Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A4 F1 I2 R1 | 780 | 68.3 | 3343050 | 93.5% | 3125751 | 102.9 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,378,777 | (C)6→7 | coding (483/1671 nt) | menD ← | 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase, SEPHCHC synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,378,771 | 1 | . | C | 100.0% | 168.2 / NA | 48 | G163G (GGA→GGG) | menD | 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase, SEPHCHC synthase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (24/24); total (24/24) | |||||||||||
TTTCCAGACGAGGCGCTTCACGCAGCCACGGTTTGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACT‑CCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAA > NC_000913/2378638‑2378901 | tttCCAGACGAGGCGCTTCACGCAGCCACGGTTTGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACT‑ccccc < 1:595359/139‑1 (MQ=255) gcgcTTCACGCAGCCACGGTTTGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAGCGGGCAGTTGATATGGACT‑cccccccgc < 2:73141/131‑4 (MQ=255) gcgcTTCACGCAGCCACGGTTTGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAGCGGGCAGTTGATATGGACT‑cccccccgc > 1:73141/1‑128 (MQ=255) gcgcTTCACGCAGCCACGGTTTGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCg > 2:986156/1‑139 (MQ=255) aGCCACGGTTTGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCg > 2:352852/1‑139 (MQ=255) gTTTGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGa > 1:570532/1‑139 (MQ=255) tttGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACT‑cccccc > 2:530078/1‑109 (MQ=255) tttGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACT‑cccccc < 1:530078/109‑1 (MQ=255) tGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATgg > 1:670243/1‑139 (MQ=255) ccTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCg > 1:997056/1‑112 (MQ=255) ccTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCg < 2:997056/112‑1 (MQ=255) tGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGt < 1:1034408/125‑1 (MQ=255) tGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGt > 2:1034408/1‑125 (MQ=255) tGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAAcc < 1:986156/139‑1 (MQ=255) ccaGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCa < 2:1453081/139‑1 (MQ=255) ccaGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCa < 2:1197890/139‑1 (MQ=255) cACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTg < 1:556386/139‑1 (MQ=255) cccAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCg > 2:995996/1‑139 (MQ=255) aGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCgggg < 1:352852/139‑1 (MQ=255) aGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCgggg < 2:670243/139‑1 (MQ=255) aGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCgggg < 2:570532/139‑1 (MQ=255) gCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGt < 1:1130272/106‑1 (MQ=255) gCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGt > 2:1130272/1‑106 (MQ=255) ttGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCg > 1:26355/1‑98 (MQ=255) ttGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCg < 2:26355/98‑1 (MQ=255) tAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGgc > 1:1022546/1‑139 (MQ=255) tAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACT‑cccc < 2:943857/66‑1 (MQ=255) tAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACT‑cccc > 1:943857/1‑66 (MQ=255) gTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCgg < 2:378454/135‑1 (MQ=255) gTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCgg > 1:378454/1‑135 (MQ=255) tATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCgaga < 1:1626403/78‑1 (MQ=255) tATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCgaga > 2:1626403/1‑78 (MQ=255) tCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCgggg < 1:1474762/109‑1 (MQ=255) tCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCgggg > 2:1474762/1‑109 (MQ=255) tCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACT‑cccccccgcat < 1:1503601/59‑6 (MQ=255) tCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACT‑cccccccgcat > 2:1503601/1‑54 (MQ=255) ccATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCgg > 1:375491/1‑120 (MQ=255) ccATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCgg < 2:375491/120‑1 (MQ=255) ccATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTg > 1:161013/1‑139 (MQ=255) aCAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGt < 2:1325459/74‑1 (MQ=255) aCAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGt > 1:1325459/1‑74 (MQ=255) aCAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGt < 2:161013/139‑1 (MQ=255) aCAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGt < 2:1277211/139‑1 (MQ=255) cGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGa < 2:1293866/44‑1 (MQ=38) cGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGa > 1:1293866/1‑44 (MQ=255) cGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGGAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCgggg > 2:1307897/1‑92 (MQ=255) cGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGGAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCgggg < 1:1307897/92‑1 (MQ=255) cGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTc > 2:498557/1‑64 (MQ=255) cGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTc < 1:498557/64‑1 (MQ=255) ttCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCgggg < 1:901592/89‑1 (MQ=255) ttCAGCAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCgggg > 2:901592/1‑89 (MQ=255) cAAACGGGCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACa > 1:340103/1‑139 (MQ=255) gggCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGt < 2:907121/50‑1 (MQ=255) gggCAGTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGt > 1:907121/1‑50 (MQ=255) gTTGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTg > 1:693999/1‑139 (MQ=255) ttGATATGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTgg > 2:715617/1‑139 (MQ=255) tatGGACTCCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGaa < 1:715617/139‑1 (MQ=255) | TTTCCAGACGAGGCGCTTCACGCAGCCACGGTTTGTCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACT‑CCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAA > NC_000913/2378638‑2378901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |