Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	461,242	G→A	785 (TAG→TAA)	lon →	DNA‑binding ATP‑dependent protease La

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	461,242	0	G	A	100.0%	136.6 / NA	43	785 (TAG→TAA)	lon	DNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/17); total (26/17)

GAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGATGG  >  NC_000913/461120‑461373

gAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCATCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAAt                                                                                                                     >  1:51698/1‑139 (MQ=255)

      aTTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACta                                                                                                               >  1:202413/1‑139 (MQ=255)

      aTTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACta                                                                                                               >  2:38400/1‑139 (MQ=255)

      aTTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACta                                                                                                               >  2:33431/1‑139 (MQ=255)

            gACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATaaaa                                                                                                         >  2:150948/1‑139 (MQ=255)

                 cGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAgg                                                                                                    >  1:353171/1‑139 (MQ=255)

                 cGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAgg                                                                                                    >  1:90068/1‑139 (MQ=255)

                 cGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAgg                                                                                                    >  1:668936/1‑139 (MQ=255)

                                  aCATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCg                                                                                   <  2:353171/139‑1 (MQ=255)

                                       cATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGCCAGGCTAATTCGGGCtt                                                                              >  1:538638/1‑139 (MQ=255)

                                         tCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGc                                                                            <  2:90068/139‑1 (MQ=255)

                                           cTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCa                                                                          >  2:20761/1‑139 (MQ=255)

                                              tgAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGcc                                                                       >  2:676627/1‑139 (MQ=255)

                                                  gcgcATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCa                                                                                                                  >  2:759067/1‑92 (MQ=255)

                                                  gcgcATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCa                                                                                                                  <  1:759067/92‑1 (MQ=255)

                                                  gcgcATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCtttt                                                                   >  1:763025/1‑139 (MQ=255)

                                                    gcATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCtttttt                                                                 >  2:135062/1‑139 (MQ=255)

                                                     cATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCttttttt                                                                >  1:397813/1‑139 (MQ=255)

                                                       ttGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGAGCTTGCCAGCCTTTTTTTGt                                                              >  1:764547/1‑139 (MQ=255)

                                                                ttCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTc                                                                                                                              <  1:88740/66‑1 (MQ=255)

                                                                ttCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTc                                                                                                                              >  2:88740/1‑66 (MQ=255)

                                                                ttCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTCGTCTCGCTAAg                                                     >  2:518891/1‑139 (MQ=255)

                                                                          ggCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCg                                           >  1:164008/1‑139 (MQ=255)

                                                                             gcTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGAt                                        <  2:681646/139‑1 (MQ=255)

                                                                                 cAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCggg                                    <  2:668936/139‑1 (MQ=255)

                                                                                   aaaTGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCt                                  <  1:38400/139‑1 (MQ=255)

                                                                                      tGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGc                               <  1:578499/139‑1 (MQ=255)

                                                                                       gAACCGTCCGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGcc                              <  1:486326/139‑1 (MQ=255)

                                                                                        aaCCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGccc                             <  2:51698/139‑1 (MQ=255)

                                                                                              cTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCttatta                       <  2:763025/139‑1 (MQ=255)

                                                                                               tGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTaa                      <  1:33431/139‑1 (MQ=255)

                                                                                                  tATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAggg                   <  1:676627/139‑1 (MQ=255)

                                                                                                  tATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAggg                   <  2:164008/139‑1 (MQ=255)

                                                                                                         gTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCt                                  >  1:604428/1‑117 (MQ=255)

                                                                                                         gTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCt                                  <  2:604428/117‑1 (MQ=255)

                                                                                                         gTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGtgttgt            >  1:591281/1‑139 (MQ=255)

                                                                                                           tgtgACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTaa          >  1:522579/1‑139 (MQ=255)

                                                                                                           tgtgACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTaa          >  1:537862/1‑139 (MQ=255)

                                                                                                              gACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCtt                           <  2:459803/119‑1 (MQ=255)

                                                                                                              gACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCtt                           >  1:459803/1‑119 (MQ=255)

                                                                                                                cTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGccc                             >  1:326327/1‑115 (MQ=255)

                                                                                                                cTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGccc                             <  2:326327/115‑1 (MQ=255)

                                                                                                                   cAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGAtgg  >  2:252530/1‑139 (MQ=255)

GAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGATGG  >  NC_000913/461120‑461373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A