Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,301,599	G→A	G39S (GGC→AGC)	yhbU →	U32 peptidase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,301,599	0	G	A	100.0%	113.8 / NA	37	G39S (GGC→AGC)	yhbU	U32 peptidase family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/18); total (19/18)

GCGCAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGT  >  NC_000913/3301464‑3301726

gcgcAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGc                                                                                                                               >  2:936876/1‑138 (MQ=255)

                            tgctCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGATAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGa                                                                                                  <  1:356716/139‑1 (MQ=255)

                              ctctGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAg                                                                                                >  1:1176023/1‑139 (MQ=255)

                              ctctGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAg                                                                                                >  2:95838/1‑139 (MQ=255)

                                   ccctgccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTg                                                                                           >  2:585914/1‑139 (MQ=255)

                                        ccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                      >  2:1317334/1‑139 (MQ=255)

                                                   ccGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa                                                                                                 <  1:313513/117‑1 (MQ=255)

                                                   ccGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa                                                                                                 >  2:313513/1‑117 (MQ=255)

                                                    cGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACa                                                                          <  1:1317334/139‑1 (MQ=255)

                                                     ggCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCTTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACAt                                                                         <  2:1286042/139‑1 (MQ=255)

                                                                       cGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATc                                                       <  1:379509/139‑1 (MQ=255)

                                                                            aCGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGAt                                                  <  1:1292934/139‑1 (MQ=255)

                                                                                cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTATCGAGAAAAAATTGCAGGAAGCGGt                                                                                            >  2:180434/1‑93 (MQ=255)

                                                                                cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGt                                                                                            <  1:180434/93‑1 (MQ=255)

                                                                                        cTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTgc                                      >  2:1106075/1‑139 (MQ=255)

                                                                                                     gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgcc                                                                     >  2:561688/1‑95 (MQ=255)

                                                                                                     gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgcc                                                                     <  1:561688/95‑1 (MQ=255)

                                                                                                     gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGt                         >  2:502902/1‑139 (MQ=255)

                                                                                                                atACCAATGCCCGTCACTACGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCAGTTg              >  2:970003/1‑139 (MQ=255)

                                                                                                                     aaTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgc         <  1:95838/139‑1 (MQ=255)

                                                                                                                      aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcg        >  1:712132/1‑139 (MQ=255)

                                                                                                                      aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTCTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcg        <  1:891484/139‑1 (MQ=255)

                                                                                                                       tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtttt                                                                                     <  2:515910/61‑1 (MQ=255)

                                                                                                                       tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtttt                                                                                     >  1:515910/1‑61 (MQ=255)

                                                                                                                           cGTCACTTCGCCACCCTTAACTTCACCGAGAAAAAATTGCAGGAAGCGGTGAGTCTTGTCCATCAACATCGCCGCAAACATCACCTCGCGATTAACACTGTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCg   <  1:970003/139‑1 (MQ=255)

                                                                                                                            gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGcc    >  2:380392/1‑137 (MQ=255)

                                                                                                                            gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGcc    <  1:380392/137‑1 (MQ=255)

                                                                                                                            gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGt  <  1:502902/139‑1 (MQ=255)

                                                                                                                            gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGt  >  1:257941/1‑139 (MQ=255)

                                                                                                                             tCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTg                                                                                    <  2:295122/56‑1 (MQ=255)

                                                                                                                             tCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTg                                                                                    >  1:295122/1‑56 (MQ=255)

                                                                                                                              cACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGt                                                                                            >  2:176970/1‑47 (MQ=255)

                                                                                                                              cACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGt                                                                                            <  1:176970/47‑1 (MQ=255)

                                                                                                                              cACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACa                                                                          >  1:680646/1‑65 (MQ=255)

                                                                                                                              cACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACa                                                                          <  2:680646/65‑1 (MQ=255)

                                                                                                                                 ttCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcg        >  2:1108617/1‑128 (MQ=255)

                                                                                                                                 ttCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcg        <  1:1108617/128‑1 (MQ=255)

GCGCAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGT  >  NC_000913/3301464‑3301726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A