Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 659,209 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583659,2090AT99.6% 649.6 / NA 274intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (133/139);  total (133/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583659,2100GC99.6% 642.3 / NA 274intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (132/141);  total (133/141)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583659,2110CG99.3% 634.5 / NA 274intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base G (130/140);  total (133/141)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 274 total aligned reads displayed.

TCTGTATGACTGGTTTGGTATTACCTTCGCCTTTAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGAGCTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGAGCAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTAGTGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGCTATCTTTGGCGTCTCCG  >  CP042583/658932‑659487
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                      
tCTGTATGACTGGTTTGGTATTACCTTCGCCTTTAGTTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACAtt                                                                                                                                                                                                                                                                                  >  1:1836127/1‑286 (MQ=255)
          ttgTTTGGTATTACCTTCGCCTTTAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAAcgc                                                                                                                                                                                                                                                                     <  1:2119013/287‑1 (MQ=255)
          tggtttggttTTACGTTCGCCGTTGGCTGGAGCGGCGCGGTGCTCGCTGCGGCCGTCATGTCGTTTCAGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAAcg                                                                                                                                                                                                                                                                      <  2:1689310/288‑1 (MQ=255)
           ggTTTGGTATTACCTTCGCCTTTAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTGGTGTCGAACATTCCTGGTGaaaggtg                                                                                                                                                                                                                                                                    >  2:205998/1‑285 (MQ=255)
                      aCCTTCGCCTTTAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCtt                                                                                                                                                                                                                                                         <  2:256457/289‑1 (MQ=255)
                       ccTTCGCCTTTAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCt                                                                                                                                                                                                                                                          >  1:2085006/1‑287 (MQ=255)
                          tcggcttgtgCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTGATGTCGTTGCCGCTGTTGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                                                                                                                                                                                                                                     <  2:1151248/280‑1 (MQ=255)
                          tCGCCTTTAGCTGGGGGGGCGCGGTTCTCGCTGCCGCCGTAATGTCGTTTCCGCTGATGGGGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                                                                                                                                                                                                                                     <  2:217395/289‑1 (MQ=255)
                          tCGCCTTTAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                                                                                                                                                                                                                                     <  1:27114/289‑1 (MQ=255)
                          tCGCCTTTAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                                                                                                                                                                                                                                     <  1:526870/289‑1 (MQ=255)
                          tCGCCGTTAGATGGGGCGGGCCGGTGCTAGGTGCCGGCGTTATGCCGTTTCCGCGCGGGGCGCAGGCATTTGGCCTGGCTCTGGAACAGGTTGTTGTCCAACTGGATCAGGCCGCAATAACAGTGGGGGGCGCGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTGCCGCGAATTCTTGTTGCTACGGTAATGGCTTTCGCCCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGACGAACATTCCTGGTGAAACGTGAACCATTCCTTCTGc                                                                                                                                                                                                                                                     <  2:383034/289‑1 (MQ=255)
                              cTTTAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCAt                                                                                                                                                                                                                                                  <  1:680488/288‑1 (MQ=255)
                                ttAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGta                                                                                                                                                                                                                                               <  2:1356384/289‑1 (MQ=255)
                                 tAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGtat                                                                                                                                                                                                                                              >  1:2062960/1‑289 (MQ=255)
                                     tGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAc‑c                                                                                                                                                                                                                                          <  1:783229/288‑1 (MQ=255)
                                     tGGCGCGGCACGGTTCTCGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAc‑c                                                                                                                                                                                                                                          <  1:1432394/288‑1 (MQ=255)
                                            gcggggTTGTGGCTGCGGGGGTCATGTCGTTTCCGCTGATGGTGCGGGAAATTCGTCTGGCGGTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATcc                                                                                                                                                                                                                                   <  2:2295953/288‑1 (MQ=255)
                                                      cGCTGCCGCCGTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCcggc                                                                                                                                                                                                                        <  1:2331683/289‑1 (MQ=255)
                                                            cgccgTCATGTCGTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCCTTTTCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCCTACATTCCTGGTGAAACGCGAACCATGCCTTCTGCCATGT‑TACACCGTATCCCAGCACCCGGCGGGGaa                                                                                                                                                                                                                  >  2:2331683/1‑289 (MQ=255)
                                                                  catgtcgtttCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTgg                                                                                                                                                                                                             <  1:2566240/281‑1 (MQ=255)
                                                                  catgtcgtttCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTgg                                                                                                                                                                                                             <  2:2636780/281‑1 (MQ=255)
