Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F18 I0 R1	150	105.7	2655328	79.3%	2105675	226.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,383,718	C→A	22.4%	E82* (GAA→TAA)	ycjW ←	LacI family putative transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,383,718	0	C	A	22.4%	117.8 / 6.2	65	E82* (GAA→TAA)	ycjW	LacI family putative transcriptional repressor
Reads supporting (aligned to +/- strand): ref base C (41/7); new base A (2/13); total (44/21)
Fisher's exact test for biased strand distribution p-value = 5.87e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATAAA  >  NC_000913/1383488‑1383948

ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGTCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCTGGATTGTGCGCCGTTTGTAATATCACGTCACAACTTTCTTCTTCCGCTTTGGCGGTGTTTGCATCCACAACTTccatgaaaa                                                                                                                                                                                                                                     >  2:1071400/1‑231 (MQ=255)

ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGCCGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGAAAAAATTCaaaaaaaa                                                                                                                                                                                                                                     >  2:199012/1‑239 (MQ=255)

ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTACTATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGTTGGCATGTAAAACTTACGAGaaaa                                                                                                                                                                                                                                     >  1:1007184/1‑239 (MQ=255)

ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGaaaa                                                                                                                                                                                                                                     >  1:676171/1‑239 (MQ=255)

ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATAACGTCGAAACCTTCTTCTTCACCTTTGGCGGGGATGGCATGCAAAACTTTAGAGaaaa                                                                                                                                                                                                                                     >  2:851970/1‑239 (MQ=255)

    tatTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGTGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAAATTCTAGGAAAAC‑Cgg                                                                                                                                                                                                                                >  1:405727/1‑239 (MQ=255)

           cTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTCCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGAGTCTATGCAAAACTTCAACAACAAA‑CGGATAAcc                                                                                                                                                                                                                          >  2:902141/1‑238 (MQ=255)

                     gCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGttt                                                                                                                                                                                                              >  1:189979/1‑239 (MQ=255)

                                ccaccGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACgg                                                                                                                                                                                                   >  1:1219566/1‑239 (MQ=255)

                                   ccGGAATATCATATTTATCGGGTTGGGAAAAAAATGA‑TCACTCCGTGGGGCAACTGAGCAAAATAATGCCTTTAATCATTTTCTGCTTATTTTTGTTTTCGCTTTTTTGCAGTTCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTAAGAAAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTa                                                                                                                                                                                                <  1:1057574/239‑1 (MQ=255)

                                           tCATATTTATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCa                                                                                                                                                                                         >  1:1108545/1‑238 (MQ=255)

                                                 ttATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATAACCATAAt                                                                                                                                                                                  >  1:552028/1‑239 (MQ=255)

                                                   aTCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGATCATAATAATGCCTTTAATCATTTTCTGCTTAATTGTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTTCGACGTTTGTAATATCCCGTCGTAACCTTCTTCTTCAGCGTTGGAGGGGGTGGCATTCAAAACTTAAGGGCAAAA‑CAGATAACCCGCC‑GTAGTGTTGGCGGAACAAGAAGAAAtacccaaaaggg                                                                                                                                                                                >  2:1065707/1‑228 (MQ=255)

                                                     cGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCa                                                                                                                                                                             >  1:167846/1‑239 (MQ=255)

                                                     cGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTACTTTTGTCCGAACGGGAAAAAAAAACCAAAAT‑GGCa                                                                                                                                                                             >  2:93901/1‑239 (MQ=255)

                                                      gAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGCTTGTGCGACGTTTGTACTATCACGTCGAAACCTTCTTCTTCCGCTTTGGCGGCGATGCTATGGAAAACTTCCGAGACAAA‑CGGATTACCCACCGGTGTTTTTGGTC‑AACGGGGAGAAATCACCaaaaagagct                                                                                                                                                                             >  2:261425/1‑233 (MQ=255)

                                                         ttGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGTTTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGAtt                                                                                                                                                                                                                             <  1:342781/189‑1 (MQ=255)

