Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F9 I0 R1	75	124.0	2465218	96.8%	2386331	230.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	729,695	T→G	23.4%	V38G (GTG→GGG)	rhsC →	Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	729,695	0	T	G	23.4%	86.4 / 3.7	54	V38G (GTG→GGG)	rhsC	Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand): ref base T (17/22); new base G (11/3); total (29/25)
Fisher's exact test for biased strand distribution p-value = 3.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGC  >  NC_000913/729475‑729933

tttCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGGGAc                                                                                                                                                                                                                                >  1:1152561/1‑239 (MQ=255)

 ttCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCCGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACg                                                                                                                                                                                                                               >  1:703702/1‑239 (MQ=255)

       aTTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGGTCAGCCGGGGGACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGGGTGCCCCGGCGGGGGGGCGGCCgg                                                                                                                                                                                                                          >  1:323673/1‑238 (MQ=255)

                aTCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGGGGCGTCCGGCCCTCCGGTc                                                                                                                                                                                                                >  1:199540/1‑239 (MQ=255)

                aTCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGAATTGTCCAGGGGTCAGCCGGGGTACGCATCGGTGCCCCCCCCGGCGTGGCCTGGTCGGGGTGGCCCGGGGGGGGGGCGGCCGGCCATCCgggc                                                                                                                                                                                                                >  2:1065437/1‑237 (MQ=14)

                     aCAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGGTCCGGCGGGGGACGCATCGGTGCCCCCCCCCGCGTGGCCCGTTCGGGGGGCCCCGGCGGGGGGGCGGCCCGCCCTcccggccctt                                                                                                                                                                                                            >  2:124016/1‑230 (MQ=14)

                     aCAGATATCTCACTTAAGGCTTTCTTATAAATCTGCAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGTTGAGCGGAAAACCGGCGACGCGTCAGGGCGACATGCCGCAGTATCGCGG‑AGCATTGTCAAGGGTTCAGCGGGGGTACACCCCGGTGCCCCCACCGGCGTGGCCCGTTCGGTCGGCCCCCGCGGGGGGGCGCCCCGCCCTCCGGTCTAT‑cc                                                                                                                                                                                                          >  2:582675/1‑238 (MQ=14)

                                   tAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCCGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGGGGCCCGTTCGGGGTGCCCCGGCGGGGGGGCGTCCGGCCCTCCCGGCAAT‑CCGCCGCTCGGGGCaa                                                                                                                                                                                            >  1:429386/1‑239 (MQ=14)

                                   tAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCaa                                                                                                                                                                                            <  2:1152561/239‑1 (MQ=255)

                                    aaGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCATTGTCCAGGGTTCAGCCGGGGGACGCCTCGGGGCCCCCCCCGGCGTGGCCTTTTCGGGGTGCCCCGCCGGGGTGACGTCCGGCCATCCGGGCAAT‑CCGCTGCCCGGTGCaaa                                                                                                                                                                                           >  1:322692/1‑239 (MQ=14)

                                      ggCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGGTTAGCCGGGGAACGCATCGGGGCCCCCACCGGCGTGGACTGTTCGGGGTGGCCCGGCGGGGGGGAGGCCCGCCATCCCGTCCAT‑CCCCCTCTCgggggcaagg                                                                                                                                                                                         >  1:1164467/1‑231 (MQ=14)

                                          ttCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGCGGCCTGTTCGGTGGGGCCCCGCGGGGGGGCGTCCGGCCCTCCCGCCCAA‑TCGCTGCCCGGTGCaaacggc                                                                                                                                                                                       >  1:517226/1‑233 (MQ=14)

                                          ttCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGGACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGGGCCCCGGCGGGGGGGCGGCCGGCCATCCCGGCAAT‑CCGCCGCCCCGTGCCAAGGGCCt                                                                                                                                                                                     >  1:275946/1‑239 (MQ=14)

                                           tCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCtt                                                                                                                                                                                    <  1:1065437/239‑1 (MQ=255)

                                                      tGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCATTGTCCCGGGTTCAGCCGGGGGCGCCACCGGGGCCCCCCCCGGCGGGGCCCTTTCGTGGTGCCCCTGGGGGGGGACGCCCCCCCCCCCGGGCCAT‑CCCCGCGCCGGGGCACAGGGCCTTccccggggaa                                                                                                                                                                          >  2:820634/1‑231 (MQ=2)

