Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 824,545 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583824,5450AT99.1% 757.4 / NA 323intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (145/174);  minor base C (3/0);  total (149/174)
Fisher's exact test for biased strand distribution p-value = 9.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583824,5460GC99.4% 761.5 / NA 323intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (147/173);  minor base A (1/1);  total (149/174)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583824,5470CG99.7% 757.7 / NA 323intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (148/173);  total (149/174)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 323 total aligned reads displayed.

CTGGTACCGCTGGCATCCCATCTGGATGTACGTTTTGTGAGCACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTAGTCAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCAGCG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTACCCCCGTTGCAATGGAATGACAGCGG  >  CP042583/824272‑824822
                                                                                                                                                                                                                                                                                 |||                                                                                                                                                                                                                                                                                    
cTGGTACCGCTGGCATCCCATCTGGATGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGCAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGGCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGCTGCACCAGTGAAAATTTTCCTTGCGGCCTCCTCGG‑AATAACGTgtgc                                                                                                                                                                                                                                                                        >  2:949913/1‑289 (MQ=255)
      ccGCTGGCATCCCATCTGGATGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCCCCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATccc                                                                                                                                                                                                                                                                   >  1:2138001/1‑286 (MQ=255)
          ttgcttCCCATCTGGATGTACGGTTTGTGTCGACCAATTGCAATCTGGAAGTGATACTCGAAGGGGAAGATGTCAGCGGCGAAAGTCGTACTCAGGAAGTGGCGAATGCAGCGTCACAATACGCCGCATACCCACGCGTTCGTGAATCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATGGCCGATGGCCACGACATGGGAACAGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCa                                                                                                                                                                                                                                                               <  2:2637636/283‑1 (MQ=255)
          tGGCATCCCATCTGGATGTAGTGTTTGTGTCGACCAATGGCAATCTGGAAGCGGTCCTGGAAGGGGAAGATGTCCGCGGCGAAAGTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTTGAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCa                                                                                                                                                                                                                                                               <  2:905885/288‑1 (MQ=255)
          tGGCATCCCATCTGGATGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCa                                                                                                                                                                                                                                                               <  1:541582/288‑1 (MQ=255)
          tGGCATCCCATCTGGATGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCa                                                                                                                                                                                                                                                               <  1:407797/288‑1 (MQ=255)
           ggCATCCCATCTGGATGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCCAATTACCAGGTCTGATTGCCGATGGCCGCCACATGGGAACGGTGGTATTCCCTGATGCACCCGTGAAAACTTTCCTTCACCCCTCCTCGG‑AACAACGTGCGCATCGCCGCAt                                                                                                                                                                                                                                                              >  2:2591693/1‑288 (MQ=255)
             cATCCCATCTGGATGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGc                                                                                                                                                                                                                                                            >  1:1595871/1‑288 (MQ=255)
              aTCCCATCTGGATGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCt                                                                                                                                                                                                                                                           <  1:1214634/288‑1 (MQ=255)
               tCCAATCTGGGTGTCAGTTTTGTGTAGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTa                                                                                                                                                                                                                                                          <  2:1077269/288‑1 (MQ=255)
                 ccATCTGGATGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAg                                                                                                                                                                                                                                                       <  1:2532381/289‑1 (MQ=255)
                   aTCTGGATGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACCTGGGAACGGTGGTATTCGCTGGTGCTCCAGTGCAAATTTGCCTTGACGCCT‑CATCGCAAGAACGTGCGCAACGCCTACTGCTACAGtt                                                                                                                                                                                                                                                     >  2:37544/1‑289 (MQ=255)
                    tCTGGATGTACGTTTTGTGTCGACCAATGGCAATCTGGCAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGACTTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGCATTCCCTGATGCACCAGCGCACACTTTCTTTTACGCCTCCCCGG‑AAGAACGTGCTCATCGACGCCTGTTACCGttc                                                                                                                                                                                                                                                    >  2:1036706/1‑288 (MQ=255)
                        gATGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAgg                                                                                                                                                                                                                                                >  1:2414609/1‑289 (MQ=255)
                          tGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACCGTTGCAGgag                                                                                                                                                                                                                                              >  1:656899/1‑289 (MQ=255)
                          tGTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGgag                                                                                                                                                                                                                                              <  2:487159/289‑1 (MQ=255)
                           gTACGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGgaga                                                                                                                                                                                                                                             >  1:2030358/1‑289 (MQ=255)
                            tACGTTTTGTGTCGACCAATGGCAATCGGGAAGTGATCCTCGAAGGGGAAGAGGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCTCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGaa                                                                                                                                                                                                                                            <  1:885535/289‑1 (MQ=255)
                              cGTTTTGTGTCGACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAgg                                                                                                                                                                                                                                          >  1:1353530/1‑289 (MQ=255)
                                 tttgtgtcgaCCAATGGAAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCt                                                                                                                                                                                                                                       <  2:2286719/280‑1 (MQ=255)
                                  ttgtgtcgaCCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCtt                                                                                                                                                                                                                                      >  1:2618957/9‑289 (MQ=255)
                                  ttgtgtcgaCCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCtt                                                                                                                                                                                                                                      >  2:2345703/9‑289 (MQ=255)