                                                                   atgtcgtttCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAg                                                                                                                                                                                                           <  1:334739/283‑1 (MQ=255)
                                                                          ttCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTGGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCga                                                                                                                                                                                                     <  1:2690248/288‑1 (MQ=255)
                                                                                            cAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTa                                                                                                                                                                                   <  2:935868/288‑1 (MQ=255)
                                                                                                tctgcTGGCGCTGGAGTGGGTGGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTCCTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTgcg                                                                                                                                                                              <  2:504864/285‑1 (MQ=255)
                                                                                                tCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTgcg                                                                                                                                                                              <  1:307112/289‑1 (MQ=255)
                                                                                                tCGGCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCTGAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTgcg                                                                                                                                                                              <  2:43390/289‑1 (MQ=255)
                                                                                                        cgcTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCga                                                                                                                                                                       <  1:1611567/288‑1 (MQ=255)
                                                                                                               aGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTcc                                                                                                                                                               <  1:1549942/289‑1 (MQ=255)
                                                                                                                         gTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCgaga                                                                                                                                                                                                   >  2:1506733/1‑243 (MQ=255)
                                                                                                                                  gAACAGGCCGCAAGGACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGcc                                                                                                                                                                                                                                                    <  2:304829/186‑1 (MQ=255)
                                                                                                                                  gAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAAtgg                                                                                                                                             <  2:171069/288‑1 (MQ=255)
                                                                                                                                        gCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                                                                                                                                                                                                                                     >  1:948127/1‑179 (MQ=255)
                                                                                                                                        gCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCcggc                                                                                                                                                                                                                        <  2:2355942/207‑1 (MQ=255)
                                                                                                                                        gCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAAtggc                                                                                                                                            <  1:1718480/283‑1 (MQ=255)
                                                                                                                                        gCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCa                                                                                                                                      >  1:1728603/1‑289 (MQ=255)
                                                                                                                                        gCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTGGATTTCAGAATGGCTGGCCa                                                                                                                                      >  1:2445583/1‑289 (MQ=255)
                                                                                                                                        gCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTCCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTTCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCa                                                                                                                                      >  1:2174234/1‑289 (MQ=255)
                                                                                                                                         ccGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAg                                                                                                                                     >  1:445122/1‑289 (MQ=255)
                                                                                                                                          cGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGta                                                                                                                                                                                                                                               <  1:2213615/183‑1 (MQ=255)
                                                                                                                                          cGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGc                                                                                                                                                                         <  2:1000711/252‑1 (MQ=255)
                                                                                                                                          cGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGc                                                                                                                                                                         >  1:1000711/1‑252 (MQ=255)
                                                                                                                                             aaGAACCCGGGCGGCCGGGGGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAAt                                                                                                                                  <  2:673437/288‑1 (MQ=255)
                                                                                                                                             aaGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                                                                                                                                                                                                                                     >  1:979153/1‑174 (MQ=255)
                                                                                                                                                  cacTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                                                                                                                                                                                                                                     >  1:1810575/1‑169 (MQ=255)