                                                       gTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGGACGCATCGGTGCCCCCCCCGGCGTGGCCTGTTCGGGGTGCCCCGGCGGGGGGACGTCCGGCCCTCCGGGCAAT‑CCTCTGCTCGGGGAACAGGGGCTTCCCGGGGaaaac                                                                                                                                                                        >  2:1047796/1‑237 (MQ=14)

                                                       gTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGGTCAGCCGGGGTACGCATCGGGGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGGTGCGTCCGGACCTCCCGGCAAT‑CCGCTGCTCGGGGCAAAGGGCCTTTCCCGTGAAAc                                                                                                                                                                         >  1:786178/1‑238 (MQ=14)

                                                                  ccTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCCGCGGGGGGGCGTCCGGCCCTCCGGGCCAT‑CCGCCGGTCGGGGCAAAGGGCCTTCCCGGGGAAACCGGCATCcccc                                                                                                                                                              >  1:902813/1‑238 (MQ=14)

                                                                  ccTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGAAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCCGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCCGGGGGGGGCCGTCCCGCCAACCCGCCAAT‑CCCCTCCTCGGGGCCAAGGGCCTTCCCGGTGAAACCGAcctgcccc                                                                                                                                                              >  2:258906/1‑231 (MQ=14)

                                                                      tCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGGTCAGCCCGGGGACGCATCCGTTGCCCCACCGGCGTGGCCTGTTCCGCTGGCCCCGGCGGGGGGGCGTCCGGCCCTACCGCCCAT‑CCGCGGCCCGGGGCACAGGCCCGTCCCGGTGGAAACCACATCCCCCTgcc                                                                                                                                                          >  2:1071136/1‑238 (MQ=14)

                                                                      tCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGCAGCATTTTCCAGGGTTCAGCCGGGGTACGCACCGGGGCCCCCACCGGCGCGGCCTGTTCGGGGGGCCCCGGCGGGGGGGCGTCCGGCCCTCCCGGCCAT‑CCCCCGCCCCGGGCCAAGGTCCTTCCCGGGGGACACGCCAccgccccgcc                                                                                                                                                          >  2:651648/1‑238 (MQ=14)

                                                                                   aaCCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGt                                                                                                                                            <  2:74878/239‑1 (MQ=255)

                                                                                     cccGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGGGGCGGCCGGCCATCCGGTCAAT‑CCGCCGCTCGGTGCAAAGGGCCTTCCCGGTGGAACCGACATCGCCCCGCCCCGGCCCCTGCCCtt                                                                                                                                           >  1:1130031/1‑238 (MQ=14)

                                                                                          tAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATtct                                                                                                                                     <  1:461157/239‑1 (MQ=255)

                                                                                               cAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTTTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGGTCGGTGGGCCCCCGCGGGGGGGCGTCCGGCCCACCCGGCCAT‑CCGCCGCCCGGGGCCAAGGGCCCTGCCCGGGAAACCCACATCCCCCCTCCCCGCCCCCTGCCCTTCCCTCTCTcc                                                                                                                                 >  2:774653/1‑238 (MQ=14)

                                                                                                       aGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGGTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGGGTGCCCCGGCGGGGGGGCGTCCGGCCATCCCGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCCTTTTCTCCCCCACCTAc                                                                                                                        >  1:905534/1‑239 (MQ=14)

                                                                                                                                    cAGGGCGACATGGCGCAGGATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCgcgc                                                                                           <  1:820634/238‑1 (MQ=255)

                                                                                                                                          gACATGACGCAGTATGGCGGGAGCATTGTCCAGGGGTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTgg                                                                                     <  2:703702/238‑1 (MQ=255)

                                                                                                                                            cATGACGCAGTATGGCGGTAGCATTGTCCCGGGTTCAGCCGGGGGACGCATCGGTGCCCCCCCCGGCGTGGCCCGTTCGGGGGGCCCCGGCGGGGTGGCGTCCGGGCATCCCGGCAATCCCCCGGCCGGGGGCAAAGGCCTTCCCCGGGGAACCCAACA‑CCCCCCGCCCCGCCCCCTGCCCTTTCTTTTCTCCCCCCCCCCACGCAGTTTACCGGCCAAAAACGCCC‑CTCCCGTggggg                                                                                  >  2:1206995/1‑238 (MQ=255)

                                                                                                                                            cATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGGGGCCCGTTCGGTGTGCCCCGGCGGGGGGGCGGCCGGCCCTCCCGGCAAT‑CCCCCGCTCGGGGCAAAGGGCCCTCCCCGGGGAACCCACATCCCCCCGCCCGGCCCCCTGCCGCTCCTTCTCTCCCCCCCCCACAGCAG‑TTACCGGGCCAAAACGCCCGCGCCCggggggg                                                                                  >  2:900048/1‑238 (MQ=255)

