Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 678,037 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583678,0370AT99.0% 705.9 / NA 302intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (165/131);  minor base G (1/2);  total (169/133)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583678,0380GC99.3% 712.3 / NA 302intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (168/131);  minor base A (1/1);  total (169/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583678,0390TA99.7% 720.2 / NA 302intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (168/133);  minor base C (1/0);  total (169/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

AGAACATGGCGAGCGCGTGCGTTATCTTCACAGTGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGAGCCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTAGCTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCAAGTATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAAAGCCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGAGCCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTTGATGATGCAACACGCGCAGAATCTGGA  >  CP042583/677760‑678311
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                
    cATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTATGGATCAACTTACTGCGCGAAGGTCTGGATGTGCCGGAAGTGTCGGTGGTGGCGATCCGCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTGGTACCAGACCATTCGTCGTGCGGCACGTAACGTTCACGGTTACGGGATTCCATACGGTGACAGGATCACACCATCAATGGCGTAAGcca                                                                                                                                                                                                                                                                     >  2:884506/1‑287 (MQ=255)
                             actcagATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCACCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTga                                                                                                                                                                                                                                            >  1:2693742/6‑289 (MQ=255)
                               tcagATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTgg                                                                                                                                                                                                                                                                 >  2:2191020/4‑266 (MQ=255)
                                   atatCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGACCCAGAACATTTGTCGTGCGTGACGTAACGTTAAAGGTAAAACGATTCTCTACGGCAGTAAGATCACCCACTCAATGGCACAATCGTCTGGCGAAACTGAACGTCCGCGTGAGAAAca                                                                                                                                                                                                                                      >  2:2094038/1‑289 (MQ=255)
                                    tatCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGGGAaaaaaa                                                                                                                                                                                                                                      >  2:1630779/1‑286 (MQ=255)
                                       cGACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAAcagaag                                                                                                                                                                                                                                  <  1:2190438/289‑1 (MQ=255)
                                             cGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGACGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCATGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCACACATGGCGAAAGCGATTGGCGCAACTCAACTCAGACGTGAGAAACGGCAGaatcaa                                                                                                                                                                                                                            >  2:144437/1‑285 (MQ=255)
                                             cGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAAcag                                                                                                                                                                                                                                     >  1:564583/1‑280 (MQ=255)
                                             cGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAAcag                                                                                                                                                                                                                                     <  2:564583/280‑1 (MQ=255)
                                               tCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCg                                                                                                                                                                                                                                                  <  2:971573/265‑1 (MQ=255)
                                               tCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCg                                                                                                                                                                                                                                                  >  1:971573/1‑265 (MQ=255)
                                               tCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGtcaa                                                                                                                                                                                                                           >  1:1905846/1‑285 (MQ=255)
                                               tCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACaa                                                                                                                                                                                                                          >  1:732870/1‑289 (MQ=255)
                                               tCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACa                                                                                                                                                                                                                           >  1:1661313/1‑288 (MQ=255)
                                                        tGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAaca                                                                                                                                                                                                                 <  2:110667/289‑1 (MQ=255)
                                                         ggAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAacac                                                                                                                                                                                                                >  1:2123734/1‑289 (MQ=255)
                                                             aTTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAGCGGGAAAGCGATTCTACACGGCGAGAAGATCCCCCCATCAATGGCGAAATGGAATGGCAGAACCGAAGGTCCCCGTGGGAACAAGGAGAAACACAAGACGGAACAAGGaa                                                                                                                                                                                                            >  2:507103/1‑289 (MQ=255)
                                                                aTCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAaca                                                                                                                                                                                                                 <  2:270242/281‑1 (MQ=255)
                                                                 tCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGa                                                                                                                                                                                                                                                                           <  1:2718220/222‑1 (MQ=255)
                                                                 tCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGa                                                                                                                                                                                                                                                                           >  2:2718220/1‑222 (MQ=255)
                                                                     cgACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAAcagcag                                                                                                                                                                                                                                  <  1:952389/259‑1 (MQ=255)
                                                                     cgACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTAcgcc                                                                                                                                                                                                     >  1:643457/1‑288 (MQ=255)