                                                         ttGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCCAAACTTCAAAGAAAAA‑TGGATTACCCCCC‑GTTGTTTTGGGCCAAACGGTAGAAAACACCACaaaaccaaaca                                                                                                                                                                          >  2:544640/1‑230 (MQ=255)

                                                         ttGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGGGAAAAA‑CGGATTTCCCGCC‑GTAGTTTTGGTCGAAAGGGTAGAAAAAACCAAAAT‑ggaaaaaa                                                                                                                                                                         >  2:128759/1‑233 (MQ=255)

                                                         ttGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGAtt                                                                                                                                                                                                                             >  2:342781/1‑189 (MQ=255)

                                                           gggCAAAAAAGGCCTCA‑TCGGCGGGCAAACTGAAAATAATAATGCCTTAAATCTTTTTCTTTTTAATTTTGTTTTCGCTTTTTTGCAGTTCTTCTGCCGGATTGTGGGACGTTTGAAATACCACGGCGAAACTTTCTTCTCCAGTTTTGGGGGTGATGGCAGGAAAAACTCAAGAAAAAAA‑CGGATTCCCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCaaa                                                                                                                                                                        <  1:851970/238‑1 (MQ=255)

                                                                      gACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAAAggc                                                                                                                                                            >  1:1035964/1‑238 (MQ=255)

                                                                                       aaCTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTTCCCGCC‑TTAGTTTTGGTCGACAGGGTAGAAATCACCAAAAT‑GGCACAAAAAACCGAAAAGGGTAGGGCGC‑GGGCCAga                                                                                                                                           >  2:1003052/1‑238 (MQ=255)

                                                                                       aaCTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCa                                                                                                                                             >  1:115869/1‑237 (MQ=255)

                                                                                           gAGTATAATAATGCCTTTAATTATTTTCTTTTTAATTTTGTTTTCCCTTTTTTGCAGATCTTCTGCCGGATTGGGCGACGTTTGAAATATCACGTCAAACCTTTCTTCTCCGGTTTTGGGGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGt                                                                                                                                       <  1:635890/239‑1 (MQ=255)

                                                                                                   taatGCTTTTAATCTTTTTCTGTTTATTTTTGCTTTCCTATTTTTGCAGATCTTCTGCGGGATTGTGCGACGTTTGAAATATCAGTGCGAACCTTTCTTCTTCAGTTTTGGGGGTGAGGGCTGGCAAAACTTCAAAAAAAAA‑CGGTTTCCCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGa                                                                                                                               <  1:199012/239‑1 (MQ=255)

                                                                                                   taatCCCTTTAATCATTTTCTTTTTATTTTTGCTTTCGCTTTTTTGCAGTTCTTCTGCCGGATTGTGCGACTTTTTAAATTCCACGTCGAACCCTTCTTCTTCAGTTTTGGCGGTGATGGCATGAAAAACTTCAGAAAAAAC‑CGTATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGa                                                                                                                               <  1:128759/239‑1 (MQ=255)

                                                                                                      tGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGAt                                                                                                                              >  1:1005064/1‑237 (MQ=255)

                                                                                                        ccTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTATTATAACTTCGAAACCTTCTTCTTCAGCTTTGGCGGTGTTGGCATGCTAAACTTCAGCAGAAAT‑CGAATTACCCGCC‑GTAGCTTTGTTCGACTTGTTAGTAATCACCCTAAT‑AGCATCACAATCCGAAGAGGTCATCACGC‑GAGCCACCCTGCTTGGCTggatcgt                                                                                                                          >  1:91143/1‑233 (MQ=255)

                                                                                                        ccTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTCGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGAt                                                                                                                              >  1:150392/1‑235 (MQ=255)

                                                                                                        ccTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGCAGAGGGCAGCCCGC‑GGGCCCGCTTGTTTGGCTGAAACTg                                                                                                                          >  1:149865/1‑239 (MQ=255)

                                                                                                        ccTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATAACCATAAT‑TGCATCAAAACCCGAAGAGGTCA‑CCCGCGGGGCCAGCTTGTTTGGGTGGTaaag                                                                                                                          >  2:1291302/1‑236 (MQ=255)

