Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A69 F2000 I2 R1 32 130.0 2789900 93.6% 2611346 220.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007779_cfb 547,694 A→G intergenic (+123/‑1156) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007779_cfb547,6940AG99.2% 336.7 / NA 121intergenic (+123/‑1156)fdrA/ylbFacyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (61/59);  total (61/60)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 124 total aligned reads displayed.

ATCGGATTACGCAGCTTTGCGCTGGAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACG  >  NC_007779_cfb/547449‑547937
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
aTCGGATTACGCAGCTTTGCGCTGGAGCTACAAAGCGCTAGCAAACTGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGCGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTCG‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATCCGTCACACTCGTCCACATTGGCTGGATGAGGAACCGGATTCGTCCCATATCAGCGAACTAAGCGAGGGC‑a                                                                                                                                                                                                                                                   >  2:471918/1‑250 (MQ=255)
aTCGGATTACGCAGCTTTGCGCTGGAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑a                                                                                                                                                                                                                                                   <  1:727123/250‑1 (MQ=255)
  cGGATTACGCAGCTTTGCGCTGGAGCTACAAAGCGCCAGCAAACCGGTCGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑aaa                                                                                                                                                                                                                                                 >  1:279935/1‑250 (MQ=255)
       tACGCAGCTTTGCGCTGGAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACt                                                                                                                                                                                                                                            >  1:360932/1‑250 (MQ=255)
            ngCTTNGCGCTGGCGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTc                                                                                                                                                                                                                                       <  2:816178/249‑1 (MQ=255)
               tttGCGCTGGAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACg                                                                                                                                                                                                                                    >  1:1012977/1‑250 (MQ=255)
                     cTGGAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGgc                                                                                                                                                                                                                              >  1:21505/1‑250 (MQ=255)
                       ggAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGgccg                                                                                                                                                                                                                            <  1:805064/250‑1 (MQ=255)
                            tACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCAGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTc                                                                                                                                                                                                                                       <  1:693360/234‑1 (MQ=255)
                            tACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCAGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTc                                                                                                                                                                                                                                       >  2:693360/1‑234 (MQ=255)
                                   cgcCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg                                                                                                                                                                                                                <  1:226795/250‑1 (MQ=255)
                                         cAAACCGATTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAAT‑AAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCCGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga                                                                                                                                                                                                         >  1:163247/1‑250 (MQ=255)
                                          aaaCCGGTTGTGCATTACCAATGCTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga                                                                                                                                                                                                         >  2:901045/1‑250 (MQ=255)
                                                    tgCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc                                                                                                                                                                                               <  2:789950/250‑1 (MQ=255)
                                                        ttACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGTGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGAAGATGACCGGACCCATg                                                                                                                                                                                           >  1:1175471/1‑250 (MQ=255)
                                                            cAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCAAGCCGGGCCGCCAATGCGCTGGCAGGGGATGACCGGACCCNttncag                                                                                                                                                                                       >  2:1179610/1‑245 (MQ=255)
                                                               tGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg                                                                                                                                                                                    <  1:901045/250‑1 (MQ=255)
                                                                   cGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTCCATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATgc                                                                                                                                                                                                                     <  1:580861/213‑1 (MQ=255)
                                                                   cGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTCCATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATgc                                                                                                                                                                                                                     >  2:580861/1‑213 (MQ=255)
                                                                        gTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTgg                                                                                                                                                                           >  1:1007892/1‑250 (MQ=255)
                                                                               gTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATg                                                                                                                                                                    <  1:228172/250‑1 (MQ=255)
                                                                                tGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGt                                                                                                                                                                   <  2:279935/250‑1 (MQ=255)
                                                                                    aaTAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGt                                                                                                                                                               >  1:1254493/1‑250 (MQ=255)
                                                                                         aaanCTNGCTCGTTTATTAGAACGTTTTCAATAAGGAGTTC‑CAATGTTTACAGCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGaa                                                                                                                                                          <  2:1175471/250‑1 (MQ=255)
                                                                                           aaCTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAgg                                                                                                                                                        >  2:693550/1‑250 (MQ=255)
                                                                                            aCTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGt                                                                                                                                                       <  1:501199/250‑1 (MQ=255)
                                                                                                 cgcGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTc                                                                                                                                                                                                                                       <  2:954652/163‑1 (MQ=255)
                                                                                                 cTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTc                                                                                                                                                                                                                                       >  1:954652/1‑165 (MQ=255)
                                                                                                      ttattaGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGgccgc                                                                                                                                                                                                                           >  2:139235/1‑172 (MQ=255)
                                                                                                      ttattaGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGgccgc                                                                                                                                                                                                                           <  1:139235/172‑1 (MQ=255)
                                                                                                      ttattaGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAACGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAg                                                                                                                                             >  2:245922/1‑250 (MQ=255)
                                                                                                         ttaGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgc                                                                                                                                                                                   >  2:791168/1‑209 (MQ=255)
                                                                                                         ttaGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgc                                                                                                                                                                                   <  1:791168/209‑1 (MQ=255)
