Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F17 I0 R1	92	90.7	2214160	82.3%	1822253	222.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,879,936	T→G	22.3%	T62P (ACC→CCC)	gyrB ←	DNA gyrase, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,879,936	0	T	G	22.3%	127.8 / 6.9	68	T62P (ACC→CCC)	gyrB	DNA gyrase, subunit B
Reads supporting (aligned to +/- strand): ref base T (18/33); new base G (15/2); total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.77e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATTTTACCCTCGCGCTGGATAACCAGCTCCA‑GTTTTTGCGACAGGGCGTTTACT‑ACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACC  >  NC_000913/3879699‑3880165

tatatttCCCCGCGCGGTGGATACCCAG‑TCTAGGTTTGTGGGACAGGGC‑TTTGCTCACCGAACCACCACCGCCGTGCAGACCGCGGGATCTTTTAT‑GGGTGTTAGCGCCAAA‑TTTGCCGCCTGCGTGCAGAACGGTCATGATCACTCCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTg                                                                                                                                                                                                                                      <  2:320698/235‑1 (MQ=255)

           tCGCGCTGGATAACCAGCTCCA‑GTTTTTGCGACAGGGCGTTTACT‑ACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGGGCAGAACGGTCATGATCACTTCCGCCGCCGATCCGCCCCCTTCCGGGAGAATACCGGTCGGACTCCCGCGCCCGACATCC‑TGT‑ACAGAGACAGAGGTATAGAGGAGGATGGGGACGAAAAta                                                                                                                                                                                                                             >  2:309357/1‑236 (MQ=255)

           tCGCGCTGGATAACCAGCTCCA‑GTTTTTGCGACAGGGCGTTTACT‑ACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGGGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCTCCTTCCGGGTGAATACCGGTCGGAATCCCGCGCCCGCCATCC‑TGTAACAG‑GCAAGGGTTATCGGCGTGGAGGGGGAGGAAAAtttt                                                                                                                                                                                                                           >  2:698734/1‑238 (MQ=255)

                             tCCA‑GTTTTTGCGACAGGGCGTTTACT‑ACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGGGCGGGTAATTTTTTTCCACGGACCCCCGAg                                                                                                                                                                                                         >  1:229065/1‑239 (MQ=255)

                             tCCA‑GTTTTTGCGACAGGGCGTTTACT‑ACCGAAACACCAACGCCGGGCAGACCGCCGGACACTTTAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGCCAGAGTTATCGGCGTGCATGTGGACGAGAATTTTTTTACAGGGACCcgcgc                                                                                                                                                                                                          >  1:1107007/1‑237 (MQ=255)

                                       tGCGACAGGGCGTTTACT‑ACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGGGAAAGGGGACGGTAATTTCTTTACAGTGACCCGCGAGCGCTtcgtc                                                                                                                                                                                                >  2:125018/1‑239 (MQ=255)

                                          gtcggggCGTTTCTG‑ACCCAACCCCCAACGCGTGGCAGACCGCC‑GACAATTTTTAAGGAGTTATCACAAAA‑TTTACCCACTGGGTGAAGAAGGGTCAGGATCATTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGa                                                                                                                                                                                              <  2:870482/234‑1 (MQ=255)

                                          gACAGGGCATTTACT‑ACCGAAACCCAAACGCCGTGCAGACCGCCGGACACTTTAT‑AGGGTTTATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGa                                                                                                                                                                                              <  1:703526/238‑1 (MQ=255)

                                                                       cGCCGTGCAGCCCGCGGGACACTTTAT‑AGGAGTTACCGTCAAA‑TTTCCCGCCTGCGTGCAGAACGGTAATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCAtg                                                                                                                                                                 <  1:962813/239‑1 (MQ=255)

                                                                            tGCAGACCGCCGGACACTTTAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGCCAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCCTCGGCGTTAGAGGTATCTACAACCCCGAATaaccagtgggg                                                                                                                                                            >  2:213861/1‑229 (MQ=255)

                                                                            tGCAGACCGCCGGACACTTTAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGGGGCGATAATTTCTTTTCAGTGACCCGCCAGCGCTTCGTCGATAGAGGTATTAACCACCCCCAAAACCaattgggg                                                                                                                                                            >  2:168495/1‑232 (MQ=255)

                                                                            tGCAGACCGCCGGACACTTTAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGGGACGGTAATTTCTTTaaa                                                                                                                                                                                                                     >  1:181600/1‑180 (MQ=255)

