Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I2 R1
|
780 |
68.3 |
3343050 |
93.5% |
3125751 |
102.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,270,228 |
C→T |
intergenic (+305/+10) |
rdlB → / ← ldrC |
sRNA antisense regulator affects LdrB translation, proposed addiction module in LDR‑B repeat, with toxic peptide LdrB/toxic polypeptide, small |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,270,228 | 0 | C | T | 100.0%
| 54.5
/ NA
| 23 | intergenic (+305/+10) | rdlB/ldrC | sRNA antisense regulator affects LdrB translation, proposed addiction module in LDR‑B repeat, with toxic peptide LdrB/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/11); total (12/11) |
AGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGG > NC_000913/1270095‑1270297
|
aGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGtttt < 2:1149813/139‑1 (MQ=25)
ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCAcc < 2:666752/139‑1 (MQ=25)
tctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCt > 1:554494/1‑139 (MQ=21)
ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCAc < 1:1374699/123‑1 (MQ=18)
ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCAc > 2:1374699/1‑123 (MQ=18)
ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACg > 1:1074840/1‑139 (MQ=17)
cTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGccac < 2:554494/139‑1 (MQ=18)
cGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGccacca > 2:1615796/1‑139 (MQ=17)
ggTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGc > 2:685872/1‑139 (MQ=18)
tACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGc < 1:685872/139‑1 (MQ=25)
tgGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGtt > 2:464715/1‑91 (MQ=21)
tgGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGtt < 1:464715/91‑1 (MQ=21)
tgGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGtaat > 1:627555/1‑139 (MQ=25)
ggAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCt < 1:218739/130‑1 (MQ=37)
ggAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCt > 2:218739/1‑130 (MQ=37)
ggAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGtaata > 2:1468987/1‑139 (MQ=37)
aaaGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGcc > 2:482877/1‑139 (MQ=18)
aaaGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGcc > 1:324201/1‑139 (MQ=17)
aCAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGa < 2:270267/139‑1 (MQ=17)
aaaTGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAAtt < 1:188657/125‑1 (MQ=17)
aaaTGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAAtt > 2:188657/1‑125 (MQ=18)
aaaTGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCgg < 1:1468987/139‑1 (MQ=18)
aaaTGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCTGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCgg < 1:482877/139‑1 (MQ=17)
|
AGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGG > NC_000913/1270095‑1270297
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
