 >  1:650697/1‑289 (MQ=255)
                                                                                                                                                                                gcgcGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTg                                                                                                  <  1:383652/287‑1 (MQ=255)
                                                                                                                                                                                gcgcGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTg                                                                                                  <  1:1835927/287‑1 (MQ=255)
                                                                                                                                                                                 cgcgGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTcc                                                                                                                                                                                   >  2:947894/1‑205 (MQ=255)
                                                                                                                                                                                         aaaGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCaaa                                                                                                                                                                                                                                                                         >  1:1577638/1‑111 (MQ=255)
                                                                                                                                                                                         aaaGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAAc                                                                                       >  1:605798/1‑289 (MQ=255)
                                                                                                                                                                                                ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTATCGCTGCGTGGTAAAACAGTCCgg                                                                                >  1:1989698/1‑289 (MQ=255)
                                                                                                                                                                                                                  aaTGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCTGGGCAAGAATCACTAACCGc                                                              <  1:1666856/289‑1 (MQ=255)
                                                                                                                                                                                                                      gctggcAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAAtt                                                          >  1:2452821/1‑289 (MQ=255)
                                                                                                                                                                                                                         ggcAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTg                                                                                                  >  1:1116320/1‑246 (MQ=255)
                                                                                                                                                                                                                                   tCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAAttttgaca                                             <  1:2552661/289‑6 (MQ=255)
                                                                                                                                                                                                                                            caaCCATAAAATTCTCGTCCCGATTCGAAATACTCAAAGACACCAGAA‑ATACGGGGCAAATACTCTGCGGGTAAACGTTCTTGATTTTCAGTAGCAATAAATACCGGTATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCAt                                     <  2:2170847/286‑1 (MQ=255)
                                                                                                                                                                                                                                              aCCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAAttt                                                  >  2:2735137/1‑273 (MQ=255)
                                                                                                                                                                                                                                               ccATAAAATTCTCGTTGAGATTCGCAATTCTCAAAGACAACCGAA‑CTACGGGGCAACTACTCTGCGGGTACACGTTCTTGATTGTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATctct                                 <  2:2609496/289‑1 (MQ=255)
                                                                                                                                                                                                                                               acATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATctct                                 <  2:1816667/288‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaTTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTAttt                           <  1:1773154/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gattCGCAATTCTCAAAGACACCCGAA‑ATACGGTGCAAATCCTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGc                <  1:465348/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ttCGCAATGATCAAAGCCAACCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAg              <  2:1765974/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                              cAAAGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTtc  <  2:640335/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               aaaGACACCCGAA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATctct                                 >  1:405293/1‑257 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                     
CGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGGCTTTCGCAATTCTCAAAGACACCGCTA‑ATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGGCTTAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTACTCATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTC  >  CP042583/592376‑592934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A