Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 757,431 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583757,4310AT99.7% 791.2 / NA 331intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (174/156);  minor base G (1/0);  total (175/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583757,4320GC99.4% 780.9 / NA 331intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (175/154);  minor base A (0/2);  total (175/156)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583757,4330TA99.1% 782.0 / NA 331intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (172/155);  minor base C (3/0);  total (175/156)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 331 total aligned reads displayed.

CTGGGACTTGATCCTCAAACCTGAAAATCTGGAAAAACTGAAAAGCTGCGGTGTCTCTTTCCTGGATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATAGTTCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGAGTAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCAGCTTCAGCATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAACCCAGGTATTTATCCGCCTGCGGATGTTCGTGCGAAGCTG  >  CP042583/757154‑757712
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                         
 tGGGACTTGATCCTCAAACCTGAAAATCTGGAAAAACTGAAAAGCTGCGGTGTCTCTTTCCTGGATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCga                                                                                                                                                                                                                                                                                 <  1:1659842/289‑1 (MQ=255)
     aCTTGATCCTCAAACCTGAAAATCTGGAAAAACTGAAAAGCTGCGGTGTCTCTTTCCTGGATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGCGGTCGGGCAGGCGTCCAACCGCACgaagga                                                                                                                                                                                                                                                                             >  2:2081467/1‑289 (MQ=255)
                aaaCCTGAAAATCTGGAAAAACTGAAAAGCTGCGGTGTCTCTTTCCTGGATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATg                                                                                                                                                                                                                                                                  <  1:2622955/289‑1 (MQ=255)
                 aaCCTGAAAATCTGGAAAAACTGAAAAGCTGCGGTGTCTCTTTCCTGGATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaagg                                                                                                                                                                                                                                                                              >  1:904244/1‑276 (MQ=255)
                 aaCCTGAAAATCTGGAAAAACTGAAAAGCTGCGGTGTCTCTTTCCTGGATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaagg                                                                                                                                                                                                                                                                              <  2:904244/276‑1 (MQ=255)
                       aaaaTCTGGAAAAACTGAAAAGCTGCGGTGTCTCTTTCCTGGATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGaa                                                                                                                                                                                                                                                           >  1:966599/1‑289 (MQ=255)
                                             ctccGGTGTCTCTTTCCTTGCTGCTCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaagg                                                                                                                                                                                                                                                                              <  1:1184720/245‑1 (MQ=255)
                                             cTGCGGTGTCTCTTTCCTGGATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaagaa                                                                                                                                                                                                                                                                    >  2:1471648/1‑258 (MQ=255)
                                                               ggATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCTAAGAATGGCGTGAATGTCGCGTTCTCGCGTCCAAAAGAAG‑GGGCGAGGGGGt                                                                                                                                                                                                                    >  2:2642466/1‑287 (MQ=255)
                                                                 aTGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACAGCGAGAAGGAAGCGAAGAATGGCGTGAATGTCCGGTTCTCGATTCCAAAAGAAG‑GGGCGATGGGGTGCtt                                                                                                                                                                                                                >  1:66069/1‑289 (MQ=255)
                                                                     gcCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGAt                                                                                                                                                                                                            >  1:839948/1‑289 (MQ=255)
                                                                     gcCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGAt                                                                                                                                                                                                            >  2:1961422/1‑289 (MQ=255)
                                                                     gcCAGAAGAAGTTTTTGCCACCGTGTTGAATTATCTCGGCAAAGATCACAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGCACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGTGTCGCTAACGGCTGGGCAGGTGATGTCAGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGGGAATGCCTGCTTCTCGATACCAAAAGAAG‑GGGCGATGGCGTTCTCTGag                                                                                                                                                                                                            >  2:312533/1‑288 (MQ=255)
                                                                       cAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATg                                                                                                                                                                                                           <  1:121117/288‑1 (MQ=255)
                                                                       cAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATg                                                                                                                                                                                                           <  2:713207/288‑1 (MQ=255)
                                                                          aagaagTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTAtt                                                                                                                                                                                                       >  2:1801617/1‑289 (MQ=255)
                                                                           agactggtttGCAACCGGGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGAGAAGTACACCGGACCGGCAACAGATCTGCGGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTa                                                                                                                                                                                                         <  2:2307296/279‑1 (MQ=255)
                                                                           agaagTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTa                                                                                                                                                                                                         <  1:1961422/286‑1 (MQ=255)
                                                                                   ttGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGACGTATTCGTCATg                                                                                                                                                                                                >  2:520227/1‑287 (MQ=255)
                                                                                          cGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGAt                                                                                                                                                                                       >  1:2517192/1‑289 (MQ=255)
                                                                                                      tctcGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACaaa                                                                                                                                                                           >  1:1000750/1‑289 (MQ=255)
                                                                                                      tctcGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGCCAGGCGTCAAACCGCGCGAAGGAAGCGACGAATGGCGTGAATGTCTCGGTCTCGATTCCCAAAGAAG‑GGGCCATGGCGTTCTTTGATGTATTCCCCATTCCTGGGGGTGCCAAAAACaaa                                                                                                                                                                           >  2:1705374/1‑289 (MQ=255)
                                                                                                       ctcGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGc                                                                                                                                                                                                                      <  1:220419/245‑1 (MQ=255)