                                   tgtgtcgaCCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCttt                                                                                                                                                                                                                                     <  2:1977809/282‑1 (MQ=255)
                                                   aaTCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAg                                                                                                                                                                                                                      <  1:1190737/288‑1 (MQ=255)
                                                              gaaccgcGAGGGGGGAGAGGTCAGTGGCGAAATTCGGACTCAGGACGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGGATTACCAGGTCTGATGGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGc                                                                                                                                                                                                           <  2:40193/282‑1 (MQ=255)
                                                              gATCCTCGAAGGGGAAGAGGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGc                                                                                                                                                                                                           <  1:1528776/288‑1 (MQ=255)
                                                              aagccTCGAAGGGGAAGCTGTCAGCGGCGCAATTCGTAGTCAGGAGTTGGCGAATGCAGCTTCACAGGCAGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCAGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGc                                                                                                                                                                                                           <  2:1775073/285‑1 (MQ=255)
                                                               aTCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTa                                                                                                                                                                                                                                                          <  2:1144029/240‑1 (MQ=255)
                                                               aTCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCg                                                                                                                                                                                                         >  1:2113609/1‑289 (MQ=255)
                                                               aTCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCCACAGTTGCCGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCg                                                                                                                                                                                                         >  1:1129839/1‑289 (MQ=255)
                                                                    cGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCgggctc                                                                                                                                                                                                    >  2:2177426/1‑284 (MQ=255)
                                                                            aaGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAAc                                                                                                                                                                                             >  1:100618/1‑288 (MQ=255)
                                                                            aaGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAAc                                                                                                                                                                                             >  1:987421/1‑288 (MQ=255)
                                                                                tGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGTTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAAcgcg                                                                                                                                                                                          <  1:37544/287‑1 (MQ=255)
                                                                                    aGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCttt                                                                                                                                                                                                                                     >  1:2081920/1‑240 (MQ=255)
                                                                                                 ggaCTCAGGAAGTGGCCAACGCAGCGTCACAAGTCGCGGCATTCCGACTCGTCGGTGAAGCATGATTGCGTCGCCAACGAGAGTTTCGCGAATTACCAGGTCTGGTTGCCGATGGCCGCGACATGGGAACGGTTGTATTCACTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAAGGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCg                                                                                                                                                                       <  2:2031256/287‑1 (MQ=255)
                                                                                                          aaGTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAg                                                                                                                                                               <  1:1485079/288‑1 (MQ=255)
                                                                                                            gTGGCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCACCGCGCGTTTCGCGAATTACCAGGTCTGATTGCCCATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATCCTCCAGTTGCGGGCGAAGGGCTTTAGAGCTAACTTTGAGCGCCTTTTGGCCCAGATCCAACAACCCGCCGCCCGCCCACGTAACCGCGCCGTAgcc                                                                                                                                                             >  2:2139565/1‑287 (MQ=255)
                                                                                                               gCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCa                                                                                                                                                          >  2:1544678/1‑288 (MQ=255)
                                                                                                               gCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGAGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCa                                                                                                                                                          >  2:532722/1‑288 (MQ=255)
                                                                                                               gCGAATGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCA‑TATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTTCGCATCGCCGCATGCTACAGTTGCCGGAGAAGGGCTTTAGTGTTAACTTTGAGTGCCTTTTGGCCGAGTTCAAAGCACGCGACGACCCCGATCGTAACCGACCGGTATCGcc                                                                                                                                                           >  2:1596155/1‑286 (MQ=255)
                                                                                                                   aTGCAGCTTCACAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGt                                                                                                                                                     >  2:1356394/1‑289 (MQ=255)
                                                                                                                            cacAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGc                                                                                                                                            <  1:563510/287‑1 (MQ=255)
                                                                                                                                 gTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGc                                                                                                                                                                                                           <  2:475819/221‑1 (MQ=255)
                                                                                                                                 gTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGAt                                                                                                                                        >  1:2496934/1‑288 (MQ=255)
                                                                                                                                   cgcgGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGc                                                                                                                                                                                                           >  2:1263864/1‑219 (MQ=255)
                                                                                                                                   cgcgGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATg                                                                                                                                       <  1:1035485/287‑1 (MQ=255)
                                                                                                                                           tCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAGCGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGc                                                                                                                                                                                                           >  1:1584223/1‑211 (MQ=255)
                                                                                                                                           tCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAGCGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGc                                                                                                                                                                                                           <  2:1584223/211‑1 (MQ=255)
