Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 672,454 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583672,4540AT98.1% 703.3 / NA 313intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (5/1);  new base T (130/174);  total (137/176)
Fisher's exact test for biased strand distribution p-value = 8.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583672,4550GC99.4% 725.5 / NA 313intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base C (134/176);  minor base T (2/0);  total (137/176)
Fisher's exact test for biased strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP042583672,4560CG99.7% 736.6 / NA 313intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (134/176);  total (137/176)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

CCCCATGGCTCACCGCCCACGCTGGACATTGAGCCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGAGCATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCAGCCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGAGCGCGCGCAAAGCGAAAGCGGCAGGAAGCACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCAGCCCGGAGTTTTACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCG  >  CP042583/672174‑672727
                                                                                                                                                                                                                                                                                          |||                                                                                                                                                                                                                                                                               
 cccATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACgcgc                                                                                                                                                                                                                                                                           <  1:1044714/288‑1 (MQ=255)
          cACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATgg                                                                                                                                                                                                                                                                 >  1:277916/1‑289 (MQ=255)
           aCCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATggg                                                                                                                                                                                                                                                                >  1:2122738/1‑289 (MQ=255)
           aCCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCGTCTGTATggg                                                                                                                                                                                                                                                                >  2:2675784/1‑289 (MQ=255)
             cGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGgcg                                                                                                                                                                                                                                                              <  2:2050624/289‑1 (MQ=255)
             cGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGgcg                                                                                                                                                                                                                                                              <  1:53027/289‑1 (MQ=255)
               cccACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGcgcg                                                                                                                                                                                                                                                            <  2:741329/289‑1 (MQ=255)
                 cACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAGACCGGGCTGGAAGCCGAGCGGTTGCTGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCgcggc                                                                                                                                                                                                                                                          >  1:1363805/1‑289 (MQ=255)
                  aCGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCATAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTGCGGTCTGGGCgcggcg                                                                                                                                                                                                                                                         >  2:298004/1‑289 (MQ=255)
                       ggACATTGTCGCAAGTCACAGGATTATTGGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCGTTTCTATCCTCCTCAGGTGAAGGTCAGCACGTTGC‑GGCCGATTAAGACCGCAGCTAGTCCGGAAGATTGCAAATACTGCCCGCAAAGCCCGCGCTACAAAACGGGCCGGGAAGCCGAGCGGCTGATGGAAGTATAACGAGGT‑CTGGAGTGGGCGCGCAAGTCTAACGCGTGAGGATCCACGCCCATCTGCGTGGGGGCGGTGTGgaa                                                                                                                                                                                                                                                    >  2:843417/1‑289 (MQ=255)
                          cctttgcccagttCACAGGATTATATGACAAACCGTTGCGGGATCTGCTGTTTGAAGCGCCGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGgaaga                                                                                                                                                                                                                                                  <  2:593933/276‑1 (MQ=255)
                             tgtcgcAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATc                                                                                                                                                                                                                                               >  1:1566820/6‑288 (MQ=255)
                             tgtcgcAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATc                                                                                                                                                                                                                                               >  1:1013695/6‑288 (MQ=255)
                              gtcgcAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCCGTATGGGCGCGGGGTGGGAGAGTcc                                                                                                                                                                                                                                              >  2:869390/5‑288 (MQ=255)
                                  cAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGAACGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACg                                                                                                                                                                                                                                         >  1:1541300/1‑289 (MQ=255)
                                       cacaGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCTGCGTGGAAGAATCCCACCGAAcgc                                                                                                                                                                                                                                    >  2:1371844/1‑289 (MQ=255)
                                             attattTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGat                                                                                                                                                                                                                                 <  1:412527/286‑1 (MQ=255)
                                                 tttGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGCGCTTGTCCGGAAGCTTGCAACTACTGCCCGCAAAGCCCGCGCTACAAACCCGGGCTGGAAGCCGAGCGGTTGCTGGAAATTAAAC‑AGGGGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGAACGACGCGCCTCGGGACTGGGGCGGCGGGGAAGAATCCCCCAGACCGCGGAATGCCg                                                                                                                                                                                                                           >  2:1617688/1‑288 (MQ=255)
                                                   tGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTAc                                                                                                                                                                                                                        <  1:1231618/289‑1 (MQ=255)
                                                        atcccttGCCGGGTCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGCTCAGAACGTTGC‑TGTCGATTAAGACCGGCGCTTGTGCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTAAAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACTAACGCTATATGCCGTACCTGGa                                                                                                                                                                                                                   <  1:2408896/284‑1 (MQ=255)
                                                          ccGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAAc                                                                                                                                                                                                                 <  2:1726139/289‑1 (MQ=255)
                                                              tGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGGGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAAt                                                                                                                                                                                                             <  2:2640998/289‑1 (MQ=255)
                                                                     tttgctgTTTGAAGCGCCGCAGTTGCATCGCCAGCATTTCGATCCTCGTCCGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGAATGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCa                                                                                                                                                                                                       <  1:2030078/286‑1 (MQ=255)
