Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,353,081	G→A	58 (TAG→TAA)	ghoT →	toxin of GhoTS toxin‑antitoxin pair, membrane‑lytic protein, stimulator of persister cell formation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,353,081	0	G	A	100.0%	97.0 / NA	31	58 (TAG→TAA)	ghoT	toxin of GhoTS toxin‑antitoxin pair, membrane‑lytic protein, stimulator of persister cell formation
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/10); total (21/10)

TCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGC  >  NC_000913/4352959‑4353213

tCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACCTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGt                                                                                                                       >  2:699792/1‑139 (MQ=255)

tCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTg                                                                                                                      >  1:483722/1‑139 (MQ=255)

tCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTACCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTg                                                                                                                      >  1:580743/1‑139 (MQ=255)

           tATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGt                                                                                                           >  1:401744/1‑139 (MQ=255)

            aTCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGtt                                                                                                          >  1:38731/1‑139 (MQ=255)

                 gTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGt                                                                                                     >  2:776779/1‑139 (MQ=255)

                 gTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGt                                                                                                     >  1:261108/1‑139 (MQ=255)

                     ctctcACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGaaa                                                                                                 >  1:499762/2‑139 (MQ=255)

                               agaAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGtct                                                                                       >  1:35752/1‑139 (MQ=255)

                                                    ttAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCg                                                                  <  1:96514/139‑1 (MQ=255)

                                                      aGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGa                                                                <  2:401744/139‑1 (MQ=255)

                                                        tGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGc                                                                                                               >  1:513843/1‑90 (MQ=255)

                                                        tGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGc                                                                                                               <  2:513843/90‑1 (MQ=255)

                                                        tGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAAc                                                              >  1:439959/1‑139 (MQ=255)

                                                        tGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAAc                                                              >  2:144325/1‑139 (MQ=255)

                                                             tCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGa                                                         >  1:130813/1‑139 (MQ=255)

                                                                  gTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGtct                                                                                       >  2:13391/1‑104 (MQ=255)

                                                                  gTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGtct                                                                                       <  1:13391/104‑1 (MQ=255)

                                                                    cGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGc                                                  <  1:776779/139‑1 (MQ=255)

                                                                            ccTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCt                                          <  2:261108/139‑1 (MQ=255)

                                                                              tGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGa                                        <  1:144325/139‑1 (MQ=255)

                                                                                 ccAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTg                                     >  2:20394/1‑139 (MQ=255)

                                                                                 ccAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTg                                     >  2:215529/1‑139 (MQ=255)

                                                                                 ccAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTg                                     >  2:9068/1‑139 (MQ=255)

                                                                                 ccAATGAGTCTACCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTg                                     <  2:580743/139‑1 (MQ=255)

                                                                                          cTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCa                            <  1:688539/139‑1 (MQ=255)

                                                                                                  ggCATTA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTg                    >  2:190551/1‑139 (MQ=255)

                                                                                                       tA‑CTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAatt               >  1:780913/1‑139 (MQ=255)

                                                                                                                     ctTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGgc  >  1:590023/1‑139 (MQ=255)

                                                                                                                       tttAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGt                                                                                         <  1:607313/50‑1 (MQ=255)

                                                                                                                       tttAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGt                                                                                         >  2:607313/1‑50 (MQ=255)

TCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTA‑CTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGC  >  NC_000913/4352959‑4353213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A