                                                                     cgACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTAcgcc                                                                                                                                                                                                     >  1:28431/1‑288 (MQ=255)
                                                                               cggggtgggtTAGACGTGCTGGTGGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTg                                                                                                                                                                                           <  1:1576933/286‑1 (MQ=255)
                                                                                  ggTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATAACCCCATCAATGGCAAAAGCGATTGGCGAAACCGAACGGCGCCGTGAGAAACAGCAGAAGGACAACGAGGAACACGGAATTACGCCGCACGGCCTGACCa                                                                                                                                                                                       >  2:906357/1‑289 (MQ=255)
                                                                                        ttCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTAcgccgc                                                                                                                                                                                                   <  1:606935/271‑1 (MQ=255)
                                                                                        ttCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTAcgccgc                                                                                                                                                                                                   >  2:606935/1‑271 (MQ=255)
                                                                                        ttCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAg                                                                                                                                                                                 >  1:631665/1‑289 (MQ=255)
                                                                                            aCGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGCTTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCAGTGAGAAACAGCCGAAGTAAAACGAGGAACACGGAATTTCGCCGCAATGCCTGAACAAGAAAgggg                                                                                                                                                                              >  2:1958231/1‑288 (MQ=255)
                                                                                             cgggCTGGGTGGGATCAACTTACTGCGGGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGt                                                                                                                                                                             <  2:2280381/285‑1 (MQ=255)
                                                                                                          aTCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGgcg                                                                                                                                                                >  2:2057845/1‑288 (MQ=255)
                                                                                                           tccaCTTACTGCGGAAAGGTCTGGAAAGGCCGGAGGTGTGGCTGGTGGCGAGCGCCGACGCTGACAAAGAAGGCATCCTGCGTTCCGAGCGTTCGTTGATCAAGACCATTGATCGTGCGGAACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGAACACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGACGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGgcgc                                                                                                                                                               <  2:416883/285‑1 (MQ=255)
                                                                                                           tCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGgcgc                                                                                                                                                               <  1:2030505/288‑1 (MQ=255)
                                                                                                                 tACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCa                                                                                                                                                        <  1:2615694/289‑1 (MQ=255)
                                                                                                                  aCTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCg                                                                                                                                                                                                                                                  >  1:1971567/1‑198 (MQ=255)
                                                                                                                     gcgcGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGGACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGACATCCTGGCGCTGGGGCAGAAc                                                                                                                                                    >  1:1110948/1‑289 (MQ=255)
                                                                                                                              tCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCaa                                                                                                                                            <  1:2286276/288‑1 (MQ=255)
                                                                                                                                     atGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGc                                                                                                                                                                                              <  1:1627716/231‑1 (MQ=255)
                                                                                                                                       gCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCt                                                                                                                                                                    <  1:2553883/255‑1 (MQ=255)
                                                                                                                                       gCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCt                                                                                                                                                                    >  2:2553883/1‑255 (MQ=255)
                                                                                                                                       gCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGgcgc                                                                                                                                                               <  2:2374935/260‑1 (MQ=255)
                                                                                                                                       gCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGc                                                                                                                                                         >  2:1860073/1‑266 (MQ=255)
                                                                                                                                       gCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCg                                                                                                                                  >  2:1730707/1‑289 (MQ=255)
                                                                                                                                       gCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCg                                                                                                                                  >  1:2339053/1‑289 (MQ=255)
                                                                                                                                         cGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGaa                                                                                                                                <  1:769586/289‑1 (MQ=255)
                                                                                                                                            aagtgtcgcTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCCCCCCATCAATGGCGAAAGCGATTGGCGAAACCGACCGTCGCCGTGAGAAAAAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCGGGGGCAGAACATTACCAAAACAAAAGCGAAgg                                                                                                                              >  2:2607055/9‑288 (MQ=255)
                                                                                                                                            aagtgtcgcTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTg                                                                                                                                                                                           <  2:2416289/219‑1 (MQ=255)