                                                                                         actcTTTAA‑AGGGTTTACCGTAAAG‑TTGCCCGCCGGCGTGCAGAACGGTCATGATCACTCCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGcc                                                                                                                                                <  1:125018/235‑1 (MQ=255)

                                                                                             ttttaagggAGTTTCCGTAAAT‑TTTCCCGCCTGCGTGCAGAACGGTCATGATCCCTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCa                                                                                                                                            <  1:413180/232‑1 (MQ=255)

                                                                                             tttAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAAAGGTGACGATAATTTCTTTTCAGTGACCCGCGAGCCCTTCGTCGGTGGCGGTTTATACCACCCCCAATACCACGTGGGGGAGACCGGTCCCGGCa                                                                                                                                            >  2:761159/1‑238 (MQ=255)

                                                                                                       gTTTTCGTCATT‑TTTCCCGCTGGCGTGCGGAGCGGTCAGTGTCCCTCCCGCCGCCTGTACGCCCTGTTACGGGTGAACGCCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCg                                                                                                                                   <  2:691464/238‑1 (MQ=255)

                                                                                                        ttATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCTCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCCTCGGAGCTAGCGTTAACTAACACCTCGAAAAACATGTGGTGCCGACCGGGGGCGGCAACCGTGACGca                                                                                                                                 >  2:793951/1‑238 (MQ=255)

                                                                                                        ttATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGGGCCGGTAATTTCTTTACAGTGCCCCCCGGGCGCTTCGCGGTTAGCGGTATTTTACACCCCGAATACCCTTTGGTGAACACCGGAGCCGTCACCCggtttcc                                                                                                                                  >  2:64312/1‑232 (MQ=255)

                                                                                                         taccGTCAAA‑TTTCCCGGCTGTGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCg                                                                                                                                <  1:1012611/236‑1 (MQ=255)

                                                                                                          ttCGTCAAATTTTCCCGCGTGCTGGCAGAACGGTCAGGATCCCTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATGCCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCg                                                                                                                                <  2:46096/238‑1 (MQ=255)

                                                                                                          aTCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATTTGGTGCAGACCGGGGCCGTCATCCGGGTCGcccg                                                                                                                               >  1:92112/1‑237 (MQ=255)

                                                                                                          aTCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCGCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ATAGTGACAGAGTTATCGGCGTGCATGGTGACGATAATTTCTTTACa                                                                                                                                                                                                                     >  1:472115/1‑153 (MQ=255)

                                                                                                                         cGCCTGCGGGCAGAACGGGCAAGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCggg                                                                                                                 <  1:213861/239‑1 (MQ=255)

                                                                                                                                           tcctGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCggg                                                                                                                 <  1:391191/218‑1 (MQ=255)

                                                                                                                                                aTCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGGGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGCTAGCGTTATCTACCACCCCGAATACCAGTGGGTGCCGACCGGGGCCGCCCTCCGTGTCGCCGCAATACATAACCCGGCGCTTACGCACCGCACCCAGc                                                                                            >  2:339936/1‑237 (MQ=255)

                                                                                                                                                    ctccccccgccgATACCCCCTATTCCGGGTGAATACCTGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTTACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTc                                                                                      <  2:184758/233‑1 (MQ=255)

                                                                                                                                                     ttCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGGGGCGCTAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGAATGCGTTATCTTCCACCTCGAAAACCATGTGGGGCAGACCGGGGCCGTCACCCCTCTCCCCCGTTTACATACCCGGGCGCTTCCGCCCCCCAACCAAcccatccg                                                                                     >  2:568964/1‑234 (MQ=255)

                                                                                                                                                           cgccgATTCGCCCTCTTCCGGGGTAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACtt                                                                               <  2:92112/239‑1 (MQ=255)

                                                                                                                                                             ccgATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGAt                                                                                                                                                                                                                                 <  1:244374/91‑1 (MQ=255)

                                                                                                                                                             ccgATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGGGACGAt                                                                                                                                                                                                                                 >  2:244374/1‑91 (MQ=255)

                                                                                                                                                                 tACGCCCGCTGCCGGGTGAATACGGGTCGGAATGCCGCGCACGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTCACGATAATTTCTTTACAGTGGCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATGCCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATa                                                                          <  1:64696/238‑1 (MQ=255)