                                                                                                        ccTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GGAGTTTTGGTCGAAGGGGGAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGACC‑GGGCCAGCCTGTTTGGccgcaactg                                                                                                                          >  1:1286818/1‑231 (MQ=255)

                                                                                                           ttAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTaa                                                                                                                       >  1:1261537/1‑239 (MQ=255)

                                                                                                           ttAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTaa                                                                                                                       >  1:489449/1‑239 (MQ=255)

                                                                                                           ttAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCAGAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCCC‑GGGCAAGCTTGTTTGGCTGATACTGTaa                                                                                                                       >  1:765702/1‑239 (MQ=255)

                                                                                                           ttAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGGTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGCCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTaa                                                                                                                       >  1:1064123/1‑239 (MQ=255)

                                                                                                           taaattgttttCTGTTTAATTTTTGTTTCGTATTTGTTCAGTTCTTCGGCGGGTTTGTGGGACTTTTGAAATATCCCGTCAAAACCTTCTTCTCCAGTTTTGGGGGTGATGGCATGCAAAACTCAAGAAAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTaa                                                                                                                       <  1:693657/232‑1 (MQ=255)

                                                                                                             aaTCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCTTCAAAACCCGAAAAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGGAAtt                                                                                                                     >  1:1070691/1‑239 (MQ=255)

                                                                                                                                   cTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGCAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAAAACttt                                                                                               >  1:1174247/1‑239 (MQ=255)

                                                                                                                                      tCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTTATTGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGATCGGGTAGAAATCACCATAAT‑GGCATCAAAAACCGAAGAGGTCAGCGCGC‑GGGCCAGCCTGTTTTGCTGATACTGTAATTCTTCAATGGGCCGGAAACCTTTTTc                                                                                            >  2:811084/1‑239 (MQ=255)

                                                                                                                                       cGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGTCTGATACTGTAATTCTTAAATGGCCCGGAAAACTTTTTc                                                                                            >  2:928133/1‑238 (MQ=255)

                                                                                                                                           ttgttGCAGATCTTGTGGCGGTGTGTGGCACGTTTGAAAATTCCCGTCAAACCCTTCTTCTGCAGTTTTGGCGTGGAGGGAATGCAAAACTTAAGAAAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTcgcgcg                                                                                       <  1:843781/236‑1 (MQ=255)

                                                                                                                                                  aGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGt                                                                                                                                                            >  1:328534/1‑164 (MQ=255)

                                                                                                                                                    aTCTTCTGCCGGATTGTGCGCCGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATTCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCCAAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGGAACTGTAATTCTTCAAAGGCCCGCAAACCTTTTTTCGGGGCTTCCcggg                                                                              >  2:849119/1‑239 (MQ=255)

                                                                                                                                                              gattggggcggcgTTTGAAAACTCACGCAGAACCCTTTTTCTGCGGTTTGGGGGGTGATGGCATGAAAAATTTAAGAAAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAg                                                                     <  1:544640/231‑1 (MQ=255)

                                                                                                                                                                 tggggCGACTTTTGGAATACCACGTCGAAACCTTCTTCTTCAGCTTGGGCGGTGAGGGCATGAAAAACTTAAGAAAAAAA‑CGGATTCCCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTg                                                                 <  1:93901/235‑1 (MQ=255)

                                                                                                                                                                    tgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGGTACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCtt                                                              >  1:651046/1‑239 (MQ=255)

                                                                                                                                                                           ttttTAATATCCCGCCAACACCTTTTTCTCCATTTTGGGCGTTGATGGAATGCAAACTTTAAAAAAAAAA‑CGAATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAg                                                        <  1:738582/238‑1 (MQ=255)

                                                                                                                                                                              aaaaTACCACGGAGAAACCTTCTTTGTCAGGTTGGGCGGTGAGGGCATGAAAATCTTAAAAAAAAAG‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑Acg                                                   <  1:1095974/237‑1 (MQ=255)

                                                                                                                                                                                     cACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATa                                            >  1:491543/1‑239 (MQ=255)

