Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,038	G→A	A325T (GCT→ACT)	dhaR →	dhaKLM operon transcription activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,038	0	G	A	100.0%	124.8 / NA	39	A325T (GCT→ACT)	dhaR	dhaKLM operon transcription activator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/18); total (21/18)

TTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATA  >  NC_000913/1251906‑1252173

tttATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                                   <  2:319339/139‑1 (MQ=255)

       aTGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCcaca                                                                                                                            >  2:345095/1‑139 (MQ=255)

          cGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGa                                                                                                                         <  1:479460/139‑1 (MQ=255)

          cGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGa                                                                                                                         >  1:517258/1‑139 (MQ=255)

            gTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACg                                                                                                                       >  2:775688/1‑139 (MQ=255)

             tGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGa                                                                                                                      <  2:491460/139‑1 (MQ=255)

              gATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGAt                                                                                                                     >  2:750156/1‑139 (MQ=255)

                       tAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAAcc                                                                                                            >  2:50556/1‑139 (MQ=255)

                              ataataGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCt                                                                                                     >  1:726173/1‑139 (MQ=255)

                                             ggAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTc                                                                                      >  1:268305/1‑139 (MQ=255)

                                                               ttgCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTa                                                                    >  2:530166/1‑139 (MQ=255)

                                                                     cATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtc                                                              <  2:288874/139‑1 (MQ=255)

                                                                          tgtgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtc                                                         <  2:517258/139‑1 (MQ=255)

                                                                            tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcct                                                       >  1:679802/1‑139 (MQ=255)

                                                                            tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcct                                                       >  2:619861/1‑139 (MQ=255)

                                                                             ggAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcctg                                                      >  2:403523/1‑139 (MQ=255)

                                                                             ggAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcctg                                                      <  2:145721/139‑1 (MQ=255)

                                                                                 cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCttt                                                  >  2:103408/1‑139 (MQ=255)

                                                                                   gATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTtgt                                                <  1:403523/139‑1 (MQ=255)

                                                                                    aTGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTtgtg                                               >  2:768467/1‑139 (MQ=255)

                                                                                     tGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTgg                                              <  2:679802/139‑1 (MQ=255)

                                                                                      gCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                                   <  2:77691/53‑1 (MQ=255)

                                                                                      gCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                                   >  1:77691/1‑53 (MQ=255)

                                                                                      gCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGga                                             >  1:267162/1‑139 (MQ=255)

                                                                                         gCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCttt                                                  <  2:355885/131‑1 (MQ=255)

                                                                                         gCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCttt                                                  >  1:355885/1‑131 (MQ=255)

                                                                                              tgatgaCCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtc                                                         <  2:525240/119‑1 (MQ=255)

                                                                                              tgatgaCCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtc                                                         >  1:525240/1‑119 (MQ=255)

                                                                                                      aGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCa                             >  1:496615/1‑139 (MQ=255)

                                                                                                       gTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtc                                                              >  1:681031/1‑105 (MQ=255)

                                                                                                       gTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtc                                                              <  2:681031/105‑1 (MQ=255)

                                                                                                          aaTTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGc                         <  1:619861/139‑1 (MQ=255)

                                                                                                           aTTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCa                        <  1:345095/139‑1 (MQ=255)

                                                                                                                  aaaGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTaa                 >  1:690338/1‑139 (MQ=255)

                                                                                                                        aGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAgg           <  1:768467/139‑1 (MQ=255)

                                                                                                                         gCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGc          <  1:750156/139‑1 (MQ=255)

                                                                                                                          ccATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGGAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGc                         >  1:489972/1‑123 (MQ=255)

                                                                                                                          ccATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCCTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGc                         <  2:489972/123‑1 (MQ=255)

                                                                                                                                 ttCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATa  <  1:103408/139‑1 (MQ=255)

TTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATA  >  NC_000913/1251906‑1252173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A