                                                                                                                                            aagtgtcgcTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATAACCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGACggc                                                                                                                             >  2:2542239/9‑288 (MQ=255)
                                                                                                                                                 tcgcTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTgg                                                                                                                                                                                                                                                                 >  1:22229/4‑152 (MQ=255)
                                                                                                                                                 tcgcTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCagag                                                                                                                        >  1:456494/4‑289 (MQ=255)
                                                                                                                                                         ggCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGGGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGaaaatc                                                                                                                 >  1:1676185/1‑286 (MQ=255)
                                                                                                                                                               ccTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGgcgc                                                                                                                                                               >  2:1811973/1‑236 (MQ=255)
                                                                                                                                                               ccTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCg                                                                                                          <  1:2538316/289‑1 (MQ=255)
                                                                                                                                                                    aCGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGAttgt                                                                                                     >  2:1010498/1‑289 (MQ=255)
                                                                                                                                                                       cTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGAttgtt                                                                                                    <  2:631665/287‑1 (MQ=255)
                                                                                                                                                                        tGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCa                                                                                                                                       <  1:2376657/251‑1 (MQ=255)
                                                                                                                                                                        ggACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTGGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGa                                                                                                  <  2:85731/287‑1 (MQ=255)
                                                                                                                                                                           acaagaatGCTTGCGGCATTCCGAACATTCGTTGCTCCAGACCATTGGTCTTGCGACACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCAGCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAAGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCg                                                                                              <  2:1605062/287‑1 (MQ=255)
                                                                                                                                                                                    cTTCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAAtgt                                                                                      <  1:2433272/288‑1 (MQ=255)
                                                                                                                                                                                      tCCTGCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGcc                                                                                   <  1:714085/289‑1 (MQ=255)
                                                                                                                                                                                          gCGTTCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGaa                                                                                                                                                                                                                   >  2:1650300/1‑157 (MQ=255)
                                                                                                                                                                                             ttCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGat                                                                            <  2:456494/289‑1 (MQ=255)
                                                                                                                                                                                             ttCCGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGat                                                                            >  1:1338581/1‑289 (MQ=255)
                                                                                                                                                                                              tcggaaacgtcattGATCCATAGCATGGGCCGGGCGGCAAGCAACGTGACCGGGTGAGCGTGTATCTACGGCGATAATATCACCCCCTCAATGGCGAAAGCGATTGGCGGAACCGAACGTCTGCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGTACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGat                                                                            <  2:65740/276‑1 (MQ=255)
                                                                                                                                                                                                cGAACGTTCGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATg                                                                         <  1:1459760/289‑1 (MQ=255)
                                                                                                                                                                                                  aacgttcgttGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATgtc                                                                       >  1:1928713/9‑287 (MQ=255)
                                                                                                                                                                                                       tcgttGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGc                                                                                                <  1:2074948/256‑1 (MQ=255)
                                                                                                                                                                                                                cAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTg                                                                                                                                                                                                                                             >  1:1849673/1‑109 (MQ=255)
                                                                                                                                                                                                                  gACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTgcc                                                        >  1:1190041/1‑287 (MQ=255)
                                                                                                                                                                                                                  gACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTgca                                                        >  1:2585657/1‑288 (MQ=255)
                                                                                                                                                                                                                   aCCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATCTTCCGATGGATATTTCGCCTAAAGCGTTgcagc                                                      >  2:2134780/1‑289 (MQ=255)
                                                                                                                                                                                                                             gTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCa                                            <  1:915620/289‑1 (MQ=255)
