Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 598,446 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583598,4460GC99.4% 784.3 / NA 336intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base C (176/158);  total (176/160)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583598,4470CG99.1% 786.9 / NA 336intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base G (174/159);  total (176/160)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583598,4480TA99.7% 792.0 / NA 336intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (175/160);  minor base C (1/0);  total (176/160)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

CCACCACCAGCGGCGTGGCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGGCTCTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGGCTAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCGCTCGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCACTCAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTG  >  CP042583/598169‑598723
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                   
  acccccgGCGGCGTGGCACCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTtgtg                                                                                                                                                                                                                                                                          <  2:2219309/289‑1 (MQ=255)
   ccaccaGCGGCGTGGCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAAAACTGCGGCCTTCCGGCGTTTCATCATCCAGCGACGCCAGTtggta                                                                                                                                                                                                                                                                         >  2:1680261/1‑286 (MQ=255)
             cgTGGCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTcagc                                                                                                                                                                                                                                                                >  1:1603752/1‑288 (MQ=255)
                       tGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGtttttt                                                                                                                                                                                                                                                     <  1:608790/289‑1 (MQ=255)
                              cAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCAc                                                                                                                                                                                                                                              >  1:1290893/1‑289 (MQ=255)
                                 ccTGATCTGCTTTTTGATCAACATCGGAAGGGAAGGGACCACCGTGAGCCTCAACATGGCGACAGATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGcc                                                                                                                                                                                                                                           <  2:1110825/289‑1 (MQ=255)
                                  cTGAACGACTGTTTGATCAACATCGGTACGGAAGTGACCACCGTTGACCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACTGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGccc                                                                                                                                                                                                                                          <  2:1483162/289‑1 (MQ=255)
                                      tCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTgcg                                                                                                                                                                                                                                      <  1:69877/289‑1 (MQ=255)
                                      tCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAACGACTGCACATCGCG‑CTCGCGCAGGTGAAAACGCTGCTTGGCGAGGATACCAATACTGCCGCCTTCCGGCGTTTCCTCACACAACGAAGCCCTTTGAGCCGCGTACGCCAGCGTTGTGTCACCCCCCCCCTgc                                                                                                                                                                                                                                       >  2:2368627/1‑288 (MQ=255)
                                                     aCATCGGTAGGGAAGTGACCCCCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGATCAGCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCCGCGGTTCATCAGCCAGCGAAGCCAGTTGGGCGTCGTCAGCCACCGTTTTTTCATCCTCGCCCTCCGCCAGCGTAAActcc                                                                                                                                                                                                                        >  2:938728/1‑287 (MQ=255)
                                                     aCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAActccg                                                                                                                                                                                                                       <  1:1938736/289‑3 (MQ=255)
                                                     aCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAActccg                                                                                                                                                                                                                       >  1:2642672/1‑287 (MQ=255)
                                                            tAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAActccgacgcct                                                                                                                                                                                                                 <  1:1354010/288‑9 (MQ=255)
                                                                gAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACg                                                                                                                                                                                                             <  2:2510642/288‑1 (MQ=255)
                                                                   gTGACCTCCGTTAGCCTCAACTTGGGTACGTATGTCATCGACAGAACCTTGACGGATAATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGAAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTTGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTa                                                                                                                                                                                                         <  2:769430/289‑1 (MQ=255)
                                                                   gTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCCGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATATACTCCGCCGCCGCACGGtt                                                                                                                                                                                                          >  2:464613/1‑288 (MQ=255)
                                                                    tGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTtgtg                                                                                                                                                                                                                                                                          <  2:1424589/223‑1 (MQ=255)
                                                                    tGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTtgtg                                                                                                                                                                                                                                                                          >  1:1424589/1‑223 (MQ=255)
                                                                     gACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTa                                                                                                                                                                                                         <  1:1224863/287‑1 (MQ=255)
                                                                     gACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTAcc                                                                                                                                                                                                       <  1:2386073/289‑1 (MQ=255)
                                                                        caccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAg                                                                                                                                                                                                    <  2:689298/289‑1 (MQ=255)
                                                                           cGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGtttttt                                                                                                                                                                                                                                                     <  1:2240573/237‑1 (MQ=255)