                                                                     ttcgctgTTGGGAGCGAGGCAGGTGCATGGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCa                                                                                                                                                                                                       <  2:36252/285‑1 (MQ=255)
                                                                     tCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCa                                                                                                                                                                                                       <  1:954887/288‑1 (MQ=255)
                                                                             ttGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACgcgc                                                                                                                                                                                                                                                                           <  1:2645988/212‑1 (MQ=255)
                                                                              tGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCCGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGCGCTGGAAGCCGAGCGGTTGATGGAAGTTAAAC‑GGGTGCTGGAGTCGGCGCGCAACGCGAAAACGGCCGGATCAACGCGCTTCTGCTGGGGCGCAGCGTGCAAGAATGCACACAAACGCGCTATGACCTAGCGCGAAAACATGAGCCAGGGGGTaga                                                                                                                                                                                              >  2:2137403/1‑286 (MQ=255)
                                                                                 aGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCg                                                                                                                                                                                          >  1:1688743/1‑289 (MQ=255)
                                                                                    gccgcagGTGCATCGCCAGCATTTCGATCCTCGTGAGGGGCAGGTCAACACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGAt                                                                                                                                                                                        <  1:1603536/285‑1 (MQ=255)
                                                                                      agcagGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCCCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGCAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGGGGAAGAATCCCCCCGAACGGGTACAGCCGTCCCTGGGATAAACGTGGCAGAGGGTAAAAGTGATggg                                                                                                                                                                                     >  2:1052858/1‑289 (MQ=255)
                                                                                             gCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAg                                                                                                                                                                              <  1:2728073/289‑1 (MQ=255)
                                                                                               aTCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGc                                                                                                                                                                            <  2:277916/289‑1 (MQ=255)
                                                                                                  gCCGGCATTGCGAGCCCCGTGGGGTGAAGGTCAGAACGTTCCATGAG‑A‑TAAGACCGGAGCTTGTCCGGAAGATTGCCACTACTGCCCGCAAAGCTGGCGCGACCAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGc                                                                                                                                                                            <  2:1261383/285‑1 (MQ=255)
                                                                                                    aaGCATTTCGGTCCTCGTCAGGTGCGGGTCAGCACGTTGC‑GGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTa                                                                                                                                                                       <  2:887492/288‑1 (MQ=255)
                                                                                                          ttCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGc                                                                                                                                                                 <  1:1977125/289‑1 (MQ=255)
                                                                                                                    gTCAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAAt                                                                                                                                                                                                             <  1:106234/235‑1 (MQ=255)
                                                                                                                      cAGGTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACa                                                                                                                                                                                                                <  1:1781874/230‑1 (MQ=255)
                                                                                                                         gTGCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATGGCAAATACTGCCCGAAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGt                                                                                                                                                   <  1:2585237/288‑1 (MQ=255)
                                                                                                                           gCAGGTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGa                                                                                                                                                 <  1:1617688/288‑1 (MQ=255)
                                                                                                                           gCAGGTAAGCACGTTGC‑TCTCGATTAAGACCGGGGATTGGCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGa                                                                                                                                                 <  2:152145/288‑1 (MQ=255)
                                                                                                                              ggTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAAtc                                                                                                                                              >  1:572906/1‑288 (MQ=255)
                                                                                                                               gTCAGCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAggggagcgc                                                                                                                                            >  2:189056/1‑281 (MQ=255)
                                                                                                                                   gCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGCAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGACCGACGCGCTTCTGTACGGGCGCGGCGTGGAAGAATCCCCCCGAACGCGCGTTGCCGTAACTGGAAACAATTGTGCAGGGCGTAAAAGCGATGGGGCTGGACGCCTCGAGTACGCTGGGCACGGTGacgcgaccccaggc                                                                                                                                        >  2:1861928/1‑276 (MQ=255)
                                                                                                                                   gCACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACaaa                                                                                                                                                                                                              <  1:623667/219‑1 (MQ=255)
                                                                                                                                    cACGTTGC‑TGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGAt                                                                                                                                                                                        >  1:2142242/1‑240 (MQ=255)
                                                                                                                                          tgctgtcgaTTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGGTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGATTCTGTATGGGCGCGGCGTGGAAGAATCCGCACGAAAGCGATAGGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAgc                                                                                                                                   >  1:235198/9‑288 (MQ=255)
                                                                                                                                          tgctgtcgaTTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGc                                                                                                                                                                            <  1:970071/239‑1 (MQ=255)
                                                                                                                                          tgctgtcgaTTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAgc                                                                                                                                   >  1:2507901/9‑288 (MQ=255)
                                                                                                                                          tgctgtcgaTTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCCCAGGCGCAgc                                                                                                                                   >  1:721034/9‑288 (MQ=255)
                                                                                                                                          gC‑TGACGATTCAGACCGGAGCTGGTCCGGAAGATTGAAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGAAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAgcgc                                                                                                                                 <  2:2376716/289‑1 (MQ=255)
