Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F999 I1 R1 225 156.4 11709729 83.8% 9812752 75.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,957,124 +G intergenic (+58/+26) ilvC → / ← ppiC ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,957,1231.G95.1% 226.6 / 7.3 103intergenic (+57/+27)ilvC/ppiCketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand):  ref base . (5/0);  new base G (44/53);  total (50/53)
Fisher's exact test for biased strand distribution p-value = 2.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑A‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGT  >  NC_000913/3957050‑3957194
                                                                                |                                                                        
ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑a‑a                                                                           >  2:2183171/1‑76 (MQ=17)
ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑a‑a                                                                           >  1:4098605/1‑76 (MQ=17)
  gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGTGGGAGAAAACACTC‑A‑A‑gg                                                                        >  1:667915/1‑75 (MQ=17)
  gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑A‑gg                                                                        >  2:3331788/1‑75 (MQ=17)
  gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑A‑gg                                                                        >  1:2672247/1‑75 (MQ=17)
   cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑A‑ggc                                                                       <  2:4829245/76‑3 (MQ=17)
   cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑A‑ggc                                                                       >  1:2894758/1‑74 (MQ=17)
    tGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑A‑ggcc                                                                      >  2:4053990/1‑73 (MQ=17)
    tGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑A‑ggcc                                                                      <  1:2029390/76‑4 (MQ=17)
    tGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑A‑ggcc                                                                      >  2:3383084/1‑73 (MQ=17)
    tGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑A‑ggcc                                                                      <  2:2672247/76‑4 (MQ=17)
       tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTtc                                                                   <  2:5180557/76‑1 (MQ=14)
       tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTtc                                                                   <  2:773407/76‑1 (MQ=14)
        gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTtct                                                                  >  1:3556923/1‑76 (MQ=14)
        gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTtct                                                                  <  1:3918899/76‑1 (MQ=14)
        gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTtct                                                                  <  1:1389653/76‑1 (MQ=14)
         cGGGTTAAGTGCGCGCTGATGGCCCCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGGAAACACACAA‑A‑G‑CCGtctc                                                                 >  1:3648481/1‑76 (MQ=2)
          gggTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCCCCGGGGGCGGGAGGAAACACTCAA‑A‑G‑CCCTCTcc                                                                >  1:2448633/1‑76 (MQ=9)
          gggTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTcc                                                                >  1:3834939/1‑76 (MQ=14)
           ggTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCt                                                               <  1:494626/76‑1 (MQ=14)
           ggTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCt                                                               <  2:5838529/76‑1 (MQ=14)
              tAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTgta                                                            <  1:2826526/76‑3 (MQ=14)
              tAAGGGCGCGCTGATGCCCTCACCCCC‑‑ACCCTCTCCCACAGGGAGAGGGGGACAACACTC‑ACA‑G‑CGTTCTCCTggc                                                            >  2:63938/1‑75 (MQ=2)
               aattGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGgag                                                           <  2:2108225/73‑1 (MQ=14)
               aaGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGaag                                                           >  1:5059748/1‑76 (MQ=14)
                aGTGCGCGCTGATGCCCTCACCCCG‑‑ACGCTGTCGCGCGGGGGGAGGGAGAAAACACTC‑A‑GGG‑CCTTCTCCTGgaga                                                          <  2:453276/76‑1 (MQ=9)
                aGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGgaga                                                          <  2:184316/76‑1 (MQ=14)
                 gTGCGCGCTGCTGCCCTCACCCCG‑‑ACCCGCTCCCGCAGTGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGaa                                                         <  2:1926414/76‑1 (MQ=9)
                 gTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGaa                                                         <  1:740381/76‑1 (MQ=14)
                 gTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGaa                                                         <  2:5297093/76‑1 (MQ=14)
                 gTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGaa                                                         <  2:5185756/76‑1 (MQ=14)
                  tGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGgagacg                                                        >  2:1598999/1‑74 (MQ=14)
                  tGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAg                                                        <  2:4408198/76‑1 (MQ=17)
                  tGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAg                                                        <  2:2604769/76‑1 (MQ=14)
                   gcgcgcTGATGCCCTCACCCGT‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAgg                                                       <  1:2184182/76‑1 (MQ=17)
                   gcgcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAgg                                                       >  2:3241600/1‑76 (MQ=17)
                    cgcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGc                                                      <  1:5745061/76‑1 (MQ=17)
                     gcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGcc                                                     >  1:1500671/1‑76 (MQ=17)
                     gcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGcc                                                     >  1:4704333/1‑76 (MQ=17)
                     gcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGcc                                                     >  1:419565/1‑76 (MQ=17)
                     gcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGcc                                                     >  1:3271886/1‑76 (MQ=17)
                     gcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCCTCTCCTGGAGAAGGcc                                                     >  1:3257383/1‑76 (MQ=17)
                      cgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCt                                                    <  2:4455165/76‑1 (MQ=17)
                      cgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCt                                                    >  2:4567663/1‑76 (MQ=17)
                       gcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCCTCTCCTGGCGAAGGccct                                                   >  2:2552597/1‑74 (MQ=14)
                       gcTGATGCCCTCACCCAG‑‑ACCCTCTCCCACACGGAGAGGGAGAAAACACAC‑A‑AGG‑CCTTCTCCGCCAGAAGGCCta                                                   >  2:2485822/1‑75 (MQ=2)
                        cTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTg                                                  <  2:5258299/76‑1 (MQ=18)
                        cTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTg                                                  <  2:4338484/76‑1 (MQ=18)
                         tGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGc                                                 <  2:4587282/76‑1 (MQ=18)