                                                                                   tCAACAGGGCGCAGAATGGCATCGACAAAACCTTTTCGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGc                                                                                                                                                                                         <  2:2155202/289‑1 (MQ=255)
                                                                                    tgagagtggggggaAGGGCGTCGACGGAGCCTTGACGGGGCGGGCGGTTGTCGATGATGGTTCCGCTCATCCGGCTTTGCGCAGTAAACGGTAACAAGGTGGCATGGAGCGACTGCCCATCGCG‑CTCGCGCAGGTTAAAACGCTGCGTGGCGAGGATCACAATACGGAGGCCGTCCGGCGTTTCAGCAGCCAGCGAGGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCCTCCACGCCCTGCGCGGGGGTAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGc                                                                                                                                                                                         <  2:2064655/276‑1 (MQ=255)
                                                                                     aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGtttttt                                                                                                                                                                                                                                                     >  1:806614/1‑227 (MQ=255)
                                                                                     aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGtttttt                                                                                                                                                                                                                                                     <  2:806614/227‑1 (MQ=255)
                                                                                     aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCg                                                                                                                                                                                       >  1:2580591/1‑289 (MQ=255)
                                                                                     aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCg                                                                                                                                                                                       >  1:1097711/1‑289 (MQ=255)
                                                                                     aaCATGGCGACGAATGCCATCGACAGAACCTTTACGGATCATGCGGGTGTCGATGTTTATCCCCCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGTTTGTAGCGACTGCATATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAGTACTGCGGCCCTCCTGCGTTTCATCATCCAGCCAAGCCAGTAGTGCGGCCTCAGCCAGTGTCTTGTAATCCACGCCCTTCCAGAGTGTAAACTACCACGCCTGTCGGTTAACCAGTGGGATGGTTCCg                                                                                                                                                                                       >  2:345257/1‑289 (MQ=255)
                                                                                            cgacgaATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTa                                                                                                                                                                                 <  1:2032442/288‑1 (MQ=255)
                                                                                            cgacgaATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTa                                                                                                                                                                                 <  1:1680261/288‑1 (MQ=255)
                                                                                               cgaATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCAAAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCTAGTGTGATGGTGCCGGGTTTATc                                                                                                                                                                               >  1:2460986/1‑287 (MQ=255)
                                                                                                      taagaagcagaCCTTTAGGGTTCATGCGGTTGTCGATGTTGACCCCGCTCTTCCGGCTTTGCGCAGGAAACGGTACAAAGGTGGCATGCAGCGACTGCACATCGCG‑CTCGCGTAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCGGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAg                                                                                                                                                                      <  2:1836252/279‑1 (MQ=255)
                                                                                                                      aCGGATCAGGCGGTAGGCGATGTTGATCCCGCGCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCt                                                                                                                                                      <  2:2677810/289‑1 (MQ=255)
                                                                                                                         gATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTAACATGc                                                                                                                                                    >  1:2618841/1‑288 (MQ=255)
                                                                                                                          aTCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGcc                                                                                                                                                   >  1:179695/1‑288 (MQ=255)
                                                                                                                           tCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGCAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGaacgtcaacg                                                                                                                                                            >  1:1836252/1‑278 (MQ=255)
                                                                                                                            cATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGCAGCAGAACGTCAACGTCACCTGCCGCt                                                                                                                                                >  1:1192783/1‑289 (MQ=255)
                                                                                                                                             tGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTAc                                                                                                                                                                                                        <  2:1335399/216‑1 (MQ=255)
                                                                                                                                              gATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCtg                                                                                                                              <  1:1322104/289‑1 (MQ=255)
                                                                                                                                                tCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGa                                                                                                                                                                                               <  2:1230027/222‑1 (MQ=255)
                                                                                                                                                    gCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCa                                                                                                                        >  1:1519065/1‑289 (MQ=255)
                                                                                                                                                     cTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCAAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCACTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCGGGGGGCaa                                                                                                                       >  1:727723/1‑289 (MQ=255)
                                                                                                                                                      tCATCCGGCTTTGCGCATTTAACGGTACAAGGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAAt                                                                                                                      <  1:213828/289‑1 (MQ=255)
                                                                                                                                                         tCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACgg                                                                                                                                                                                                            >  2:510561/1‑200 (MQ=255)
