Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I2 R1 780 68.3 3343050 93.5% 3125751 102.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 960,165 (T)8→9 intergenic (+70/‑99) aroA → / → ycaL 5‑enolpyruvylshikimate‑3‑phosphate synthetase/putative peptidase‑related chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913960,1571.T97.7% 271.1 / ‑1.1 85intergenic (+62/‑107)aroA/ycaL5‑enolpyruvylshikimate‑3‑phosphate synthetase/putative peptidase‑related chaperone
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (39/44);  total (39/46)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

TCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTG  >  NC_000913/960020‑960287
                                                                                                                                          |                                                                                                                                   
tCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑‑t                                                                                                                                   >  1:1337300/1‑139 (MQ=255)
  ttGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑‑ttt                                                                                                                                 >  2:650762/1‑139 (MQ=255)
          aaaTGCGCGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑‑tttttttttcg                                                                                                                         >  2:605485/1‑136 (MQ=255)
           aaTGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑‑TTTTTTTTCGGa                                                                                                                        <  2:1337300/139‑1 (MQ=255)
                 cGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAATTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTtg                                                                                                                   >  1:696681/1‑139 (MQ=255)
                 cGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTtg                                                                                                                   >  2:32475/1‑139 (MQ=255)
                  ggCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGAc                                                                                                                       >  2:1401855/1‑134 (MQ=255)
                  ggCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGAc                                                                                                                       <  1:1401855/134‑1 (MQ=255)
                  ggCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTtgt                                                                                                                  <  1:1453987/139‑1 (MQ=255)
                    ccAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGAcc                                                                                                                      >  1:638112/1‑133 (MQ=255)
                    ccAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGAcc                                                                                                                      <  2:638112/133‑1 (MQ=255)
                    ccAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGa                                                                                                                >  1:1400489/1‑139 (MQ=255)
                                  gATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTaa                                                                                                  >  1:1452259/1‑139 (MQ=255)
                                   attattTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCtt                                                                                                                    <  1:1500943/120‑1 (MQ=255)
                                   attattTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCtt                                                                                                                    >  2:1500943/1‑120 (MQ=255)
                                   attattTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAAt                                                                                                 >  2:1277736/1‑139 (MQ=255)
                                   attattCCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTTTCGGACCTTGTGAGTCATTTTGATTaa                                                                                                  >  2:928100/1‑139 (MQ=255)
                                       tttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTa                                                                                                   <  2:1443134/133‑1 (MQ=255)
                                       tttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTa                                                                                                   >  1:1443134/1‑133 (MQ=255)
                                        ttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAg                                                                                            <  1:32475/139‑1 (MQ=255)
                                        ttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑‑ttt                                                                                                                                 <  1:1374277/101‑1 (MQ=255)
                                        ttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑‑ttt                                                                                                                                 >  2:1374277/1‑101 (MQ=255)
                                            agcagcTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTc                                                                                        >  1:535495/1‑139 (MQ=255)
                                            agcagcTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCg                                                                                       <  1:731610/139‑1 (MQ=255)
                                             gcagcTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCg                                                                                       <  2:50426/139‑1 (MQ=255)
                                                 cTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCAtt                                                                                                          <  2:137896/116‑1 (MQ=255)
                                                 cTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCAtt                                                                                                          >  1:137896/1‑116 (MQ=255)
                                                 cTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTg                                                                                   <  2:1400489/139‑1 (MQ=255)
                                                  tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGt                                                                                  >  2:143797/1‑139 (MQ=255)
                                                        ggATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGt                                                                            <  2:1452259/139‑1 (MQ=255)
                                                         gATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTa                                                                           >  2:1250315/1‑139 (MQ=255)
                                                           ttAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAg                                                                         <  1:143797/139‑1 (MQ=255)
                                                               ccAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttgt                                                                     <  2:535495/139‑1 (MQ=255)
                                                                cAGGCAGCCTGAATGAACAACGGGCAATAACTAGCCAAATCTTTCTTTATCAAAACGTCGGTACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTATCGTCGCTTGTCAATGTAAgttgtt                                                                    <  1:605485/139‑1 (MQ=255)
                                                                 aggaaacaggaatgaaCAACGGGAAAAAAATAGCCAAATCTTTCTTTATCAAACGGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGttgttg                                                                   <  1:803740/130‑1 (MQ=255)
                                                                 aGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAATTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGttgttg                                                                   <  2:696681/139‑1 (MQ=255)
                                                                    cAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTg                                                                                   <  2:967647/121‑1 (MQ=255)
                                                                    cAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTg                                                                                   >  1:967647/1‑121 (MQ=255)
                                                                    cAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTg                                                                                                       <  2:180156/100‑1 (MQ=255)
                                                                    cAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTg                                                                                                       >  1:180156/1‑100 (MQ=255)
                                                                      gCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACa                                                              <  1:650762/139‑1 (MQ=255)
                                                                       ccTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGt                                                                                  >  2:1236513/1‑118 (MQ=255)
                                                                       ccTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACat                                                             >  2:165717/1‑139 (MQ=255)
                                                                       ccTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTAGCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGt                                                                                  <  1:1236513/118‑1 (MQ=255)
                                                                        cTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTg                                                                                                       >  2:1127435/1‑96 (MQ=255)
                                                                        cTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTg                                                                                                       <  1:1127435/96‑1 (MQ=255)
