Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I2 R1 766 64.2 3232166 90.9% 2938038 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,146,011 C→T *195* (TAG→TAA)  yceF ← m(7)GTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,146,0110CT100.0% 202.2 / NA 63*195* (TAG→TAA) yceFm(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/30);  total (33/30)

CTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTC  >  NC_000913/1145882‑1146136
                                                                                                                                 |                                                                                                                             
cTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATc                                                                                                                      >  1:1270584/1‑139 (MQ=255)
         gcgcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGttt                                                                                                             >  1:1504968/1‑139 (MQ=255)
                         atatAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                                                             <  2:725375/139‑1 (MQ=255)
                         atatAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                                                             >  2:670895/1‑139 (MQ=255)
                                 cTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGt                                                                                              <  2:222372/130‑1 (MQ=255)
                                 cTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGt                                                                                              >  1:222372/1‑130 (MQ=255)
                                     tAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGAcaca                                                                                 >  1:958459/1‑139 (MQ=255)
                                     tAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGAcaca                                                                                 <  2:1442097/139‑1 (MQ=255)
                                     tAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGAcaca                                                                                 >  1:532804/1‑139 (MQ=255)
                                           gCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCt                                                                                       >  2:1403516/1‑127 (MQ=255)
                                           gCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCt                                                                                       <  1:1403516/127‑1 (MQ=255)
                                           gCGGTTGGCCTGATAAGGCGTTTACGCCGCATAAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGa                                                                           >  1:957980/1‑139 (MQ=255)
                                           gCGGTTGGCCTAATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGa                                                                           >  1:1170098/1‑139 (MQ=255)
                                            cGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGAt                                                                          <  1:474786/139‑1 (MQ=255)
                                             ggTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGAtt                                                                         <  1:670895/139‑1 (MQ=255)
                                              gTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                                                             >  2:81059/1‑118 (MQ=255)
                                              gTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                                                             <  1:81059/118‑1 (MQ=255)
                                                       aTAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAaac                                                               >  1:1052918/1‑139 (MQ=255)
                                                             cGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAaccaacca                                                         <  1:400801/139‑1 (MQ=255)
                                                             cGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAaccaacca                                                         <  2:1504968/139‑1 (MQ=255)
                                                               tttACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGc                                                       <  2:532804/139‑1 (MQ=255)
                                                                 tACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                                                             >  1:1362897/1‑99 (MQ=255)
                                                                 tACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                                                             <  2:1362897/99‑1 (MQ=255)
                                                                 tACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCgt                                                     >  1:1341656/1‑139 (MQ=255)
                                                                 tACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCgt                                                     >  1:415103/1‑139 (MQ=255)
                                                                 tACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCgt                                                     >  2:1301005/1‑139 (MQ=255)
                                                                      cgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACAt                                                                                         <  1:158854/98‑1 (MQ=255)
                                                                      cgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACAt                                                                                         >  2:158854/1‑98 (MQ=255)
                                                                      cgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGAt                                                                          <  2:942314/113‑1 (MQ=255)
                                                                      cgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGAt                                                                          >  1:942314/1‑113 (MQ=255)
                                                                      cgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAg                                                        <  1:1352768/131‑1 (MQ=255)
                                                                      cgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAg                                                        >  2:1352768/1‑131 (MQ=255)
                                                                        cATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAg                                                                                                                    >  2:471023/1‑69 (MQ=255)
                                                                        cATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAg                                                                                                                    <  1:471023/69‑1 (MQ=255)
                                                                           cAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCa                                           <  1:1301005/139‑1 (MQ=255)
                                                                            aGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCAc                                          <  2:812492/139‑1 (MQ=255)
                                                                                  ccAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAg                                                        <  1:1037633/119‑1 (MQ=255)
                                                                                  ccAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAg                                                        >  2:1037633/1‑119 (MQ=255)
                                                                                     gCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTa                                 <  1:1088073/139‑1 (MQ=255)
                                                                                     gCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTa                                 <  2:551096/139‑1 (MQ=255)
                                                                                        ccGATTGCTGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTACCATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAAc                              <  2:957980/139‑1 (MQ=255)
                                                                                        ccGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTttcccttgcc                                                                                                   >  1:940751/1‑70 (MQ=255)
                                                                                        ccGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTttcccttccc                                                                                                   <  2:940751/70‑1 (MQ=255)
                                                                                          gATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGc                            >  1:385461/1‑139 (MQ=255)
                                                                                           aTTGTCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAaaccaac                                                           >  2:924894/1‑107 (MQ=255)
                                                                                           aTTGTCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAaaccaac                                                           <  1:924894/107‑1 (MQ=255)
                                                                                           aTTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCt                           >  1:682752/1‑139 (MQ=255)
                                                                                            ttGCCGGATGCGACGTAACCACATCCGGTGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTc                          >  2:1450629/1‑139 (MQ=255)
                                                                                                             aCCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGAt                                                                          <  1:823287/74‑1 (MQ=255)
                                                                                                             aCCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGAt                                                                          >  2:823287/1‑74 (MQ=255)
                                                                                                                  aTCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCt    >  2:599736/1‑139 (MQ=255)
                                                                                                                   tCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCaaa                       <  2:612364/119‑1 (MQ=255)
                                                                                                                   tCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCaaa                       >  1:612364/1‑119 (MQ=255)
                                                                                                                    ccGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGCCACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACg                                         <  1:1141458/100‑1 (MQ=255)
                                                                                                                    ccGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACg                                         >  2:1141458/1‑100 (MQ=255)
                                                                                                                       gcgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTc  >  2:661344/1‑136 (MQ=255)
                                                                                                                       gcgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTc  <  1:661344/136‑1 (MQ=255)
                                                                                                                        cgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                                                             >  2:829377/1‑44 (MQ=255)
                                                                                                                        cgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                                                             <  1:829377/44‑1 (MQ=255)
                                                                                                                        cgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAaaccaac                                                           >  1:1450996/1‑78 (MQ=255)
                                                                                                                        cgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAaaccaac                                                           <  2:1450996/78‑1 (MQ=255)
                                                                                                                        cgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACa                     >  1:839119/1‑116 (MQ=255)
                                                                                                                        cgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACa                     <  2:839119/116‑1 (MQ=255)
                                                                                                                                 |                                                                                                                             
CTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTC  >  NC_000913/1145882‑1146136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A