Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I2 R1 777 59.2 2955938 92.5% 2734242 106.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,389,242 C→T Q105* (CAA→TAA)  ycjG → L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,389,2420CT100.0% 256.5 / NA 79Q105* (CAA→TAA) ycjGL‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/39);  total (40/39)

CGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGC  >  NC_000913/1389108‑1389361
                                                                                                                                      |                                                                                                                       
cgcAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATc                                                                                                                     >  2:1137639/1‑139 (MQ=255)
               tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                      >  1:55156/1‑139 (MQ=255)
               tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                      >  1:609356/1‑139 (MQ=255)
               tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                      >  1:1214729/1‑139 (MQ=255)
               tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                      <  2:791651/139‑1 (MQ=255)
                cgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATc                                                                                                     >  1:666333/1‑139 (MQ=255)
                         aCTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACg                                                                                            <  2:218958/139‑1 (MQ=255)
                                  aGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                   <  1:1429762/139‑1 (MQ=255)
                                     gCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACa                                                                                >  2:932266/1‑139 (MQ=255)
                                        gACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGtt                                                                             >  1:373235/1‑139 (MQ=255)
                                        gACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGtt                                                                             >  1:117935/1‑139 (MQ=255)
                                         acacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTa                                                                            >  1:1399950/1‑139 (MQ=255)
                                          cacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                           <  1:1137639/139‑1 (MQ=255)
                                            cGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCa                                                                         <  2:589510/139‑1 (MQ=255)
                                              ggaggaGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACt                                                                       >  2:472096/1‑139 (MQ=255)
                                                 ggagTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGca                                                                    <  1:932266/139‑1 (MQ=255)
                                                    gTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACg                                                                                            >  1:1407818/1‑112 (MQ=255)
                                                    gTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACg                                                                                            <  2:1407818/112‑1 (MQ=255)
                                                               ttCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGGTCGGCATAACGCTTCCCgag                                                                                   <  2:701381/110‑1 (MQ=255)
                                                               ttCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGGTCGGCATAACGCTTCCCgag                                                                                   >  1:701381/1‑110 (MQ=255)
                                                               ttCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCAt                                                      <  2:1399950/139‑1 (MQ=255)
                                                                     cTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAc                                                <  2:1214729/139‑1 (MQ=255)
                                                                          ggCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTg                                           <  2:373235/139‑1 (MQ=255)
                                                                               agcaCGTAATGCGCTGGTTTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                      <  1:1301630/139‑1 (MQ=255)
                                                                                gcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGa                                     <  2:2216/139‑1 (MQ=255)
                                                                                      aaTGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCa                               >  2:148737/1‑139 (MQ=255)
                                                                                        tGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAAt                             <  2:790363/139‑1 (MQ=255)
                                                                                        tGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAAt                             <  2:666333/139‑1 (MQ=255)
                                                                                           gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGt                          >  2:499917/1‑139 (MQ=255)
                                                                                           gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGt                          >  2:465026/1‑139 (MQ=255)
                                                                                            cTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACg                                                                                            >  1:598197/1‑72 (MQ=255)
                                                                                            cTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACg                                                                                            <  2:598197/72‑1 (MQ=255)
                                                                                              ggATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAc                                                <  1:1159023/114‑1 (MQ=255)
                                                                                              ggATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAc                                                >  2:1159023/1‑114 (MQ=255)
                                                                                                aTTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATc                     <  2:55156/139‑1 (MQ=255)
                                                                                                aTTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATc                     >  2:753272/1‑139 (MQ=255)
                                                                                                    tgCATTGTGGTATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGc                                              <  1:1368091/110‑1 (MQ=255)
                                                                                                    tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGc                                              >  2:1368091/1‑110 (MQ=255)
                                                                                                    tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                      >  1:86001/1‑118 (MQ=255)
                                                                                                    tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                      <  2:86001/118‑1 (MQ=255)
                                                                                                         tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgaga                                                                                  >  1:61320/1‑69 (MQ=255)
                                                                                                         tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgaga                                                                                  <  2:61320/69‑1 (MQ=255)
                                                                                                         tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                              >  2:650276/1‑73 (MQ=255)
                                                                                                         tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                              <  1:650276/73‑1 (MQ=255)
                                                                                                         tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                        <  1:199251/95‑1 (MQ=255)
                                                                                                         tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                        >  2:199251/1‑95 (MQ=255)
                                                                                                         tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                   >  1:471499/1‑100 (MQ=255)
                                                                                                         tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                   <  2:471499/100‑1 (MQ=255)
                                                                                                         tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATc                                        >  2:657760/1‑111 (MQ=255)
                                                                                                         tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATc                                        <  1:657760/111‑1 (MQ=255)
                                                                                                          gtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                      >  1:530664/1‑112 (MQ=255)
                                                                                                          gtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                      <  2:530664/112‑1 (MQ=255)
                                                                                                           tgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTa                                                 >  2:529528/1‑100 (MQ=255)
                                                                                                           tgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTa                                                 <  1:529528/100‑1 (MQ=255)
                                                                                                           tgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAc                                                <  2:585718/101‑1 (MQ=255)
                                                                                                           tgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATc                                        >  2:659082/1‑109 (MQ=255)
                                                                                                           tgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATc                                        <  1:659082/109‑1 (MQ=255)
                                                                                                           tgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTGGTCATCGGTAc                                                >  1:585718/1‑101 (MQ=255)
                                                                                                                tCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCAt                                                                                                <  2:980632/48‑1 (MQ=255)
                                                                                                                tCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCAt                                                                                                >  1:980632/1‑48 (MQ=255)
                                                                                                                tCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAc                                                >  2:1328120/1‑96 (MQ=255)
                                                                                                                tCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAc                                                <  1:1328120/96‑1 (MQ=255)
                                                                                                                   ggCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACg                                               <  2:966109/94‑1 (MQ=255)
                                                                                                                   ggCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACg                                               >  1:966109/1‑94 (MQ=255)
                                                                                                                   ggCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCaggcaggc  >  2:965078/1‑139 (MQ=255)
                                                                                                                    gCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGcac                                                                   <  1:772184/73‑1 (MQ=255)
                                                                                                                    gCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGcac                                                                   >  2:772184/1‑73 (MQ=255)
                                                                                                                     ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACg                                                                                            <  1:829869/47‑1 (MQ=255)
                                                                                                                     ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACg                                                                                            >  2:829869/1‑47 (MQ=255)
                                                                                                                     ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgaga                                                                                  >  2:1336315/1‑57 (MQ=255)
                                                                                                                     ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgaga                                                                                  <  1:1336315/57‑1 (MQ=255)
                                                                                                                     ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                   <  2:432647/88‑1 (MQ=255)
                                                                                                                     ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                   >  1:432647/1‑88 (MQ=255)
                                                                                                                     ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTa                                                 >  2:154217/1‑90 (MQ=255)
                                                                                                                     ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTa                                                 <  1:154217/90‑1 (MQ=255)
                                                                                                                     ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATc                                        >  1:150808/1‑99 (MQ=255)
                                                                                                                     ccGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATc                                        <  2:150808/99‑1 (MQ=255)
                                                                                                                       gcgcgAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTa                                                 <  1:981725/88‑1 (MQ=255)
                                                                                                                       gcgcgAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTa                                                 >  2:981725/1‑88 (MQ=255)
                                                                                                                                      |                                                                                                                       
CGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGC  >  NC_000913/1389108‑1389361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A