Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A3 F1 I2 R1 | 777 | 59.2 | 2955938 | 92.5% | 2734242 | 106.9 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,494,973 | G→A | *92* (TAG→TAA) | ypdI → | putative lipoprotein involved in colanic acid biosynthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,494,973 | 0 | G | A | 100.0% | 241.7 / NA | 74 | *92* (TAG→TAA) | ypdI | putative lipoprotein involved in colanic acid biosynthesis |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (36/38); total (36/38) | |||||||||||
ACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGA > NC_000913/2494838‑2495107 | acacTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGt < 2:1119786/139‑1 (MQ=255) tACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTca > 1:1014853/1‑139 (MQ=255) tACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTca > 1:86378/1‑139 (MQ=255) tACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTca > 2:933570/1‑139 (MQ=255) aaCTTCAGCCTTCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATgg < 2:1259210/139‑1 (MQ=255) aaCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATgg > 2:1047972/1‑139 (MQ=255) ttCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg > 1:716789/1‑139 (MQ=255) ttCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg > 1:1261508/1‑139 (MQ=255) cAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGt > 2:1089789/1‑139 (MQ=255) aGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTg < 2:86378/139‑1 (MQ=255) gCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGa < 1:348140/139‑1 (MQ=255) ccATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGaa < 2:1163748/139‑1 (MQ=255) ccATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGaa < 1:933570/139‑1 (MQ=255) gCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGt > 1:206941/1‑139 (MQ=255) gATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTct < 1:560078/139‑1 (MQ=255) aTTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctg > 1:145647/1‑139 (MQ=255) ttttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctga < 1:263224/139‑1 (MQ=255) ttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatg < 1:12960/139‑1 (MQ=255) gTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGActgatgct < 1:1247537/139‑1 (MQ=255) tttAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctg < 2:188770/139‑1 (MQ=255) gATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgtg < 2:206941/139‑1 (MQ=255) aagaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTg < 1:1089789/139‑1 (MQ=255) gcgcCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCaa < 2:1086188/139‑1 (MQ=255) ccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGc < 1:1047972/139‑1 (MQ=255) cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGaa > 1:510992/1‑86 (MQ=255) cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGaa < 2:510992/86‑1 (MQ=255) cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg > 1:600214/1‑127 (MQ=255) cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg < 2:600214/127‑1 (MQ=255) cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAccc > 2:1290942/1‑139 (MQ=255) cacaGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAccc > 2:81988/1‑139 (MQ=255) agaaATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTAcc < 2:1261508/139‑1 (MQ=255) aaaTGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGc > 1:1129122/1‑139 (MQ=255) tGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg > 2:1336861/1‑116 (MQ=255) tGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg < 1:1336861/116‑1 (MQ=255) ccgccCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGcc > 2:370790/1‑139 (MQ=255) ctcccccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCTTGACCCATTACCGCCTGCCa < 1:864354/137‑1 (MQ=255) gccCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctg < 2:1420187/83‑1 (MQ=255) gccCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctg > 1:1420187/1‑83 (MQ=255) ccccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATAc > 2:1474713/1‑139 (MQ=255) ccccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATAc > 1:1344610/1‑139 (MQ=255) ccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATAccc < 2:1129122/139‑1 (MQ=255) aGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGa < 2:716789/139‑1 (MQ=255) ggCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCAt < 2:613416/131‑1 (MQ=255) ggCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCAt > 1:613416/1‑131 (MQ=255) tCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTAcc < 2:1111751/103‑1 (MQ=255) tCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTAcc > 1:1111751/1‑103 (MQ=255) ttATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCca < 2:1385036/139‑1 (MQ=255) ttCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACaa > 1:403429/1‑139 (MQ=255) aTCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGcc > 2:1074936/1‑139 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg > 2:706392/1‑75 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg < 1:706392/75‑1 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATcc < 1:486547/81‑1 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATcc > 2:486547/1‑81 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTg < 2:1471660/83‑1 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTg > 1:1471660/1‑83 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAc < 2:1134179/85‑1 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAc > 1:1134179/1‑85 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCa > 2:1451299/1‑88 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCa < 1:1451299/88‑1 (MQ=255) ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCgg > 2:1403677/1‑139 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCAt > 2:224588/1‑37 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCAt < 1:224588/37‑1 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctg > 2:948636/1‑48 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctg < 1:948636/48‑1 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAac > 1:149376/1‑56 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAac < 2:149376/56‑1 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGc < 2:51956/72‑1 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGc > 1:51956/1‑72 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCat < 2:416627/85‑2 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCat > 1:416627/1‑84 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAatcat > 2:1010086/1‑120 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAatcat < 1:1010086/120‑1 (MQ=255) tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCAGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCGAGCCACAAGCCGGGGCa > 2:905611/1‑139 (MQ=255) gATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGa < 2:145647/139‑1 (MQ=255) | ACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGA > NC_000913/2494838‑2495107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |