Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 640,172 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583640,1720AT100.0% 520.4 / NA 216intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (81/135);  total (81/135)
*CP042583640,1730GC99.1% 514.7 / NA 216intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base C (80/134);  total (81/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583640,1740TA100.0% 518.2 / NA 216intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (81/135);  total (81/135)

Only 100 of 217 total aligned reads displayed.

AGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTAGCCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCAAGTGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCAAGCTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGA  >  CP042583/639886‑640453
                                                                                                                                                                                                                                                                                                  |||                                                                                                                                                                                                                                                                                       
         agagAAAGCTGCAGCCGAAAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGAA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGat                                                                                                                                                                                                                                                                                <  1:2386322/289‑1 (MQ=255)
         aaagaAAACTGGAGCGGACAAAAAGGAAGCAGAAGAAAGGGCTGCTGAAAAGGAAGCA‑GCTGAT‑AGAAAGGAGACGGCAGAAAAAGCCGCAGCAGACAAAAAGGGGGCAGCGGAAAAAGAGGCAGCTGAAAAAGGAGCGGCAGCAAAAGGGGACGCAGA‑GGCAGATGGTATTTTAGGTGAGCTA‑AGCTCTGGTAAGAATGAACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCg                                                                                                                                                                                                                                                                                  <  2:2078811/285‑1 (MQ=255)
              aagaggCAGCGGACAAAAAAGCAGCAGAAAAAGCGGCGGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGAAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATc                                                                                                                                                                                                                                                                             <  2:829103/282‑1 (MQ=255)
              aaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATc                                                                                                                                                                                                                                                                             <  1:50397/287‑1 (MQ=255)
                  tGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATa                                                                                                                                                                                                                                                                         >  1:2634006/1‑287 (MQ=255)
                           cAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCggg                                                                                                                                                                                                                                                              <  1:1647128/289‑1 (MQ=255)
                           cAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCgg                                                                                                                                                                                                                                                               >  1:1750282/1‑288 (MQ=255)
                           cAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAAAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCggg                                                                                                                                                                                                                                                              <  1:2355185/289‑1 (MQ=255)
                           cAAAAAACCAGAAGAAAAAGCGGCTGAGGAAAAGGCAGAA‑TGGGATAAGACAGCAA‑CGGCAGAAAAAGGCTCCGTAGCAAAAAGAGCGGCAGCGGGAAAAACTGCAGCGGAAAAAGCCGCGGCAGCAGAGGCCGCCGCAGA‑GGCAGATGATATTTTCGTTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTGATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCggg                                                                                                                                                                                                                                                              <  2:1291861/289‑1 (MQ=255)
                           aaaaaaaGCAGCCGAAAAAGCGGATGCTGAAAAGGCAGCA‑GCGGAGAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCggg                                                                                                                                                                                                                                                              <  2:1175877/288‑1 (MQ=255)
                            aaaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATc                                                                                                                                                                                                                                                                             >  2:2639398/1‑273 (MQ=255)
                                    aacagAAAAAACGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑GGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTa                                                                                                                                                                                                                                                       <  1:1882563/285‑1 (MQ=255)
                                          aaaaGCGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTg                                                                                                                                                                                                                                                 <  1:1382805/287‑1 (MQ=255)
                                          aaaaGCGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTg                                                                                                                                                                                                                                                 <  1:412164/287‑1 (MQ=255)
                                                  ctgctgAAAAGGCAGCA‑GATGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGaaa                                                                                                                                                                                                                                        <  1:2648473/288‑1 (MQ=255)
                                                   tgctgAGAAGG‑AGCAGGCAGAAAAGAAAGCAG‑GGGCAGACAGAGCCGCCGCAGACAAATAAGCAGCAGCGGAAAAAGCGGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCGGA‑GGCAGATAATATTTTCGGGGAGCTA‑AGCTCTGGTAAGAATGCACCGGAAACGGGGGGAGGTGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAaagt                                                                                                                                                                                                                                      <  2:211494/289‑1 (MQ=255)
                                                   tgctgAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAaagt                                                                                                                                                                                                                                      <  1:1357618/289‑1 (MQ=255)
                                                   tgctgAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAaagt                                                                                                                                                                                                                                      <  1:2216438/289‑1 (MQ=255)
                                                   tgctgAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAA‑GGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGATACAGAaagta                                                                                                                                                                                                                                     >  2:2006792/1‑289 (MQ=255)
                                                               agca‑gcTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGAc                                                                                                                                                                                                                          >  1:689441/1‑289 (MQ=255)
                                                                  a‑gcTGATAAGAAAGCAG‑CGGCAGAAACAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGAGGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCa                                                                                                                                                                                                                       <  1:434398/289‑1 (MQ=255)
                                                                  a‑gcTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCa                                                                                                                                                                                                                       <  2:1014291/289‑1 (MQ=255)
