Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F9 I0 R1 75 124.0 2465218 96.8% 2386331 230.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 989,943 T→G 21.5% S138S (TCA→TCC) ‡ pncB ← nicotinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913989,9430TG21.5% 127.5 / 8.0 85S138S (TCA→TCC) ‡pncBnicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (21/41);  new base G (15/7);  total (36/49)
Fisher's exact test for biased strand distribution p-value = 6.51e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCG  >  NC_000913/989707‑990178
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTg                                                                                                                                                                                                                                             <  2:348975/238‑1 (MQ=255)
gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTg                                                                                                                                                                                                                                             <  2:666187/238‑1 (MQ=255)
  tCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGag                                                                                                                                                                                                                                           <  2:116440/238‑1 (MQ=255)
       tCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGagcgatag                                                                                                                                                                                                                                     <  1:900339/239‑1 (MQ=255)
         ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGagcgatagcg                                                                                                                                                                                                                                   <  1:251466/239‑1 (MQ=255)
           cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGGTAAAGCCGAGAAAGCGGATAA‑ATTGC‑TTTCCA‑GCGGGTCGA‑GGGGTTGCGCAAAGTCGGCCTGCGGGGggcgagagcggt                                                                                                                                                                                                                                 >  1:890454/1‑237 (MQ=255)
            gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                <  2:444842/239‑1 (MQ=255)
            gTGAGGGAAAGCCGACGCGCCAGATCGTAGTGGCTGGTGCCCACAAACCGGGATTCCTGTTGCAGACGCTTAACGATGGCTTCTTGTACCTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                <  2:420003/239‑1 (MQ=255)
             tGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                <  2:789900/238‑1 (MQ=255)
                     aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATT‑CTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAAAGCCCGGAAGGAGAATAATTTTGT‑TTTCCA‑‑CGTGGCGG‑GGGGTTGTGCAAAGTCGGCCTGGGGGGAGCGTAAGCGGTGGACCC‑tttt                                                                                                                                                                                                                      >  1:1118632/1‑237 (MQ=255)
                          aCGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGCGAAGTCGAACAA‑TTTGC‑TTTCCA‑GCGGGTGGA‑GGGCGTGCGCAACGTCCGCCTGCGGGGGGGGCTAGCCGGGTGCCA‑TTTCCCttg                                                                                                                                                                                                                 >  2:1025507/1‑237 (MQ=255)
                           cgcgCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGAGGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGa                                                                                                                                                                                                                 <  1:91303/238‑1 (MQ=255)
                             cgcCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGGGAAGTCGGCTCA‑TTTTC‑TTTTCA‑GCGTGTCGG‑GGGGTTGCGGAAACGCGGGCTGCCGGGGGCGGCAGCGGTGTACCC‑TTTCTCTGATa                                                                                                                                                                                                               >  2:1126734/1‑238 (MQ=255)
                                  acgcGTAGTTGCGGGGCCCCACAAACCAGGATACCGTTGGCAGCCGTTTAACGATGGTTTTTTGTACTTCGCGAGAAAAACGGCGCC‑GGGGGCCAAAACCCATCAGATGGAAGCGCGACATACC‑AAGCCCGGCGGTTACCGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGGCGA‑GGGCTTGCGCAACTCCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGcc                                                                                                                                                                                                         <  2:1036234/236‑1 (MQ=255)
                                        aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAACCGGCGAC‑GGGTGCCAAAACCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCaa                                                                                                                                                                                                    <  1:952787/238‑1 (MQ=255)
                                        aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGGCGGCTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGGTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCA‑TTTCACTGATAAACGCCCGCaaa                                                                                                                                                                                                   >  1:1005922/1‑239 (MQ=255)
                                               ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGGTAGCGATGTACCC‑TTTCACTGATAACCGCCCGCAAAGGAACt                                                                                                                                                                                             >  1:477396/1‑238 (MQ=255)
                                               ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAAAGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTAACA‑TTTCACTGATAAACCCCCGCCAAGGaaat                                                                                                                                                                                             >  2:1083384/1‑236 (MQ=255)
