Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F113 I1 R1	17	62.1	2245352	90.5%	2032043	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,415,807	A→C	D354A (GAT→GCT)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,415,807	0	A	C	98.2%	159.9 / NA	55	D354A (GAT→GCT)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (33/20); total (34/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAAC  >  NC_000913/2415667‑2415941

cGGTTACGAAATGGACGCGCGCATTTCTAAACTATGAGAACGCACTGTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTGCGGTTGACGCTc                                                                                                                                      >  1:767276/1‑143 (MQ=255)

 ggTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCa                                                                                                                                     >  1:578783/1‑143 (MQ=255)

  gTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCAc                                                                                                                                    >  2:825005/1‑143 (MQ=255)

    tACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCCACCTGGAAGTTCCGGTTGACGCTCACgc                                                                                                                                  >  1:335134/1‑142 (MQ=255)

           tGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATc                                                                                                                           >  1:794360/1‑143 (MQ=255)

              aCGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGCTCCGGTTGACGCTCACGAACGTATCcag                                                                                                                        >  2:617559/1‑143 (MQ=255)

                gcgcgcATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACAAACAACTGGCAGACCTCTCTGAGCCTGCAGAGCGTCAACATGGAAGTTGCGGTTGACGATCGCGAACGGATcacggc                                                                                                                      >  2:894820/1‑138 (MQ=255)

                 cgcgcgTTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTAAGTATTGTGTACTCCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGaaa                                                                                                                     <  2:127586/143‑1 (MQ=255)

                 cgcgcATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGaaa                                                                                                                     <  1:328680/143‑1 (MQ=255)

                 cgcgcATTGCTAAACTGTGCGAACGTGCTTTCGCTGCCGGCCTGCCGGTATTTATGGTGAACACCAACACCGGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGaaa                                                                                                                     <  1:994671/143‑1 (MQ=255)

                      ctttCTAAAGTGTGCGCACGTGCTTTCGCTACCGGACTGCCGGTCTTTGTGGTGAACCCCACCACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTACACCAGTGCCAAGCAATTGACGCTCACGAACGTATCGAGAAAGTTCa                                                                                                                <  2:676013/142‑1 (MQ=255)

                      aTTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGACCGTATCGAGAAAGTTCa                                                                                                                >  1:1015224/1‑143 (MQ=255)

                      aTTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCa                                                                                                                <  1:574431/143‑1 (MQ=255)

                       ttacTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAg                                                                                                               <  1:294698/140‑1 (MQ=255)

                        ttCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCTGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGCTCAgg                                                                                                              >  2:820526/1‑143 (MQ=255)

                           tAAACTGTGCGAACGTGCTTTCGCTACCGACCTGCCGGTATGTGTTGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAAt                                                                                                           <  1:1056236/143‑1 (MQ=255)

                                  tgCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCCACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGACCGTTCAGGAATACggcgc                                                                                                    >  2:406949/1‑139 (MQ=255)

                                   gCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGACCGTATCGAGAAAGTGCAGGAATACGTTGCt                                                                                                   >  1:1015683/1‑143 (MQ=255)

                                     gAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGACGCTCCTGTTGACGCTCACGACCGTATCGAGAAAGTTCAGGAATACGTTGATaa                                                                                                 >  2:145634/1‑143 (MQ=255)

                                     gAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa                                                                                                 >  1:786796/1‑143 (MQ=255)

                                     gAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTACCGGTTGACGCTCACGAACGTATCGAGAAAGTTGAGGAATACGGTGCTaa                                                                                                 >  2:557654/1‑143 (MQ=255)

                                                 gCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCACTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCACACGAACGTATCGAGAAAGATCAGGAATACGCTACTAACTACAACAACGc                                                                                     >  1:11351/1‑143 (MQ=255)

                                                   tACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTg                                                                                   >  1:578560/1‑143 (MQ=255)

                                                          cTGCCGGTGGTTGTGGTGACCACGCCCACCTGGCAGACCTCTCTGAGCCGGTAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt                                                                            <  2:705394/143‑1 (MQ=255)

                                                           tGCCTGTATTTATGGTGACCACCAACACCTGGCAGACCTCTGTGAGCCTGCAGAGCTTCAACCTGGAATTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATc                                                                           <  2:86635/143‑1 (MQ=255)

                                                             ccGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGa                                                                         >  1:888151/1‑143 (MQ=255)

                                                              cGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGAGTGGATCgga                                                                        >  1:237434/1‑141 (MQ=255)

