Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,610,816	T→C	V468A (GTT→GCT)	prfC →	peptide chain release factor RF‑3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,610,816	0	T	C	98.7%	269.4 / NA	76	V468A (GTT→GCT)	prfC	peptide chain release factor RF‑3
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (40/35); minor base A (1/0); total (41/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCGGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTGCGCC  >  NC_000913/4610681‑4610950

gcgcGGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGc                                                                                                                                     >  2:939458/1‑139 (MQ=255)

 cgcgGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGcc                                                                                                                                    >  1:41571/1‑139 (MQ=255)

      tGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGc                                                                                                                               <  2:41571/139‑1 (MQ=255)

        cAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                             <  2:1442197/139‑1 (MQ=255)

         aGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCg                                                                                                                            <  2:663869/139‑1 (MQ=255)

          ggTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGc                                                                                                                           <  2:721707/139‑1 (MQ=255)

          ggTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGc                                                                                                                           <  1:937304/139‑1 (MQ=255)

          ggTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGc                                                                                                                           >  1:721707/1‑139 (MQ=255)

              ttCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTggg                                                                                                                       <  2:1352042/139‑1 (MQ=255)

                ccGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTa                                                                                                                     >  1:582884/1‑139 (MQ=255)

                ccGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTa                                                                                                                     >  1:29528/1‑139 (MQ=255)

                                    aTCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTa                                                                                                                     <  1:661396/119‑1 (MQ=255)

                                    aTCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTa                                                                                                                     >  2:661396/1‑119 (MQ=255)

                                    aTCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCa                                                                                                            >  1:1466057/1‑128 (MQ=255)

                                    aTCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCa                                                                                                            <  2:1466057/128‑1 (MQ=255)

                                    aTCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCgaagaa                                                                                                 >  2:1470325/1‑139 (MQ=255)

                                    aTCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCgaagaa                                                                                                 >  2:415002/1‑139 (MQ=255)

                                    aTCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCgaagaa                                                                                                 >  2:647135/1‑139 (MQ=255)

                                    aTCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCgaagaa                                                                                                 >  1:764029/1‑139 (MQ=255)

                                      cTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCg                                                                                                                            <  1:396471/110‑1 (MQ=255)

                                      cTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCg                                                                                                                            >  2:396471/1‑110 (MQ=255)

                                           cGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGaa                                                                                          <  1:1353899/139‑1 (MQ=255)

                                           cGTTGGTGCAGTTGGTGTGCTGCAGGTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGaa                                                                                          <  1:939458/139‑1 (MQ=255)

                                              tGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                             <  1:970089/101‑1 (MQ=255)

                                              tGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                             >  2:970089/1‑101 (MQ=255)

                                               ggTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGt                                                                                      >  2:793885/1‑139 (MQ=255)

                                               ggTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGt                                                                                      >  2:418054/1‑139 (MQ=255)

                                               ggTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTCAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCgaa                                                                                                    >  2:936970/1‑125 (MQ=255)

                                               ggTGCAGTTGGTGTGCTGAAGTTTGATGTGGAGGTAGCGCGACTGAAGAGCGAAAACAACGTTGAAGCAGTGAATGAGTCAGTCAACGATGCCAGTGCCCGCTGGGTAGAATGGGCAGACGAGAAGAAAATGGAAGAGt                                                                                      >  1:85283/1‑139 (MQ=255)

                                               ggTGCAGTTGGTCTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCgaa                                                                                                    <  1:936970/125‑1 (MQ=255)

                                                 tGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTc                                                                                    >  1:598114/1‑139 (MQ=255)

                                                 tGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTc                                                                                    <  1:1470325/139‑1 (MQ=255)

                                                  gCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCa                                                                                   <  2:764029/139‑1 (MQ=255)

                                                   cAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGAc                                                                                                         <  1:1318673/116‑1 (MQ=255)

                                                   cAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGAc                                                                                                         >  2:1318673/1‑116 (MQ=255)

                                                        ggTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCTCTGCCCGCTggg                                                                                                                       <  2:681688/97‑1 (MQ=255)

                                                        ggTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTggg                                                                                                                       >  1:681688/1‑97 (MQ=255)

                                                        ggTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTa                                                                             <  1:415002/139‑1 (MQ=255)

                                                                gcAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGc                                                                                                                               <  1:1240441/81‑1 (MQ=255)

                                                                gcAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGc                                                                                                                               >  2:1240441/1‑81 (MQ=255)

                                                                 cAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGaaa                                                                    >  1:909912/1‑139 (MQ=255)

                                                                   gTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGc                                                                  <  2:598114/139‑1 (MQ=255)

                                                                      tGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGaa                                                                                          >  2:87936/1‑112 (MQ=255)

                                                                      tGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGaa                                                                                          <  1:87936/112‑1 (MQ=255)

                                                                       gATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAAc                                                              <  1:793885/139‑1 (MQ=255)

                                                                             gtggtAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGgcgc                                                        >  2:266977/1‑139 (MQ=255)

                                                                                gtAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTg                                                     >  1:1212225/1‑139 (MQ=255)

                                                                                        ccTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                             <  2:120213/59‑1 (MQ=255)

                                                                                        ccTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                             >  1:120213/1‑59 (MQ=255)

                                                                                        ccTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATggcggc                                             >  2:1362971/1‑139 (MQ=255)

                                                                                        ccTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATggcggc                                             >  1:425463/1‑139 (MQ=255)

                                                                                          tGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGa                                           <  2:371132/139‑1 (MQ=255)

                                                                                           gAAGAGCGAATACAACGTTGAAGCAATGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGAt                                          >  1:63189/1‑139 (MQ=255)

                                                                                              gagCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAAc                                       <  1:266977/139‑1 (MQ=255)

                                                                                                 cGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTc                                    >  2:267563/1‑139 (MQ=255)

                                                                                                   aaTACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATg                                                  <  1:873962/123‑1 (MQ=255)

                                                                                                   aaTACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGc                                  >  2:25362/1‑139 (MQ=255)

                                                                                                   aaTACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTACAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATg                                                  >  2:873962/1‑123 (MQ=255)

                                                                                                        aaCGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACa                             <  2:909912/139‑1 (MQ=255)

                                                                                                         aCGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACAt                            <  2:1212225/139‑1 (MQ=255)

                                                                                                         aCGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACAt                            <  1:25362/139‑1 (MQ=255)

                                                                                                         aCGTTGAAGCAATGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACAt                            <  2:63189/139‑1 (MQ=255)

                                                                                                          cGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTgg                                                                                                                        >  2:108259/1‑46 (MQ=255)

                                                                                                          cGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTgg                                                                                                                        <  1:108259/46‑1 (MQ=255)

                                                                                                          cGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACAt                            >  2:635093/1‑138 (MQ=255)

                                                                                                          cGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACAt                            <  1:635093/138‑1 (MQ=255)

                                                                                                             tGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCt                        >  1:1098684/1‑139 (MQ=255)

                                                                                                                            gtcagtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCt                        <  2:503915/124‑1 (MQ=255)

                                                                                                                            gtcagtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCt                        >  1:503915/1‑124 (MQ=255)

                                                                                                                               agtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAAc                                       >  2:41457/1‑106 (MQ=255)

                                                                                                                               agtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAAc                                       <  1:41457/106‑1 (MQ=255)

                                                                                                                               agtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTg      >  2:1126414/1‑139 (MQ=255)

                                                                                                                               agtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTg      >  1:38338/1‑139 (MQ=255)

                                                                                                                               agtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTg      >  1:1221956/1‑139 (MQ=255)

                                                                                                                                 tcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTgcg    >  2:206314/1‑139 (MQ=255)

                                                                                                                                   aaCGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTGCGcc  <  2:1098684/139‑1 (MQ=255)

GCGCGGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTGCGCC  >  NC_000913/4610681‑4610950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A