Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F110 I2 R1
|
19 |
56.9 |
2036372 |
91.3% |
1859207 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,624,957 |
G→T |
A194S (GCG→TCG) |
yhhI → |
putative transposase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,624,957 | 0 | G | T | 97.4%
| 87.9
/ NA
| 40 | A194S (GCG→TCG) | yhhI | putative transposase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (20/18); total (20/20) |
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATT > NC_000913/3624841‑3625094
|
tCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACACAGGGGCGGCta > 2:311056/1‑143 (MQ=17)
tAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAACAGGAACCCAGGGGCGGCTAAATAAAGCCTTTg > 1:253762/1‑143 (MQ=17)
cTGGATATTAAAGGAAAAATCATCACAACTGATGCGCTGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaa > 2:355706/1‑143 (MQ=17)
gATAATAAAGGAAAAGTCATCAAAACAGATGAGAAGGATTGCGAAAAAGTTATTGCAGAGAAAAAACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAtt < 2:338317/143‑1 (MQ=11)
tAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAACAGGAACCCAGGGGCGGCCAAATCAAGCCTTTGAGGAAAAATTTCCGc > 1:930478/1‑143 (MQ=255)
tAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGc > 2:427775/1‑143 (MQ=17)
ggAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGaa > 1:499912/1‑143 (MQ=17)
gAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGaaa > 1:15401/1‑143 (MQ=17)
gAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGaaa < 1:243903/143‑1 (MQ=17)
gAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACACCGGGCCACCCAAAGAAAGCCTTTGAGGAAAAATTTCCGCTGaaa > 2:208692/1‑143 (MQ=11)
aaaaTCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGa < 1:668545/143‑1 (MQ=17)
atcctaacaacaGATGCGATAGGATGAGAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAAtt < 2:90573/137‑1 (MQ=11)
catcaCACCTGATGCGATGGGATGTCAGAATAATATTGCAGAAAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGTAACCCAGTGGCGGCTAAATAAAGTCTTTGAGGAAAAATTTCCGCTGAAAGAATTaa < 2:141974/143‑1 (MQ=11)
tcaCAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaat > 1:241262/1‑143 (MQ=17)
caACTGAAGCGAGGGGGTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaataat < 1:335290/143‑1 (MQ=255)
aCTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATcc > 1:707668/1‑143 (MQ=17)
gATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCaga > 2:421944/1‑143 (MQ=18)
aagCGAGGGGATGCCAGAAAGATATGGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCagag < 2:730156/141‑1 (MQ=255)
cGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGTGGAAAAATTTCCGCTTAAAGAATTAAATAATCCAGAGCAt < 1:249868/143‑1 (MQ=255)
cGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCAt > 1:88632/1‑143 (MQ=25)
gggTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACa < 1:100798/143‑1 (MQ=25)
cAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAGATAATCCAGAGCATGACAGTTACGc > 1:371754/1‑143 (MQ=25)
aGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCa > 2:470830/1‑143 (MQ=25)
aGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCa < 1:355706/143‑1 (MQ=38)
tatTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAACTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTg < 1:811876/143‑1 (MQ=255)
ttGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACACAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGaa < 1:848917/143‑1 (MQ=255)
tGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCTCGGTAATATGATCCTAGGTGCGTCTATATTAAGGCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGaaa < 1:831094/143‑1 (MQ=11)
ttgagaAGATACAACAAGAGGGAGGTGATGCTTTATGCGAGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCTCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAag < 1:673606/141‑1 (MQ=11)
aagaTACAATAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCCGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCa > 2:643948/1‑143 (MQ=255)
aagaTACAAAAACAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCa < 1:606434/143‑1 (MQ=38)
aagaTACAAAAACAGGGAGGCGACTATTTATTCTCGGTAAAAGGAACGCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAACTTTCCGCTGACAGAATGAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCa < 1:482204/143‑1 (MQ=11)
aaaaaCAGGGAGGTGATTATTTATTATCGGCAAAAGGGACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCaga > 1:697488/1‑143 (MQ=255)
aaaaCAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCagag < 2:460151/143‑1 (MQ=38)
aaaCAGGGAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAgaga > 2:628699/1‑143 (MQ=255)
gAGGTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTGACGGCAGAGAAGaaacc > 2:573811/1‑141 (MQ=255)
gTGATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGGTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGt < 1:531879/143‑1 (MQ=255)
tGATAATTTATTTACGACAAAAGAAACCCAAGGGCAGCTAAATAAAGTCTTTGAGGAAAAATTTCCGATGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTc < 2:462598/143‑1 (MQ=11)
gATTATTTATTCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGGAGTATATAATCCAGAGCATGACAGGTACGCAATCAGTGAAAAGAGTCACGGCAGAGGAGAAATCGGtgt > 1:38168/1‑141 (MQ=11)
ttattCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCatat > 2:286921/1‑143 (MQ=255)
tattCTCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATAtt < 1:39561/143‑1 (MQ=38)
|
TCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATT > NC_000913/3624841‑3625094
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