                                                                                                                                                                     cccTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTTCCGTCATCCGTGTCGCCGATATACATACCCGCGCGCTTTCGCACCCCATCCAGCCCTTTTCGGACTTTCATACTtga                                                                     >  1:479352/1‑239 (MQ=255)

                                                                                                                                                                      cctcctCCGGGGTAATCCCGGTCGGAATGCCGCGACCGTCATCC‑TTG‑ACAGGAGCAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGGCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTggag                                                                    <  2:698661/239‑1 (MQ=255)

                                                                                                                                                                          ttCCGGGTTAATCCCCTTCGGAAGTGCGGGCCCGGACTCC‑TTT‑ACAGGGACAGCGTTATCGGCGTTAATGGTGACGATAATCTCTGTACAGTGCCCCTCGAGGCCTTCGTCGATAGCGTTAGCTACCGCCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGCCTTTGATACTGGAGGAGt                                                                <  2:836142/239‑1 (MQ=255)

                                                                                                                                                                          gccaggttGAATTCCGGTCGGAATGCCCCCGCCGTCAACC‑TGT‑ACAGAGACAGAGTTATCGGCTGGAATGGTGACGATAATTTCTTTACAGGGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTggagga                                                                  <  2:339940/230‑1 (MQ=255)

                                                                                                                                                                             cGGGTTAATCCCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGGTATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCAt                                                             <  1:568964/239‑1 (MQ=255)

                                                                                                                                                                                gTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGat                                                                                                                              <  1:248296/171‑1 (MQ=255)

                                                                                                                                                                                    aTACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGCGACAGCGTTATCGGCGTGAACGGGGACGATAATTTCTTTACAAGGACCCCCCAGCGCTTCGTCGATATCGTTATCTACCACCTCGTATACCATGTGGTGCCGAGCGGGGACGCCTTCCGTGTCGCCGATTTACATACCCGGTCGCCTACTGCCCGCCCCCACCCCTTTCACGAACTTTAAACTCGAGGAGtacaaaaaaat                                                      >  2:370449/1‑229 (MQ=255)

                                                                                                                                                                                    aTACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGGATGGGGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCCTCCATAACGTTATCTCCCACCTCGAGAAACATGTGGTGCAGACCGGCGCCGTCATCCGGTGCGCCGACATCCAAATCCTGGCGCTTCGCCCCCGCACCCATCCCTTTCGCAACTTTGTAACTGGAGGGGCCACAAGaaat                                                      >  2:266624/1‑237 (MQ=255)

                                                                                                                                                                                    aTACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAAtt                                                      <  1:168495/239‑1 (MQ=255)

                                                                                                                                                                                     tACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTc                                                     <  1:579498/239‑1 (MQ=255)

                                                                                                                                                                                         gggtggAATGCCGCGCCCGTCATCCTTGT‑A‑AGGGAACGAGTTATCGGCGTGAATGGGTACGATAATTTCCTTCCAGTGCCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACa                                                 <  2:714792/235‑1 (MQ=255)

                                                                                                                                                                                          gTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATa                                                                                                                                                                      <  1:168085/121‑1 (MQ=255)

                                                                                                                                                                                          gTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCCCATCCAGCCCTTTCCGGACTTTGATCCTGGAGGAGTCCAAAGAATTCGACAt                                                >  1:412792/1‑239 (MQ=255)

                                                                                                                                                                                          gTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCCGGCGCTTACGCACCGCATCCCGCCCTTTCAGGACTTTGATATTGGCGGAGCCATAAGaa                                                        >  1:339940/1‑231 (MQ=255)

                                                                                                                                                                                          gTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGGGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATa                                                                                                                                                                      >  2:168085/1‑121 (MQ=255)

                                                                                                                                                                                              gAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGGATGGGGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCCTTTGGGGCAGACCGGTGCCCCCCTCCCTGTCGCCGCGATACATACCCCGGCGCTTACGCACCCCCTCCCCCCCCTTCCCGACCTTTATACTTGGGGGGTCAAAAGAAtttaaacacaaa                                            >  2:1011147/1‑229 (MQ=255)

                                                                                                                                                                                               aaTGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATaa                                                                                                                                                                                                                               <  2:213618/59‑1 (MQ=255)

                                                                                                                                                                                               aaTGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATaa                                                                                                                                                                                                                               >  1:213618/1‑59 (MQ=255)

                                                                                                                                                                                               aaTGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGGGGAGTCCTAAGAATTCGACATCaaag                                           >  1:714792/1‑237 (MQ=255)

