Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,344	G→A	A38V (GCT→GTT)	ydaG ←	Rac prophage, uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,344	0	G	A	100.0%	205.7 / NA	64	A38V (GCT→GTT)	ydaG	Rac prophage, uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/30); total (34/30)

TTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCG  >  NC_000913/1419213‑1419478

tttGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTc                                                                                                                                 >  1:891763/1‑139 (MQ=255)

    cTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTctgcc                                                                                                                             <  2:891763/139‑1 (MQ=255)

           tataCGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTctgcctg                                                                                                                           >  2:721929/1‑134 (MQ=255)

           tataCGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTctgcctg                                                                                                                           <  1:721929/134‑1 (MQ=255)

            ataCGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCagag                                                                                                                     <  1:613481/139‑1 (MQ=255)

                  cATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCa                                                                                                               >  1:1031962/1‑139 (MQ=255)

                     ggCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTc                                                                                                            >  1:578634/1‑139 (MQ=255)

                     ggCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAAAGACTTCTGCCTGCCAGAGATCCCAGTc                                                                                                            >  1:1004094/1‑139 (MQ=255)

                         gTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtg                                                                                                        >  1:455253/1‑139 (MQ=255)

                          tGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgc                                                                                                       <  1:784254/139‑1 (MQ=255)

                          tGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgc                                                                                                       >  2:1368485/1‑139 (MQ=255)

                           gAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgct                                                                                                      >  2:37227/1‑139 (MQ=255)

                            aaCGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgctg                                                                                                     >  1:1271988/1‑139 (MQ=255)

                               gTTCCATCCTGGTTGGGGAACACGCCGCTCACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGt                                                                                                  <  1:187092/139‑1 (MQ=255)

                               gTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGt                                                                                                  <  2:1271988/139‑1 (MQ=255)

                                 tCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTaa                                                                                                >  2:973277/1‑139 (MQ=255)

                                        tGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACtt                                                                                                                                  <  2:702509/98‑1 (MQ=255)

                                        tGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACtt                                                                                                                                  >  1:702509/1‑98 (MQ=255)

                                         ggTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGca                                                                                        <  1:1368485/139‑1 (MQ=255)

                                          gTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGcac                                                                                       >  2:1343068/1‑139 (MQ=255)

                                           ttGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGcaca                                                                                      >  1:288444/1‑139 (MQ=255)

                                              gggAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTTCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGcc                                                                                   <  1:1042266/139‑1 (MQ=255)

                                               ggAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCt                                                                                  <  2:288444/139‑1 (MQ=255)

                                                    acGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCa                                                                                                                        >  2:737470/1‑96 (MQ=255)

                                                    acGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCa                                                                                                                        <  1:737470/96‑1 (MQ=255)

                                                    acGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCt                                                                                  >  1:1196520/1‑134 (MQ=255)

                                                    acGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCt                                                                                  <  2:1196520/134‑1 (MQ=255)

                                                      gccgcACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTa                                                                           >  1:630619/1‑139 (MQ=255)

                                                        cgcACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAg                                                                         >  2:186895/1‑139 (MQ=255)

                                                        cgcACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAg                                                                         >  2:647949/1‑139 (MQ=255)

                                                         gcACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGt                                                                        >  1:296817/1‑139 (MQ=255)

                                                          cacaCCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTaaa                                                                                               >  2:871399/1‑115 (MQ=255)

                                                          cacaCCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTaaa                                                                                               <  1:871399/115‑1 (MQ=255)

                                                              ccAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTctgc                                                                                                                              >  1:650442/1‑80 (MQ=255)

                                                              ccAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTctgc                                                                                                                              <  2:650442/80‑1 (MQ=255)

                                                              ccAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATAc                                                                   <  1:186895/139‑1 (MQ=255)

                                                                     ttCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGcaaca                                                            <  1:951628/139‑1 (MQ=255)

                                                                       cGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAAt                                                          >  2:1039339/1‑139 (MQ=255)

                                                                       cGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAAt                                                          <  1:37227/139‑1 (MQ=255)

                                                                         ttgttgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATcc                                                        >  1:1378560/1‑139 (MQ=255)

                                                                            ttgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATc                                                     <  1:1039339/139‑1 (MQ=255)

                                                                            ttgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATc                                                     <  2:455253/139‑1 (MQ=255)

                                                                             tgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTctgcc                                                                                                                             >  1:559186/1‑66 (MQ=255)

                                                                             tgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTctgcc                                                                                                                             <  2:559186/66‑1 (MQ=255)

                                                                             tgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCa                                                    >  2:1133839/1‑139 (MQ=255)

                                                                                    gTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGtt                                                                              <  1:1274942/106‑1 (MQ=255)

                                                                                    gTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGtt                                                                              >  2:1274942/1‑106 (MQ=255)

                                                                                    gTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTg                                             <  1:973277/139‑1 (MQ=255)

                                                                                                     tGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGc                                                                                             >  2:1230355/1‑74 (MQ=255)

                                                                                                     tGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGc                                                                                             <  1:1230355/74‑1 (MQ=255)

                                                                                                      gCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCAc                           <  1:1133839/139‑1 (MQ=255)

                                                                                                           tctcACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCt                      <  1:647949/139‑1 (MQ=255)

                                                                                                              cacaTCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTAt                   <  2:296817/139‑1 (MQ=255)

                                                                                                               acaTCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGc                                                                                    <  1:670805/73‑1 (MQ=255)

                                                                                                               acaTCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGc                                                                                    >  2:670805/1‑73 (MQ=255)

                                                                                                               acaTCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTAtc                  >  1:476483/1‑139 (MQ=255)

                                                                                                                  tCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTAtctct               <  2:630619/139‑1 (MQ=255)

                                                                                                                      gttgCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAAt                                                          >  2:523394/1‑92 (MQ=255)

                                                                                                                      gttgCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAAt                                                          <  1:523394/92‑1 (MQ=255)

                                                                                                                       ttgCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCt          >  1:1066877/1‑139 (MQ=255)

                                                                                                                           tACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAacta                                        >  2:406910/1‑105 (MQ=255)

                                                                                                                           tACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAacta                                        <  1:406910/105‑1 (MQ=255)

                                                                                                                              gTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAACACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGcc   >  1:643124/1‑139 (MQ=255)

                                                                                                                               tataACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCg  >  2:1245717/1‑139 (MQ=255)

TTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCG  >  NC_000913/1419213‑1419478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A