                                                                                                                                                  cacTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                                                                                                                                                                                                                                     <  2:1810575/169‑1 (MQ=255)
                                                                                                                                                  cacTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCTCTTGCTATGATCTCCCTGTTGATTTCAGAATGTCTGGTCCGAATTCGCCg                                                                                                                            >  1:1530320/1‑289 (MQ=255)
                                                                                                                                                  cacTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTGGATTTCAGAATGGCTGGCCAGAAACAGCCg                                                                                                                            >  1:63699/1‑289 (MQ=255)
                                                                                                                                                    cTGGGGGCCGGGCGCTGGCGCGGTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCgaga                                                                                                                                                                                                   <  1:2401070/216‑1 (MQ=255)
                                                                                                                                                       ggggCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGaa                                                                                                                        <  1:2543790/288‑1 (MQ=255)
                                                                                                                                                            cGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAAcgggcg                                                                                                                  <  1:1876446/289‑1 (MQ=255)
                                                                                                                                                             gggCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAAcgggcg                                                                                                                  <  1:1796376/288‑1 (MQ=255)
                                                                                                                                                              ggCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAAcgggcggg                                                                                                                >  2:1656475/1‑289 (MQ=255)
                                                                                                                                                              ggCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGCGGAAAGCGGAGCGGCGAGCCtgtcactta                                                                                                                                                                                         >  2:2401070/1‑210 (MQ=255)
                                                                                                                                                                   tGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATAAGCCGTGAACGGGCGGGGCGCt                                                                                                           >  1:2266966/1‑289 (MQ=255)
                                                                                                                                                                                ttACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGaa                                                                                             <  1:1385609/289‑1 (MQ=255)
                                                                                                                                                                                    tATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCC‑TGCTGGAACTGa                                                                                         >  1:1466032/1‑289 (MQ=255)
                                                                                                                                                                                        aCGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCACCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTCCTATTGCGCTGGCGATGATCTCCCTGTGTATTTCAGCACGCCTGGCTAGAACCAGCCGTGAACGGTCGGGGCGCTAACCATTGCT‑GAACTGAAatt                                                                                     >  2:2739699/1‑289 (MQ=255)
                                                                                                                                                                                         cGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCgaga                                                                                                                                                                                                   >  2:160099/1‑179 (MQ=255)
                                                                                                                                                                                             aCCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGaa                                                                                                                        >  2:172467/1‑250 (MQ=255)
                                                                                                                                                                                             aCCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTccc                                                                                >  1:1227661/1‑289 (MQ=255)
                                                                                                                                                                                                gCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGa                                                                             <  2:2539268/289‑1 (MQ=255)
                                                                                                                                                                                                      cTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTgg                                                                       >  1:1029936/1‑289 (MQ=255)
                                                                                                                                                                                                         aCCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTTATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACCGGCGGGGCGCTAACCA‑TGCTGGAATTGCATTTTTccccacccttggtc                                                                     >  2:951312/1‑277 (MQ=255)
                                                                                                                                                                                                                         ggtacggtacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGAt                                                                                                                                                       <  1:1589294/191‑1 (MQ=255)
                                                                                                                                                                                                                         ggtacggtacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGAGCCTCCTGTTGATTTTAGAATGGCTGGCCAGAATCAGCCGGGAACGGGCGGGGTGCTCAACA‑TGCGGGAACTGAATTTTTCCCAGACGTTGGGCAACCCTTGCCTGACCAt                                                    >  2:2728331/1‑289 (MQ=255)
                                                                                                                                                                                                                            acggtacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTaa                                                 >  1:608998/1‑289 (MQ=255)
                                                                                                                                                                                                                               gtacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGTCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGa                                              >  1:1362073/1‑289 (MQ=255)