                                                                                                                                                     aaGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACg                                                                                                                       <  1:298004/289‑1 (MQ=255)
                                                                                                                                                      aGACCGGAGCTTTTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGc                                                                                                                      <  1:807671/289‑1 (MQ=255)
                                                                                                                                                      aGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACg                                                                                                                       >  1:2688448/1‑288 (MQ=255)
                                                                                                                                                          cGGAGCTTGTCCGGAGAATTGCAAATACGGCCCGAAAAGCTGGCGCTAAAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCggg                                                                                                                  <  2:1688743/289‑1 (MQ=255)
                                                                                                                                                           ggAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGAGGAAGAATCCCCCCGAACGCGATCTGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGCTGGGGCCGAGGGCGCGTCTGACGTTGGGCCCGGTGAGTCAAACCCAGGCCATGCGCCCCCCGAACGCCGGGc                                                                                                                 >  2:2105866/1‑289 (MQ=255)
                                                                                                                                                                ttGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGAt                                                                                                            <  2:524291/289‑1 (MQ=255)
                                                                                                                                                                   tCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCGCAGGCGCAGCGCCGCGCGAACGGCGGGcggtagtac                                                                                                         >  2:96430/1‑281 (MQ=255)
                                                                                                                                                                       gAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGGAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCAGGTTGAGTGACTCTCAGGCGCAGAGCCTTGCGAAGGCCGGGGCGGAGtactacg                                                                                                     >  2:215488/1‑288 (MQ=255)
                                                                                                                                                                          acatGCCAACACTGCCCGCAAAGCTTGCGGTATAGAGACGGGCTGGACGCCGAGCGGTTGATGGAAGTGGAAC‑AGGTGTGGTAGTCGGCGCGCAAGGCGAGAGCTGCAGGATCGACGCGCTTCTGTATGGGTGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGGGATGGGGCGGGAGGCGTGTATGACGCTGGGAACGTTGAGCTAATCTCATGCGCGGCGCGTCGCGAACGTCGGGCTGGATTACTagaacc                                                                                                  <  2:1211502/286‑1 (MQ=255)
                                                                                                                                                                            ttGCAAATACTGCCTGAAAAGCTCGCGCTACAAAACCGGGGTGGAGGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGGGTCGGCGCGCAAAGCGGAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTacaacc                                                                                                  <  2:1599724/287‑1 (MQ=255)
                                                                                                                                                                            ttGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTacaacc                                                                                                  <  1:1891048/287‑1 (MQ=255)
                                                                                                                                                                            ttGCAAATACGGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTacaaccac                                                                                                <  1:2338464/289‑1 (MQ=255)
                                                                                                                                                                             tGCAAGTATTGCCCGGAAGGCTCGGGCTACAAAACCGGGCGGGGAGCCGGGGGGTTGATGGGAGTTGATC‑AGGTGCTGGAGACGGCGCGAAAAGCGAAGGCGGCAGGATCGACGGGCTTATGTATGGGAGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCGGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTacaaccac                                                                                                <  2:1024462/288‑1 (MQ=255)
                                                                                                                                                                             tGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAAt                                                                                                                                                                                                             <  2:92694/179‑1 (MQ=255)
                                                                                                                                                                             tGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAAt                                                                                                                                                                                                             >  1:92694/1‑179 (MQ=255)
                                                                                                                                                                                       gCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGaca                                                                                      >  2:1711981/1‑288 (MQ=255)
                                                                                                                                                                                        cccGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTggg                                                                                                                                                             >  2:2348646/1‑216 (MQ=255)
                                                                                                                                                                                        cccGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGacac                                                                                     >  2:110293/1‑288 (MQ=255)
                                                                                                                                                                                          cGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTg                                                                                                                                                                                                                                                       <  2:579865/124‑1 (MQ=255)
                                                                                                                                                                                          cGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGCTTACTACAACCAAAACCTGGACAcctc                                                                                  >  1:1881391/1‑287 (MQ=255)
                                                                                                                                                                                                gctcgcGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACAcctcgccgga                                                                            >  1:885862/6‑281 (MQ=255)
                                                                                                                                                                                                    gcGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGttt                                                                        <  1:869390/288‑1 (MQ=255)
                                                                                                                                                                                                                cGGGCTGGAGACCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGAGGCAGGATCGACGCGCTGCTGTAGGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAGAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTTTACGGCAATatc                                                            <  2:1310339/289‑1 (MQ=255)
                                                                                                                                                                                                                  ggCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGc                                                                                                                                                                            <  1:224796/175‑1 (MQ=255)
                                                                                                                                                                                                                  ggCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACg                                                                                                                       >  2:79382/1‑228 (MQ=255)
                                                                                                                                                                                                                  ggCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTg                                                                                          <  2:1793203/257‑1 (MQ=255)