                                                                        cTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACata                                                            <  2:900455/139‑1 (MQ=255)
                                                                        cTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACata                                                            <  2:1614964/139‑1 (MQ=255)
                                                                        cTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACata                                                            <  1:1250315/139‑1 (MQ=255)
                                                                         tgaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTaa                                                                                                  >  1:863052/1‑100 (MQ=255)
                                                                         tgaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTaa                                                                                                  <  2:863052/100‑1 (MQ=255)
                                                                             tgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATAtt                                                       >  2:739219/1‑139 (MQ=255)
                                                                                 caacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATa                                                                <  1:1035809/126‑1 (MQ=255)
                                                                                 caacGGGCAATAAATAGCCAAATCTTTCCTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATa                                                                >  2:1035809/1‑126 (MQ=255)
                                                                                     gggCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTTGGACCTTGTGAGTCATTTTGATTTATGGTAGCGTCGCTTGTTAATGTAAGTTGTTTATACATAATAtttttttctt                                               >  2:803740/1‑132 (MQ=255)
                                                                                     gggCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATg                                               >  1:1596583/1‑139 (MQ=255)
                                                                                      ggCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTg                                                                                                       <  2:1031758/82‑1 (MQ=255)
                                                                                      ggCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGa                                              <  1:739219/139‑1 (MQ=255)
                                                                                      ggCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCAAATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTg                                                                                                       >  1:1031758/1‑82 (MQ=255)
                                                                                       gCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGAt                                             <  1:1277736/139‑1 (MQ=255)
                                                                                        cAATAAATTGCCAAATTTTTCTTTAACAAAACGTCGGCAAATTGTCGGCGTTTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGAt                                             <  1:928100/139‑1 (MQ=255)
                                                                                           taaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAAt                                         <  1:648223/139‑1 (MQ=255)
                                                                                                aGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttgt                                                                     <  2:1623185/107‑1 (MQ=255)
                                                                                                aGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttgt                                                                     >  1:1623185/1‑107 (MQ=255)
                                                                                                 gCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAg                                                                                            >  1:204865/1‑82 (MQ=255)
                                                                                                 gCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAg                                                                                            <  2:204865/82‑1 (MQ=255)
                                                                                                 gCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgt                                                                        >  2:1420539/1‑102 (MQ=255)
                                                                                                 gCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgt                                                                        <  1:1420539/102‑1 (MQ=255)
                                                                                                     aaTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCg                                                                                       >  1:803821/1‑83 (MQ=255)
                                                                                                     aaTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCg                                                                                       <  2:803821/83‑1 (MQ=255)
                                                                                                     aaTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGc                                                                                      >  2:1458471/1‑84 (MQ=255)
                                                                                                     aaTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGc                                                                                      <  1:1458471/84‑1 (MQ=255)
                                                                                                      aTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGAt                                                                                                     >  2:177696/1‑68 (MQ=255)
                                                                                                      aTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGAt                                                                                                     <  1:177696/68‑1 (MQ=255)
                                                                                                      aTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTaa                                                                          <  1:272349/95‑1 (MQ=255)
                                                                                                      aTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTaa                                                                          >  2:272349/1‑95 (MQ=255)
                                                                                                       tctttcttTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGttgttg                                                                   >  2:9738/1‑101 (MQ=255)
                                                                                                       tctttcttTATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGttgttg                                                                   <  1:9738/101‑1 (MQ=255)
                                                                                                               tATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCAtt                                                                                                          >  1:1373525/1‑54 (MQ=255)
                                                                                                               tATCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCAtt                                                                                                          <  2:1373525/54‑1 (MQ=255)
                                                                                                                aTCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttgt                                                                     <  2:1051111/90‑1 (MQ=255)
                                                                                                                aTCAAAACGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttgt                                                                     >  1:1051111/1‑90 (MQ=255)
                                                                                                                     aaCGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGt                                                                                  >  2:1162887/1‑72 (MQ=255)
                                                                                                                     aaCGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGt                                                                                  <  1:1162887/72‑1 (MQ=255)
                                                                                                                      aCGTCGGCACATTGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAAACAACGGATGATTCACATGAAGAATACt              <  2:1596583/139‑1 (MQ=255)
                                                                                                                                  tGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCAtt                                                                                                          >  1:1324704/1‑35 (MQ=255)
                                                                                                                                  tGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCAtt                                                                                                          <  2:1324704/35‑1 (MQ=255)
                                                                                                                                  tGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCg                                                                                       >  2:150394/1‑54 (MQ=255)
                                                                                                                                  tGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCg                                                                                       <  1:150394/54‑1 (MQ=255)
                                                                                                                                  tGTCGGCGT‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTActgctg  >  2:1426156/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                   
TCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑‑TTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTG  >  NC_000913/960020‑960287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A