Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,006,415	Δ1 bp	coding (464/543 nt)	zapC →	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,006,413	0	G	.	100.0%	205.7 / NA	50	coding (462/543 nt)	zapC	FtsZ stabilizer
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (24/26); total (24/26)

CCGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGC  >  NC_000913/1006276‑1006546

ccGGGCGATGCGGCCTGCGTTTGGTTGAGCGAAACTCACGTGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTTGCACAGCCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTgg                                                                                                                                      <  2:1079404/139‑1 (MQ=255)

   ggCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcga                                                                                                                                   <  1:1248573/139‑4 (MQ=255)

     cGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACg                                                                                                                                <  2:881020/139‑1 (MQ=255)

                        ttGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                             <  2:323529/139‑1 (MQ=255)

                           aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAg                                                                                                          >  1:1378846/1‑139 (MQ=255)

                           aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAg                                                                                                          >  1:271297/1‑139 (MQ=255)

                                tACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGa                                                                                                     >  2:684330/1‑139 (MQ=255)

                                     aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                >  2:581375/1‑139 (MQ=255)

                                       gAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCa                                                                                              >  2:1193622/1‑139 (MQ=255)

                                          cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  2:652533/1‑139 (MQ=255)

                                          cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  2:5142/1‑139 (MQ=255)

                                            ggTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                         >  2:829767/1‑139 (MQ=255)

                                            ggTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                         <  1:581375/139‑1 (MQ=255)

                                             gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  1:198276/1‑136 (MQ=255)

                                             gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           <  2:198276/136‑1 (MQ=255)

                                                  tttGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           <  2:759585/131‑1 (MQ=255)

                                                  tttGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  1:759585/1‑131 (MQ=255)

                                                    tGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 <  1:70483/139‑1 (MQ=255)

                                                         gTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCtt                                                                            <  2:1378846/139‑1 (MQ=255)

                                                         gTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCtt                                                                            <  1:829767/139‑1 (MQ=255)

                                                            gTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaa                                                                                                                        <  2:1038153/92‑1 (MQ=255)

                                                            gTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaa                                                                                                                        >  1:1038153/1‑92 (MQ=255)

                                                            gTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAg                                                                         >  1:413982/1‑139 (MQ=255)

                                                                                   aCTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAAcgcg                                                  <  1:5142/139‑1 (MQ=255)

                                                                                       tGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCaa                                              >  2:659573/1‑139 (MQ=255)

                                                                                              tgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                             <  1:1333608/69‑1 (MQ=255)

                                                                                              tgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                             >  2:1333608/1‑69 (MQ=255)

                                                                                               ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaaa                                                                                                                       >  1:278764/1‑58 (MQ=255)

                                                                                               ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaaa                                                                                                                       <  2:278764/58‑1 (MQ=255)

                                                                                               ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                                      <  2:413982/139‑1 (MQ=255)

                                                                                               ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                                      <  2:1500101/139‑1 (MQ=255)

                                                                                                   caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                  <  1:684330/139‑1 (MQ=255)

                                                                                                      cccTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                               <  1:652533/139‑1 (MQ=255)

                                                                                                        cTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTgg                                                                                                                                      <  2:654186/35‑1 (MQ=255)

                                                                                                        cTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTgg                                                                                                                                      >  1:654186/1‑35 (MQ=255)

                                                                                                         tGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  1:562737/71‑1 (MQ=255)

                                                                                                         tGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                >  2:562737/1‑71 (MQ=255)

                                                                                                         tGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCgg                            <  1:1193622/139‑1 (MQ=255)

                                                                                                                ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 <  2:184874/79‑1 (MQ=255)

                                                                                                                ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 >  1:184874/1‑79 (MQ=255)

                                                                                                                     gCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACa                >  1:847432/1‑139 (MQ=255)

                                                                                                                        ggTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTTATGTTGa                                                                                  >  1:1054273/1‑70 (MQ=255)

                                                                                                                        ggTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa                                                                                  <  2:1054273/70‑1 (MQ=255)

                                                                                                                        ggTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa             >  1:18232/1‑139 (MQ=255)

                                                                                                                          tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa             <  2:188963/137‑1 (MQ=255)

                                                                                                                          tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa             >  1:188963/1‑137 (MQ=255)

                                                                                                                             cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                                      >  2:712540/1‑109 (MQ=255)

                                                                                                                             cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                                      <  1:712540/109‑1 (MQ=255)

                                                                                                                             cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                  <  1:10332/113‑1 (MQ=255)

                                                                                                                             cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                  >  2:10332/1‑113 (MQ=255)

                                                                                                                             cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGaa        <  2:257564/139‑1 (MQ=255)

                                                                                                                                  tGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                               >  2:332285/1‑111 (MQ=255)

                                                                                                                                  tGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                               <  1:332285/111‑1 (MQ=255)

                                                                                                                                   gCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGc  <  2:18232/139‑1 (MQ=255)

CCGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGC  >  NC_000913/1006276‑1006546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A