                                                                                                                                                                                                     gcgcCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGGGGCCTAAGAATTCGACATCAACGttttc                                      >  1:908296/1‑236 (MQ=255)

                                                                                                                                                                                                      ccccctttaccc‑TGT‑ACAGAGACAGAGTTTTCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTATGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                                    <  2:412792/229‑1 (MQ=255)

                                                                                                                                                                                                             cATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCAtt                              <  1:20228/238‑1 (MQ=255)

                                                                                                                                                                                                                 c‑TGT‑ACAGAGACAGAGTTATCGGTGTGAATGGTGACGATAATTTCTTTACCGTGACCCGCGGGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATAc                         <  1:309357/239‑1 (MQ=255)

                                                                                                                                                                                                                  cttt‑ACAGAGACAGAGTTATCGGTGTTAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCCACCCCCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATAc                         <  1:698734/236‑1 (MQ=255)

                                                                                                                                                                                                                   tGT‑ACAGAGACAGAGTTACCGGCGTTAATGGGGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACt                        <  1:726933/239‑1 (MQ=255)

                                                                                                                                                                                                                     t‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATaa                                                                                                                                                                                                                               <  1:269645/38‑1 (MQ=255)

                                                                                                                                                                                                                           agacagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCg                                                                                                                                >  1:500456/1‑128 (MQ=255)

                                                                                                                                                                                                                           agacagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCg                                                                                                                                <  2:500456/128‑1 (MQ=255)

                                                                                                                                                                                                                           agacagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTAAGCACCGCATCCAGCCCTTTCAGGACCTTGATACTGGAGGAGGCATAAGAATTCGACATCAACGTTTTTTGCTCATTTTTACTTTGGTTa                 >  1:411566/1‑239 (MQ=255)

                                                                                                                                                                                                                           agacagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCCTTATACAAACCCGGGCGCTTACGACCCGCCTCCACCCCTTTCAGGGCTTTGTTACTGGGGGAGGCATAAGAATTCGACCTCAACCTTTCTCCCTCATTTTTCTCTGGGTTa                 >  2:521872/1‑239 (MQ=255)

                                                                                                                                                                                                                             acagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTTCGCACCGCATCCAGCCCTTTCAGGACTTTGATACTTGAGGAGGCATAAGAATTCGACATCAACGATTCCCGCCCCTTTATACTTGGGTTaa                >  1:535529/1‑238 (MQ=255)

                                                                                                                                                                                                                                gagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCCGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTTTCGCTCATTTATACTTGGGTTAATCCg            >  1:999033/1‑239 (MQ=255)

                                                                                                                                                                                                                                  gTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGGCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCg            <  1:498538/237‑1 (MQ=255)

                                                                                                                                                                                                                                     gtCGGCGTTAATGGTTACGATAATTTCTTTACAGTGGCCCGCGAGCGGTTCGTCGATAGCGTTATCTACCGCCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTAtt       <  1:691132/238‑1 (MQ=255)

                                                                                                                                                                                                                                           gTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGTTACTGGAGGAGGCATAAGAATTCGACATCACCGTTTCCCGCCCATTTTTACTTGGGTTAATCCGTTAtttttc   >  1:586584/1‑235 (MQ=255)

                                                                                                                                                                                                                                           gTGAATGGTGACGATAATTTCTTTACAGGGCCCCGCGAGCGCTTCGGCGATAGCGTTATCTACCCCCTCGAATACCATGTGGTGCAGACCGGGGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTAcc  <  2:999033/238‑1 (MQ=255)

                                                                                                                                                                                                                                           gTGAATGGTGACGATAATTTCTTTACAGGGACCCGCGAGCGCTTCGTCGATCGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGTTTCCGTCATCCGTGTCCCCGATATACATCCCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACATTGTAACTGGGGGTTTATAAAGAATTCGACAGCCCCCTTTTTCTCCCATTTTACCTTGTGGTAATACGTttttttcc   >  2:691132/1‑235 (MQ=255)

GAATTTTACCCTCGCGCTGGATAACCAGCTCCA‑GTTTTTGCGACAGGGCGTTTACT‑ACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTAT‑AGGAGTTATCGTCAAA‑TTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGCCGCCGATACGCCCTCTTCCGGGTGAATACCGGTCGGAATGCCGCGCCCGTCATCC‑TGT‑ACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACC  >  NC_000913/3879699‑3880165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A