Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	357,791	C→T	300 (TAG→TAA)	cynR ←	transcriptional activator of cyn operon, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	357,791	0	C	T	100.0%	133.5 / NA	46	300 (TAG→TAA)	cynR	transcriptional activator of cyn operon, autorepressor
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/21); total (25/21)

GGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAA  >  NC_000913/357693‑357924

ggcgTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCg                                                                                                                                  <  2:479797/104‑1 (MQ=255)

ggcgTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCg                                                                                                                                  >  1:479797/1‑104 (MQ=25)

  cgTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCAt                                                                                                      >  2:692605/1‑130 (MQ=255)

  cgTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCAt                                                                                                      <  1:692605/130‑1 (MQ=255)

     gAACGCCTTATCCGGCCTACGTAGAGCACTGAGCTCGTAGGTCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGGGCATTTATCCAACGcc                                                                                          <  1:444337/139‑1 (MQ=11)

     gAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGcc                                                                                          <  2:1051334/139‑1 (MQ=12)

                   gCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGc                                                                            >  1:730086/1‑139 (MQ=255)

                        cGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGc                                                                            >  2:630931/1‑134 (MQ=255)

                        cGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGc                                                                            <  1:630931/134‑1 (MQ=255)

                             agCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCgcagcg                                                                  >  2:670287/1‑139 (MQ=255)

                             agCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCgcggcg                                                                  >  2:704773/1‑139 (MQ=255)

                              gCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGcggcgg                                                                 <  2:425088/139‑1 (MQ=255)

                              gCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGcggcgg                                                                 <  2:418990/139‑1 (MQ=255)

                                  tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGt                                                             >  2:58017/1‑139 (MQ=255)

                                           aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCt                                                    <  1:704773/139‑1 (MQ=255)

                                              ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCg                                                                                                                                  <  1:1053350/58‑1 (MQ=255)

                                              ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCg                                                                                                                                  >  2:1053350/1‑58 (MQ=255)

                                                 gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAttttt                                              >  1:721172/1‑139 (MQ=255)

                                                   tAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTcc                                            >  2:599722/1‑139 (MQ=255)

                                                     aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgc                                          <  2:149966/139‑1 (MQ=255)

                                                       cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGcc                                                                                          >  2:936307/1‑89 (MQ=11)

                                                       cGTAGCGCATCAGGCAATTCCAGCCGCAGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGcc                                                                                          <  1:936307/89‑1 (MQ=255)

                                                       cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgccg                                        >  1:570149/1‑139 (MQ=255)

                                                       cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgccg                                        >  1:1071274/1‑139 (MQ=255)

                                                        gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCcgccgc                                       >  2:168314/1‑139 (MQ=255)

                                                         tAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAAcc                                                                                                            >  1:899975/1‑69 (MQ=255)

                                                         tAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAAcc                                                                                                            <  2:899975/69‑1 (MQ=255)

                                                            cgcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTATTTTTcatccggaata                                   >  1:490878/1‑129 (MQ=11)

                                                            cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGc                                                     <  2:1187610/121‑1 (MQ=255)

                                                            cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGc                                                     >  1:1187610/1‑121 (MQ=255)

                                                            cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca                                   >  1:1097126/1‑139 (MQ=255)

                                                            cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca                                   <  2:570149/139‑1 (MQ=255)

                                                            cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca                                   >  1:1194623/1‑139 (MQ=255)

                                                             gcATCAGGCAATTCCAGCCGCAGACCCGTGTCAGCGGTTACCGTGATTCATTTCCGCAAACAATCGCGCATTTATCCAACGCCATGTGCACAACTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaacaa                                  >  1:1130114/1‑139 (MQ=18)

                                                                  aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaaccgc                             >  1:638297/1‑135 (MQ=255)

                                                                     cAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgccg                          <  2:1194623/139‑1 (MQ=255)

                                                                     cAATTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgccg                          <  1:58017/139‑1 (MQ=255)

                                                                          ccAGCCGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctc                     <  2:490878/139‑1 (MQ=255)

                                                                               cGCAGACGTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACACCAtgt                                                                                      >  1:797945/1‑69 (MQ=18)

                                                                               cGCAGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAtgt                                                                                      <  2:797945/69‑1 (MQ=255)

                                                                                  aGACCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagtag             >  1:624908/1‑139 (MQ=255)

                                                                                         tgtCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGc                                                     <  1:164867/92‑1 (MQ=255)

                                                                                         tgtCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGc                                                     >  2:164867/1‑92 (MQ=255)

                                                                                             aGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCaa  <  1:168314/139‑1 (MQ=255)

                                                                                              gCGGTTACCGTGATTCATTTCCGCCAATAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGcggcgg                                                                 <  1:237411/75‑1 (MQ=17)

                                                                                              gCGGTTACCGTGATTCATTTCCGCCAATAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGcggcgg                                                                 >  2:237411/1‑75 (MQ=17)

GGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAA  >  NC_000913/357693‑357924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A