                                                                                                           gCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGa                                                                                                                                                                      >  2:1750084/1‑289 (MQ=255)
                                                                                                           gCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGa                                                                                                                                                                      >  1:2318248/1‑289 (MQ=255)
                                                                                                           gCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGACGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCAAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGa                                                                                                                                                                      >  1:1640723/1‑289 (MQ=255)
                                                                                                                  tCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑At                                                                                                                                                              <  2:1014724/289‑1 (MQ=255)
                                                                                                                    ccAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCCCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCATGCGGATGCCAAAAACAAAGACGAAAcccacca                                                                                                                                                             >  2:2375242/1‑284 (MQ=255)
                                                                                                                      aaCAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACCAAGACGCAGCCT‑aatagt                                                                                                                                                          >  2:2685344/1‑284 (MQ=255)
                                                                                                                      aaCAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTAGCAAACGGCGATTTTTGCCTCGCTATCGGCTGGGCAGGTGATGTCTGTCAGGCGTCAACAAGCGCGAAGGAAGCGAAGAATGGCGTGAATTTCTCGTTCTCTCTGCCAAAAGCAG‑GGGCGATGGCGTCGTTTGATTTATTCGCCATGCCTGCGGCTCCCAAACACACAGACAACGCAT‑ATCatt                                                                                                                                                          >  2:2472090/1‑287 (MQ=255)
                                                                                                                           cACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTg                                                                                                                                                     >  2:2564161/1‑289 (MQ=255)
                                                                                                                            aCTGAAGCGGATGATGACACCGGACCGGCAACAGAGCTGCTGTTAACGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCCAGACATTAGCGACCTGGCAAACGGCGTTATTTGCGTCGCTAGCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTc                                                                                                                                                        <  2:2025484/285‑1 (MQ=255)
                                                                                                                                     gatgatTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATg                                                                                                                                                                                                                                                                  >  1:2679479/1‑172 (MQ=255)
                                                                                                                                          ttACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTgcgc                                                                                                                                      <  1:2403822/289‑1 (MQ=255)
                                                                                                                                              accggaccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCgg                                                                                                                                  <  1:365298/289‑1 (MQ=255)
                                                                                                                                                cggaccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCCCGTTCTCGATTCCAAAAGAAG‑GGGTGATGACGTTCTTTGATGTATTCGCCATGCCTACGGATGCCAAAAACAAAGACGAAACCT‑ATCAGTTCCTGAATTATCTGCTGCGCCCGGAt                                                                                                                                >  1:1315528/1‑289 (MQ=255)
                                                                                                                                                  gaccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAACCAAAGACGAAGCCT‑ATACGTTCCTGAATTACCTGCTGCGCCCGGagta                                                                                                                              >  2:48507/1‑286 (MQ=255)
                                                                                                                                                  gaccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATgt                                                                                                                              >  2:1612731/1‑289 (MQ=255)
                                                                                                                                                  gaccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATgt                                                                                                                              >  2:1773943/1‑289 (MQ=255)
                                                                                                                                                  gaccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATgt                                                                                                                              >  1:1552436/1‑289 (MQ=255)
                                                                                                                                                    ccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATgtag                                                                                                                            <  2:1198580/289‑1 (MQ=255)
                                                                                                                                                        cAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACg                                                                                                                                                                       >  1:1273865/1‑243 (MQ=255)
                                                                                                                                                               tCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGccc                                                                                                                                    <  2:1295900/270‑1 (MQ=255)
                                                                                                                                                               tCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGccc                                                                                                                                    >  1:1295900/1‑270 (MQ=255)
                                                                                                                                                                ctgctgTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTa                                                                                                                                                                                                         <  1:2472093/201‑1 (MQ=255)
                                                                                                                                                                 tgctgTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCGAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATAttt                                                                                                                >  1:2063208/1‑288 (MQ=255)
                                                                                                                                                                    tgTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCg                                                                                                             <  2:1120679/288‑1 (MQ=255)
                                                                                                                                                                     gTTAAAGCTGCGCCCGAACATTCGTTATTTCCCTTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGACTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGAc                                                                                                           <  2:2484187/289‑1 (MQ=255)