                                                                                                                                                                  gcgcAGTAAACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTcgcg                                                                                                            >  1:2676229/1‑287 (MQ=255)
                                                                                                                                                                  cgccactAAACGCTACAAAGGGGGCACGGAGCGAGTGTACAT‑GCGACTCGCGCAGGTTAGAACGCTGCTTGGCGGGGATCACAATACTGCGGCCTTCCGGCGTTTCATCCGCCAGCGAAGCCGGTTGTGCGGCGTCAGACAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCCACGCCTGACGGTTACCGAGTGTGATGGTTCCCGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGcc                                                                                                          <  2:839435/283‑1 (MQ=255)
                                                                                                                                                                                aCAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCaa                                                                                                                       >  1:945194/1‑262 (MQ=255)
                                                                                                                                                                                aCAAAGGTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGGGGCCATCCCATTCGCCGCTAGCATCCGGccc                                                                                             >  2:2402476/1‑286 (MQ=255)
                                                                                                                                                                                      gTGGCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGcc                                                                                                                                                                                                                                           <  1:1004702/140‑1 (MQ=255)
                                                                                                                                                                                         gCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTa                                                                                                                                                                                                         <  2:1399822/171‑1 (MQ=255)
                                                                                                                                                                                         gCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGCTGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGGCACCTGCCGCTTCAACTTCACGTCCGCTGGTGGCAATCACACTCCCGCCTCGCATCCGGCTCCTCCCGGCg                                                                                    >  2:1655961/1‑288 (MQ=255)
                                                                                                                                                                                         gCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGc                                                                                                                                                    <  2:211130/224‑1 (MQ=255)
                                                                                                                                                                                         gCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAAt                                                                                                                      <  2:594039/254‑1 (MQ=255)
                                                                                                                                                                                         gCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCg                                                                                    >  1:1412268/1‑288 (MQ=255)
                                                                                                                                                                                         gCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCg                                                                                    >  2:1697734/1‑288 (MQ=255)
                                                                                                                                                                                         gCATGGAGCGACTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTCCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCCCGTCCGCCGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCg                                                                                    >  2:2577491/1‑288 (MQ=255)
                                                                                                                                                                                               agcgactGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCAAGTGTGATGGTGCCGGTTTTATCCAg                                                                                                                                                                            <  1:1989881/189‑1 (MQ=255)
                                                                                                                                                                                               agcgactGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCAAGTGTGATGGTGCCGGTTTTATCCAg                                                                                                                                                                            >  2:1989881/6‑194 (MQ=255)
                                                                                                                                                                                                gcgactGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGa                                                                             >  1:518821/5‑288 (MQ=255)
                                                                                                                                                                                                   actGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCCCGTTCGCTGGTGGCAATCACATTCGCCCCTAGCATCCGGCCTATCCCGGCCACGCCGACCg                                                                          >  2:1295293/2‑288 (MQ=255)
                                                                                                                                                                                                   actGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATc                                                                           >  1:428386/2‑287 (MQ=255)
                                                                                                                                                                                                   actGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATc                                                                           >  1:229780/2‑287 (MQ=255)
                                                                                                                                                                                                            cggg‑ctcgcgcAGGTGAACACGCTGCTTGGCGAGGATCAAAATACTGCCGCCTTCCGGCGTTTCATCGGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAg                                                                <  2:727051/286‑1 (MQ=255)
                                                                                                                                                                                                            cgcg‑ctcgcgcAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGcc                                                                                                          <  1:1573755/247‑1 (MQ=255)
                                                                                                                                                                                                            cgcg‑ctcgcgcAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAg                                                                >  1:981460/1‑289 (MQ=255)
                                                                                                                                                                                                                  tcgcgcAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTGATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCt                                                                                                                                                >  2:70584/1‑204 (MQ=255)
                                                                                                                                                                                                                  tcgcgcAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGTTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTCGCACTCACATTCACGCCTCGCATCCGGCTCATCCCGTCGCTGCCGATCGCTGCGAACAGgccg                                                            >  2:1176275/1‑288 (MQ=255)