                                                                  a‑gcTGATAAGAAAGCAG‑CGGAAGAAAAGGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCa                                                                                                                                                                                                                       <  1:1287550/289‑1 (MQ=255)
                                                                  a‑gcTGAAAAGAAAGAAG‑AGGCAGAAAAGGCCGCGGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCa                                                                                                                                                                                                                       <  2:149798/289‑1 (MQ=255)
                                                                    gcTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCGGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCa                                                                                                                                                                                                                       <  1:2606164/288‑1 (MQ=255)
                                                                     cTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGat                                                                                                                                                                                                                                                                                >  2:1066122/1‑230 (MQ=255)
                                                                      tGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCa                                                                                                                                                                                                                       <  1:1894495/286‑1 (MQ=255)
                                                                      ggATAAGAAAGCAG‑AGGAAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCa                                                                                                                                                                                                                       <  1:1985409/285‑1 (MQ=255)
                                                                        aTAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAaagt                                                                                                                                                                                                                                      >  2:1195140/1‑269 (MQ=255)
                                                                             aagacag‑aGGCAGAAAAAGCCGCGGAAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTAAAAAAACCGCGGAAGCAAAAGGGGACGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCGTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATg                                                                                                                                                                                                             <  2:80016/285‑1 (MQ=255)
                                                                                  ag‑cGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGc                                                                                                                                                                                                        >  2:979411/1‑289 (MQ=255)
                                                                                     cGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAg                                                                                                                                                                                                                                                           >  2:1712830/1‑236 (MQ=255)
                                                                                     cGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAg                                                                                                                                                                                                                                                           <  1:1712830/236‑1 (MQ=255)
                                                                                     cGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCaa                                                                                                                                                                                                      >  2:2328013/1‑289 (MQ=255)
                                                                                     cGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCaa                                                                                                                                                                                                      >  1:1499128/1‑289 (MQ=255)
                                                                                      ggCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCaa                                                                                                                                                                                                      <  2:673533/288‑1 (MQ=255)
                                                                                      ggCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCaa                                                                                                                                                                                                      <  2:865034/288‑1 (MQ=255)
                                                                                      ggCAGAAAAAGCCGCCGAAGAAAAAAAAGCGGCAGCGGAAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGTTATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCaa                                                                                                                                                                                                      <  2:2252298/288‑1 (MQ=255)
                                                                                      ggCAGAAAAAGCCGCCACAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGc                                                                                                                                                                                                        <  1:2668047/286‑1 (MQ=255)
                                                                                         aGAAAAAGCCGCAGCAGACAAAAAAACGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAAcc                                                                                                                                                                                                  <  1:1088740/289‑1 (MQ=255)
                                                                                               aGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGCTGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAAAGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGCAATACTAAAAACAATGGCGCATGAGCGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTTAGTTCGATGACGCATCGTCCTATGGAGGCAAAACCtttcc                                                                                                                                                                                             >  2:329054/1‑284 (MQ=255)
                                                                                               aGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACg                                                                                                                                                                                            <  1:2075874/289‑1 (MQ=255)
                                                                                                     cgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATg                                                                                                                                                                                                                            >  1:2187304/1‑251 (MQ=255)
                                                                                                                   cggcagcggcaAAAGCTGAAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCAc                                                                                                                                                                         <  1:1814341/288‑1 (MQ=255)
                                                                                                                    ggcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCAcc                                                                                                                                                                        <  1:745959/288‑1 (MQ=255)
                                                                                                                        gcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATg                                                                                                                                                                   >  2:2503676/1‑289 (MQ=255)
                                                                                                                          ggcaAAAGCTGCAGCTGAAAAGGCCGCTGCAGCAAAAGCGGGCGCAGA‑AGCAGATGATATTTTCGGTGGGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATg                                                                                                                                                                   <  2:2611452/287‑1 (MQ=255)
                                                                                                                          gacaAAAACTGCAGCTGAAACAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GTCAGATGATATTTTCGGTTAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGTGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGTGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATTCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATg                                                                                                                                                                   <  1:1674594/285‑1 (MQ=255)