                                                                                                                                                                                       cGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGAATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATAACCATAAT‑GGCCTCAAAACCCGAAGAGGTCAGCGCGC‑GGGCAAGCTTGTTTGGCTTATCCTGTAATTCTTCAATGGCCCGGAACCCTTTTTCGCGCGCTTTCGGGGGGAAATTAGATTGGTTTTTCAGA‑Acgctgtaacgg                                          >  2:744682/1‑231 (MQ=255)

                                                                                                                                                                                            aaaCCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAAAACCTTTTCGCGCGCTTCCGGGGAGCTATTAGTTTGCTTATTCAGC‑ACCCGGGTTACgtcg                                       >  1:139133/1‑234 (MQ=255)

                                                                                                                                                                                                  tcttcttcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCCGGATACGGTGGAttttta                               >  1:196407/1‑237 (MQ=255)

                                                                                                                                                                                                   tttgttcAGCGTGGGGGGTGATGCAATACAAATCTTAAGAGAAAAC‑CGGATTCCCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGAt                              <  1:744682/235‑1 (MQ=255)

                                                                                                                                                                                                   tttcttcCGTTTGGGGGGTGAGGGGATGAAAAACTTCGAAAAAAAC‑CGGTTCCCCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGAt                              <  1:647738/238‑1 (MQ=255)

                                                                                                                                                                                                          aGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGATACg                           >  1:901573/1‑236 (MQ=255)

                                                                                                                                                                                                           gCTTTAGCGGTGATGGCATGCAAAACTTCAGAGAAAAC‑TCGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGAATCAAAAACCGAAGGAGTCAGGGCGC‑GGGCCATCTTGTTTGGCTGATACTGTAATTATTCAATTGCCTGATCCACATTTTCTGGGGCTTCCGGGGGGGTCTTGATTTGCTTTTTAGCCAATGCG‑GTTACGAGGGATTTTGATAGGCCTGc                      >  2:278121/1‑239 (MQ=255)

                                                                                                                                                                                                                     ggtgtcctgaaaaacCTTAAGAAAAAAA‑CTTATTCCCCGCC‑GTAGTGTTGGGCGAACGGGTAAAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa            <  2:651046/228‑1 (MQ=255)

                                                                                                                                                                                                                      gATCGCATGCAAACCTTCAGAGAAAAC‑AGAATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATGCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa            <  1:261425/238‑1 (MQ=255)

                                                                                                                                                                                                                       tgtgggttgaaaaATTGAGAAAAAAATGGGTTTCCCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa            <  2:552028/229‑1 (MQ=255)

                                                                                                                                                                                                                        gggCATACAAAGCTCAAGAGAAAAC‑CGGTGTTCCCGCC‑GGAGTTTGGGTCGAACGGGTAGAAACCCCCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAata         <  1:602586/238‑1 (MQ=255)

                                                                                                                                                                                                                         ggaaTGAAAACCTTAAGAAAAAAC‑GGGATTCCCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAata         <  1:339293/235‑1 (MQ=255)

                                                                                                                                                                                                                         ggaaTGAAAAACTTAAGAAAAAAA‑CGAATCCCCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAata         <  1:1291302/235‑1 (MQ=255)

                                                                                                                                                                                                                             tgataacgttCAAAGAAAAA‑CGGTTTCTCCGCC‑GTACTTTTGGTACAACGGTGAGAAATCACGAAAAT‑GGCTTCAAAACCCGAAAAGGTCAGCGCGC‑GGGACTGGTTGTTTGGCTGATGCTGTAATTATTCAATTGCCCGTAACACTTTTTCGTGAACTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGATACGGCTGCAACCCTGGAAATAGCAt     <  2:58352/230‑1 (MQ=255)

                                                                                                                                                                                                                               cAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCTCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCCGC‑ACGCGCGATCCGGGGGCTTTTGATACGCCTGCAACCCTGGCAaaatagaaaa  >  2:1007842/1‑233 (MQ=255)

GGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCA‑TCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCC‑GTAGTTTTGGTCGAACGGGTAGAAATCACCATAAT‑GGCATCAAAACCCGAAGAGGTCAGCGCGC‑GGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGC‑ACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATAAA  >  NC_000913/1383488‑1383948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A