Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,077,469	G→A	L7L (TTG→TTA)	yiiD →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,077,469	0	G	A	100.0%	124.8 / NA	39	L7L (TTG→TTA)	yiiD	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/20); total (19/20)

TTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTGTCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCAAAAGGTTCGGAAC  >  NC_000913/4077339‑4077602

ttCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGa                                                                                                                               <  1:292123/139‑1 (MQ=255)

         cGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAagaga                                                                                                                      <  2:282116/139‑1 (MQ=255)

            tGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAagagagag                                                                                                                   <  1:952778/139‑1 (MQ=255)

                        gagaTGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                                             >  1:1080023/1‑133 (MQ=255)

                        gagaTGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                                             <  2:1080023/133‑1 (MQ=255)

                        gagaTGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTa                                                                                                       >  1:123433/1‑139 (MQ=255)

                            tGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                                  >  2:898748/1‑108 (MQ=255)

                            tGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                                  <  1:898748/108‑1 (MQ=255)

                                             cGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACggg                                                                                                                                >  2:867749/1‑93 (MQ=255)

                                             cGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACggg                                                                                                                                <  1:867749/93‑1 (MQ=255)

                                                ttCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACaga                                                                               <  2:540806/139‑1 (MQ=255)

                                                   gCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaaga                                                                            >  1:1055395/1‑139 (MQ=255)

                                                    cTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGGGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaagaa                                                                           <  2:1055395/139‑1 (MQ=255)

                                                        ggATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAAt                                                                       <  2:123433/139‑1 (MQ=255)

                                                                 ggTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaag                                                                             <  2:372447/124‑1 (MQ=255)

                                                                 ggTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaag                                                                             >  1:372447/1‑124 (MQ=255)

                                                                    aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTaga                                                                   <  2:1157817/131‑1 (MQ=255)

                                                                    aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTaga                                                                   >  1:1157817/1‑131 (MQ=255)

                                                                       gCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATc                                                        >  2:639224/1‑139 (MQ=255)

                                                                        cTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCa                                                                                                                                    >  2:203364/1‑62 (MQ=255)

                                                                        cTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCa                                                                                                                                    <  1:203364/62‑1 (MQ=255)

                                                                        cTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTAt                                                                                                      >  1:242527/1‑92 (MQ=255)

                                                                        cTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTAt                                                                                                      <  2:242527/92‑1 (MQ=255)

                                                                                     gCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGaa                                                                                                                              >  1:709503/1‑55 (MQ=255)

                                                                                     gCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGaa                                                                                                                              <  2:709503/55‑1 (MQ=255)

                                                                                     gCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACaaa                                                                                   >  2:57086/1‑98 (MQ=255)

                                                                                     gCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACaaa                                                                                   <  1:57086/98‑1 (MQ=255)

                                                                                     gCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGaa                                          >  2:103140/1‑139 (MQ=255)

                                                                                        ccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATg                                       <  1:103140/139‑1 (MQ=255)

                                                                                                ttCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTaa                               >  1:540048/1‑139 (MQ=255)

                                                                                                       tGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGccc                           <  2:974824/136‑1 (MQ=255)

                                                                                                       tGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGcac                           >  1:974824/1‑134 (MQ=255)

                                                                                                                 aGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCg                                                                >  2:176827/1‑89 (MQ=255)

                                                                                                                 aGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCg                                                                <  1:176827/89‑1 (MQ=255)

                                                                                                                 aGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCaa              <  1:639224/139‑1 (MQ=255)

                                                                                                                          ccAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCAAAAGGTTCgg     >  2:813760/1‑139 (MQ=255)

                                                                                                                            aGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCTTa                                >  2:1156558/1‑110 (MQ=255)

                                                                                                                            aGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTa                                <  1:1156558/110‑1 (MQ=255)

                                                                                                                             gggTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCAAAAGGTTCGGAac  >  2:1162437/1‑139 (MQ=255)

TTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTGTCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCAAAAGGTTCGGAAC  >  NC_000913/4077339‑4077602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A