Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F27 I0 R1	185	118.8	2757226	85.6%	2360185	223.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	910,811	T→G	22.8%	H80P (CAC→CCC)	poxB ←	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	910,811	0	T	G	22.8%	81.2 / 4.4	53	H80P (CAC→CCC)	poxB	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand): ref base T (17/23); new base G (9/4); total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTT  >  NC_000913/910581‑911046

cAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGTAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACTCGATTCAGGTGGCAATCGAACAGGCCGTCGAT‑TAAGTGCAGGtg                                                                                                                                                                                                                                        >  1:828214/1‑237 (MQ=255)

cAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAAAAGGCCGTTGAT‑TAAGTGAAGGt                                                                                                                                                                                                                                         >  1:1201703/1‑237 (MQ=255)

           ccgcGGTTAAGCCCCGCTTTGCCATTGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAACCCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCa                                                                                                                                                                                                                            <  1:305634/236‑1 (MQ=255)

           ccccGGTTAAGCACCGCTTTGGGAATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGCCTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCa                                                                                                                                                                                                                            <  1:570158/236‑1 (MQ=255)

           ccACGGTTAAGCACCGCTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCa                                                                                                                                                                                                                            <  1:1122712/239‑1 (MQ=255)

                       acccttttGCGCATGGCAATCGCCAGTCTTTGTGGGATTTCCCCTGGGCTGGAAACCAGTTCGTAATAGGGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGGGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑gcg                                                                                                                                                                                                                 <  2:1178228/232‑1 (MQ=255)

                          gtttggccattGGGAACCGCCATTACTTGGGGGATCTGCCCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGAc                                                                                                                                                                                                            <  1:916631/229‑1 (MQ=255)

                           cTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTTCCGGGGCCGCACGATCCG‑GCGCAGAc                                                                                                                                                                                                            >  1:195886/1‑238 (MQ=255)

                           cTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCCCGATCCG‑GCCCAGAc                                                                                                                                                                                                            >  1:1025636/1‑238 (MQ=255)

                           cTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GGGCAGAc                                                                                                                                                                                                            >  1:68069/1‑238 (MQ=255)

                           cTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGAc                                                                                                                                                                                                            >  1:607300/1‑238 (MQ=255)

                           cTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGAc                                                                                                                                                                                                            >  1:489825/1‑238 (MQ=255)

                           cTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGGGCAGGTTGCCGGGGCCGCACGATCCG‑GGCCAGAc                                                                                                                                                                                                            >  1:578938/1‑238 (MQ=255)

                           cTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑ACAGGGCAGGTGGCCGGGGCCGCACGATCAG‑GCGCAAAc                                                                                                                                                                                                            >  1:626204/1‑238 (MQ=255)

                              tGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATCTGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGGGCAGGTTGCCGGGGCCGCACGTACCG‑GCGCAGACcccc                                                                                                                                                                                                        >  2:707770/1‑239 (MQ=255)

                                cgcATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGCTTGCGGCGGCAATCGCACAGGCCGTTGAT‑TAATGGCAGGTTGCCGGGGCAGCACGATCCG‑GCGCAGACCGCCAg                                                                                                                                                                                                      >  1:83642/1‑239 (MQ=255)

                                  gttGGCAGTCGCGTGTATTGGTGGGTTCTGCCCGGGGCGGGAAACCAGCTAGAAATAGTGACTACATTCGCGGAATAGCTTTTGTGGGTGGGTTTCCTGGAAATAGCGGAGGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGtt                                                                                                                                                                                                    <  2:437758/237‑1 (MQ=255)

                                  cATGGCAATCGCTAGTCCTTGTGGGATTTGCTCCGGGCTGGAACCCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTGCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGtt                                                                                                                                                                                                    <  1:372520/239‑1 (MQ=255)

                                   ctGGCAATCGCCAGTACTTGGGGGATCTGCCCCGGGCTGGAACCCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGGGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGtt                                                                                                                                                                                                    <  1:175489/237‑1 (MQ=255)