                                                                    cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCC‑GCGTGTCGG‑GGGCGTGCGCAACGGCGGCCTGCGGTGGGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCCGCAAAGGAACATCCCAGAGGGTGAATTCACGc                                                                                                                                                                        >  1:1147389/1‑238 (MQ=255)
                                                                      gTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGGTAAAGCCGCGAAGTCGACTAA‑ATTGC‑TTTTCA‑GCGGGGCGG‑GGGCCTGCGCAAAGTCGGCCTTCGGTGTGCGGTAGCGGAGGACCA‑GTTTAATGATAAACCCCCGGCAAGGAACTTTCCCGGGGATGGATTTACGccc                                                                                                                                                                      >  2:563338/1‑237 (MQ=255)
                                                                        tgaaGACGGTTACGGAGGGTTTTTTGTACTGCGCGAAAAAACGGGCGAG‑GGGTGCAAAAATCCATCAGAGGGAAGCGCGACATACC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTCCCA‑GCGTGTGGA‑GGGCTTGCGCAACGTCGGCCTGGGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACg                                                                                                                                                                    <  2:696236/235‑1 (MQ=255)
                                                                         gCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCGG‑GGGGTTGCGCAACGACCGCCTGCCGGGGGCGGTAGCGCTGGAACA‑TTTCACTCATAACCGCCCGCAAAGGCACTTCCCCGCGGGTGACCTctcccccccg                                                                                                                                                                   >  2:373855/1‑229 (MQ=255)
                                                                            gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCCGGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGGGTCGG‑GGGGTTGCGCCAAGGCGGCCCGCGGGGAGCGCAAGCGATGTAACC‑TTTCTCTGATAAACCCCAGCAAAGGGAATTCCCCGGGGATGACTTCACGCCCCGGGcc                                                                                                                                                                >  2:383357/1‑238 (MQ=255)
                                                                            gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCCAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGCGTCGG‑GGGCTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCACGGGcc                                                                                                                                                                >  1:590856/1‑238 (MQ=255)
                                                                                             tCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAAAGCCGCGAAATCGCCTAA‑TTTTC‑TTTCCA‑GCGTGTCGG‑GGGCTTGCGCCACCGCGGCCTGCGGGGAGCGGTAGCGGTGTAACA‑TTTCACTGGTAACCCCCCCCCAAAGAACTTTCCCGAGGGTGAATTTTAGCCCCGGGCCCCTTTAAAGAatttaca                                                                                                                                               >  2:378376/1‑233 (MQ=255)
                                                                                               tttgaCTGCGCGAAAAAAGCGGCTAC‑GGGTCCCAATATCCAACAGAGGGAAGCGCGACAAATA‑GAGACCGGCGGTTAACGCCGAGAGGTCGACTAA‑TTTGC‑TTCCCA‑GCGGGTCGA‑GGGTTTGCGCAATTTCGGCCTGCGGTGAGCGATGAAGTTGTACAA‑TTTCCCTGAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCt                                                                                                                                            <  2:800021/235‑1 (MQ=255)
                                                                                                     ttCGCGAGAAAAACGGCGAG‑GGGTGCCAAAATCCATCAGAGGGAAGTG‑GAAATACCAAAGACCCGGGGTTAGCGCAGGAAGGTCCA‑TTA‑TTTTT‑TTTCCACGGTTCTCGA‑GGGCTTGCGAAACTTCGGCTTGGGGGGGGGGATGGGAATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                      <  2:418862/239‑1 (MQ=255)
                                                                                                     tgcgcgAGAAAACCGGGGGC‑GGGGGAAAAAACCAAACGAATGGAAGCGGGACATATC‑AGGCCGGGCGGTTACCGCCGAGAAGCCGACTAA‑TTTGC‑TTTCCA‑GCGTGTGGA‑GGGTTTGCGCAACGTCGGCCGGGGGTGAGCGAGAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                      <  2:259729/237‑1 (MQ=255)
                                                                                                       ggcgAG‑AAAACGGCGGCGGGGGGCCAAAAGCCATCAGACGAAAGCGCGACATTTC‑AAGCCCGGCGGTAAACGCCGAGAAGCCGCTTAT‑TTTGC‑TTCCAA‑GCGTGTGGA‑GGCCTTGCGCAACGTCGGCGTGCGGTGAGCGATAGGGATGTACCT‑TTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTa                                                                                                                                    <  2:606028/238‑1 (MQ=255)
                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGCGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTACCA‑TTTCACTGATAACCCCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACGCCACGGGGCGCCTAAAAGGAAATCCAACTTGCCAttt                                                                                                                                    >  1:1103764/1‑238 (MQ=255)
                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCCAGAAGTCGACTAA‑TTTTC‑TTTTCA‑GCCGGTCGA‑GGGCTTGCGCAACCACGGCCTGCGGGGAGCGATAGCGGTGTACCA‑TTTTACTTATAAACCCCCGCAAAAGAAATTCCCACAGGAAGACTTCACCCCACCGGCCGACTAAAAGAATATCCAACCTGTCATTa                                                                                                                                    >  2:737493/1‑239 (MQ=255)
                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAAAGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCCA‑GGGCTTGGGAAACATCGGCCTGCGGTGAGAGCTAAGGCTGTTCCA‑TTTCACTTAATAACGCCACACAAGAAACATCCCCGCGGGTGACCTCAACCCCCGCGGCCCTTAAACGGAAATCCaacctcacctta                                                                                                                                    >  2:742550/1‑228 (MQ=255)
                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGGTAACACCGAAAAGTAGACCAA‑TTTGC‑TTTTCA‑ACGTGGTGA‑AGGCTTTCGCAAAGTCGGGCTGCGGTTCGGGGTAGCGCGGAACCA‑GCTCATCGATTACCGCCACCAAACGAAGTTCCCCGAGGATGACGTCACTCCACGGCACCCTTAAAAGAATATCCAGCTTGGCAtgt                                                                                                                                    >  2:699412/1‑237 (MQ=255)