                          gATGCCCTCACCCCGGCG‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑CGG‑CCTTCTCCTGGAGAAGGCCTTGCt                                                <  2:5579256/76‑1 (MQ=17)
                          gATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCt                                                >  2:4392837/1‑76 (MQ=18)
                          gATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCt                                                >  2:4500325/1‑76 (MQ=18)
                          gATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCt                                                <  1:3334456/76‑1 (MQ=18)
                           aTGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCtn                                               <  1:2937085/76‑2 (MQ=18)
                              cccTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGCAGGCCTTGCTATTa                                            >  2:5566711/1‑76 (MQ=18)
                              cccTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTa                                            >  1:753237/1‑76 (MQ=21)
                              cccTCACCCCG‑‑ACCCTCGCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTa                                            <  2:5179180/76‑1 (MQ=21)
                                cTCACCCCG‑‑ACCCTCTCCCACAGGGAGCGGGAGAAAACACTC‑A‑ACG‑CCTTCTCCTGGAGCAGGCCTTGCTACTAGt                                          >  2:2920327/1‑76 (MQ=18)
                                cTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGt                                          >  1:4638063/1‑76 (MQ=21)
                                cTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGt                                          <  1:1019727/76‑1 (MQ=21)
                                 tCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑ACG‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGtt                                         >  1:4734886/1‑76 (MQ=21)
                                 tCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGtt                                         <  2:3556923/76‑1 (MQ=21)
                                           accctctCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                 <  1:3976284/73‑1 (MQ=38)
                                           accctctCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                 >  2:2578911/4‑76 (MQ=38)
                                            ccctctCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAg                                <  1:2183171/74‑1 (MQ=38)
                                             cctctCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                               >  1:1417336/2‑76 (MQ=255)
                                             cctctCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                               <  2:2360700/75‑1 (MQ=255)
                                             cctctCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                               >  2:4641426/2‑76 (MQ=255)
                                             cctctCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                               <  1:2440930/75‑1 (MQ=255)
                                             cctctCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                               <  1:2747566/75‑1 (MQ=255)
                                             cctctCCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                               <  1:3971592/75‑1 (MQ=255)
                                               tctcCCGCAGGGAGAGGGAGAAACCACGC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTATTTGCGGTACAGCAc                             <  1:181878/76‑1 (MQ=255)
                                                ctcCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAcc                            <  1:4641427/76‑1 (MQ=255)
                                                 tcCCACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt                           <  2:1074753/76‑1 (MQ=255)
                                                  cccACAGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCtt                          >  2:4331914/1‑76 (MQ=255)
                                                     acaGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                       >  1:682085/1‑76 (MQ=255)
                                                     acaGGGAGAGGGAGAAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                       <  1:2920327/76‑1 (MQ=255)
                                                         ggagagggagaAAACACGC‑A‑AGG‑CCTTATCCTGGAGAAGGCCTTGCTATTAGTTGCGGGACAGCACCTTAatgatg                   >  1:1673822/1‑76 (MQ=255)
                                                          gagagggagaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAGTGAtgt                  <  2:419565/76‑1 (MQ=255)
                                                            gagggagaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGGTGCGGTACAGCACCTTAATGATGTGa                >  1:4010810/1‑76 (MQ=255)
                                                             agggagaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat               >  2:3615936/1‑76 (MQ=255)
                                                              gggagaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata              <  1:1466827/76‑1 (MQ=255)
                                                              gggagaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTAGTAGTTGCGGTACAGCACCTTAATGATGAGata              >  2:1775617/1‑76 (MQ=255)
                                                               ggagaAAACACTC‑A‑AGG‑CCTACTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGACGTGatac             >  2:563763/1‑75 (MQ=255)
                                                                gagaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTCTTAGGTGCGGTACAGCATCTTCATGCTGTGATATc            >  1:2860064/1‑76 (MQ=255)
                                                                gagaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTACTGATGTGATATc            >  2:4638546/1‑76 (MQ=255)
                                                                gagaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc            <  2:3023906/76‑1 (MQ=255)
                                                                gagaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc            <  1:360313/76‑1 (MQ=255)
                                                                 agaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc           <  1:5812144/76‑1 (MQ=255)
                                                                  gaAAACACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCg          >  2:3104970/1‑76 (MQ=255)
                                                                   aaaaCACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa         >  1:4421794/1‑76 (MQ=255)
                                                                   aaaaCACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa         >  2:5297681/1‑76 (MQ=255)
                                                                    aaaCACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAGATCAGaa        >  1:1915995/1‑76 (MQ=255)
                                                                     aaCACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAc       <  1:1071117/76‑1 (MQ=255)
                                                                     aaCACTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAc       <  1:3139331/76‑1 (MQ=255)
                                                                       cacTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCCCCTTAATGATGTGATATCCGCACTg     >  2:333848/1‑76 (MQ=255)
                                                                       cacTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg     <  1:1598999/76‑1 (MQ=255)
                                                                        acTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg    >  1:896231/1‑76 (MQ=255)
                                                                         cTC‑A‑AGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg   >  2:5244521/1‑76 (MQ=255)
                                                                          tC‑A‑A‑GCCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGACATCCGCACTgcgg  >  2:784551/1‑73 (MQ=255)
                                                                                |                                                                        
TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑A‑A‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGT  >  NC_000913/3957050‑3957194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A