                                                                                                                                                                                                                                cGGAACGTAACGTTAACGGTAAAGCGAGTCTCGACGGCGATAAGAGCACCCCATCAGTGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAAGTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATg                                          <  2:1190041/288‑1 (MQ=255)
                                                                                                                                                                                                                                  gCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTgg                                                                                                                                                                                                                                                                 <  2:2615983/71‑1 (MQ=255)
                                                                                                                                                                                                                                     cGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCCAAGGAAAATCGGGCACGATTGTTTAGCCGGTAAATGTGCCGGTGGAAGTGTCGCCAAAAGGGTTGAAGCCGaaacaccaggaatcgg                                    >  2:2308407/1‑276 (MQ=255)
                                                                                                                                                                                                                                     cGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGa                                                                                                  <  1:2355380/227‑1 (MQ=255)
                                                                                                                                                                                                                                     cGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTgg                                    <  1:1730707/289‑1 (MQ=255)
                                                                                                                                                                                                                                       tAACGTTAACGGTAAAGCGGTTTGCTACGGCGATACGATAACCCCATCAATGGGGAAAGCGATTGGCGAAACCGAACGTGGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGa                                   <  2:2523211/288‑1 (MQ=255)
                                                                                                                                                                                                                                        aaCGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGacg                                 >  1:1381295/1‑287 (MQ=255)
                                                                                                                                                                                                                                             tAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGa                                   >  2:408336/1‑282 (MQ=255)
                                                                                                                                                                                                                                             tAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTtg                            >  1:1258912/1‑289 (MQ=255)
                                                                                                                                                                                                                                              aaCGGTAAAGCGGTTCTCTGCGGCGATAAGATCACCCAAGAAATGGAGAAAGCGGTTGGCGAACACGAACGTCGCCGTGAGAAAAAGCAGAAGGACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTtga                           <  2:29137/289‑1 (MQ=255)
                                                                                                                                                                                                                                              aaCGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTtga                           <  1:802834/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    aaaGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGa                                                                                            <  1:1129492/218‑1 (MQ=255)
                                                                                                                                                                                                                                                    aaaGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTTGATGATGc                     >  1:517219/1‑289 (MQ=255)
                                                                                                                                                                                                                                                     aaGCGATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTTGATGATGCa                    >  1:844308/1‑289 (MQ=255)
                                                                                                                                                                                                                                                         gATTCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTTGATGATGCAacac                <  1:331791/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           ttCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCGGAAAATCCATGAGCTGGAAGGGTTTATGTGGCAACAcgc              >  2:112255/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           ttCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCGGAAAATCCATGAGCTGGAAGGGTTGATGATGCGACAcgc              <  2:2013878/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           ttCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTgg                                    <  1:2662768/267‑1 (MQ=255)
                                                                                                                                                                                                                                                           ttCTCTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGGGCCGATGGATATGTCGCCTCAAGCGTTGCATCAGAACATCCCTTAGCTGGAAGGGTTGATGATGCCACAcg               >  2:2648533/1‑288 (MQ=255)
                                                                                                                                                                                                                                                            tctcTACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTTGATGATGCAACAcgcg             <  1:1010498/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             ctctACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGGAGGGGTGATGAAGCaaacaggc             >  2:1199031/1‑282 (MQ=255)
                                                                                                                                                                                                                                                             ctctACGGCGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTTGATGATGCAACACgcgc            <  1:1412377/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              tctACGGCGAAAAGATCACCCGATGAAAGGCGAAAGCAATTGGCGAAACCGAACGTCGCCGTAAGAAACAGCAGAAGTACAACGAGGAAAACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTTGATGATGCAACACGCGCa           <  2:732870/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     cGATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGcc                                                                                   >  1:89577/1‑210 (MQ=255)
                                                                                                                                                                                                                                                                      gATAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAGTGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTTGATGATGCAACACGCGCAGAATCTg    <  1:2601638/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                       aTAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATgtcgcctaa                                                                 <  2:257383/226‑9 (MQ=255)
                                                                                                                                                                                                                                                                       aTAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTTGATGATGCAACACGCGCAGAATCTGGa  >  1:409774/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                        tAAGATCACCCCATCAATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAATCGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTgcag                                                       <  2:1371398/235‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                
AGAACATGGCGAGCGCGTGCGTTATCTTCACAGTGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGAGCCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTAGCTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCAAGTATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCAAGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAAAGCCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGAGCCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAAGGGTTGATGATGCAACACGCGCAGAATCTGGA  >  CP042583/677760‑678311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A