                                                                                                                                            cccACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCgg                                                                                                                                                <  2:1513384/269‑1 (MQ=255)
                                                                                                                                            cccACGCGTTCGTGAAGCATTATGGCGCCGCCAACGGGCGTTTGGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTg                                                                                                                            <  2:783210/289‑1 (MQ=255)
                                                                                                                                                   gTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTcca                                                                                                                     <  1:1645868/289‑1 (MQ=255)
                                                                                                                                                   gTTCGTGAAGCATTATTGCGTCGCAAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTcca                                                                                                                     <  1:2171403/289‑1 (MQ=255)
                                                                                                                                                    ttCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCCCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTACCTTTGAGCTCCTTTTGGCCGAGATCCAAGAACGACACGAACGCGATCGTAAACGATCGGTAGCGACACTGGCTCCGGCAGCCGATTCATTAGTGGTGGATTccc                                                                                                                     >  2:1848486/1‑287 (MQ=255)
                                                                                                                                                     tCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTccacc                                                                                                                   <  1:942347/289‑1 (MQ=255)
                                                                                                                                                     tCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTccacc                                                                                                                   <  1:1302268/289‑1 (MQ=255)
                                                                                                                                                     tCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTccacc                                                                                                                   <  1:1823794/289‑1 (MQ=255)
                                                                                                                                                     tCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTccac                                                                                                                    <  1:1869540/288‑1 (MQ=255)
                                                                                                                                                     tCGGGAAGCATTATTGGTTCGCCACCGCGGGTTTGGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTACCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTccacc                                                                                                                   <  2:1205677/289‑1 (MQ=255)
                                                                                                                                                      cGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCg                                                                                                                                          <  1:2353879/265‑1 (MQ=255)
                                                                                                                                                      cGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCg                                                                                                                                          >  2:2353879/1‑265 (MQ=255)
                                                                                                                                                      cGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTccacc                                                                                                                   >  2:592169/1‑288 (MQ=255)
                                                                                                                                                      cGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCCACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGGTCAAAGAACGCGACGACCGCGATCGTAACCGAACAGTACTCCCACGGGTTCCGGCAGCCGATGCTTTAGTGTTAGCTTccacc                                                                                                                   >  2:1377339/1‑288 (MQ=255)
                                                                                                                                                               ttattGGGTCGCCAACGCGCGTTTCGCAAATTACCAGGTCGGATTGCCGATGGCCGGGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCa                                                                                                         <  2:100618/289‑1 (MQ=255)
                                                                                                                                                                  ttGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTg                                                                                                      >  1:388333/1‑289 (MQ=255)
                                                                                                                                                                       tCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTcca                                                                                                                     >  1:143647/1‑269 (MQ=255)
                                                                                                                                                                          ccAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGc                                                                                                                                                                                                           >  2:1889612/1‑180 (MQ=255)
                                                                                                                                                                                 gcgTTTCGCGAATTAACAGGTCTGATTGCCGATGGCGGAGACATGGGAACGGTGGTATTCCCTTATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGaaa                                                                                       <  2:331156/289‑1 (MQ=255)
                                                                                                                                                                                  cgTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGaaa                                                                                       <  2:248495/288‑1 (MQ=255)
                                                                                                                                                                                                         aTTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCa                                                                                                         <  1:609149/247‑1 (MQ=255)
                                                                                                                                                                                                         aTTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGa                                                               >  2:739186/1‑289 (MQ=255)
                                                                                                                                                                                                         aTTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGa                                                               >  1:2247946/1‑289 (MQ=255)
                                                                                                                                                                                                            gCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAAt                                                            <  1:808636/289‑1 (MQ=255)
                                                                                                                                                                                                               gATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGa                                                                                                                                         <  2:2223988/209‑1 (MQ=255)
                                                                                                                                                                                                                 tGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGcgac                                                                                                                                                                                        <  2:830498/160‑1 (MQ=255)
                                                                                                                                                                                                                       cgACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCtt                                                                                                              <  2:689950/228‑1 (MQ=255)