                                                                                                                                                                        aaaGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCGGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCAt                                                                                                        >  2:1834959/1‑289 (MQ=255)
                                                                                                                                                                        aaaGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCAt                                                                                                        <  1:145301/289‑1 (MQ=255)
                                                                                                                                                                        aaaGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCAt                                                                                                        <  1:820795/289‑1 (MQ=255)
                                                                                                                                                                         aaGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCAtg                                                                                                       <  1:2371009/289‑1 (MQ=255)
                                                                                                                                                                             tGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaaga                                                                                                                                                                                                                                                                     >  1:1783783/1‑129 (MQ=255)
                                                                                                                                                                              gcgcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGccc                                                                                                                                    >  2:792987/1‑255 (MQ=255)
                                                                                                                                                                              gcgcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAgcgc                                                                                                                      >  2:1030279/1‑269 (MQ=255)
                                                                                                                                                                              gcgcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATgtgt                                                                                                    >  1:2033290/1‑287 (MQ=255)
                                                                                                                                                                               cgcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCt                                                                                                 >  1:217843/1‑289 (MQ=255)
                                                                                                                                                                               cgcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCt                                                                                                 >  1:760864/1‑289 (MQ=255)
                                                                                                                                                                                gcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATg                                                                                                                               >  1:2578795/1‑258 (MQ=255)
                                                                                                                                                                                     aaCATTCGTTATTTCCATTCATCTCAATACATTAACGACCGGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCC‑AAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCaa                                                                                          <  1:1709976/289‑1 (MQ=255)
                                                                                                                                                                                       caatgtttATTTCCTTTAATCTCAAGACATTGACGACCTGGCAAACGGCGTTATTGGAGTGGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAAc                                                                                         <  2:2549926/283‑1 (MQ=255)
                                                                                                                                                                                       cATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAAc                                                                                         <  1:117144/289‑1 (MQ=255)
                                                                                                                                                                                       cATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAAc                                                                                         <  2:737150/289‑1 (MQ=255)
                                                                                                                                                                                         ttCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGACTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACg                                                                                        >  1:1512241/1‑288 (MQ=255)
                                                                                                                                                                                                      ttcatctcAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGcc                                                                                                                                                                                                   >  2:811162/5‑169 (MQ=255)
                                                                                                                                                                                                       tcatctcAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCa                                                                         <  1:1086202/286‑1 (MQ=255)
                                                                                                                                                                                                       cctcctcagaccATTAACGAGCTGGCAATCGGTGGTATTTGCTGTGCGATCGGTTGGGAAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAGGAATGGCGTGAATGTGTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGACGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCCGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCa                                                                         <  2:453185/278‑1 (MQ=255)
                                                                                                                                                                                                         atctcAATACATTAACGCCCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCAcg                                                                       <  1:1021908/288‑1 (MQ=255)
                                                                                                                                                                                                         atctcAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCAcg                                                                       <  1:1763640/288‑1 (MQ=255)
                                                                                                                                                                                                              aaTACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGcc                                                                                            >  1:165277/1‑263 (MQ=255)
                                                                                                                                                                                                              aaTACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGcc                                                                                            <  2:165277/263‑1 (MQ=255)
                                                                                                                                                                                                                 aCATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGt                                                               <  1:1612731/289‑1 (MQ=255)
                                                                                                                                                                                                                 aCATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGt                                                               <  1:640069/289‑1 (MQ=255)
                                                                                                                                                                                                                     tAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCAt                                                                                                        <  2:1897902/244‑1 (MQ=255)