                                   atggacaTCGCCAGTACTTGGGGGATCTGCTCGGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGGGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGtt                                                                                                                                                                                                    <  1:1371652/232‑1 (MQ=255)

                                   agggCAATCGCCGGGTCTTGTGGGAACTGCCCCGGGCTGGAAACCAGCTCGCAATAGTGACTACAGTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGtt                                                                                                                                                                                                    <  1:837906/236‑1 (MQ=255)

                                   aTGGCAATCGCCAGTACTTGTGGGATCTGCCCCGGGCTGGAACCCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGtt                                                                                                                                                                                                    <  1:707770/238‑1 (MQ=255)

                                     ggCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCCCTGGAGGGAATATGAGCGGAAA‑T‑CCCCCGTCCCGAACCTGGGTTGGGGGGACAACGGAAC‑GGCGCGTGATGGAAATGCAGGTTGCCCGGGCCCGCCGACCCG‑GCGCAGAACGCCaatcggc                                                                                                                                                                                                 >  2:837906/1‑233 (MQ=255)

                                          tCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAACCCAGCTGGCAATAGTGACTACATTCGCGGAATAGCTCTTGGGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTa                                                                                                                                                                                            <  1:541941/239‑1 (MQ=255)

                                                             ttGCCCCGGGGTGGAACCACGCTCGAAATGGGGATTGCATTCGCGAAATAGCTCTTGTGGGTTGGTTTCGGGGAAATGGCCGCTGCCAATGTCGCGGAAGGGAATAGGAGCGGCAA‑T‑CGCCAGTCCCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑Ca                                                                                                                                                                         <  2:644671/237‑1 (MQ=255)

                                                              tGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCCGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑Cacc                                                                                                                                                                       >  1:550534/1‑237 (MQ=255)

                                                                cTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGCGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTCATTTTGGCTTCGGCGc‑cgacg                                                                                                                                                                      >  1:925698/1‑234 (MQ=255)

                                                                cTCAGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACTGGCCGTTGAT‑TAAGGGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCCGCCAGCACGTATTCAGCTAGGTTGGGCTTCAGGGc‑cgacg                                                                                                                                                                      >  2:41758/1‑234 (MQ=255)

                                                                   cGGGCTGGAAACCAGCTCGCAATAGCGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGGGCAGGTTGCCGGGGCCGCACGATCCG‑GGGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCaa                                                                                                                                                                  >  1:701241/1‑239 (MQ=255)

                                                                          gAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTTCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCGAGCACA‑ACCGCCAATTCTCCCCGAACTTTGGCTTCAACGCACAG‑GGCAAAGggcgc                                                                                                                                                           >  2:140565/1‑239 (MQ=255)

                                                                               cAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGGT‑TAAGGGCCGGTTGCCGGGGCCGCCCGATCCG‑GCGCCGACCTCCGGTTCTCCCGTAAGTTGTGTTCCAGCCC‑CACCGGCAAAAGCCCCCAcc                                                                                                                                                       >  2:222733/1‑237 (MQ=255)

                                                                                  cTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAggaccccccgtct                                                                                                                                                    >  1:240051/1‑227 (MQ=255)

                                                                                              aCTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCa                                                                                                                                                                                                                            >  2:948694/1‑156 (MQ=255)

                                                                                              aCTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCa                                                                                                                                                                                                                            <  1:948694/156‑1 (MQ=255)

                                                                                              aCTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGGGGCgggggg                                                                                                                                       >  1:73694/1‑239 (MQ=255)

                                                                                               cTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAGGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCCGTTCTCCGCTAAGTTGGGCTTCACCGC‑AAGGGGCAAAGGCCGCCACTCCTCCGggggggggggg                                                                                                                                      >  2:523538/1‑238 (MQ=255)

                                                                                                   aTTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCACCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGG‑GGGTgtgggct                                                                                                                                  >  1:1112517/1‑232 (MQ=255)

                                                                                                   aTTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGGGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCGGCGAAGTTGGGCTTCAGCGC‑CGGCGGCAAAAGCCCCCACTTCTTCGgggggggggggagc                                                                                                                                   >  2:242008/1‑234 (MQ=255)