                                                                                                                           gacagaggttcAGCTGAAAAAGCCGCTGCAGGAAAAGGGGCCGCAGA‑GGCAGATGAGTTTTTGGGTGGGCTA‑AGCTATGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATTACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGt                                                                                                                                                                 <  2:1773070/278‑1 (MQ=255)
                                                                                                                            caAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGGCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTa                                                                                                                                                                <  1:1488969/288‑1 (MQ=255)
                                                                                                                            caAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTa                                                                                                                                                                <  2:695599/288‑1 (MQ=255)
                                                                                                                            caAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTa                                                                                                                                                                <  1:1783051/288‑1 (MQ=255)
                                                                                                                                   tGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTg                                                                                                                                                        >  1:363248/1‑289 (MQ=255)
                                                                                                                                   tGCAGCTGAAAAAGCCGCTGAAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTg                                                                                                                                                        <  1:52651/289‑1 (MQ=255)
                                                                                                                                                 ccGCGGCAGCCAAAGGGGCCG‑AGAGGGCAGGGGATATGTTCGTGGGTATA‑AGTTCTGGTAAGACTGCACCGAGAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAAGACTAAAAACAATGGGGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTg                                                                                                                                           <  2:687961/288‑1 (MQ=255)
                                                                                                                                                  cGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTg                                                                                                                                           <  1:979411/287‑1 (MQ=255)
                                                                                                                                                                  gCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGtt                                                                                                                                                                                                                                 <  2:1699296/185‑1 (MQ=255)
                                                                                                                                                                                atgatATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCggg                                                                                                                                                                                                                                                              <  1:1066916/143‑1 (MQ=255)
                                                                                                                                                                                      tgtgcGGGGAGCTAGAGCT‑TGGAAAGAAGGGAACGAAAACGGGGGGATGGGAGAAAGGGAAGAATACTGCTCCTGCCGAGAGTGGTAATACTAAAAACAATGGGGCGTCAGGGGCCGATATCAATAACTATGCCGGGCGGATTAAAGCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGgc                                                                                                      <  2:908207/285‑1 (MQ=255)
                                                                                                                                                                                        ttCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTgg                                                                                                                                    <  1:2425183/257‑1 (MQ=255)
                                                                                                                                                                                        ttCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTgg                                                                                                                                    >  2:2425183/1‑257 (MQ=255)
                                                                                                                                                                                              gAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTa                                                                                              >  2:1832960/1‑289 (MQ=255)
                                                                                                                                                                                              gAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACCGGCACCCGATGGTATTGTACTGGAAATCAAAACGGAAGGTGGCGATCCCTCACCTTGTCCGGCTTCCTTGGCATCAACTa                                                                                              >  2:77987/1‑289 (MQ=255)
                                                                                                                                                                                                gCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTaa                                                                                             >  2:2549013/1‑288 (MQ=255)
                                                                                                                                                                                                          tGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTg                                                                                                                                                        <  2:578516/220‑1 (MQ=255)
                                                                                                                                                                                                           ggTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGa                                                                                  >  1:1193849/1‑289 (MQ=255)
                                                                                                                                                                                                            gTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGa                                                                                  >  1:1166453/1‑288 (MQ=255)
                                                                                                                                                                                                                    gCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGGTGGCAGCAGCTAAACTTTCGAAAATCCCGaaa                                                                          >  2:2687082/1‑288 (MQ=255)
                                                                                                                                                                                                                      aCCGAAACCGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTTGTAAAACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCGGCTAAACTTGCGAAGATCCCGAAacca                                                                       <  1:723456/289‑1 (MQ=255)
                                                                                                                                                                                                                      aCCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGt                                                                                                                    >  2:2366964/1‑244 (MQ=255)
                                                                                                                                                                                                                      aCCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTa                                                                                              <  2:1376925/266‑1 (MQ=255)
                                                                                                                                                                                                                      aCCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAacc                                                                        >  1:2299264/1‑288 (MQ=255)
