Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A24 F184 I2 R1	15	56.1	2030262	91.3%	1853629	141.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,968,509	Δ1 bp	coding (594/1104 nt)	wecA →	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,968,509	0	A	.	95.5%	177.4 / 0.9	45	coding (594/1104 nt)	wecA	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (17/25); total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCAAAACCCATCCCATCAGCC  >  NC_000913/3968375‑3968645

aTGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGGCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATg                                                                                                                                 <  1:623718/143‑1 (MQ=255)

 tggtggATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGTAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGc                                                                                                                                <  1:62939/143‑1 (MQ=255)

 tGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGc                                                                                                                                >  1:721499/1‑143 (MQ=255)

   gTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCtt                                                                                                                              >  1:311588/1‑143 (MQ=255)

    ttGATGGCAGTGATGGCTTGTTGGGTGGGTTGTCCTGCGACTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTa                                                                                                                             <  1:690394/143‑1 (MQ=255)

         gtcATTGATGGCTTGCTGGGGGGGTTGTCCTGCGTCGAGGTAGCAGCAATCGGTATGATTGTGTGGTCCAACTTGCAAACCGGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCtt                                                                                                                        <  1:234966/141‑1 (MQ=255)

         ggCAGTGATGGCGTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCtt                                                                                                                        <  2:48761/143‑1 (MQ=255)

            aTTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAACAGGGACGATTTTGTGGTTCGGCGGGCAAACCAGCCTCGCAAGCTGGTGCTTTGCGCAGCTCGCCGCCGTCATG‑CACCCATCATCGTTAACCGTggg                                                                                                                     >  2:848755/1‑142 (MQ=255)

                  ggCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCCGCAATCGGTCTGCTTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCGTTGCGATGATCGCCGCCATCCT‑CCCTACATCACGCGTGACCTCGGTACCCTg                                                                                                               >  1:327155/1‑143 (MQ=255)

                   gCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTgg                                                                                                              <  2:626465/143‑1 (MQ=255)

                          ggggggGTTGTCGGGCGCCTCGTCTGGAGCAATGGGTATGATTTTGTGGGTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTcgccg                                                                                                       <  2:609794/143‑1 (MQ=255)

                              gggTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCACTCTTAACCTTCGTCTCCTGGGTCGCCGCCAc                                                                                                   >  1:411280/1‑143 (MQ=255)

                                 ttGTCCTGCGTCTAGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACaaa                                                                                                <  1:161755/143‑1 (MQ=255)

                                 ttGTCCTGCGTCGCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACaaa                                                                                                <  1:46667/143‑1 (MQ=255)

                                 ttGTCCGGCGTCTAGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCGTGGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACaaa                                                                                                <  1:166509/143‑1 (MQ=255)

                                     ccTGCGGCTCGTTTGCCGCCAGCCGGATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCt                                                                                            <  2:397597/143‑1 (MQ=255)

                                        gCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTa                                                                                         >  2:963944/1‑143 (MQ=255)

                                         cGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGAGGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGCATCCTGGGTCGCCGCTACAAACGCTTTAt                                                                                        >  1:328448/1‑143 (MQ=255)

                                          gTCTCGTTTGCACCAATCGGGATGATTTTGGCGTGCGTTGGGCAAACCGTCCTCGCGATCTGGTGCTTAGAGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTAGCCTGGGTCGCCGCTACAAAGTCTTTATg                                                                                       <  2:247862/143‑1 (MQ=255)

                                                    cagcaATCGGTATGACTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCgg                                                                             <  1:765814/143‑1 (MQ=255)

                                                     agcaATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGAGATGCggg                                                                            >  1:831314/1‑143 (MQ=255)

                                                     agcaATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCGCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCggg                                                                            <  1:161082/143‑1 (MQ=255)

                                                         aTCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTTACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGt                                                                        <  1:828816/143‑1 (MQ=255)

                                                                tGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAACGTCTTTATGGGTGATGCGGGCAGTACGCTgc                                                                 >  1:538532/1‑142 (MQ=255)

                                                                tGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGa                                                                 >  1:730695/1‑143 (MQ=255)

                                                                           ttCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTAcc                                                      <  1:848932/143‑1 (MQ=255)

                                                                               aCGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGAGGGCAGTACGCTGACTGGGGTTACCGttc                                                  >  2:699563/1‑142 (MQ=255)

                                                                                cGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTTGGTTGCCGCTACAAAGTCTTTATGTGGGATGCGGGTATTACGCTGATTGGTTTTACCGTTAt                                                 <  1:555017/143‑1 (MQ=255)

                                                                                   gCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTg                                              >  1:183499/1‑143 (MQ=255)

                                                                                      aaCCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGAt                                           <  1:514794/143‑1 (MQ=255)

                                                                                             gtCGCTATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTc                                    <  2:171075/142‑1 (MQ=255)

                                                                                             cTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTc                                    <  2:183499/143‑1 (MQ=255)

                                                                                              tCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGCTCCTGCTCg                                   >  1:302105/1‑143 (MQ=255)

                                                                                                          gCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAgg                       <  1:568537/143‑1 (MQ=255)

                                                                                                           cTTTGCGATGATCGCCGCCATCCTGCC‑GACACGAGGCATAAGCGTGGGAACGTGGGTCGCCGCTACGAAGTGTTTATGGGGGAGGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAggg                      <  1:228858/143‑1 (MQ=255)

                                                                                                            tttGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTCCCGTTATCTGGCTCCTTCTCGTAATGAccccgtgc                     >  1:41321/1‑138 (MQ=255)

                                                                                                                cgctgatCGCGGCCGTCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTGTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCaaaa                 <  2:387908/140‑1 (MQ=255)

                                                                                                                cGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGGTGCGGGCCGTACGCTGATTGGTTTTCCCGTTATCTGGATCCTGCCCGAAACGACCCAGCGCaaaa                 >  1:564100/1‑143 (MQ=255)

                                                                                                                cGATGATCGCCGCCATCCTG‑CAGACTTCATGCTGAACCGGTGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCaaaa                 <  1:375019/143‑1 (MQ=255)

                                                                                                                  atgatCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTCATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGTTCCTGCTCGAAACGACCCAGAGCCAAAcc               >  2:785890/1‑143 (MQ=255)

                                                                                                                  atgatCGCCGCCATCCTGCC‑TACATCATGCTTAACATTCATCGACTACCAAGCGCCTACTAAGTCTGTATGGGTGATGCGGGCAGTACGCCGACGGGTAGCAGCGTTATCTGGATCCTGCTCGAAACGACACAGGGCAAAAcc               >  2:662708/1‑143 (MQ=255)

                                                                                                                      tCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGGATCCTGGGTCCCCGCTACAAAGTCTTTATGTGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTCCTCGAAACGACCCAGGGCAAAAcccatc           <  1:662708/143‑1 (MQ=255)

                                                                                                                       cgccgcCATCCTGCC‑TACCTCATGCTTAACCTTGGCATCCTGTGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCAAAAcccatcc          <  1:53399/143‑1 (MQ=255)

                                                                                                                            ccATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTGTTACCGTTATCTGGATCATGCTCGAAGCGACCCAGCGCCAAACCCATCCCATCa     >  2:909629/1‑143 (MQ=255)

                                                                                                                               tCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATTCGGGCAGTACGCTGCTTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCCCGACCACTCCCATCAGca  >  2:816139/1‑142 (MQ=255)

ATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCAAAACCCATCCCATCAGCC  >  NC_000913/3968375‑3968645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A