                                                                                                     tCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGGGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCTGGGCggggggggacgc                                                                                                                                 >  2:663232/1‑232 (MQ=255)

                                                                                                                ctctTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACCGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGATCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGACGGGGTGACATCCCCTCCgtggtg                                                                                                                      >  1:486058/1‑238 (MQ=255)

                                                                                                                    tgggggtgggtTCCCGGGAAATTGCCGCTGCCATTTCCGTTGGAGGGAATATGGGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCAt                                                                                                                 <  1:66576/236‑1 (MQ=255)

                                                                                                                     gtggggggGTTTTCTGGAAATAGCCCGTGCCAATTGTGGGGGGGGGAAATTGGGGGGGAATT‑CGCACTGACGGGACGGTGGTTGGGGGGGTAATCGAACAGGCCGTTGAT‑TAAGGCCCGGTTGCCGGGGCCGCAGGATCCG‑GCGAAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑AAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCAt                                                                                                                 <  2:1014587/239‑1 (MQ=255)

                                                                                                                                tCCTGGAAATGGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACt                                                                                                     <  1:242008/239‑1 (MQ=255)

                                                                                                                                cccggaaaaTGGCGGCTGCCAATTTCGCTGGAGGGAATATGGGCGGCAA‑T‑CGCCAGTACCGGACGGTGATTGCGGGGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCAt                                                                                                                 <  1:1368767/221‑1 (MQ=255)

                                                                                                                                 tccgtgaaagaccGGTGCCAATTTCCCTGGGGGGAAATTGGGGGGGAA‑TCCGCACGGCCCGGAACTGGATGGGGTGGGCAATGGAACAGGCCGTTGAT‑TAAGTGCAGGTGGCCGGGGCCGCCCGTTCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGT‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACt                                                                                                     <  2:550534/228‑1 (MQ=255)

                                                                                                                                             cggtGCAAATTTCGTTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGGACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGAc                                                                                          <  1:1007576/234‑1 (MQ=255)

                                                                                                                                                 cgcattttCGATGGGGGGAATAGGGGGGGAAA‑T‑CGACGGGCCGGGAAGGGGATGGGGGTGCCAAGCGAAAGGGCCGTTGAT‑TAAGGGCAGGTTGCGGGGCCCGCACGACCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTc                                                                                     <  2:291038/233‑1 (MQ=255)

                                                                                                                                                                 ggaaatggAGCGGCAA‑T‑CGCAAGTACCGGAACGTGATGGGGGTGGCAATCGAAAAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGa                                                                     <  1:918274/231‑1 (MQ=255)

                                                                                                                                                                 gaaaTAGGGGCGCCAC‑T‑CGCAAGTACCGGAACGGTATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGa                                                                     <  1:455742/236‑1 (MQ=255)

                                                                                                                                                                   aaTAGGAGCGGCAA‑T‑CGCCATTACCGGACCGTGATGGCGGTGCAAATCGAGCAGGCCGTTGAT‑TAAGTGCAGGTGTCCGGGCCCGCACGAGCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGGGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGa                                                                                                                           <  1:668625/182‑1 (MQ=255)

                                                                                                                                                                                            ccGGAATGTTGTTGGGGGGGCAATCGACCCGGCCGTTGAT‑TAGGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGcc                                           <  1:523538/239‑1 (MQ=255)

                                                                                                                                                                                                   gttgttGCGGTGCCAATGAACCAGGCGTTGGAT‑TGAGGGGCGGTCGCGGGGCCCGCACTGCCCG‑GCGCAGCCCGCCAGTCCTCCGCTAGGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGa                                    <  2:195886/235‑1 (MQ=255)

                                                                                                                                                                                                                                      aaGGGCAGGTTGCGGGGGCCGCACGACCCG‑GCCCAGCCCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCgtttgttt  <  1:663232/239‑1 (MQ=255)

CAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCAATCGCCAGTACTTGTGGGATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAA‑T‑CGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGAT‑TAAGTGCAGGTTGCCGGGGCCGCACGATCCG‑GCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGC‑CAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTT  >  NC_000913/910581‑911046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A