Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I0 R1 43 55.9 1320120 86.3% 1139263 218.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,244,539 A→G 20.2% N179N (AAT→AAC)  malF ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,5390AG20.2% 83.2 / 2.1 46N179N (AAT→AAC) malFmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (11/24);  new base G (7/3);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 6.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTC  >  NC_000913/4244315‑4244757
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 
ccGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCGCTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGAtt                                                                                                                                                                                                                                 <  1:398536/227‑1 (MQ=255)
         aGCTTTTCATCCCCCCAATTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATc                                                                                                                                                                                                                       <  1:243809/228‑1 (MQ=255)
                                                  gTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TTGCACTCCACGCCCTACGGTTTTTGGGAAACACCGCCCGG‑ATTCGCGCGTCGGCCTCGGGGTTgggggtccg                                                                                                                                                                             >  1:119630/1‑222 (MQ=255)
                                                                               atTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGGTGCCCTCCGGCAGAATGGCGGGAA‑TGTCACACCGCGACCGTCGGGTTTGGGTAATTA‑CCTGCCGAATTCTCG‑GTCCGCCCTCCGGCTGGGCGGCGCTCTC‑TTTTCGTTTCA‑Gttctttttgctct                                                                                                                                              >  2:254145/1‑219 (MQ=255)
                                                                                tttCGCGCCGGTTTGGTTTTTCGGCAACGTGCCGTCTCCGTCGAGTGTGTAGAGCGGCTGGGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑gccgcc                                                                                                                                           <  1:325467/228‑1 (MQ=255)
                                                                                        tcgtctGGTTATTGGTCAACGTGCCGTCACCGGCGGGTGTGTAGAGCGGTTGCGTGCCAGAAAACTGGGGGAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTaa                                                                                                                                   <  2:162197/225‑1 (MQ=255)
                                                                                         ggtctGTTTTTTCGTCAACGTGCCGTCACCTTAGAGTGTGGAGAGCGGCTGCGTGCCAGAAAAATGGCGCCGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTaa                                                                                                                                   <  2:309617/225‑1 (MQ=255)
                                                                                         ggtcggATTATGCGGCAACGTGCCGTCCCCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTaa                                                                                                                                   <  1:609507/222‑1 (MQ=255)
                                                                                           tctGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTcgcgcg                                                                                                                                                                                                    <  1:347903/163‑1 (MQ=255)
                                                                                               attattCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGGGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGGCGTGTC‑TTTTAGTTGCC‑GTTTTTGCTT‑GTCGCCCACTTTtaaacggcc                                                                                                                            >  1:471101/1‑223 (MQ=255)
                                                                                                    tCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGGAA‑TGTCCCTCAGCGCCTCACGATTTTGGGGAAACC‑CGGGCGG‑ATTTGAGCGTTACCCCTCGGGCTGGGGGGGCGGTTC‑TTTCTGTTTGC‑GTTTTTTCTCTGCCCCCAACTTTAAAAggccggggg                                                                                                                        >  2:409552/1‑220 (MQ=255)
                                                                                                    tCGTCAACGTGCCGTCACCGTCGAGTGGGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCCGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGCAA‑TGGGCATCAGCGGCTGCCGCTTCTTGGTAATCA‑CACCCAG‑GTTTGACCGTTCGCCTCTGGGCTGGGCGGGGGTTTC‑TTTCTGTGGTG‑GGTTTTGTGC‑GCCGCCCAATTTaaaataggcgggg                                                                                                                        >  2:419347/1‑218 (MQ=255)
                                                                                                     cGTCAAGGGCCCGGCACCGGCGAGTGTGTAGAGCGGCTGCGGGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGaga‑g                                                                                                                     <  1:283412/228‑1 (MQ=255)
                                                                                                         aaCGTGCCGTCACCGTCGAGTGTGTAGAGCGGCGGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACCCAAACCCTGACGATTCTCGGGAATCA‑CCCGCAG‑ATTCGCCCGGTCGCCTTCGGGCTGGGGGGGCGTTTC‑TTTCAGTTTCG‑GTGTTTTTTC‑TCCCCCCAATTTAAACACGCCGGGGA‑GGTa‑a                                                                                                                >  2:129894/1‑228 (MQ=255)
                                                                                                           cGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTTGGCGGGCGTTTC‑TTTCCGTTGCA‑GGTTTTGCTC‑GCCCCCCAATTTAAAAGCGTCGGAGA‑GGGa‑act                                                                                                              >  1:169738/1‑226 (MQ=255)
