Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F999 I1 R1
|
226 |
132.7 |
9745491 |
85.7% |
8351885 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,558,932 |
2 bp→GC |
coding (153‑154/831 nt) |
glpG ← |
GlpG |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,558,932 | 0 | C | G | 100.0%
| 268.5
/ NA
| 103 | V52L (GTG→CTG) | glpG | GlpG |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (57/46); total (57/46) |
| * | NC_000913 | 3,558,933 | 0 | G | C | 100.0%
| 277.5
/ NA
| 103 | D51E (GAC→GAG) | glpG | GlpG |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (57/46); total (57/46) |
Only 100 of 104 total aligned reads displayed.
GTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCACGTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGG > NC_000913/3558865‑3559008
||
gTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGccc > 1:2342002/1‑76 (MQ=255)
gTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGccc > 1:3609422/1‑76 (MQ=255)
gTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGccc < 1:1954097/76‑1 (MQ=255)
tGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGtc > 2:3402388/1‑75 (MQ=255)
tGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTa > 2:732137/1‑76 (MQ=255)
tGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTa < 1:4453091/76‑1 (MQ=255)
tGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTa > 2:3900536/1‑76 (MQ=255)
ggCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAc > 1:2712522/1‑76 (MQ=255)
ggCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAc < 1:4439929/76‑1 (MQ=255)
gcctgcctGCCAGCTCGCCGCCAGATACCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcg > 2:3616903/1‑76 (MQ=255)
gcctgcctGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcg < 1:2694810/76‑1 (MQ=255)
cctgcctgCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcgc < 1:1608283/76‑1 (MQ=255)
cctgcctgCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcgc > 1:2699133/1‑76 (MQ=255)
tgcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACgcgc > 1:4513822/1‑76 (MQ=255)
tgcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACgcgc > 1:468722/1‑76 (MQ=255)
tgcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGCACgcgc > 2:2436674/1‑76 (MQ=255)
ccctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCt < 1:3417235/75‑1 (MQ=255)
cctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTc < 1:3173361/76‑1 (MQ=255)
ctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCg > 1:4767151/1‑76 (MQ=255)
ctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCCCg > 2:2658558/1‑76 (MQ=255)
tgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGCACGCGCTCgc > 2:1629023/1‑75 (MQ=255)
cgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCgg < 1:1014976/75‑1 (MQ=255)
gccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGc < 1:2561992/76‑1 (MQ=255)
ccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGcc < 1:3558412/76‑1 (MQ=255)
ccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGcc > 2:3619147/1‑76 (MQ=255)
cAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCt < 1:4693728/76‑1 (MQ=255)
cAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCt < 1:2629551/76‑1 (MQ=255)
cAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCt > 2:4677667/1‑76 (MQ=255)
cAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGCACGCGCTCGGCCt > 2:1346556/1‑76 (MQ=255)
cTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTggg < 1:3286220/76‑1 (MQ=255)
cTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTggg > 2:2865078/1‑76 (MQ=255)
tCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCCCTCGGCCTGGGa > 2:283708/1‑76 (MQ=255)
tCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCCGCCTggcc > 2:1099072/1‑74 (MQ=255)
tCGCCGCCAGATAACGCGGATCTGCCGGGTCTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTgggc > 2:2262553/1‑75 (MQ=255)
ccgccgcCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCGCGTACGCGCTCGGCCTGGGa < 2:4719285/75‑1 (MQ=255)
cgccgcCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGAc < 1:3795805/76‑1 (MQ=255)
gccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACt < 2:1223258/76‑1 (MQ=255)
cccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACt < 2:2173834/75‑1 (MQ=255)
ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTc < 2:3017998/76‑1 (MQ=255)
ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTc < 2:3752698/76‑1 (MQ=255)
ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTc > 1:4227103/1‑76 (MQ=255)
cgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCa > 2:1559590/1‑76 (MQ=255)
cgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCa > 2:3526615/1‑76 (MQ=255)
gccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCac > 2:2582195/1‑75 (MQ=255)
gccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCAt > 2:3892722/1‑76 (MQ=255)
ccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATc > 1:1254416/1‑76 (MQ=255)
ccAGAGAGCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCTCCCGTACGCGCTCGGCCTGGGACTCATc < 2:4610392/76‑1 (MQ=255)
aGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCg < 2:3925871/76‑1 (MQ=255)
aGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCg < 1:4080594/76‑1 (MQ=255)
aTAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgcc > 1:1566485/1‑76 (MQ=255)
tAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgcca < 1:4707494/76‑1 (MQ=255)
aaCGCGGATCTGCCGGGTTTTCGAGAAACCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccag < 1:582116/76‑1 (MQ=255)
aaCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccag > 1:4140489/1‑76 (MQ=255)
aCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagc < 1:2716169/76‑1 (MQ=255)
aCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagc > 1:985937/1‑76 (MQ=255)
cgcgGATCTGCCGGGTTTTCGAGAAGACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagcc < 1:455033/76‑1 (MQ=255)
cgcgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagcc < 2:4109422/76‑1 (MQ=255)
cgcgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagcc < 1:1609525/76‑1 (MQ=255)
gcgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCTGCCTGGGACTCCTCCgcccgcca > 2:2011139/1‑76 (MQ=255)
cgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGccagccag > 1:2355348/1‑76 (MQ=255)
gggtCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa < 1:607913/73‑1 (MQ=255)
ggATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa < 2:4320097/76‑1 (MQ=255)
aTCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGCACTCATCCGCCAGCCAGACa > 1:1301201/1‑76 (MQ=255)
tCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCCGCCAGACac > 2:2096816/1‑75 (MQ=255)
tCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAt < 2:2355347/76‑1 (MQ=255)
gCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGc < 2:3814102/76‑1 (MQ=255)
ccGGGGTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCt > 1:2993803/1‑76 (MQ=255)
cGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCtt > 2:4384940/1‑76 (MQ=255)
cGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCtt > 1:3783816/1‑76 (MQ=255)
gggTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCttt > 2:526076/1‑76 (MQ=255)
gggTGTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCttt < 2:2044255/76‑1 (MQ=255)
cggTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCttt < 1:363466/75‑1 (MQ=255)
ggTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTg > 1:12694/1‑76 (MQ=255)
ggTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTg > 2:1274829/1‑76 (MQ=255)
gTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTgg > 1:4382607/1‑76 (MQ=255)
ttttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGt < 1:4648125/76‑1 (MQ=255)
ttttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGt > 1:1862033/1‑76 (MQ=255)
ttttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGt < 2:4351307/76‑1 (MQ=255)
tttCGAGAAACCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGtt < 1:2865078/76‑1 (MQ=255)
tttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGtt > 1:1480554/1‑76 (MQ=255)
tttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGtt < 1:2968527/76‑1 (MQ=255)
tgcGAGCAAACGCGCCAGCTCCGCCCGTACGCTCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTa < 2:2231022/74‑1 (MQ=255)
tCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAt < 1:3900536/76‑1 (MQ=255)
cGAGAAAACGCGCCAGCTCCGCCCGTCCGCGCCCGCCCTGGCACTCACCCCCCACCCCCACACCGCTTTGGTCAtt > 2:357097/1‑75 (MQ=255)
cGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtg > 2:2872056/1‑76 (MQ=255)
cGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtg < 1:4079354/76‑1 (MQ=255)
agaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTTGTTAtgtt > 1:2731628/1‑76 (MQ=255)
agaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgtt < 1:3663768/76‑1 (MQ=255)
agaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgtt < 2:4355276/76‑1 (MQ=255)
agaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgtt > 1:2140781/1‑76 (MQ=255)
gaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCCGCCAGACATCCCTTTGGTTAtgttg > 2:6532/1‑76 (MQ=255)
aaaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgttgt > 2:2576068/1‑76 (MQ=255)
aaaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGGTAtgttgt > 1:2685305/1‑76 (MQ=255)
aaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATgttgtt > 2:472184/1‑76 (MQ=255)
aaaCGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATgttgtt < 2:2173491/76‑1 (MQ=255)
aaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGttgttg > 1:2525096/1‑76 (MQ=255)
aaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGttgttg > 2:3459944/1‑76 (MQ=255)
aaCGCGCCAGCTCCCCCAGTACGCGCTCGGCCTGGGCCCCATCCTCCAGCCTGACCTAGATTTGGCTATgttgttc > 1:3168154/1‑75 (MQ=255)
aCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGa > 2:633919/1‑76 (MQ=255)
gTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAgg < 2:3932203/76‑1 (MQ=255)
||
GTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCACGTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGG > NC_000913/3558865‑3559008
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
