Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F108 I1 R1 17 38.6 1469306 86.0% 1263603 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,414,037 A→C T190P (ACC→CCC)  ackA → acetate kinase A and propionate kinase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,414,0370AC97.8% 123.6 / ‑6.6 45T190P (ACC→CCC) ackAacetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (20/24);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGTATTTGACACCGCGTTCCACCAGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTAACCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGA  >  NC_000913/2413907‑2414166
                                                                                                                                  |                                                                                                                                 
cgCTGTTTTTGACACCGCGTTCAACCATACGATGCCGGAAGAGGCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCgg                                                                                                                       <  2:383477/142‑1 (MQ=255)
   tGTATTTGACACCGCGTTCCACCAGACTATGCCGGAAGAGTCTTACCTCTACGCCCTCCCTTACAACCTGTACAAAGAGCACGGCATCCATCGTTACGGCGCGCACGGCACCAGCCCCCTCTATGTACCCCAGGCAGCCGCac                                                                                                                    >  2:179228/1‑142 (MQ=255)
        ttGACACCGCGTTCCACCAGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATg                                                                                                               <  1:635498/143‑1 (MQ=255)
          gACATCGCGTTCCACCATACTATGCCGTAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCt                                                                                                             <  2:490419/143‑1 (MQ=255)
           acacCGCGTTCCACCAGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTg                                                                                                            <  2:450476/143‑1 (MQ=255)
             acCGCGTTCCACCAGACTATGCCGGAAGAATCTTACATCTACGCCCTGCCTTACAACCTGTACAAAGAGCACAGCATCCGTCGTTACAGCGCGCACGTCACCAGACACTTCTATGTACCCCAGAAAGCGGCAAAAATGCTaaa                                                                                                          >  2:288608/1‑143 (MQ=255)
                 tgTTCCATCAGTCTATGTCTTAATAGTTTTTTCTCTACTTCCTGTTTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACaaa                                                                                                      <  1:543782/142‑1 (MQ=255)
                 cgTCCCACCAGACTATGCCGGAAGCGTCTTACCTCTACGCCCCGCCTGACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCATGAAGCGGCAAAAATGCTGAACaaa                                                                                                      <  1:690345/143‑1 (MQ=255)
                     ccatcaGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCgg                                                                                                  <  1:72846/143‑1 (MQ=255)
                     ccaccaTACTATGCCGCAACACTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCgg                                                                                                  <  1:712874/143‑1 (MQ=255)
                     ccaccaGACTAGGCCGTAGGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCgg                                                                                                  <  2:199747/143‑1 (MQ=255)
                       accaGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGAAATAAGTAATAACGGCGCGCACGGCACCAGCCACTTCTATGTACACCAGGAAGCGGCAAAAATGCTGAACAGACCGGTa                                                                                                >  1:111376/1‑143 (MQ=255)
                         caGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCCCCAGCCCCTTCTATGTACCCCAGGACACGGCAAAAATGCTGAAAAAACCGGAaga                                                                                              >  1:660557/1‑143 (MQ=255)
                         caGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACCTCTATGTACCCCCGGACGCGGCGACAGTGCTGAACAAACCGGTaaa                                                                                              >  1:289650/1‑141 (MQ=255)
                         caGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGAGCGCACGGCACCAGCAACTACTATGTACCCCAGGAAGCGGCAAAAATGCAGAACAAAACGGTaga                                                                                              >  1:367458/1‑143 (MQ=255)
                              tgtGCCGTAAGAGTCGTACCTCTGCGGCCTGCCTTACAACCTGTGCAAAGAGCACGGCATCCGTCGTTACGGCGCCCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAGATGCTGAACAAACCGGTAGAAGAAc                                                                                         <  2:555262/141‑1 (MQ=255)
                              tATGCCGGAAGAGTCTTACCACTACGCCCTGCCTTACAACCTGTAAAAAGAGCACGGCATCCGTCGTTACGGAGCGCACGGCACCAGCCACTTCTATGTACCCCAGGACGCGACAAAGATGCGGAAAAAACCGCACGAAGAAc                                                                                         >  2:303679/1‑143 (MQ=255)
                                tGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTg                                                                                       <  1:295116/143‑1 (MQ=255)
                                 gtcagAAGAGTCTTACCTCTACGCCCTGCCGTACAACCTGGACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGa                                                                                      <  1:432731/139‑1 (MQ=255)
                                     gAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGCATTTAGAACAAACTAATAGAAGGGCACCGGCCACATCTATGTACCCCAGGAAGCGGCAAAAAGGCTGAACAAACACGTAGAAAAACTGAAcac                                                                                  >  2:516188/1‑142 (MQ=255)
                                          gTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGCCCCCAGCCACTTCTATGTACCCCAGGAAACAACAAAAATACAGAACAAAAAGATAGAAGAACTGAACAtcatca                                                                             >  1:87364/1‑143 (MQ=255)