                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGCTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCCTGTCGA‑GGGGTTTCGCAAAGTCGGCCTGCGGGGGGCGCTAGCGATGTAACA‑TTTCACTGATAACCCCCAGCAAAGGAACTTCCCAGAGGATGACTTTACCCCCCGGGCCGCCTAAAAGAATATCCAGCTTGGCATTa                                                                                                                                    >  2:125872/1‑239 (MQ=255)
                                                                                                         cgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCCGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGTTAACCGCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACCCCACGGGCCGCCTAAAAGAAAAATCAGCTTTCCATTATc                                                                                                                                  >  1:105888/1‑239 (MQ=255)
                                                                                                             aaaaaCGGCGGG‑GGGTCGCAAACCCCACAAGATGGAACGGCGACATACA‑AAAGCGGGCGGTTAACGCAGAGAAGTCG‑CTTA‑TTTGCTTTTCCA‑GCGTGTGGA‑GGATTTGGCCAAGGTCGGCCTGGGGTGGGCGAAAGGGATGTCCCA‑TTTCATGGAGAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTg                                                                                                                              <  2:136731/239‑1 (MQ=255)
                                                                                                                                           agagGGAAGGGGGACATAAC‑AAGACGGGGGGTAACCGCGGAGAAGCCGACTAT‑TTTGC‑TTCCAG‑GGGGGGCGG‑GGGCTGGCGAAACGTCGGCCGGCGGGGGGCGATGGCGATGTCCCA‑TTCCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAg                                                                                                   <  2:590856/237‑1 (MQ=255)
                                                                                                                                               ggAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGGCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCGTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAAACACCCGCAAAAGAACTTCCCAAAGGGAGACTTCACGCCCCCGGGCGCCTAAAAGAAAATCCACCTCGCCATTATCGTTTGTCACGGTGACTTGTTTCGGGTTTAAGCGGaa                                                                                              >  2:79422/1‑238 (MQ=255)
                                                                                                                                                 aaGCGCGACATATC‑AAGACCGGCGGTTAAAGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAAAGTCGGCCCGCGGGGGGCGAAAGCGATGTACCA‑TTTCACTGATAACCGCCAACAAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCTTCAACGAAAATCCCACTTTCCATTTTCGTTTGACACGGTGACTTGGTCCGGGGTAAAGCGGaaacc                                                                                           >  2:48225/1‑236 (MQ=255)
                                                                                                                                                      cgACATATC‑AAGACCGGCGGTTAAAGCCGAGAAGTCGACTAA‑TTTTC‑TTTTCC‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCA‑TTTTACTGATAACCGCCCGCAAAAGAAATTCCCAGAGGGTGACTTCCCGCCACGGGCCCCTTAAAAGAATATCCAGCTTTCCATTATCGTTGGACACGGTGACTTGTTCCGGGGTTAAGCGGAACTCGCGTa                                                                                      >  1:822188/1‑239 (MQ=255)
                                                                                                                                                       gacaagtC‑AAGACCGGGGGATAACGCCGAGAAGTCGACGAT‑TTTGC‑TTTCCG‑GGGGGTCGA‑GGGCTTGCGCAACGCCGGCCTGCGGTGGGTGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:671591/233‑1 (MQ=255)
                                                                                                                                                       gACATTAC‑AAGCCGGGGGGTTATCGCCGAGAGGTCGGCTAA‑TTGTC‑TTGCCG‑TCTGTTCGG‑GGGCTGGCAAAACGCCGCCGGGGGGGGGGCGATAGCGTGGTACCA‑TTCCTCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:1118632/239‑1 (MQ=255)
                                                                                                                                                       gACATATC‑AGGCCCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTGGCGCAACGTCGGCCTGCGGTGGGCGATAGCGATGTCCCT‑TTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  1:48225/239‑1 (MQ=255)
                                                                                                                                                        atatatA‑AAGACCGGCGCTCACGGGCGAGAAGTCGCCTAA‑TTTGC‑TCTCCA‑GCGTTTCGA‑GGGCTCGCGCAAGGTGGGCCGGCGGTGAGCCAAGGCGATGTACTA‑TTTCACTGAGCGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTGCGCGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:608915/236‑1 (MQ=255)
                                                                                                                                                         acagtaaaggcccGGGGTTTCACGCCGAGAAGCCACTTAT‑TTTTC‑TTCCCT‑GCGTGGCGT‑GGGTTTGCGAAACGGCGGCCTGGGGTGAGTGAATGCGTGTTACCA‑TTTAACTGAGACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:128173/227‑1 (MQ=255)
                                                                                                                                                         aaatacgaaGACGGGCGGTTAGCGCCGAGAAGTCGGCTAT‑TTTGC‑TTCCCG‑GCGTGGCGA‑GGTCTTGCGAAACGCCGGCCTGGGGTGAGCGATAGCGAGTTACCA‑TTTCACTAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:1147389/231‑1 (MQ=255)
                                                                                                                                                          aatagcagacccGGAGTTTAACGCCGAAAAGTCGTCTAT‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTGGCGAAGCGCCGGCCGGGGGTGAGGGATAGGGATGTACCA‑TTCCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAc                                                                                    <  2:105888/228‑1 (MQ=255)
                                                                                                                                                             tC‑AAGACCGGCGTTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTCCCA‑TTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGtt                                                                               <  1:506583/239‑1 (MQ=255)
                                                                                                                                                              c‑AAGGCCGGCGGTAAACGCCGAGAAGCCGTCTAA‑TTTGT‑TTCCCA‑GGGTGTCGA‑GGGCTTGCGCAACCTCGGCGGGGGGGGGGCGAAAGCGATGGCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTa                                                                              <  2:101964/239‑1 (MQ=255)
