Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A4 F1 I2 R1 | 780 | 68.3 | 3343050 | 93.5% | 3125751 | 102.9 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,803,711 | T→C | pseudogene (194/1476 nt) | arpB → | pseudogene, ankyrin repeats |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,803,711 | 0 | T | C | 100.0% | 239.0 / NA | 67 | pseudogene (194/1476 nt) | arpB | pseudogene, ankyrin repeats |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/34); total (33/34) | |||||||||||
TTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGTCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTGTCGAATGATGGCAAACAATGTCAGTC > NC_000913/1803575‑1803845 | tttttGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGccc > 2:790353/1‑139 (MQ=255) tttGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCcccc < 1:790353/139‑1 (MQ=255) tttGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCcccc < 2:497967/139‑1 (MQ=255) ttGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCt < 1:1235875/139‑1 (MQ=255) tGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCtt > 2:1108944/1‑139 (MQ=255) aTCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTc > 2:975137/1‑139 (MQ=255) tGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGAcgcg < 1:570097/139‑1 (MQ=255) tGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGAcgcg < 2:1534473/139‑1 (MQ=255) cTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCACCCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGtt < 1:639486/139‑1 (MQ=255) tATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGAcgcg < 2:512313/133‑1 (MQ=255) tATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGAcgcg > 1:512313/1‑133 (MQ=255) tGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCa < 2:298246/139‑1 (MQ=255) cAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTg > 1:452783/1‑139 (MQ=255) aTACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGtt < 2:1398954/111‑1 (MQ=255) aTACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGtt > 1:1398954/1‑111 (MQ=255) aCGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAgg > 2:500618/1‑139 (MQ=255) ccTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGt > 1:551291/1‑139 (MQ=255) ttGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTcg > 2:826938/1‑139 (MQ=255) tGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGAcgcg > 1:294481/1‑97 (MQ=255) tGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGAcgcg < 2:294481/97‑1 (MQ=255) cATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTAt < 1:826938/139‑1 (MQ=255) ttGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCg < 2:706575/83‑1 (MQ=255) ttGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCg > 1:706575/1‑83 (MQ=255) ttGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATcg < 2:602773/139‑1 (MQ=255) gAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGa > 1:1255760/1‑139 (MQ=255) aaGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAg > 1:1161751/1‑115 (MQ=255) aaGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAg < 2:1161751/115‑1 (MQ=255) aaaGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCt < 1:500618/139‑1 (MQ=255) aaaGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCt < 2:1255760/139‑1 (MQ=255) aaGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGTAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCtt < 1:975137/139‑1 (MQ=255) ccTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTcc > 1:1535289/1‑56 (MQ=255) ccTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTcc < 2:1535289/56‑1 (MQ=255) ccTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTa > 2:855668/1‑139 (MQ=255) tAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCATTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCTCTTTAGATAtt > 2:1093603/1‑139 (MQ=255) aCATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTc > 2:689793/1‑139 (MQ=255) cATTACTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCt < 2:551291/139‑1 (MQ=255) aCTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGAc > 2:1263021/1‑47 (MQ=255) aCTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGAc < 1:1263021/47‑1 (MQ=255) aCTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCtt < 2:921414/118‑1 (MQ=255) aCTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTg > 2:1306645/1‑139 (MQ=255) aCTCCTCCGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTAAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCtt > 1:921414/1‑118 (MQ=255) ctccGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGt > 1:439640/1‑100 (MQ=255) ctccGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGt < 2:439640/100‑1 (MQ=255) ctccGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGata > 2:727395/1‑125 (MQ=255) ctccGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGata < 1:727395/125‑1 (MQ=255) cGTTGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTGTCGAatg > 2:67625/1‑139 (MQ=255) ttGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGAcgcg > 1:732765/1‑41 (MQ=255) ttGAATCCATACAGCAGCATGCCCCCTTAGTCCGGGAcgcg < 2:732765/41‑1 (MQ=255) gAATCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTGTCGAATGATgg < 1:689793/139‑1 (MQ=255) aaTCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCAtcctc < 2:149625/66‑1 (MQ=255) aaTCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCAtcctc > 1:149625/1‑66 (MQ=255) tCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCtt < 2:1126101/122‑1 (MQ=255) tCCATACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCtt > 1:1126101/1‑122 (MQ=255) aTACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTc < 2:168747/117‑1 (MQ=255) aTACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTc > 1:168747/1‑117 (MQ=255) tACAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTGTCGAATGATGGCAAACaa > 1:123359/1‑139 (MQ=255) aCAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCtt > 1:876697/1‑99 (MQ=255) aCAGCAGCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCtt < 2:876697/99‑1 (MQ=255) cagcagCATGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTGTCGAATGATGGCAAACAATg < 2:123359/139‑1 (MQ=255) gcagcaTGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTGTCGAATGATGGCa > 2:268600/1‑130 (MQ=255) gcagcaTGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTGTCGAATGATGGCa < 1:268600/130‑1 (MQ=255) cagcaTGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTa > 1:987025/1‑82 (MQ=255) cagcaTGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTa < 2:987025/82‑1 (MQ=255) caTGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATcgc < 2:1288599/74‑1 (MQ=255) caTGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATcgc > 1:1288599/1‑74 (MQ=255) caTGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTGTCGAATGATGGCaaa < 1:475674/128‑1 (MQ=255) caTGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTGTCGAATGATGGCAAACAATgtcagtc > 1:623844/1‑139 (MQ=255) caTGCCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTCCGCTTTAGATATTTTCTTTTGTCGAATGATGGCaaa > 2:475674/1‑128 (MQ=255) | TTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACAGCAGCATGTCCCCTTAGTCCGGGACGCGGAGTTCAGAGCATTACTTCGCCATCCTCCTGCAGGGAGTCGCGTTATCGCGAGTAAGGATTTTGGCTTCGCTTTAGATATTTTCTTTTGTCGAATGATGGCAAACAATGTCAGTC > NC_000913/1803575‑1803845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |