Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	515,808	C→T	intergenic (‑35/‑111)	qmcA ← / → fetA	PHB domain membrane‑anchored putative protease/iron export ABC transporter ATPase, peroxide resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	515,808	0	C	T	100.0%	147.3 / NA	46	intergenic (‑35/‑111)	qmcA/fetA	PHB domain membrane‑anchored putative protease/iron export ABC transporter ATPase, peroxide resistance protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/23); total (23/23)

GTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAG  >  NC_000913/515685‑515924

gTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGa                                                                                                       <  1:1614931/139‑1 (MQ=255)

        aTAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACaa                                                                                               >  2:1318873/1‑139 (MQ=255)

           tcccTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAAtt                                                                                            >  2:812094/2‑139 (MQ=255)

           gCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAAtt                                                                                            >  1:100521/1‑139 (MQ=255)

              cTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATtata                                                                                         <  2:100521/139‑1 (MQ=255)

                gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATAcc                                                                                       >  1:535872/1‑139 (MQ=255)

                  ggTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTg                                                                                     >  2:758972/1‑139 (MQ=255)

                  ggTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTg                                                                                     >  1:366714/1‑139 (MQ=255)

                    tACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGAt                                                                                   <  1:270427/139‑1 (MQ=255)

                                   cgcgCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGtt                                                                    >  2:627381/1‑139 (MQ=255)

                                   cgcgCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGtt                                                                    <  1:197193/139‑1 (MQ=255)

                                   cgcgCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGtt                                                                    <  1:656820/139‑1 (MQ=255)

                                     cgcCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTcc                                                                  >  1:1001644/1‑139 (MQ=255)

                                           cAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTa                                                            <  1:758972/139‑1 (MQ=255)

                                           cAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTa                                                            <  1:1318873/139‑1 (MQ=255)

                                                aCCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCt                                                       >  2:575841/1‑139 (MQ=255)

                                                         aCAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGAt                                              <  1:627381/139‑1 (MQ=255)

                                                          cAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTaaa                                                                                                  >  2:84943/1‑86 (MQ=255)

                                                          cAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTaaa                                                                                                  <  1:84943/86‑1 (MQ=255)

                                                          cAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATa                                             >  2:1482913/1‑139 (MQ=255)

                                                                    aaTCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAagaga                                                                       <  2:437935/103‑1 (MQ=255)

                                                                    aaTCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAagaga                                                                       >  1:437935/1‑103 (MQ=255)

                                                                       cGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTACCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGt                                <  2:1001644/139‑1 (MQ=255)

                                                                                    agcagcGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAAt                                                                                                                 <  2:591178/45‑1 (MQ=255)

                                                                                    aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAAt                                                                                                                 >  1:591178/1‑45 (MQ=255)

                                                                                    aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTc                                                         >  1:1230472/1‑101 (MQ=255)

                                                                                    aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTc                                                         <  2:1230472/101‑1 (MQ=255)

                                                                                    aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGaaaa                   >  1:65870/1‑139 (MQ=255)

                                                                                    aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGaaaa                   >  1:555654/1‑139 (MQ=255)

                                                                                     gCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGaaaaa                  <  2:1170787/139‑1 (MQ=255)

                                                                                        tCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAggg               <  2:65870/139‑1 (MQ=255)

                                                                                           gAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAAc            >  1:315364/1‑139 (MQ=255)

                                                                                             aaaaCCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAg          <  1:1482913/139‑1 (MQ=255)

                                                                                                aCCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGAt                                              >  2:476270/1‑100 (MQ=255)

                                                                                                aCCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGAt                                              <  1:476270/100‑1 (MQ=255)

                                                                                                     ctGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                   >  2:1062670/1‑90 (MQ=255)

                                                                                                     ctGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                   <  1:1062670/90‑1 (MQ=255)

                                                                                                     ctGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAg  <  2:555654/139‑1 (MQ=255)

                                                                                                       gttgtACCGTCCATAATCAGTAAAATTGCTGCTTGAtt                                                                                                     >  1:738597/1‑38 (MQ=38)

                                                                                                       gttgtACCGTCCATAATCAGTAAAATTGCTGCTTGAtt                                                                                                     <  2:738597/38‑1 (MQ=255)

                                                                                                                tCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGtt                                                                    >  2:1390373/1‑62 (MQ=255)

                                                                                                                tCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGtt                                                                    <  1:1390373/62‑1 (MQ=255)

                                                                                                                tCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTa                                                            >  2:387556/1‑70 (MQ=255)

                                                                                                                tCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTa                                                            <  1:387556/70‑1 (MQ=255)

                                                                                                                       tCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                   >  2:348234/1‑72 (MQ=255)

                                                                                                                       tCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                   <  1:348234/72‑1 (MQ=255)

GTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAG  >  NC_000913/515685‑515924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A