Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A24 F184 I1 R1 14 40.6 1471776 90.4% 1330485 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)46 (1.140) 41/282 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 1293032 =0 (0.000)51 (1.270) 42/282 0.0 100% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)noncoding (1/768 nt) IS1 repeat region

AATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/258036‑257908
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATT  <  NC_000913/1293039‑1292902
                                                                                                                                                                                                                                                                            
AATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAA                                                                                                                              <  1:79060/143‑1
        GCAGCACCTTGCACTGCTGGGACGGAAGTCGCTGCCGTTCTCAAAATCGGTGGACCTTCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCCATAAGTTGTAGTCAT‑TACCCGGCACAAAATACAGAACAATT                                                                                                                      <  1:519116/143‑1
        GCAGCACCTGGCACGGCTGGGACGGAAGGCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATT                                                                                                                      <  1:81880/143‑1
                  GCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATACAACACCAGCAATAAGTTGGCG‑CATCCATTCTTCACACAATAAAGAACAATTTTGAATTCCT                                                                                                            >  2:150114/1‑143
                             ACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATCAAGAACAATTTTGAATTCCTTACATTCCTGG                                                                                                 >  2:295453/1‑143
                             ACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGG                                                                                                 >  1:676348/1‑143
                             ACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGG                                                                                                 <  2:610428/143‑1
                                GAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTA                                                                                              >  2:173176/1‑143
                                 AAGTCGCTGTCGTTCTCAAAATCGGTAGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTAT                                                                                             <  2:646321/143‑1
                                  AGTCGCTGTCGTTGTCAAAATCGGGGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATT                                                                                            <  2:558168/143‑1
                                   GTCGGTGTCGGGCTCAAAATCGGTGGAGCTGCATAACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTG                                                                                           <  2:665927/143‑1
                                      GCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCAC                                                                                        >  1:440104/1‑143
                                          TCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCATGGCTATTGCACAACT                                                                                    >  1:96424/1‑143
                                            GTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGA                                                                                  >  1:393897/1‑143
                                             ggCTCAAAGTCGGTGGAGCTGCATGACAAAGTCAGCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAA                                                                                 <  1:454160/141‑1
                                             TTCTCAAAACCGGAGGATCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGGCGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAA                                                                                 <  1:454095/143‑1
                                              cctaAAAATCGGTCGAGCTGCAGGACAAAGTCATCGGGCATTATCTTAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAAT                                                                                <  2:560239/139‑1
                                                TCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAAAAATTTTGAATTCCTTACATTACTGGCTATTGCACAACTGAATTT                                                                              >  2:80541/1‑143
                                                TCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTAGAAGTCAT‑CACCCGGCACAAAATAAAGAAGAATTTTGAATTCCTTACATTCCTGAATATCGCACAAATGAAact                                                                              >  2:98405/1‑140
                                                 CAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTGGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTca                                                                             >  1:74588/1‑141
                                                  AAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAA                                                                            >  2:688615/1‑143
                                                     ATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAAGAAGTTGGCGTCAT‑TACCCGGCACAAAATAAAGAGCAATTTTGAATTCCTTACCTTCCTGGCTGTTGCACACCTGACTTTAAGGC                                                                         >  1:150822/1‑143
                                                           GGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATT                                                                   >  1:214780/1‑143
                                                             AGCGGCAGGACAAAGTCATCGGGCATTAGCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTAT                                                                 <  1:139876/143‑1
                                                                TGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAACAAGGCACTGG‑TGCCCGTCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTAC                                                              <  1:166323/143‑1
                                                                       CAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAGCTGAATTTAAGGCTCTATTATTACCTCAACA                                                       <  1:517938/143‑1
                                                                        AAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAA                                                      >  1:290303/1‑143
                                                                               TCGCGCATTATCTGAACATCGAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCC                                               <  2:98177/143‑1
                                                                                  GGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCCACAAACCACCCCAA                                            >  2:187389/1‑143
                                                                                          CTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAAATGAATCATTATCTCTATTATTACCTCAACAAACCACCCCAATAAAAGTT                                    >  2:710775/1‑143
                                                                                           TGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTT                                   <  1:210636/143‑1
                                                                                            GAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTG                                  <  1:112021/143‑1
                                                                                             AACATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGA                                 <  2:2109/143‑1
                                                                                               CATAAAACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAGGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGA                               >  1:701385/1‑143
                                                                                                    AACACTATCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACT                          >  2:348473/1‑143
                                                                                                           TCAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCAAATATAAGTTTGAGATTACTACAATGA                   >  2:129115/1‑143
                                                                                                            CAATAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAG                  <  2:293696/143‑1
                                                                                                               TAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGA               >  1:561460/1‑143
                                                                                                               TAAGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATGACTACAATGAGCGA               >  1:113323/1‑143
                                                                                                                 AGTTGGAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAG             <  2:96424/143‑1
                                                                                                                  GTTGGAGTCAT‑TACCCGGCACATAATAAAGAACTGTTTTGTTTCCCCAACATACCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGC            <  1:530527/143‑1
                                                                                                                     GGAGTCAT‑TACCCGGCACACAATAAAGAACAATTTTGAATTCCTTACATCCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACT         <  1:269864/143‑1
                                                                                                                      GAGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGAACAACTGAATTTAAGGCACTATTATTACCTCAACAAACCACCCCAATATAAGCTTGAGATAACTACAATGAGCAAAGCACca        >  2:238289/1‑141
                                                                                                                       AGTCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTA       <  2:440104/143‑1
                                                                                                                         TCAT‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCCCACCTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATTAGCGAACCACTTAAA     >  1:83314/1‑143
                                                                                                                            T‑TACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATT  <  1:262809/143‑1
                                                                                                                                                                                                                                                                            
AATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCAT‑TACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/258036‑257908
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATT  <  NC_000913/1293039‑1292902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A