Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A24 F184 I1 R1	14	40.6	1471776	90.4%	1330485	142.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	187,969	C→A	D228Y (GAT→TAT)	glnD ←	uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	187,969	0	C	A	100.0%	72.8 / NA	27	D228Y (GAT→TAT)	glnD	uridylyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (13/14); total (13/14)

ATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGTTCTGTTCTTCAACTTTCGCCGCGTAGAACTTGTCGGAAGGCCAGAATCCTTCGCTGAA  >  NC_000913/187836‑188104

aTACGCCACAATATATGCAGACATTCGTTTAATTCAGCCCGCTCCGCTGGGGTTAAGAAGCCAAACCCAACCATATCATCCAGCGAGGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTCGATATAGCGCAAgcc                                                                                                                                >  2:533069/1‑143 (MQ=255)

      cacaATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAgg                                                                                                                          >  1:103295/1‑143 (MQ=255)

               tGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCtttt                                                                                                                 >  2:18796/1‑143 (MQ=255)

                gCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGGGTGGATCTAGCGCAAGCCGCCAGGGCTGCCTTTg                                                                                                                >  2:180403/1‑143 (MQ=255)

                  aGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTAGAt                                                                                                              >  2:414520/1‑143 (MQ=255)

                       ttCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAAGGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGCTGTCTg                                                                                                         <  1:447695/143‑1 (MQ=255)

                        tCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTgg                                                                                                        >  1:640878/1‑143 (MQ=255)

                              aaTTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAg                                                                                                  <  1:558012/143‑1 (MQ=255)

                                ttCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGt                                                                                                >  1:321077/1‑143 (MQ=255)

                                      ccgctccgctGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGc                                                                                          <  1:518615/143‑1 (MQ=255)

                                        gctccgctGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACGCATTGCAGAGTGTGGATATAGCGCAAGGCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTg                                                                                        >  2:625177/1‑143 (MQ=255)

                                        gctccgctGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTg                                                                                        <  1:349858/143‑1 (MQ=255)

                                                      gagaagCCAAACCCGACCACTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACg                                                                          <  2:578717/142‑1 (MQ=255)

                                                            ccAAACCCGGCCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATg                                                                    <  2:570026/143‑1 (MQ=255)

                                                                   cGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGTATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTgg                                                             >  1:689701/1‑143 (MQ=255)

                                                                         tttCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGgttctgt                                                       >  1:297292/1‑143 (MQ=255)

                                                                         tttCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGgttctgt                                                       >  1:245802/1‑143 (MQ=255)

                                                                             aTCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCGGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGTTCTGttctt                                                   <  1:57197/143‑1 (MQ=255)

                                                                               ccAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGTTCTGTTCTTCa                                                 <  2:103295/143‑1 (MQ=255)

                                                                                    tgcgTTGCGGCAAAGTCGCGGCGGGCCCCCCATTGCACAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTTGTGCCATGGTAACGCTGATGGCGCTGGTTCTGTTCTTCAACttt                                            <  2:628337/140‑1 (MQ=255)

                                                                                                acaTGACGGCGGGCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGTTCTGTTCTTCAACTTTCGCCGCGTAGaa                                <  2:656648/141‑1 (MQ=255)

                                                                                                    gACGGGGGGCCACCCATTGCGGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAACATCAACAATGATTTTTCATTAACGCTGATGGCGCTGGTTCTGTTCTTCAACTTTCGCCGCGTAGAACTTg                            <  2:519708/143‑1 (MQ=255)

                                                                                                          gggCCACCCATTGCAGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCCGGTTCTGTTCTTCAACTTTCGCCGCGTAGAACTTGTCTGaa                      <  2:640878/143‑1 (MQ=255)

                                                                                                                  ctttGCTGAGTGTGGATATAGCGCAAGCCGCCAGGGCTGCTTCTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGTTCTGTTCTTCAACTTTCGCCGCGTAGAACTTGTCGGAAGGCCAGaa              <  1:58494/141‑1 (MQ=255)

                                                                                                                        agagTGTGGATATAGCGCAAGCCGCCAGGGCTGCTGTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGTTCTGTTCTTCAACTTTCGCCGCGTAGATCTTGTCGGAAGGCCAGAATCCTTc        <  2:249672/143‑1 (MQ=255)

                                                                                                                              tgGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGTTCTGTTCTTCAACTTTCGCCGCGTAGAACTTGTCGGCAGGCCAGAATCCTTCGCTGac  >  1:554947/1‑142 (MQ=255)

                                                                                                                              tgGATATAGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGATACGTACGACTGGGTTCGCCGCGTAGAACTTGTCGGAAGGCCAGAATCCTTCGCTGaa  >  1:165365/1‑143 (MQ=255)

ATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGTTCTGTTCTTCAACTTTCGCCGCGTAGAACTTGTCGGAAGGCCAGAATCCTTCGCTGAA  >  NC_000913/187836‑188104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A