                                                                                                                                                                     cggcggTTAACGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GAGTGGCGA‑GGGCTTGCGCAACGTCGGGCCGCGGGGAGCGCTAGCGATGGACCA‑TTTCACTGATAACCGCCCGCAAAGGAAATTCCCAGAGGATGACTTCACGCCACCGGCCGCTTAAACGAATATCCAGCTTGCCCTTATCGTTGGCCACGGGGACCTGTTCCGGGGTAAAACGGGACTCGCGGAAACAGTTAAGATaa                                                                        >  2:770658/1‑238 (MQ=255)
                                                                                                                                                                      ggcggTTCACGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGG‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCTCTGATAAACCCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCCCGGGGCGCTTAAACGAATATCCAGCTTGCCATTATTGTTGGACCCGGTGACTTTTTCCGCGTTAAAGCGGCCCACGCTGAACCAGCTAAAATat                                                                        >  2:865326/1‑237 (MQ=255)
                                                                                                                                                                       ggggTTACCGCCGAGAAGCCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                       <  1:1083384/236‑1 (MQ=255)
                                                                                                                                                                       gcggTTAACCCCGAGAAATCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGGATCC‑TTTCACTGATAACCGCCAGCCAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCCTAAACGAAAATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc                                                                      >  2:162345/1‑239 (MQ=255)
                                                                                                                                                                       gcggTGACCGGCGAGAAGTGGATAAT‑TTTGC‑TTTCCG‑GGGTGTCGA‑GGGTTGGCGAACCGCCGGCCTGGGGAGGGCGATAGCGATGACCCA‑TTTCACGGATAGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                       <  2:508199/238‑1 (MQ=255)
                                                                                                                                                                            tagcgacGAGAAGTCTACTAA‑TTTGC‑TTTCCA‑GCGGTTCGA‑GGTCTTACGCACCGTCGGCCGGCGGTGAGGGATAGCGATGTACCA‑TTCCACTATTAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  1:956524/233‑1 (MQ=255)
                                                                                                                                                                            taaggcgaagagGTCGACTAA‑GTTGC‑TTTCCT‑GCGTGTCGG‑GGGCTGGCGCAGCGGCTGCCGGGGGTGAGCAATAGGGATG‑ACCATTTTCACTAAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  2:373052/231‑1 (MQ=255)
                                                                                                                                                                            tAACGCCGAGAAGTCGTCTAT‑TTTGC‑TTTCCG‑GCGTGTCGG‑GGGTTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCT‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  1:383357/239‑1 (MQ=255)
                                                                                                                                                                            tAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  1:770658/239‑1 (MQ=255)
                                                                                                                                                                            tAACGCCAAGAAGCCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGCCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  1:860154/239‑1 (MQ=255)
                                                                                                                                                                                 ccGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaag                                                            >  2:261482/1‑239 (MQ=255)
                                                                                                                                                                                         ccGACTAT‑TTTGC‑TTCCAA‑GCGTGTGGA‑GGGCTGCCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAata                                                                                                                                                   <  2:1051242/143‑1 (MQ=255)
                                                                                                                                                                                          cGACTAA‑TTTGC‑TTTCCA‑‑CGGGTCGACGTGCGGGGCAAACGTCGGCCGGCGGTGCGGCATAGCGTTGGCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggca                                                     <  2:553477/237‑1 (MQ=255)
                                                                                                                                                                                                a‑TTTGC‑TTCCCG‑GGGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGGGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                             <  1:756915/239‑1 (MQ=255)
                                                                                                                                                                                                a‑TTTGC‑TTCCCA‑GCGTGTCGG‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTCCCA‑TTTCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                             <  1:31666/239‑1 (MQ=255)
                                                                                                                                                                                                    tGC‑TTTCCG‑GCGTGTGGG‑GGGCTTGCGCAACGTCGGCCTGGGGTGGCAGATAGCGATGTCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc                                          <  1:368407/239‑1 (MQ=255)
                                                                                                                                                                                                    tGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGc                                                                                                                                         >  1:1051242/1‑144 (MQ=255)