                                                                                                                                                                                                                     tAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGcc                                                                                            <  1:435446/256‑1 (MQ=255)
                                                                                                                                                                                                                     tAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAAc                                                                                         >  1:805426/1‑259 (MQ=255)
                                                                                                                                                                                                                     tAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCa                                                                         <  2:686632/275‑1 (MQ=255)
                                                                                                                                                                                                                         gACCGGGCGAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCgg                                                       <  2:2063208/289‑1 (MQ=255)
                                                                                                                                                                                                                          aCCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑At                                                                                                                                                              >  1:1904921/1‑185 (MQ=255)
                                                                                                                                                                                                                          aCCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGccc                                                                                                                                    <  1:1848772/211‑1 (MQ=255)
                                                                                                                                                                                                                            cTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATgtagta                                                                                                                          <  1:1078267/219‑1 (MQ=255)
                                                                                                                                                                                                                            cTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATgtagta                                                                                                                          >  2:1078267/1‑219 (MQ=255)
                                                                                                                                                                                                                            cTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCAtg                                                                                                       >  1:2113614/1‑238 (MQ=255)
                                                                                                                                                                                                                            cTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGCTGATGTCTGGCAGGCGTCAAACCGCGCGACGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGAATCCAAAAGAAG‑GGGCGATCGTGTTCTTTGATGTATTCGCCATGCCTGCGGAGGCCAAAAACAAACACGAAGCCTAATCA‑TTCCTGAATTAACTGCTCCCCCCCGATCTATTAGTCCGTATTTCCGACCATGTATTCTATGCCAATGCCAATAGAGCAGCCACCGCCCCGGCGACTGCGGacg                                                    >  2:779542/1‑287 (MQ=255)
                                                                                                                                                                                                                               gCAAACTGCGATATTTGCGTCGCTATAGGCTGGGCAGGTGCTGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTGTCGATTCCAAAAGAAG‑GGGTGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTc                                                  <  2:1315528/288‑1 (MQ=255)
                                                                                                                                                                                                                               gCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGt                                                               <  1:711807/275‑1 (MQ=255)
                                                                                                                                                                                                                                   aCGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACctgctg                                                                                                                                         >  2:869167/1‑197 (MQ=255)
                                                                                                                                                                                                                                   aCGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACctgctg                                                                                                                                         <  1:869167/197‑1 (MQ=255)
                                                                                                                                                                                                                                         atatTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGt                                                               <  2:1225382/265‑1 (MQ=255)
                                                                                                                                                                                                                                         atatTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAAcc                                        <  2:217843/288‑1 (MQ=255)
                                                                                                                                                                                                                                          tatTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAAccc                                       <  1:2718626/288‑1 (MQ=255)
                                                                                                                                                                                                                                                   cGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAACCCAGGTATTTAt                             <  2:1552436/289‑1 (MQ=255)
                                                                                                                                                                                                                                                       atggggtGGGCAGGTGACGACTGGCAGGCATCGAGCGGGACGAAGGAGGCGCGGAATGGCTTGAATGGCTCGTTCTCGATT‑CAAAAGTAGAGGGCGCTGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGTCCCTGTGTTCTATGCCAAAGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAACCCAGGTAGTTATCCGc                         <  2:1589106/283‑1 (MQ=255)
                                                                                                                                                                                                                                                       aTCGGCTGGGCAGGTGATGTCTGGCAGGCGTAAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAACCCAGGTATTTATCCGc                         <  2:362537/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGGTAGAACCCAGGTATTTATCCGCCTTGGGATGTTCGt           >  2:2121536/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         gTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAACCCAGGTATTTATCCGCCTGCGGATGTTCGTGCGa       >  2:1347549/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                          tCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAACCCAGGTATTTATCCGCCTGCGGATGTTCGTGCGaa      >  1:47327/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                            tGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAACCCAGGTATTTATCCGCCTGCGGATGTTCGTGCGAAGc    <  1:1885286/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              gCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCa                                                                                                         <  1:507943/186‑1 (MQ=255)
                                                                                                                                                                                                                                                                              gCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAACCCAGGTATTTATCCGCCTGCGGATGTTCGTGCGaa      >  2:859314/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                         
CTGGGACTTGATCCTCAAACCTGAAAATCTGGAAAAACTGAAAAGCTGCGGTGTCTCTTTCCTGGATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATAGTTCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGAGTAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCAGCTTCAGCATTCCAAAAGAAG‑GGGCGATGGCGTTCTTTGATGTATTCGCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCT‑ATCAGTTCCTGAATTACCTGCTGCGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCAGCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAACCCAGGTATTTATCCGCCTGCGGATGTTCGTGCGAAGCTG  >  CP042583/757154‑757712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A