Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,125,011	A→G	C106R (TGC→CGC)	mdtH ←	multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,125,011	0	A	G	100.0%	110.5 / NA	32	C106R (TGC→CGC)	mdtH	multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/14); total (18/14)

TGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACC  >  NC_000913/1124879‑1125146

tGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGaaaa                                                                                                                                   >  1:758186/1‑139 (MQ=255)

                 tCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGtt                                                                                                                  >  2:18127/1‑139 (MQ=255)

                 tCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGtt                                                                                                                  >  2:881279/1‑139 (MQ=255)

                 tCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGtt                                                                                                                  >  1:129613/1‑139 (MQ=255)

                   cTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCg                                                                                                                <  2:268855/139‑1 (MQ=255)

                        tcatcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGc                                                                                                           <  1:881279/139‑1 (MQ=255)

                           tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGAt                                                                                                        >  2:975615/1‑139 (MQ=255)

                           tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGAt                                                                                                        >  1:619529/1‑139 (MQ=255)

                           tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGAt                                                                                                        >  1:990992/1‑139 (MQ=255)

                                                         gtgGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCAt                                                                          <  2:543803/139‑1 (MQ=255)

                                                                 aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTg                                                                                                                                       <  1:1262285/70‑1 (MQ=255)

                                                                 aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTg                                                                                                                                       >  2:1262285/1‑70 (MQ=255)

                                                                 aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                       >  1:1358639/1‑86 (MQ=255)

                                                                 aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                       <  2:1358639/86‑1 (MQ=255)

                                                                 aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATAc                                                                                                      >  2:108530/1‑103 (MQ=255)

                                                                 aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATAc                                                                                                      <  1:108530/103‑1 (MQ=255)

                                                                    aaTTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCgg                                                               >  2:668661/1‑139 (MQ=255)

                                                                       ttCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTaa                                                            <  2:990992/139‑1 (MQ=255)

                                                                          accaccAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACaa                                                         >  2:787590/1‑139 (MQ=255)

                                                                           ccaccaGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGGGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGCGGGCGATACCCAGTGTGGCGAATCCGCCGACGCGCATCAGCATACCGGTAACAat                                                        >  1:197808/1‑139 (MQ=255)

                                                                                                    aaaCAACGTGCCACTGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCg                               >  2:1439905/1‑139 (MQ=255)

                                                                                                           gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTg                        >  1:892802/1‑139 (MQ=255)

                                                                                                            tGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAg                                                                       <  1:665440/91‑1 (MQ=255)

                                                                                                            tGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAg                                                                       >  2:665440/1‑91 (MQ=255)

                                                                                                             gCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCa                      <  2:758186/139‑1 (MQ=255)

                                                                                                             gCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCa                      >  2:1105996/1‑139 (MQ=255)

                                                                                                             gCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCa                      <  1:417042/139‑1 (MQ=255)

                                                                                                               cACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCAcc                    >  1:395086/1‑139 (MQ=255)

                                                                                                                 ccGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCAccgc                  <  1:1105996/139‑1 (MQ=255)

                                                                                                                      tCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGaaa             <  2:1374107/139‑1 (MQ=255)

                                                                                                                               cAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGa    <  2:395086/139‑1 (MQ=255)

                                                                                                                                 ggCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGAcc  <  1:668661/139‑1 (MQ=255)

TGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACC  >  NC_000913/1124879‑1125146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A