Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
777 |
59.2 |
2955938 |
92.5% |
2734242 |
106.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,082,943 |
A→G |
Q234R (CAG→CGG) |
efeO → |
inactive ferrous ion transporter EfeUOB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,082,943 | 0 | A | G | 100.0%
| 142.4
/ NA
| 40 | Q234R (CAG→CGG) | efeO | inactive ferrous ion transporter EfeUOB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/23); total (17/23) |
CTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTG > NC_000913/1082812‑1083077
|
ctcCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCt > 2:564956/1‑139 (MQ=255)
cGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGag < 1:1013166/139‑1 (MQ=255)
cGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGag < 1:771119/139‑1 (MQ=255)
cGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGag < 1:564956/139‑1 (MQ=255)
cGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGag < 2:742066/139‑1 (MQ=255)
cGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGag < 2:391211/139‑1 (MQ=255)
aTCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGagca > 2:5169/1‑139 (MQ=255)
tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCt > 2:246212/1‑139 (MQ=255)
gATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCt > 1:268452/1‑137 (MQ=255)
gATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCt < 2:268452/137‑1 (MQ=255)
aaaaGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGaa > 2:1322046/1‑139 (MQ=255)
aaaGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAAc < 2:1210468/139‑1 (MQ=255)
cgccgACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCt < 1:316427/139‑1 (MQ=255)
gTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCaaaaa < 1:137683/101‑1 (MQ=255)
gTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCaaaaa > 2:137683/1‑101 (MQ=255)
aCCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTcggcgg < 1:246212/139‑1 (MQ=255)
tCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTa < 2:480669/51‑1 (MQ=255)
tCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTa > 1:480669/1‑51 (MQ=255)
tCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCaa > 1:709202/1‑88 (MQ=255)
tCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCaa < 2:709202/88‑1 (MQ=255)
tGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGc > 1:661381/1‑139 (MQ=255)
aGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTg < 1:470502/139‑1 (MQ=255)
gCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAAc > 2:126883/1‑94 (MQ=255)
gCATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAAc < 1:126883/94‑1 (MQ=255)
cATTGTTTGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGAt < 2:555419/139‑1 (MQ=255)
tttGGCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAgg < 2:661381/139‑1 (MQ=255)
gCGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGt > 2:723870/1‑139 (MQ=255)
cGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGa < 2:1225598/124‑1 (MQ=255)
cGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGa > 1:1225598/1‑124 (MQ=255)
cGACAACACCACCAAAGGGATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTg > 2:981182/1‑139 (MQ=255)
gggATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCaaaa < 2:185079/53‑1 (MQ=255)
gggATGGATCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCaaaa > 1:185079/1‑53 (MQ=255)
ggatggatCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAg < 1:5169/139‑1 (MQ=255)
atggatCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCg < 2:637898/139‑1 (MQ=255)
ggatCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACg < 1:946064/51‑1 (MQ=255)
ggatCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACg > 2:946064/1‑51 (MQ=255)
ggatCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGt > 1:552618/1‑139 (MQ=255)
gatCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGa > 2:1015854/1‑102 (MQ=255)
gatCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGa < 1:1015854/102‑1 (MQ=255)
gatCGGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTg < 1:139488/139‑1 (MQ=255)
|
CTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTG > NC_000913/1082812‑1083077
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