                                                                                                                                                                                                                  tcgcgcAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGgccg                                                            >  2:545825/1‑288 (MQ=255)
                                                                                                                                                                                                                        aGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATa                                                      <  2:2622003/288‑1 (MQ=255)
                                                                                                                                                                                                                            tAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                 <  1:494557/289‑1 (MQ=255)
                                                                                                                                                                                                                                    tgctTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCCATAGTGGTTGGGATca                                         >  1:276905/1‑289 (MQ=255)
                                                                                                                                                                                                                                    tgctTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATca                                         >  1:2111019/1‑289 (MQ=255)
                                                                                                                                                                                                                                    tgctTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGGCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGa                                            >  2:619739/1‑286 (MQ=255)
                                                                                                                                                                                                                                      ctTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCACCTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATcag                                        >  2:1785638/1‑288 (MQ=255)
                                                                                                                                                                                                                                       ttGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAACCTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATcaga                                       <  1:345257/288‑1 (MQ=255)
                                                                                                                                                                                                                                                aTCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACcagc                              <  1:1249081/288‑1 (MQ=255)
                                                                                                                                                                                                                                                  cacaATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCagcagc                           <  1:2219332/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaTCCTGCGGACGTCCGGGGTTTCATCAGCCAGCGAGGCCGGTTGTGCGGCGTCCGCCAGCGTTTTTTCTTCCAGGGCCTGGGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTCTCCAGTAGCAGAACGTCAACGTCCCCTGCCGCTTCAACTGCACGTCCGCTGGTGGCCATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGcc                        <  2:2108782/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          tGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAg                   >  1:749343/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCGACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGAGCAGAAACACCAGCAGCGCAACCAGTa                 >  2:1364907/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGtt                                                                                                                                                                                                          >  1:627773/1‑104 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGa                                                                                                                                                                                               <  2:2664640/115‑1 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTcg                                                                                                              >  1:488827/1‑196 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTcg                                                                                                              <  2:488827/196‑1 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCgg                                                                                                <  2:2277040/210‑1 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCgg                                                                                                >  1:2277040/1‑210 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACACCAGGCCGCCAATAGCGGTTGGGATCCGACAGACCAGCCGCGCCACCAGt                  >  2:753156/1‑288 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAgg                                                               <  1:1707297/243‑1 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAg                                                     >  2:2448344/1‑253 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAg                                                     <  1:2448344/253‑1 (MQ=255)
                                                                                                                                                                                                                                                             ggCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGAGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTACGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCGAGTAGGGGTTGGGATCAGACAGACCAGCAGCGCCACCTGTa                 >  1:2101527/1‑288 (MQ=255)
                                                                                                                                                                                                                                                               ccTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATca                                                                                                                    >  2:894100/1‑187 (MQ=255)
                                                                                                                                                                                                                                                               ccTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCg              >  1:531862/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               ccTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAAGCACATTCGCCCCGAGCCTCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGCTGGGATTAGAACGCACAGCAGCCCCAGCAGtcgcg              >  2:1255041/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                 ttCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACTTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCCGCCCCTCCCGGCGTCGCCGATCGCTGCCAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGAACCCCCACCATTACCgg             >  2:1560674/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                  tCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                 >  1:1377463/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                  tCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCCACTCCCGGCGACGCCGATCGCTGACAACAGGGCGCCACTACTGGTTGGGATCAGACAGACCCGGCACGCCACCAGTAcgc              >  2:1603692/1‑284 (MQ=255)
                                                                                                                                                                                                                                                                   ccGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGa                                                                             <  1:763604/222‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCt       <  1:876937/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                   
CCACCACCAGCGGCGTGGCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCGCAGTAAACGGTACAAAGGTGGCATGGAGGCTCTGCACATCGCG‑CTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGGCTAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCGCTCGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCACTCAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTG  >  CP042583/598169‑598723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A