                                                                                                                                                                                                                                tacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTAt                                                    <  1:2373399/282‑1 (MQ=255)
                                                                                                                                                                                                                                   tGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGaaa                                                                                                                                                                                                                 <  1:2513469/123‑1 (MQ=255)
                                                                                                                                                                                                                                   tGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGaaa                                                                                                                                                                                                                 >  2:2513469/1‑123 (MQ=255)
                                                                                                                                                                                                                                     gCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGACTTTTTCACAGACGTTGGGCAACCATTGCCTGACTATTAAAGTAAc                                           >  2:2030863/1‑286 (MQ=255)
                                                                                                                                                                                                                                         ttGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGccc                                    <  1:1656475/289‑1 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGATTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAAACAGCCGTGAACGGGCCGGGCCCTAATCA‑TGATGGAACTGAATTTTTCCCAGACCGTGGGCACACCTTGCCCGAATATTTATGAAACGcccgcc                                    >  2:1856520/1‑283 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCa                                                                                                                                                                                                                                                               <  2:3938/71‑1 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCa                                                                                                                                                                                                                                                               >  1:3938/1‑71 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGcc                                                                                                                                                                                                                                                    <  2:846888/82‑1 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGcc                                                                                                                                                                                                                                                    >  1:846888/1‑82 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGAGTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATTA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAATGCTGccc                                    >  1:358749/1‑288 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TTCTGGAACTGAATTTTGCGCAGACGTTGTGCAACCAGTGCCTGACTATTAATGAAACGCAGccc                                    >  2:1437801/1‑288 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCa                                                                               <  1:1191101/245‑1 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCa                                                                               >  2:1191101/1‑245 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACTTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGccc                                    >  1:1126295/1‑288 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCg                                   >  1:88950/1‑289 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGGTGGGCAACCATTGCCTGACTATTAATGAAACAGTGccc                                    >  2:2127212/1‑288 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGAGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGTTTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTACTAA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGTCTACCATGGACTGCCTATTAATGAACCGCTGccg                                    >  2:1779798/1‑287 (MQ=255)
                                                                                                                                                                                                                                          tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGAACCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCCTTATTTCTATTGCGCTGGCGATGATCACCCTGTTGATTTAAGAATGGCTTGCCAGAATCAGCCGTGAACGGGCTGGGCGCTAATCA‑GGCTGGTACTGAATTTTTCCAATACGCTGTGCAACCACGCCCACACTATTACTGAAACGCTGccg                                    >  2:1880496/1‑287 (MQ=255)
                                                                                                                                                                                                                                               gTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGt                                                                                                                                                           >  1:723569/1‑165 (MQ=255)
                                                                                                                                                                                                                                                    ctgggTGTGTTTGGTGCAACCATCACCTTTGTGTCGACCATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCAt                         <  2:15844/286‑1 (MQ=255)
                                                                                                                                                                                                                                                    ctcGGTGGGTTTGTGGCCACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCAGGTATAC‑CCTGATCCCGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCAt                         <  2:1397148/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    ctcGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCAt                         <  1:2023752/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    ctcGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCAt                         <  2:608998/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    ctcGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCAt                         <  1:125609/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    cgcgtggAGTTTGGTGCAACCAGCACCGTTGTGTCGAACATTCCTGGGGAAACGGGAGCCATTCCTTCGGCCATGTATAC‑CCTGATCCAGACCCCCGGGGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCAt                         <  2:626424/283‑1 (MQ=255)
                                                                                                                                                                                                                                                      cGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCa                       <  2:332876/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          gAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGc                   <  2:88950/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             tttGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGCTa                 >  1:282586/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                       ccATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGCTATCTTTGGCGt       >  1:1763246/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                       ccATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGAGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGCTATCTTTGGCg        >  2:723019/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                          tCACCTTTGTGTCGAACATTTCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATGTCCTGACTTTTAATTAAACGCTGCCCGCCCATGTCCTAACTGctctctctctttcttat   >  2:1957444/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                      
TCTGTATGACTGGTTTGGTATTACCTTCGCCTTTAGCTGGCGCGGCGCGGTTCTCGCTGCCGCCGTCATGAGCTTTCCGCTGATGGTGCGGGCAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGAGCAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAC‑CCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTAGTGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCA‑TGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGCTATCTTTGGCGTCTCCG  >  CP042583/658932‑659487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A