                                                                                                                    aCCGTCGAGTGTGTAGAGCGGCTGGGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGt                                                                                                     <  1:409552/228‑1 (MQ=255)
                                                                                                                       gTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑ATTCCCTCAGCGCCTGACGATTCTGGGGAAACA‑CCCGCCG‑AGTCGCGCGGTCTCCTTCTGGCTGGGGGGGCGTTTC‑TTTCAGTTGCC‑GTTTTTTCTC‑GCCGCCCACTTTTAAAGCGTCGGGGAGGGTA‑TTTTTTGCCGGttt                                                                                                   >  2:546048/1‑228 (MQ=255)
                                                                                                                        tCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGCAA‑AGGCACTCATCGCCTGACGGTTCTGCGTAATCA‑CGCGCCA‑CTTCGGGCGCTGGCCTTCGGGCCGGCGGGTCGTTTC‑TTTCAGTTGCAGGTTTTCGCCC‑CCGCCCACATTTAAAA‑CGCCGAGGG‑GGGA‑AATTTTATCGGTTTCg                                                                                                 >  2:36642/1‑228 (MQ=255)
                                                                                                                             tgtgtAGAGCGGCTGCGTGCCAGAAAACTGGCCCAGGGAGCTCATCATCACTTTGTTGCCATCAGGCAGAATGGCGGGAA‑TGTCACCAAGCGCCCGCCCGTTCTTGGTAAAAA‑CCCCCAG‑GTTAGCCCCTTCGCCGTGCGCGGGGGCGGGCGTTCC‑TTTCAGTTGTA‑GTCTTTTTCC‑GCGGCCCAATATAATAGGGTGGGGGG‑GGGA‑ATTTTTGCCGGTTTTGCCggg                                                                                            >  2:495382/1‑226 (MQ=255)
                                                                                                                               tgtAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAACGCCTGACGAGTCTTGGGAAACC‑CGCGCCG‑ATTCGCGCGGTGGCCCTCTGGGGGGGCGGTCGTTTCTTTTCAGTTCCG‑GTTTTTCCCC‑G‑CGCCACATTTTAAAACGTGCGGGG‑GGTG‑ATTTTTTGCGGTGTCGCCgctgc                                                                                          >  2:470992/1‑224 (MQ=255)
                                                                                                                                     gCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCCCTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAgcg                                                                                    <  1:470992/228‑1 (MQ=255)
                                                                                                                                        gCTGCGTGCCAGAAAACTGGGGCAGGGAGCTTATCATCCCTTTGGTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGcc                                                                                  <  2:119630/227‑1 (MQ=255)
                                                                                                                                         cTGCGTGCCAGAAAACTGGGCCAGGGGGTCCATCATCACTTTGTTGCCATCCGGAAGAATGGCGGTAA‑TGTCCCTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAg                                                                                <  2:147579/228‑1 (MQ=255)
                                                                                                                                         cTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGGTTCTGGGTAATCA‑CCGGCAG‑ATTCGCGCGTTTGCCTTCGGGCTGGGCGGGCGTTTC‑TTTCAGTTTCG‑GTTTTTGTTC‑GCCGCCCCATTTTAAAG‑GTCGCGGAGGGGA‑AATTTTTCCGGTTTTCCCCTCCCCGAgcgcacg                                                                                >  2:93457/1‑225 (MQ=255)
                                                                                                                                         cTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGGCACTCAGCGACCGACGATTCTGGGGCATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCCGGCTGGGCGGTCGTTCT‑TTTCCGATGCA‑GTATTTCCTG‑GCCGCCCAAATTACAAAAGTCGGAGG‑TGGA‑CTTTTTGCAGCTTTCCCCGCCGCAGGGCACCa                                                                                 >  2:341364/1‑227 (MQ=255)
                                                                                                                                         cTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑AGGCACTCAGCGCCTCACGGATCTGGGGAATTA‑CCCGCAG‑ATTCGCGCGTGCCCCTTTCGGCTGGGGGGGCGGTTT‑TTTTAGTTGCA‑TTGTTTGTCC‑GCGCCCACATTTGAAAGCGCTGGTGA‑GGGT‑AATTTTGTCCGTTTTGCCCTcccctaggcgcc                                                                                 >  2:363223/1‑216 (MQ=255)
                                                                                                                                                           gcgcAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑AGTCCCTCAGCGCCCGACGATTCTGGGTAATCA‑CGCGCCG‑ATTCGCCCGTTTCCCTTCCGGCTGGGCGGCCGTTTA‑TTTCCGTTGCA‑GGTTTTTCTC‑TCCGCCCAATTTTAAAGCGGCGCGGG‑GGGA‑ATTTTTGCCGGTTTCGCCGTCTCTGAGCGCCAGTTGCCCCCCATcacccgc                                                              >  2:435027/1‑228 (MQ=255)
                                                                                                                                                            ggcAGGGGGTTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCCCTCGGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCGTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCGC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgcccgc                                                              <  2:437065/226‑1 (MQ=255)
                                                                                                                                                            cgcgGGGAGCTCATCATCTCTTTGTTGCCCTCCGGCAGAATGGCGGTAA‑TGTCGCTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgcccgc                                                              <  1:72352/227‑1 (MQ=255)
                                                                                                                                                                tggtgCTCATCATCAGTTTGGTGCCATCAGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGAGTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTGCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGt                                                         <  1:641745/224‑1 (MQ=255)