                                                                                                                                                                                                                  ggCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTTTACGGCAATatca                                                           >  1:2393166/1‑288 (MQ=255)
                                                                                                                                                                                                                  ggCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGCTTTACGGCAATatca                                                           >  1:1835174/1‑288 (MQ=255)
                                                                                                                                                                                                                    cgggAAGCCGAGGGGTTGATGGAAGTTGAAC‑AGGGGCTGGAGGCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTTTACGGCAATatcatc                                                         <  2:1940949/286‑1 (MQ=255)
                                                                                                                                                                                                                    cgggAAGACGAGCGGTTGAGGGAAGTTGAAC‑AGGTGGTGGAGTCGGCACGCAAAGGGAAAGCGGCAGGATCGACGCGCTGCTGTATGGGGGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCGCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTTTACGGCAATatcatc                                                         <  2:2406050/286‑1 (MQ=255)
                                                                                                                                                                                                                    cTGGAAGCCGGGGGGTTGATGGAAGTGGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGGTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTTTACGGCAATatcatc                                                         <  2:1013695/288‑1 (MQ=255)
                                                                                                                                                                                                                       gAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTTTACGGc                                                                  <  1:2196876/276‑1 (MQ=255)
                                                                                                                                                                                                                              aGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACAACAACCTGGACACCTCGCCGGAGTTTTACGGCAAGATCATCACCACACGCa                                               >  1:206374/1‑288 (MQ=255)
                                                                                                                                                                                                                                   ttGATGGAAGGTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGa                                                                                        <  1:2665372/242‑1 (MQ=255)
                                                                                                                                                                                                                                     gATGGAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTacaacc                                                                                                  <  1:1180018/230‑1 (MQ=255)
                                                                                                                                                                                                                                        ggAAGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTTTACGGCAATATCATCACCACACGCACTTATCAGGaa                                    <  1:1711981/289‑1 (MQ=255)
                                                                                                                                                                                                                                           aGTTGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTgg                                                                                                              <  1:1431355/212‑1 (MQ=255)
                                                                                                                                                                                                                                             ttGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTaaa                                                                                                                                                                                              >  1:483158/1‑130 (MQ=255)
                                                                                                                                                                                                                                             ttGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTaaa                                                                                                                                                                                              <  2:483158/130‑1 (MQ=255)
                                                                                                                                                                                                                                             ttGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAg                                                                                                                                                                              <  2:265581/146‑1 (MQ=255)
                                                                                                                                                                                                                                             ttGAAC‑AGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACAcctcgc                                                                                <  1:52225/240‑5 (MQ=255)
                                                                                                                                                                                                                                                      gTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTTTACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACg                        >  1:761905/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                 ggcgcgcAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTTGATTACTACAACAACAACCTGGACACCTCGCCGGAGTTTTACGGCAATATCATCACCACACGCACTTACCAGGCACGCCTCGCAACGTTGGAAACAGt             >  2:250446/2‑288 (MQ=255)
                                                                                                                                                                                                                                                                 ggcgcgcAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATtacta                                                                                                       >  1:2298677/2‑198 (MQ=255)
                                                                                                                                                                                                                                                                 ggcgcgcAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATtacta                                                                                                       <  2:2298677/197‑1 (MQ=255)
                                                                                                                                                                                                                                                                 ggcgcgcAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTacaacc                                                                                                  >  1:179625/2‑203 (MQ=255)
                                                                                                                                                                                                                                                                 ggcgcgcAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGCGTTTTACGGCAATATCATCACCACACGCACTTATCAg                                       >  1:1406894/2‑262 (MQ=255)
                                                                                                                                                                                                                                                                 ggcgcgcAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGGGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCCGGCATACTACCACCACAACCTGGACACCTCGCCGGAGGTTTACGGGCATAACATCCCCACCCGCCCTTAACCGGAACGCCTCGCTACCCCGGACAAAgc             >  2:2434532/2‑287 (MQ=255)
                                                                                                                                                                                                                                                                    gcgcAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTTTACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTgcg          <  1:589683/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cgaaagcgGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTTTACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCg  <  2:1559857/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          |||                                                                                                                                                                                                                                                                               
CCCCATGGCTCACCGCCCACGCTGGACATTGAGCCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGC‑TGAGCATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCAGCCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAC‑AGGTGCTGGAGAGCGCGCGCAAAGCGAAAGCGGCAGGAAGCACGCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAGCGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCAGCCCGGAGTTTTACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCG  >  CP042583/672174‑672727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A