                                                                                                                                                                                                                           aaaCGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCa                                                                                                                                                                                                                       >  1:2327198/1‑140 (MQ=255)
                                                                                                                                                                                                                             aCGGGGGGAGGGGCGAAAGGGACCAATGCTTCGCATGACGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAaccacc                                                                     <  2:1526477/284‑1 (MQ=255)
                                                                                                                                                                                                                               ggggggAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAg                                                              >  1:846731/1‑289 (MQ=255)
                                                                                                                                                                                                                                gggggAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAg                                                              >  1:1705110/1‑288 (MQ=255)
                                                                                                                                                                                                                                  ggggggggCGAAAGGGAGAAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGAGGCCGATATCAAGAACTAGGCCGGGCAGATGAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGc                                                            <  2:1935644/288‑1 (MQ=255)
                                                                                                                                                                                                                                  gggAGGGGCGAAAGGGAACAATGCTTCGCTTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGc                                                            <  1:1416454/288‑1 (MQ=255)
                                                                                                                                                                                                                                  gggAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGc                                                            <  1:2040526/288‑1 (MQ=255)
                                                                                                                                                                                                                                  gggAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGc                                                            <  1:2549013/288‑1 (MQ=255)
                                                                                                                                                                                                                                  gggAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGc                                                            <  2:2299264/288‑1 (MQ=255)
                                                                                                                                                                                                                                  gggAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGc                                                            <  1:1832960/288‑1 (MQ=255)
                                                                                                                                                                                                                                  gggAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCa                                                           >  1:2447344/1‑289 (MQ=255)
                                                                                                                                                                                                                                    gAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCa                                                                                                                                                                                                                       <  2:414249/131‑1 (MQ=255)
                                                                                                                                                                                                                                       gggCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGAGATACCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCa                                                           >  1:1526477/1‑284 (MQ=255)
                                                                                                                                                                                                                                           gAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCa                                                                                                                          <  1:782240/217‑1 (MQ=255)
                                                                                                                                                                                                                                                    cAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCAcc                                                                                                                                                                        >  2:945357/1‑162 (MQ=255)
                                                                                                                                                                                                                                                    cAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCAcc                                                                                                                                                                        <  1:945357/162‑1 (MQ=255)
                                                                                                                                                                                                                                                          ttcgccTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAAcgc                                    >  1:1964928/5‑288 (MQ=255)
                                                                                                                                                                                                                                                               cTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCAtt                              <  1:1026144/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ccGGGAGTGGTAATACTGAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCg                                                                                                                                                                      <  2:221182/150‑1 (MQ=255)
                                                                                                                                                                                                                                                                            tAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTCAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACAACCAAGCCAGGCAGTCTATGAAGTGTTCAAAAACGCGCCCTTGGACTTCAAAcc                  >  2:1892064/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                    aaaaCAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATcgcg         >  2:886489/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                         aaTGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGtt    >  1:583886/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                          aTGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATg                                                                                                                                                                   <  2:2445875/129‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          aTGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTg   >  1:2245358/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                           tGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGc                                                            <  1:2345029/231‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           tGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCAtt                              >  1:928375/1‑261 (MQ=255)
                                                                                                                                                                                                                                                                                           tGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTg   >  1:2641541/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                           tGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGa  >  2:1123567/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                                  |||                                                                                                                                                                                                                                                                                       
AGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCA‑GCTGATAAGAAAGCAG‑CGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGA‑GGCAGATGATATTTTCGGTGAGCTA‑AGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTAGCCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCAAGTGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCAAGCTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGA  >  CP042583/639886‑640453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A