Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	711,724	C→T	A59T (GCG→ACG)	ybfE ←	LexA‑regulated protein, CopB family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	711,724	0	C	T	100.0%	153.7 / NA	49	A59T (GCG→ACG)	ybfE	LexA‑regulated protein, CopB family
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/22); total (27/22)

ATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGC  >  NC_000913/711591‑711854

aTGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCa                                                                                                                               >  1:480937/1‑139 (MQ=255)

             tttAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                                  <  2:381603/139‑1 (MQ=255)

             tttAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                                  <  1:604504/139‑1 (MQ=255)

             tttAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                                  <  1:24795/139‑1 (MQ=255)

                                 cGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGACACGTACtt                                                                                              >  2:779605/1‑139 (MQ=255)

                                   cAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTg                                                                                            >  1:691603/1‑139 (MQ=255)

                                      cgccgcCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTcg                                                                                         >  1:697130/1‑139 (MQ=255)

                                      cgccgcCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTcg                                                                                         >  1:526935/1‑139 (MQ=255)

                                       gccgccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTcgc                                                                                        >  1:310855/1‑139 (MQ=255)

                                        ccgccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTcgcg                                                                                       >  2:736905/1‑139 (MQ=255)

                                                  ctgcATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTgg                                                                             <  2:691603/139‑1 (MQ=255)

                                                     cATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTg                                                                          >  1:1040428/1‑139 (MQ=255)

                                                          aCATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                        >  1:207387/1‑136 (MQ=255)

                                                          aCATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                        <  2:207387/136‑1 (MQ=255)

                                                              ctctTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTttatta                                                                 <  1:940569/139‑1 (MQ=255)

                                                               tctTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTaa                                                                >  2:1001114/1‑139 (MQ=255)

                                                                 ttCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATa                                                              <  2:999478/139‑1 (MQ=255)

                                                                   cGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACg                                                            <  1:252759/139‑1 (MQ=255)

                                                                      tCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGtt                                                                      <  1:522137/126‑1 (MQ=255)

                                                                      tCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGtt                                                                      >  2:522137/1‑126 (MQ=255)

                                                                        aGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGtt                                                                           >  1:311000/1‑119 (MQ=255)

                                                                        aGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGtt                                                                           <  2:311000/119‑1 (MQ=255)

                                                                         gTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTg                                                      >  2:85581/1‑139 (MQ=255)

                                                                          ttgggtACGGCGCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGt                                                     >  1:708056/6‑139 (MQ=255)

                                                                           tCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGtt                                                    >  2:629964/1‑139 (MQ=255)

                                                                            cGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTc                                                   >  2:770876/1‑139 (MQ=255)

                                                                               tACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGtt                                                                           >  1:898152/1‑112 (MQ=255)

                                                                               tACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGtt                                                                           <  2:898152/112‑1 (MQ=255)

                                                                               tACGGCCCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCCGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATc                                                >  1:334265/1‑139 (MQ=255)

                                                                                  ggCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                        <  1:653944/112‑1 (MQ=255)

                                                                                  ggCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                        >  2:653944/1‑112 (MQ=255)

                                                                                      cATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGaa                                         <  2:133219/139‑1 (MQ=255)

                                                                                        tatTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAg                                       >  2:19939/1‑139 (MQ=255)

                                                                                         atTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGaaa                                        >  2:502221/1‑137 (MQ=255)

                                                                                         atTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGaaa                                        <  1:502221/137‑1 (MQ=255)

                                                                                             cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGtt                                                                      >  2:937956/1‑103 (MQ=255)

                                                                                             cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGtt                                                                      <  1:937956/103‑1 (MQ=255)

                                                                                             cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGAt                                  >  2:1046269/1‑139 (MQ=255)

                                                                                             cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGAt                                  >  1:773205/1‑139 (MQ=255)

                                                                                             cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGAt                                  >  1:156577/1‑139 (MQ=255)

                                                                                             cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGAt                                  <  2:994858/139‑1 (MQ=255)

                                                                                                  cTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGtt                             >  2:367526/1‑139 (MQ=255)

                                                                                                      gCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCg                         <  1:1046269/139‑1 (MQ=255)

                                                                                                      gCCAGCTCGTTCAGCGCCTCCACCGCTTCCGTGTTCAGCTTCATTTCGACACGCTTAAGGCCACGTACTTTTTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTTTTCATCGCGCGAAAGCGGATTAGTTTTCg                         <  1:19939/139‑1 (MQ=255)

                                                                                                          gCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGgtcg                     <  2:708056/139‑1 (MQ=255)

                                                                                                               ttCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTcc                  >  2:436593/1‑137 (MQ=255)

                                                                                                               ttCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTcc                  <  1:436593/137‑1 (MQ=255)

                                                                                                                    cgcCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGa           <  1:85581/139‑1 (MQ=255)

                                                                                                                             aGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTgc  <  1:629964/139‑1 (MQ=255)

ATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGC  >  NC_000913/711591‑711854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A