Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,940,048	G→A	T227T (ACG→ACA)	fucR →	l‑fucose operon activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,940,048	0	G	A	100.0%	179.7 / NA	55	T227T (ACG→ACA)	fucR	l‑fucose operon activator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/23); total (32/23)

CAATGCGATCAACGCTGATTACAAATCGATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACGCACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTCGCGACGACCACCCACCGCAGCCCAGA  >  NC_000913/2939922‑2940180

cAATGCGATCAACGCTGATTACAAATCGATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCt                                                                                                                          >  1:850462/1‑139 (MQ=255)

  aTGCGATCAACGCTGATTACAAATCGATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCtga                                                                                                                        >  2:188129/1‑139 (MQ=255)

  aTGCGATCAACGCTGATTACAAATCGATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCtga                                                                                                                        >  2:759056/1‑139 (MQ=255)

   tGCGATCAACGCTGATTACAAATCGATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCtgat                                                                                                                       <  1:1226189/139‑1 (MQ=255)

       aTCAACGCTGATTACAAATCGATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAgc                                                                                                                   >  1:123024/1‑139 (MQ=255)

             gCTGATTACAAATCGATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAgg                                                                                                             <  2:123024/139‑1 (MQ=255)

                         tCGATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAActtctt                                                                                                 <  2:323207/139‑1 (MQ=255)

                           gATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACat                                                                                                                                 <  1:746323/105‑1 (MQ=255)

                           gATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACat                                                                                                                                 >  2:746323/1‑105 (MQ=255)

                                        cGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGAc                                                                                  >  1:66489/1‑139 (MQ=255)

                                         gTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACg                                                                                 >  2:1410568/1‑139 (MQ=255)

                                          tGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGATCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACATCTTTGGTAACCGACTGACgg                                                                                >  1:807880/1‑139 (MQ=255)

                                           gCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGc                                                                                                         >  2:613618/1‑113 (MQ=255)

                                           gCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGc                                                                                                         <  1:613618/113‑1 (MQ=255)

                                            ccGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGaa                                                                              >  2:706001/1‑139 (MQ=255)

                                                   aTCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAg                                                                       <  2:850462/139‑1 (MQ=255)

                                                   aTCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAg                                                                       <  1:1410568/139‑1 (MQ=255)

                                                              tgattgatAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAActtct                                                                                                  >  1:156101/1‑101 (MQ=255)

                                                              tgattgatAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAActtct                                                                                                  <  2:156101/101‑1 (MQ=255)

                                                              tgattgatAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTaa                                                                                           <  2:1384326/108‑1 (MQ=255)

                                                              tgattgatAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTaa                                                                                           >  1:1384326/1‑108 (MQ=255)

                                                                attgatAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGATGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCGGGAgacg                                                          >  1:843271/1‑139 (MQ=255)

                                                                  tgatAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGTTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAgacgac                                                        >  1:39694/1‑139 (MQ=255)

                                                                  tgatAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGga                                                              <  2:149306/133‑1 (MQ=255)

                                                                  tgatAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGga                                                              >  1:149306/1‑133 (MQ=255)

                                                                  tgatAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAgacgac                                                        >  1:862001/1‑139 (MQ=255)

                                                                    atAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAg                                                                       <  1:545506/122‑1 (MQ=255)

                                                                    atAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAg                                                                       >  2:545506/1‑122 (MQ=255)

                                                                    atAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGc                                                      >  2:157166/1‑139 (MQ=255)

                                                                    atAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTCGGTAACAGCCTGACGGAAAGGGTAg                                                                       >  2:253295/1‑122 (MQ=255)

                                                                    atAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTCGGTAACAGCCTGACGGAAAGGGTAg                                                                       <  1:253295/122‑1 (MQ=255)

                                                                           tAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGcc                                               <  1:759056/139‑1 (MQ=255)

                                                                           tAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATGTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGcc                                               <  2:807880/139‑1 (MQ=255)

                                                                                  aaTCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAAct                                                                                                     >  1:541377/1‑78 (MQ=255)

                                                                                  aaTCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAAct                                                                                                     <  2:541377/78‑1 (MQ=255)

                                                                                         cAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGc                                 >  2:1214902/1‑139 (MQ=255)

                                                                                         cAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGc                                 >  2:1353182/1‑139 (MQ=255)

                                                                                            gggAAGCCCGCATCGGGCATCTGGATGAGGTAACACACat                                                                                                                                 <  1:472459/40‑1 (MQ=255)

                                                                                            gggAAGCCCGCATCGGGCATCTGGATGAGGTAACACACat                                                                                                                                 >  2:472459/1‑40 (MQ=255)

                                                                                              gAAGCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCtcgtc                            <  1:706001/139‑1 (MQ=255)

                                                                                                 gCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGt                                                                                                            >  2:899655/1‑56 (MQ=255)

                                                                                                 gCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGt                                                                                                            <  1:899655/56‑1 (MQ=255)

                                                                                                 gCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTcgcg                         >  2:1030402/1‑139 (MQ=255)

                                                                                                 gCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTcgcg                         >  2:442364/1‑139 (MQ=255)

                                                                                                 gCCCGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTcgcg                         >  2:403700/1‑139 (MQ=255)

                                                                                                  cccGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCa                                                                     >  2:1061154/1‑94 (MQ=255)

                                                                                                  cccGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCa                                                                     <  1:1061154/94‑1 (MQ=255)

                                                                                                  cccGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTCGcga                        >  2:37609/1‑139 (MQ=255)

                                                                                                  cccGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTCGcga                        >  2:1609489/1‑139 (MQ=255)

                                                                                                  cccGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTCGcga                        >  2:14478/1‑139 (MQ=255)

                                                                                                  cccGCATCGGGCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTCGcga                        <  1:1609489/139‑1 (MQ=255)

                                                                                                          gggCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTCGCGACGaccacc                <  1:37609/139‑1 (MQ=255)

                                                                                                          gggCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTCGCGACGaccacc                <  1:1214902/139‑1 (MQ=255)

                                                                                                           ggCATCTGGATGAGGTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTCGCGACGAccaccc               >  1:642199/1‑139 (MQ=255)

                                                                                                                        ggTAACACACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTCGCGACGACCACCCACCGCAGCCCAGa  <  1:442364/139‑1 (MQ=255)

CAATGCGATCAACGCTGATTACAAATCGATGCTATTAAAACGTGCCGCGCAATCGTTGTTATTGATTGATAAAAGTAAATTTAATCGTTCAGGGGAAGCCCGCATCGGGCATCTGGATGAGGTAACGCACATTATTTCTGATGAGCGCCAGGTTGCAACTTCTTTGGTAACAGCCTGACGGAAAGGGTAGCAGGCCGGAGACGACGCCCCGGCCTTGCCTGTTATCGCTCGTCGCGACGACCACCCACCGCAGCCCAGA  >  NC_000913/2939922‑2940180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A