                                                                                                                                                                       catcatccCTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCCCTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGAcc                                                  <  1:592803/228‑1 (MQ=255)
                                                                                                                                                                        atcctcaCTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGGTTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGAcc                                                  <  1:234762/227‑1 (MQ=255)
                                                                                                                                                                        atcatccCTTTGTTTCCATTCGGCAGAATGGCGGTAA‑TGGCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGAcc                                                  <  1:163197/227‑1 (MQ=255)
                                                                                                                                                                         tcattaCTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCa                                                 <  1:546048/227‑1 (MQ=255)
                                                                                                                                                                         tcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGGCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCa                                                 <  1:262759/227‑1 (MQ=255)
                                                                                                                                                                               cTTTGTTGCCCTCCGGCAGAATGGCGGTAATTGTC‑CTCAGCGCCTGGCGGTTCTGGGTAATCT‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTta                                           <  1:522038/227‑1 (MQ=255)
                                                                                                                                                                                tttGGTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTtat                                          <  1:5957/227‑1 (MQ=255)
                                                                                                                                                                                  tgttgCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCCG‑ATTCGCGCGGTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGAA‑GTTTTTGCTC‑TCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCCGTTTCCCCTCATCGCCCGCCGGGGAAAAACCAGAGTtatacg                                       >  1:382842/1‑226 (MQ=255)
                                                                                                                                                                                  tgttgCCATCCGGCAGAATGGCGGTAA‑TATCACTCAGCGCCTGACGGTTCTGGGGAATCA‑CCCGCAG‑GTTCGCGCGTTCGCCCTTCGGCGTGGCGGGCGTTTT‑TTTTTGTTGCA‑GTTTTTGGTC‑GCCGCCCCATTTAAAAACGGCAGTGG‑AGTAGATTTTTTCGGTTTTCCCATTCCCTTGACGCCATTTGCACCTACTCGCCCCCCGGGTACAGCCAAAAGtaaa                                          >  2:638550/1‑223 (MQ=255)
                                                                                                                                                                                   gttgCCATCCGGCAGAATGGCGGTAA‑TGTCCCTCAGCGCCTGGCGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTa                                                        <  1:531706/210‑1 (MQ=255)
                                                                                                                                                                                           ccGGGAGAATGGCGGTAA‑TTTCACTCAGCGCCTGACGGTTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTg                              <  1:476264/228‑1 (MQ=255)
                                                                                                                                                                                                          tAA‑TGTCACTCAGCCCCTGACGATTCGGGGTAATCA‑CGCGCAG‑ATTCGTGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACGCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGa                <  1:84658/227‑1 (MQ=255)
                                                                                                                                                                                                               gTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTa            <  1:526079/227‑1 (MQ=255)
                                                                                                                                                                                                                  acccagccacTGACGATGCTGGGTAATCG‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaac          <  1:254145/217‑1 (MQ=255)
                                                                                                                                                                                                                        cgcCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCGGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATTGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCCGCTTGCCCGGAGAGCTCCAACAACACTTc  >  2:271107/1‑228 (MQ=255)
                                                                                                                                                                                                                        cgcCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATCGGTTTTGCCTGCTTGCCAGGAGCGGTCTAACAACACTTc  >  1:46829/1‑228 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 
CCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCA‑CGCGCAG‑ATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTC‑TTTCAGTTGCA‑GTTTTTGCTC‑GCCGCCAAATTTAAAAGCGTCGGAGA‑GGTA‑ATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTC  >  NC_000913/4244315‑4244757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A