                                                                                                                                                 cGCAGTACGGCGGTGGCATTGTCCAGGGTTCAGCCGGGGTCCGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGCGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAACCCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCt                                                                              <  2:517226/238‑1 (MQ=255)

                                                                                                                                                                       ccAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTg                                                       <  1:124016/239‑1 (MQ=37)

                                                                                                                                                                              tCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat                                                <  1:900048/239‑1 (MQ=37)

                                                                                                                                                                              tCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat                                                <  1:878114/239‑1 (MQ=37)

                                                                                                                                                                              tCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat                                                <  1:774653/239‑1 (MQ=37)

                                                                                                                                                                              tCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat                                                <  1:651648/239‑1 (MQ=37)

                                                                                                                                                                              tCAGCCGGGGTACGCATCGGGGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat                                                <  2:1176948/239‑1 (MQ=37)

                                                                                                                                                                                aGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCCG‑TTACCGGCCCAAAACCCCCGCGCCGGGGGGGGGCCTCGGCCCCCGCTGGAAAATGCCCGCgggtctc                                               >  2:54128/1‑233 (MQ=11)

                                                                                                                                                                                aGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGGCCTTCCCGGTGCAAACGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCCG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGCAAATGCCTGCCGataac                                               >  2:305124/1‑236 (MQ=255)

                                                                                                                                                                                aGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCCGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACCACTCCCTGCCCGGCCCGCTCCCGTTCATTCTCTCCCGCCCCTCCAACCG‑TTCACCGACAAAAACCCCCCCCCCGGTCGGGAGCCTCCGCCCCGGCGGGAAAATGCCTGCGGATATc                                               >  2:446632/1‑238 (MQ=11)

                                                                                                                                                                                       gTACGCATCGGTGCCCCCACCGGCGTGCCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCAGCCATCCGGACAAA‑CCGCTGCTCGGCGCAAAGGGACTTCCCGGCGAAACCGACAACGCCCTGCCCGGCCCGCCACCCTTCCTTCGCCCCCGCCCCTACATCAG‑TTCCCGCGCAAAAACGCCCCCGCCGGCGGGGCGACTGGGCCCCGGGTGGGCAATGCATGCGGactgccccttgt                                        >  2:961750/1‑227 (MQ=255)

                                                                                                                                                                                           gCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg                                   <  1:1047796/239‑1 (MQ=35)

                                                                                                                                                                                           gCAGCGGGGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg                                   <  1:661519/239‑1 (MQ=21)

                                                                                                                                                                                            acagggTGCCCCCACGGGCGTGGCCTGTTCGGGGTGCCCAGGCTGGGTGACGTCCGGCCAGCCGGTCAAC‑CCGCTGGTCGGTGCAAAGGTCCTTCCCGGTGAGACCGACATCGCCCTGCCAAGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGGGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg                                   <  2:832383/234‑1 (MQ=11)

                                                                                                                                                                                             tgcGGGGCCCCCACCGGCGGGGCCTGTTCGGGGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTgcg                                 <  2:516107/237‑1 (MQ=21)

                                                                                                                                                                                                  tGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGGGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGGGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGGGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGCGCTCCGCTTCCAGCTGCGCGGTa                            >  1:616177/1‑239 (MQ=11)

                                                                                                                                                                                                   gCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATaa                           <  1:788305/239‑1 (MQ=33)

                                                                                                                                                                                                    ccccccccGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATaa                           <  1:305124/238‑1 (MQ=18)

                                                                                                                                                                                                    cccccACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATaa                           <  2:275946/238‑1 (MQ=33)

                                                                                                                                                                                                     ccccACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAaca                         <  1:414813/239‑1 (MQ=33)

                                                                                                                                                                                                       ccACCGGCGGGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAacaca                       <  2:199540/239‑1 (MQ=18)

                                                                                                                                                                                                       caaCCGGCGTGGCCTGTTCGGGGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAacaca                       <  1:446632/237‑1 (MQ=18)

                                                                                                                                                                                                               gTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACt               <  1:1071136/239‑1 (MQ=33)

                                                                                                                                                                                                                tGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACt               <  1:926453/238‑1 (MQ=33)

                                                                                                                                                                                                                            tgtgCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAAcggc  >  1:1059852/1‑239 (MQ=33)

TTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAAT‑CCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG‑TTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGC  >  NC_000913/729475‑729933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A