                                                                                                           aGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAGACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATg                                                                                                                                                                    <  1:862137/222‑1 (MQ=255)
                                                                                                             aaCGTTTGAAATATGGGGTGC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAgc                                                                                                                                      <  2:21505/250‑1 (MQ=255)
                                                                                                                ggttGCAAAAAGGGGTTC‑CCATGTTTACAACAGGGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCa                                                                                                                                   <  2:1007892/248‑1 (MQ=255)
                                                                                                                 tttGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAg                                                                                                                                  >  1:321417/1‑250 (MQ=255)
                                                                                                                 tttGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAg                                                                                                                                  >  1:463611/1‑250 (MQ=255)
                                                                                                                   tGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCa                                                                                                                                                                                                                                      >  2:362258/1‑148 (MQ=255)
                                                                                                                   tGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCa                                                                                                                                                                                                                                      <  1:362258/148‑1 (MQ=255)
                                                                                                                    gCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGAt                                                                                                                                           >  1:138746/1‑238 (MQ=255)
                                                                                                                    gCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGAt                                                                                                                                           <  2:138746/238‑1 (MQ=255)
                                                                                                                         aaGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGc                                                                                                                          >  2:103536/1‑250 (MQ=255)
                                                                                                                            gggTTCACCATGTTTACTGCTGGGGCCAAAGCCAATGCTGCCGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCGCGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCaa                                                                                                                        <  2:163247/250‑1 (MQ=255)
                                                                                                                              cgtacaaaTGTTTCCATAAGTGGGCCAGGCAAAAGATACAGTTATCTAACAAATTCGTCGCGCTCGTCCACTCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACAAAGGA‑AAAACACGGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATa                                                                                                                      <  2:1372246/243‑1 (MQ=255)
                                                                                                                                tC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCCTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCA‑GCGCTGGCAATACTgg                                                                                                                  >  2:762326/1‑250 (MQ=255)
                                                                                                                                 c‑ccATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGcc                                                                                                                                                                                                                                  <  2:385731/138‑1 (MQ=255)
                                                                                                                                 c‑ccATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGcc                                                                                                                                                                                                                                  >  1:385731/1‑138 (MQ=255)
                                                                                                                                    cATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAACGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAg                                                                                                                <  1:245922/250‑1 (MQ=255)
                                                                                                                                     aTGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACCGGAGc                                                                                                               <  1:103536/250‑1 (MQ=255)
                                                                                                                                         ttACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCTGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg                                                                                                          >  2:528375/1‑250 (MQ=255)
                                                                                                                                          tACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAGCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACAGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg‑g                                                                                                        <  2:15443/250‑1 (MQ=255)
                                                                                                                                          tACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg‑g                                                                                                        >  1:1118480/1‑250 (MQ=255)
                                                                                                                                           aCATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg‑gg                                                                                                       <  2:1254493/250‑2 (MQ=255)
                                                                                                                                           aCATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg‑gg                                                                                                       >  1:378142/1‑249 (MQ=255)
                                                                                                                                           aCATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTCCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg‑gg                                                                                                       >  2:1347191/1‑249 (MQ=255)
                                                                                                                                              tCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGCGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGCGCGCATGTCTGTTCGAAGGGTGGGCGAAAGATGAAGCGCAGGCGCTGGCAACACTGGAGCA‑ggg‑ggaag                                                                                                    >  2:221741/1‑246 (MQ=255)
                                                                                                                                                aGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGgcc                                                                                                                                                                                                                             <  2:1118822/129‑1 (MQ=255)
                                                                                                                                                aGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGgcc                                                                                                                                                                                                                             >  1:1118822/1‑129 (MQ=255)
                                                                                                                                                   ggCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg                                                                                                                                                                                      >  2:1328648/1‑166 (MQ=255)
                                                                                                                                                        aaGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGtt                                                                                                                                                      >  2:794688/1‑192 (MQ=255)
                                                                                                                                                        aaGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGtt                                                                                                                                                      <  1:794688/192‑1 (MQ=255)
                                                                                                                                                        aaGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCAt                                                                                           >  1:1393306/1‑250 (MQ=255)
                                                                                                                                                             gaTGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCtt                                                                                      <  1:1200189/249‑1 (MQ=255)
                                                                                                                                                                     ggTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACCTCATTCCTTGTCACCAt                                                                              <  2:378142/250‑1 (MQ=255)
                                                                                                                                                                      gTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACTCTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAtg                                                                             >  1:315863/1‑250 (MQ=255)
                                                                                                                                                                        ttcggANCAAATTCGTCGCGTTCGTCCACACTGGCTGGATGTACAACCGCATTGTGCACTTATCAGCGAGCTAAACGAGGGT‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGCTGACCGGACCCATGAAAGGGGCGTGCGTAGGCGCATGTCTGTTCGAAGGTTGGGTGAAAGATGAAGCGCAGTCTCTGGCAATACTGGAGTA‑GGGCGAAGTGATCATCATTCCTTGTCTCAAtgtg                                                                           <  2:1117637/246‑1 (MQ=255)
