Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,621,307	A→G	E37E (GAA→GAG)	purM →	phosphoribosylaminoimidazole synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,621,307	0	A	G	100.0%	152.6 / NA	44	E37E (GAA→GAG)	purM	phosphoribosylaminoimidazole synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/20); total (24/20)

TGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACAC  >  NC_000913/2621183‑2621441

tGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCt                                                                                                                          >  2:313863/1‑139 (MQ=255)

                 aCCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcgc                                                                                                         >  2:852557/1‑139 (MQ=255)

                           ccTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCAtt                                                                                               <  2:179965/139‑1 (MQ=255)

                                ctTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTg                                                                                                            >  2:701791/1‑121 (MQ=255)

                                ctTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTg                                                                                                            <  1:701791/121‑1 (MQ=255)

                                      tACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          <  1:1000807/117‑1 (MQ=255)

                                      tACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          >  2:1000807/1‑117 (MQ=255)

                                           aGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGt                                                                               >  2:831215/1‑139 (MQ=255)

                                             aTGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGCAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGa                                                                             >  1:1127072/1‑139 (MQ=255)

                                               gCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAAc                                                                           >  1:604226/1‑139 (MQ=255)

                                                           atTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGttt                                                               >  2:585336/1‑139 (MQ=255)

                                                              gACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                             <  2:423369/90‑1 (MQ=255)

                                                              gACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                             >  1:423369/1‑90 (MQ=255)

                                                              gACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTg                                                            >  2:31731/1‑139 (MQ=255)

                                                                cgcgGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGc                                                          >  2:561349/1‑139 (MQ=255)

                                                                 gcgGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCa                                                         <  2:313771/139‑1 (MQ=255)

                                                                    ggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTg                                                      >  2:127415/1‑139 (MQ=255)

                                                                     gTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGa                                                     <  2:604226/139‑1 (MQ=255)

                                                                        aTGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACgg                                                  <  1:585336/139‑1 (MQ=255)

                                                                         tGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGc                                                 >  2:1217397/1‑139 (MQ=255)

                                                                         tGCTCTGGTTGGAAGAATC‑AAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTg                                                                   <  1:716956/120‑1 (MQ=255)

                                                                         tGCTCTGGTTGGAAGAATC‑AAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTg                                                                   >  2:716956/1‑120 (MQ=255)

                                                                               ggTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                             >  1:1094353/1‑73 (MQ=255)

                                                                               ggTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                             <  2:1094353/73‑1 (MQ=255)

                                                                               ggTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGt                                           >  2:900774/1‑139 (MQ=255)

                                                                               ggTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGt                                           >  2:820117/1‑139 (MQ=255)

                                                                                gTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTa                                          >  2:153774/1‑139 (MQ=255)

                                                                                   ggAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCaaaaa                                                                                     >  1:380642/1‑93 (MQ=255)

                                                                                   ggAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCaaaaa                                                                                     <  2:380642/93‑1 (MQ=255)

                                                                                               ggCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTg                                                      <  2:285622/112‑1 (MQ=255)

                                                                                               ggCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTg                                                      >  1:285622/1‑112 (MQ=255)

                                                                                                   tAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          >  1:955610/1‑56 (MQ=255)

                                                                                                   tAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          <  2:955610/56‑1 (MQ=255)

                                                                                                             aaCGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTaaaa             <  1:852557/139‑1 (MQ=255)

                                                                                                                  gtcgtcCGTAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCg                                                                                >  2:790535/1‑67 (MQ=255)

                                                                                                                  gtcgtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCg                                                                                <  1:790535/67‑1 (MQ=255)

                                                                                                                  gtcgtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCa        >  1:477841/1‑139 (MQ=255)

                                                                                                                   tcgtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCAc       <  1:31731/139‑1 (MQ=255)

                                                                                                                   tcgtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCAc       <  1:1217397/139‑1 (MQ=255)

                                                                                                                     gtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGc                                                                                             >  1:168679/1‑51 (MQ=255)

                                                                                                                     gtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGc                                                                                             <  2:168679/51‑1 (MQ=255)

                                                                                                                     gtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGa     <  1:313863/139‑1 (MQ=255)

                                                                                                                        cGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGacac  <  1:900774/139‑1 (MQ=255)

                                                                                                                        cGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGacac  <  1:153774/139‑1 (MQ=255)

TGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACAC  >  NC_000913/2621183‑2621441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A