                                            cTTACCTCTACGCCCTGCCTTACTACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACCGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCAcc                                                                           <  2:526579/143‑1 (MQ=255)
                                             ttACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCCTTCCGGCGCGCACGCCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGATGAACAAAACGGTAGAAGAACCGAACAACATCAccc                                                                          >  2:83173/1‑142 (MQ=255)
                                                      cGCCCTGCCTTACTACCTGTCCAAATAGCACAGTATTCTTTTTTACGTCGCGCACGGCACCAGCCACTTATATGGACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTgg                                                                 <  1:602378/143‑1 (MQ=255)
                                                      cGCCCTGCCGTACAACCTGTAGAAGGAGCAAGGCAGCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTgg                                                                 <  1:563182/143‑1 (MQ=255)
                                                          cTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCGGAACAAACCGGTAGAAGAACTGAACATCATCACCTGACACCCgggagc                                                             >  2:358815/1‑140 (MQ=255)
                                                          cTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTAAGTACCCCAGGAAGCGGCACAAATGCTGAACAACCAGGTAGCAGAGCTGAACACCATCACCAGCCAccacgtcaa                                                             >  2:358819/1‑136 (MQ=255)
                                                           tGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACCTCTACGTACCCCAGGAAGCGGCACAAATGCTGAACAAACCGATAGAAGAACTGAACATCATAACCTGCCACCAGAGCAAc                                                            >  1:581883/1‑143 (MQ=255)
                                                              cTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAACCATGCTGAACAAACCGGTAGAAGCACTGAACATCATCACCTGCCACCTGGGCAACggc                                                         >  2:34022/1‑142 (MQ=255)
                                                               ttACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCAGCAGCCACTTCTATGTACCCCAGGAAGCGGCAAACATGCTGAACAACCCGGTCGAACAACTGCACATCATCCACTGCCACCTGTGCACTggtg                                                        >  1:589538/1‑143 (MQ=255)
                                                                 aCAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACggtggt                                                      <  1:156586/143‑1 (MQ=255)
                                                                             aaGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCCCCTGGGCAACGGCGGCTCCGTTTCTGCt                                          >  2:454611/1‑143 (MQ=255)
                                                                             aaGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGc                                                               <  1:701649/122‑1 (MQ=255)
                                                                             aaGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGc                                                               >  2:701649/1‑122 (MQ=255)
                                                                                ggCACGTCATCCGTCGTTACCGCGCGCACGGCCCCAGCCACTTCTATGTAACCCAGGAAGCGGCAAAAATGCTGAACAAGCCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATc                                       <  2:60189/142‑1 (MQ=255)
                                                                                agCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGCGCAACGGCCGTTCCGTTTCTggcata                                       >  2:64505/1‑138 (MQ=255)
                                                                                 gCACGGCCTCCGTCGTTACGGGGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATcc                                      <  2:111376/143‑1 (MQ=255)
                                                                                      gCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTAGGCAACGGAGGATCCGTATCTGCTATCCGCAAc                                 >  1:687496/1‑143 (MQ=255)
                                                                                           cgtggtTACGGCGCGTACGGCACCTGCGGCTTCTATGTACCCCAGGAAGCGGCAAAAGTGCTGAACAAGCCGGTAGAAGAACTGAACATCAGCATCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTaa                            <  2:351157/143‑1 (MQ=255)
                                                                                            gtagtTACGGCGCGCACGCAACCTGCCACATCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTaaa                           <  2:596332/140‑1 (MQ=255)
                                                                                                  aCGGCGCGCACGGCACCAGCCACTTCTATGTACCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGCAGAACTGAACATCCTCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCCGCTATCCGCAACGGTCAATGCGtc                     >  2:124972/1‑142 (MQ=255)
                                                                                                               cACCAGCCACTTTTATGTACCCCAGGAAGCGTCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATgg        <  1:316010/143‑1 (MQ=255)
                                                                                                                 ccAGCCACTTCTATGTGCCCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGc      <  2:660557/143‑1 (MQ=255)
                                                                                                                 ccAGCCACGTCTATGTACCCTAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGc      <  2:367458/143‑1 (MQ=255)
                                                                                                                     ccACTTCTATGGTCCCCAGGAAGCGGCAAAAATGCTGAACAAACCTGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGa  <  2:366522/143‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                 
TGCTGTATTTGACACCGCGTTCCACCAGACTATGCCGGAAGAGTCTTACCTCTACGCCCTGCCTTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTTCTATGTAACCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGA  >  NC_000913/2413907‑2414166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A