                                                                                                                                                                            gAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATg                                                                       >  2:827196/1‑250 (MQ=255)
                                                                                                                                                                                aaaTTCGTCGCGCTCGTCCACGCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc                                                                                                                                                                                               <  2:91062/127‑1 (MQ=255)
                                                                                                                                                                                aaaTTCGTCGCGCTCGTCCACGCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc                                                                                                                                                                                               >  1:91062/1‑127 (MQ=255)
                                                                                                                                                                                 aaTTCGTCGCGCTCGTCCACACTTGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCTAACTAAACGAGGGC‑AGAACACTGCATCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGGAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                                                  <  2:66184/250‑1 (MQ=255)
                                                                                                                                                                                     cgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCCTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                                                              >  1:49753/1‑250 (MQ=255)
                                                                                                                                                                                           gcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTg                                                                                                   <  2:1157670/207‑1 (MQ=255)
                                                                                                                                                                                           gcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTg                                                                                                   >  1:1157670/1‑207 (MQ=255)
                                                                                                                                                                                                tCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                                                   <  1:827196/250‑1 (MQ=255)
                                                                                                                                                                                                  cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAgg                                                                                                                                 <  1:703279/171‑1 (MQ=255)
                                                                                                                                                                                                      ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt                                             <  2:321417/250‑1 (MQ=255)
                                                                                                                                                                                                       tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAGCTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACtt                                            >  1:119813/1‑250 (MQ=255)
                                                                                                                                                                                                       tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCa                                                                                  >  1:724041/1‑212 (MQ=255)
                                                                                                                                                                                                       tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCa                                                                                  <  2:724041/212‑1 (MQ=255)
                                                                                                                                                                                                       tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACtt                                            <  1:1334869/250‑1 (MQ=255)
                                                                                                                                                                                                        ggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg                                                                    >  1:1036358/1‑225 (MQ=255)
                                                                                                                                                                                                        ggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCAGTCCTTGTCACCATGTGAATGCCg                                                                    <  2:1036358/225‑1 (MQ=255)
                                                                                                                                                                                                         gctgaATGTGCAACCGGCCTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCt                                          <  2:49753/250‑1 (MQ=255)
                                                                                                                                                                                                              aTGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg                                     >  1:565655/1‑250 (MQ=255)
                                                                                                                                                                                                                      ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt                                                                   >  2:337147/1‑212 (MQ=255)
                                                                                                                                                                                                                          cttcttCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATg                                 >  2:683398/1‑242 (MQ=255)
                                                                                                                                                                                                                          cttcttCACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGAAGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATg                                 <  1:683398/242‑1 (MQ=255)
                                                                                                                                                                                                                           ttcttcACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCAc                                                                                 >  2:656952/1‑193 (MQ=255)
                                                                                                                                                                                                                           ttcttcACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCAc                                                                                 <  1:656952/193‑1 (MQ=255)
                                                                                                                                                                                                                             cttcACTTATCAGCGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAAt                                                                                                                       >  1:1003053/1‑154 (MQ=255)
                                                                                                                                                                                                                                          ccaaCTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAACGCGCTGGCAGGAGATGACCGGACCCATGCAAGGGGCGTGCGTGCGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCGGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGnagtgn         >  2:799158/3‑246 (MQ=255)
                                                                                                                                                                                                                                          cGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTc                                                                                   <  1:95702/176‑1 (MQ=255)
                                                                                                                                                                                                                                          cGAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTc                                                                                   >  2:95702/1‑176 (MQ=255)
                                                                                                                                                                                                                                           gAACTAAACGAGGGC‑AAAACACTGCTTCACGCCTGGCCGCCAATGCGCTGGCAGGAGATGACCGGATCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGTTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAGGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGCGGTTGAGAActgga        >  2:1224640/1‑246 (MQ=255)
                                                                                                                                                                                                                                           gAACTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGa        >  1:686340/1‑250 (MQ=255)
                                                                                                                                                                                                                                            aaCTAAACGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCATCGAGCCAATGGGCGGTATTCCTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGAc       <  1:1224640/250‑1 (MQ=255)
                                                                                                                                                                                                                                              cTAAACGAGGGT‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCTGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCg     >  2:1300525/1‑250 (MQ=255)
                                                                                                                                                                                                                                                aaaCGAGGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAGTGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGAc   <  2:1059973/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 aaCGAGGGN‑AAAACACTGGGTAGCGCGGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCAGGAAAGGGGCGTGCGTGGGCGCGTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCGCCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACg  <  2:686340/250‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
ATCGGATTACGCAGCTTTGCGCTGGAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTC‑CCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGC‑AAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACG  >  NC_007779_cfb/547449‑547937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A