Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
649,372 |
A→G |
F67S (TTC→TCC) |
citF ← |
citrate lyase, citrate‑ACP transferase (alpha) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 649,372 | 0 | A | G | 98.0%
| 176.2
/ ‑4.6
| 51 | F67S (TTC→TCC) | citF | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (22/28); total (23/28) |
| Fisher's exact test for biased strand distribution p-value = 4.51e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01 |
TTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCA > NC_000913/649245‑649497
|
ttCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTc < 2:867419/139‑1 (MQ=255)
tCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCa > 1:1124952/1‑139 (MQ=255)
tCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCa < 2:491481/139‑1 (MQ=255)
tACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg < 2:329975/139‑1 (MQ=255)
tACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg < 1:449287/139‑1 (MQ=255)
cAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg > 1:828105/1‑139 (MQ=255)
cAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg < 2:598349/139‑1 (MQ=255)
cAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCAGATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGTAACCGTCATGCCg < 1:823060/139‑1 (MQ=255)
gcggcgCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTc > 2:50220/1‑139 (MQ=255)
cATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTaa > 1:1074888/1‑139 (MQ=255)
cgCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAAcc > 1:583488/2‑139 (MQ=255)
tCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGc > 1:1069002/1‑139 (MQ=255)
tCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGc < 2:1069002/139‑1 (MQ=255)
cTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg < 2:614904/120‑1 (MQ=255)
cTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg > 1:614904/1‑120 (MQ=255)
gggAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCCGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAAt > 1:30790/1‑139 (MQ=255)
gCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCa > 2:402337/1‑139 (MQ=255)
cAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCg < 2:647592/95‑1 (MQ=255)
cAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCg > 1:647592/1‑95 (MQ=255)
aGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGgcgc < 2:493534/139‑1 (MQ=255)
ggTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca < 1:50220/139‑1 (MQ=255)
cccATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGAt > 2:965500/1‑139 (MQ=255)
ccATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg > 1:1180306/1‑83 (MQ=255)
ccATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg < 2:1180306/83‑1 (MQ=255)
aTCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgc > 1:138673/1‑139 (MQ=255)
ttCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcgcg < 1:258378/139‑1 (MQ=255)
gATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCtttt < 2:1124952/139‑1 (MQ=255)
gATGACGATCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCCGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCtttt < 1:740062/139‑1 (MQ=255)
gTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGcc < 2:583488/139‑1 (MQ=255)
tCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCt < 1:402337/139‑1 (MQ=255)
caatatggtgaggtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcg > 2:629157/12‑117 (MQ=255)
caatatggtgaggtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcg < 1:629157/106‑1 (MQ=255)
ccATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGt > 2:411027/1‑139 (MQ=255)
ggtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg > 1:1135073/1‑49 (MQ=255)
ggtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg < 2:1135073/49‑1 (MQ=255)
ggtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGc < 2:1074888/139‑1 (MQ=255)
ggtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGc < 2:285590/139‑1 (MQ=255)
gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCg > 1:78249/1‑139 (MQ=255)
caggtcaCCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAg > 1:250859/1‑139 (MQ=255)
aggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg > 1:331529/1‑45 (MQ=255)
aggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg < 2:331529/45‑1 (MQ=255)
ggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGtt > 1:973161/1‑139 (MQ=255)
aCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTgg < 2:78249/139‑1 (MQ=255)
cGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTAGTAGGTCTGCTTTGGCGAGTTCTTGaa < 2:828105/139‑1 (MQ=255)
cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcgcg < 1:780736/94‑1 (MQ=255)
cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcgcg > 2:780736/1‑94 (MQ=255)
cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcgcg < 2:779151/94‑1 (MQ=255)
cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcgcg > 1:779151/1‑94 (MQ=255)
cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCgg > 2:708340/1‑139 (MQ=255)
gAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCa < 1:411027/139‑1 (MQ=255)
gAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCa < 1:965500/139‑1 (MQ=255)
|
TTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCA > NC_000913/649245‑649497
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