                                                                gTATTTATGGTGACCACCAACACCTGCCAGCCCTCGCTTAGCCTGCATGGCTTCAACCTTGAAGTTCCCGTTGACGCTCACTAACGTATCGAGATAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTCGATCGAAtc                                                                      <  2:875897/143‑1 (MQ=255)

                                                                   tttATGGTGACCACCAACACCTGGCAGATCTCTCTGAGCCTGCAGAGCTTCAACCTGGCAGCTCCGGTTGACGTTCACGTACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATctct                                                                   <  1:238853/143‑1 (MQ=255)

                                                                     gacggTGAACGCCAACGCCTGGCAGACCTCGCTCAGCCTGCAGAGCTTCAACCTGGAAGGTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGa                                                                 <  1:62783/140‑1 (MQ=255)

                                                                      aTGGTGAACACCAACCCCTGGCAGCCCTCTCTGGGCCTGCAGAGCGTCGACCGGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGAc                                                                <  2:969346/143‑1 (MQ=255)

                                                                            cacaccaacgccGGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTTCATCAACGCTGACTGGATCGAATCTCTGACTGCCAc                                                          <  1:722369/142‑1 (MQ=255)

                                                                            aacaccaacaccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTACCTACCTCCACGCTGCCTGGATCGAATCGCTGACTGCCAc                                                          >  2:346485/1‑143 (MQ=255)

                                                                              caccaacaccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGCAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACACCAACGCTGACTGGATCGAAGCTCTGACTGCCACtt                                                        >  2:955069/1‑143 (MQ=255)

                                                                                 caacaccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTg                                                     >  1:730710/1‑143 (MQ=255)

                                                                                       cTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCa                                               >  1:584068/1‑143 (MQ=255)

                                                                                        tGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGGTCCGGTTGACACTCACGAACGTATCGAGCAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGAGCAg                                              >  1:736095/1‑143 (MQ=255)

                                                                                              aCCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtcg                                        >  1:188455/1‑143 (MQ=255)

                                                                                                ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGAGTGCCACTTCTGGGCGCCGCCgccgtc                                      >  1:863269/1‑143 (MQ=255)

                                                                                                    ctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgtc                                  >  1:296122/1‑143 (MQ=255)

                                                                                                    ctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGACACTTCTGAGCGCAGCCGTCgtctgtc                                  >  1:296458/1‑143 (MQ=255)

                                                                                                            gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgt                                          <  2:41823/127‑1 (MQ=255)

                                                                                                            gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgt                                          >  1:41823/1‑127 (MQ=255)

                                                                                                            gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTg                          >  1:847796/1‑143 (MQ=255)

                                                                                                            gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTg                          >  1:708411/1‑143 (MQ=255)

                                                                                                              agagCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAGCGTAGCGAGAAAGTTCAGGAATACGTTGCTCACCACGTCAACGCTGACTGGATCGCAGCTCTGACTGCCACTGCGGAGCGCGGCCGTCGCCCGTCCCCGCCcgcg                        >  2:909513/1‑143 (MQ=255)

                                                                                                                  cTTTAACCTGGAAGTTCCGGTTGACGCACACGACCGTATCGAGAAAGGTCAGGAAGACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTcc                    <  1:1036912/143‑1 (MQ=255)

                                                                                                                  cTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACCTCAACGCTGACTGGATCGAATCTCTGCCTGCCCCTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTcc                    >  1:662869/1‑143 (MQ=255)

                                                                                                                      aaCCTGGAAGTTCCGGTTGACGCTCACGCACGTATCGAGACAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTa                <  1:474943/143‑1 (MQ=255)

                                                                                                                         cTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCa             >  2:816573/1‑143 (MQ=255)

                                                                                                                            gAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTCTCAGct          >  1:1050975/1‑143 (MQ=255)

                                                                                                                            gAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGct          <  1:956485/143‑1 (MQ=255)

                                                                                                                               gtgccGGTTGACGCGCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACGGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGctgac       <  1:145634/140‑1 (MQ=255)

                                                                                                                                   cGGTTGACGCTCACGACCGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAGCGAATCTCTGCCGGCCACCTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGaa   <  1:67936/143‑1 (MQ=255)

                                                                                                                                    ggTGGACGCTCACGAACGTACCGAGAAAGTTCAGGAATGCGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTCACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAAc  <  1:581488/143‑1 (MQ=255)

CGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAAC  >  NC_000913/2415667‑2415941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A