                                                                                                                                                                                                                              ggAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGct                                                       >  1:2680375/1‑276 (MQ=255)
                                                                                                                                                                                                                                aaCGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGAc                                         <  1:2314535/288‑1 (MQ=255)
                                                                                                                                                                                                                                    gtggtATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCGTCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTcca                                                                                                                     >  2:1312989/1‑208 (MQ=255)
                                                                                                                                                                                                                                    gtggtATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCGTCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTcca                                                                                                                     <  1:1312989/208‑1 (MQ=255)
                                                                                                                                                                                                                                    gtggtATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAAt                                    >  2:282337/1‑289 (MQ=255)
                                                                                                                                                                                                                                    gtggtATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTAAAATACGGGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAAt                                    >  1:2383459/1‑289 (MQ=255)
                                                                                                                                                                                                                                        tATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCtt                                                                                                                                    <  1:466821/189‑1 (MQ=255)
                                                                                                                                                                                                                                        tATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCAGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGc                                >  1:511977/1‑289 (MQ=255)
                                                                                                                                                                                                                                          ttCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGgaga                                                                                                                                                                                                                                             <  1:692205/82‑1 (MQ=255)
                                                                                                                                                                                                                                           tCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGt                             <  1:282337/289‑1 (MQ=255)
                                                                                                                                                                                                                                               tGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGTCACAATACGCGCGCCACAAATTGGCTCTCGCATAAGCGACCCGAtttcaatttcc                          >  2:2716213/1‑280 (MQ=255)
                                                                                                                                                                                                                                               tGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTAcc                          >  2:253008/1‑288 (MQ=255)
                                                                                                                                                                                                                                               tGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTAcc                          >  2:1436183/1‑288 (MQ=255)
                                                                                                                                                                                                                                               tGATGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCGTTAGGGTTGGATTCCACCACCCTAAGCATTGAGCAAGGGATTGAAAAGACGCTAAAATACGCGCGCCAGAAATTGGCCTCCGCATAATCGACCGAAATTGCAATAcc                          >  2:431815/1‑288 (MQ=255)
                                                                                                                                                                                                                                                 aTGCACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTcca                                                                                                                     <  2:619784/195‑1 (MQ=255)
                                                                                                                                                                                                                                                    cACCAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTcca                                                                                                                     <  1:1935296/192‑1 (MQ=255)
                                                                                                                                                                                                                                                       cAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTACCCCCGTTGCa                 >  2:2404271/1‑289 (MQ=255)
                                                                                                                                                                                                                                                       cAGTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTACCCCCGTTGCa                 >  1:1692776/1‑289 (MQ=255)
                                                                                                                                                                                                                                                         gTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTCTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTACCCCCGTTGc                  <  1:1746668/286‑1 (MQ=255)
                                                                                                                                                                                                                                                         gTGAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTCTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTACCCCCGTTGc                  >  2:1746668/1‑286 (MQ=255)
                                                                                                                                                                                                                                                           gAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTACCCCCGTTGCaa                <  1:781802/286‑1 (MQ=255)
                                                                                                                                                                                                                                                           gAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTACCCCCGTTGCaa                <  1:342899/286‑1 (MQ=255)
                                                                                                                                                                                                                                                           gAAAATTTTCCTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACAACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTACCCCCGTTGCaa                <  1:1567681/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ccTTGACGCCTCCTCGG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGACTGAAAAAGCGCTACAATACGCGCGCCAGAAACTGGCTCTCGCATAAGCGACCGAATTTGCAGTACCCCCGTTGCAATCGCATGACTGc    >  2:1660629/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                 |||                                                                                                                                                                                                                                                                                    
CTGGTACCGCTGGCATCCCATCTGGATGTACGTTTTGTGAGCACCAATGGCAATCTGGAAGTGATCCTCGAAGGGGAAGATGTCAGCGGCGAAATTCGTACTCAGGAAGTGGCGAATGCAGCTAGTCAAGTCGCGGCATTCCCACGCGTTCGTGAAGCATTATTGCGTCGCCAACGCGCGTTTCGCGAATTACCAGGTCTGATTGCCGATGGCCGCGACATGGGAACGGTGGTATTCCCTGATGCACCAGTGAAAATTTTCCTTGACGCCTCCAGCG‑AAGAACGTGCGCATCGCCGCATGCTACAGTTGCAGGAGAAGGGCTTTAGTGTTAACTTTGAGCGCCTTTTGGCCGAGATCAAAGAACGCGACGACCGCGATCGTAACCGAGCGGTAGCGCCACTGGTTCCGGCAGCCGATGCTTTAGTGTTGGATTCCACCACCTTAAGCATTGAGCAAGTGATTGAAAAAGCGCTACAATACGCGCGCCAGAAATTGGCTCTCGCATAAGCGACCGAATTTGCAGTACCCCCGTTGCAATGGAATGACAGCGG  >  CP042583/824272‑824822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A