                                                                                                                                                                                                    tGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGACCTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAg                                           >  2:458893/1‑238 (MQ=255)
                                                                                                                                                                                                      c‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                        >  2:168276/1‑95 (MQ=255)
                                                                                                                                                                                                      c‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCGGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                        <  1:168276/95‑1 (MQ=255)
                                                                                                                                                                                                        ttcccA‑GCGTGTGGG‑GGGCTTGCGCAACGCCGGCCGGCGGTGAGCGATAGCGATGTACCA‑TTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAc                                       <  1:1025507/236‑1 (MQ=255)
                                                                                                                                                                                                         tttcaggCGTGTCGA‑AGGCTGGCGCACGGTCGGCCTGCGGGGAGCGATGGGGATGTCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAc                                       <  1:499859/233‑1 (MQ=255)
                                                                                                                                                                                                          tCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTg                                     >  1:635268/1‑239 (MQ=255)
                                                                                                                                                                                                          tCCA‑GCGTGTCGA‑GGGCTTGCCCAACGTGGGCTTGCGGTGGGCGATAGCGATGTCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTg                                     <  1:125872/239‑1 (MQ=255)
                                                                                                                                                                                                                               gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAc                                                                                    >  1:736279/1‑173 (MQ=255)
                                                                                                                                                                                                                               gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                   >  1:232185/1‑238 (MQ=255)
                                                                                                                                                                                                                               gcgaAACGTCGGCCGGGGGGGAGCGAGAGCGATGTCCCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAgg                  <  2:822188/239‑1 (MQ=255)
                                                                                                                                                                                                                                cgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgc                 >  1:1188491/1‑239 (MQ=255)
                                                                                                                                                                                                                                 gcAACGTGGGCCTGCGGTGAGCAATAGCAATGTCCCC‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcg                <  1:733799/239‑1 (MQ=255)
                                                                                                                                                                                                                                   atgtccGGCCTGCGGTGAGCAAAAGCGTGTTCCCA‑TTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc               <  1:1225322/233‑1 (MQ=255)
                                                                                                                                                                                                                                   aaCGTCGCCCTGCGGTGAGCGATAGCGATGTCCCA‑TTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc               <  1:584196/238‑1 (MQ=255)
                                                                                                                                                                                                                                            cTGCGGTGGGGGATAGCGATGTCCCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa     <  2:1221535/239‑1 (MQ=255)
                                                                                                                                                                                                                                            cTGCGGTGAGCGATGGCGATGTACCA‑TTTCACGAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa     <  1:373855/239‑1 (MQ=255)
                                                                                                                                                                                                                                               cGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc   >  2:25405/1‑238 (MQ=255)
                                                                                                                                                                                                                                               cGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc   >  2:934389/1‑238 (MQ=255)
                                                                                                                                                                                                                                                 gTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCg  >  1:825185/1‑237 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
GTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCG  >  NC_000913/989707‑990178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A